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All-trans-retinoic acid inhibits retinol esterification by acyl-CoA: retinol acyltransferase (EC 2.3.1.76) from rat and human small intestinal mucosa

Published online by Cambridge University Press:  09 March 2007

Müller Hanne  and
Kaare R. Norum
Müller Hanne
Affiliation:
Institute for Nutrition Research, University of Oslo, Oslo, Norway
Kaare R. Norum
Affiliation:
Institute for Nutrition Research, University of Oslo, Oslo, Norway
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Abstract

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1. Formation of retinyl esters catalyzed by acyl-CoA: retinol acyltransferase (ARAT; retinol fatty- acyltransferase; EC 2.3.1.76) from intestinal mucosa has been studied in vitro in the presence of all-trans-retinoic acid.

2. The incubation system contained microsomal preparations from the tissue tested, radioactive retinol and palmitoyl-CoA. The product formed was separated from the substrates by chromatography on alumina columns.

3. All-trans-retinoic acid inhibited ARAT both from rat and human intestinal mucosa.

4. Inhibition occurred instantly. At a concentration of retinol of 80, μM, a 50% inhibition was obtained with 50 μM- retinoic acid.

5. The inhibition of ARAT by retinoic acid may be of importance for normal retinol absorption in patients receiving retinoid therapy.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 55 , Issue 1 , January 1986 , pp. 37 - 41
Copyright
Copyright © The Nutrition Society 1986

References

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