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Aldose reductase (EC 1.1.1.21) activity and reduced-glutathione content in lenses of diabetic sand rats (Psammomys obesus) fed with acarbose

Published online by Cambridge University Press:  09 March 2007

Ester Cohen-Melamed ,
A. Nyska ,
A. Pollack  and
Z. Madar
Ester Cohen-Melamed
Affiliation:
Department of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
A. Nyska
Affiliation:
Kimron Veterinary Institute, Beit Dagan, Israel and Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Israel
A. Pollack
Affiliation:
Kaplan Hospital, Rehovot, Israel
Z. Madar
Affiliation:
Department of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
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Abstract

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The effects of acarbose on cataract development, lens aldose reductase (EC 1·1·1·21) activity and lenticular reduced-glutathione content in diabetic sand rats (Psammomys obesus) were determined. Diabetic sand rats (diet-induced) were fed on diets with or without acarbose(0.4 g/kg) for 39 d. Daily plasma glucose, cataract incidence, aldose reductase and glutathione content were evaluated. After 19 d on acarbose, daily plasma glucose profile was significantly reduced compared with that of sand rats not receiving acarbose. Cataract incidence was markedly lower in sand rats treated with acarbose. After 20 d, cataracts had developed in 90% of the animals fed without acarbose, whereas none was observed in sand rats fed with acarbose. After 37 d acarbose treatment the incidence of cataracts reached only 30%. Compared with untreated animals, lens aldose reductase activity was significantly lower in sand rats fed with acarbose for 39 d (7 6 (SE 0·78) v. 3·5 (SE 0·55) μmol NADPH/mg protein per min respectively, P < 0·001). Concomitantly, significantly higher lenticular protein and reduced-glutathione contents (90 (SE 23) v. 240 (SE 23.5) μg/mg tissue respectively, P < 0·001 and 369 (SE 48·6) v. 645 (SE 71·1)μg/mg tissue respectively, P < 0·001) were found. These results suggest that decreasing hyperglycaemia, accompanied by lower aldose reductase activity obtained by acarbose, led to a significant preventive effect on cataract development in sand rats.

Keywords

AcarboseCataractAldose reductaseSand rats
Type
Acarbose and cataract formation
Information
British Journal of Nutrition , Volume 74 , Issue 5 , November 1995 , pp. 607 - 615
Copyright
Copyright © The Nutrition Society 1995

References

Bancroft, J. D. & Stevenes, A. (1972). Theory and Practice of Histological Techniques. Edinburgh, London, New York: Churchill Livingstone.Google Scholar
Bischoff, H. (1993). Pharmacology of α-glucosidase inhibitors. In α-Glucosidase Inhibition: Potential Use in Diabetes. Drugs in Development, Vol. 1, pp. 3–13 [Vasselli, J. R., Maggio, C. A. and Scriabine, A. editors], Branford: Neva Press.Google Scholar
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Bunce, G. E., Kinoshita, J. H. & Horwitz, J. (1990). Nutritional factors in cataract. Annual Review of Nutrition 10, 233–254.Google Scholar
Chang, H. M., Fagerholm, P. & Chylack, L. T. J. (1983). Response of the lens to oxidative-osmotic stress. Experimental Eye Research 37, 11–21.Google Scholar
Clissold, S. P. & Edwards, C. (1988). Acarbose: a preliminary review of its pharmacodynamic and pharmacokinetic properties, and therapeutic potential. Drugs 35, 214–243.Google Scholar
Diabetes Control and Complication Trial Research Group (1993). The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. New England Journal of Medicine 329, 977–986.Google Scholar
Giblin, F. J., McCready, J. P. & Reddy, V. N. (1983). The role of glutathione metabolism in the detoxification of HO in rabbit lens. Investigative Ophthalmology and Visual Science 22, 330–335.Google Scholar
Gonzalez, A. M., Sochor, M. & McLean, P. (1983). The effect of an aldose reductase inhibitor (sorbinil) on the level of metabolism in lenses of diabetic rats. Diabetes 32, 482–485.CrossRefGoogle ScholarPubMed
Halder, A. B. & Crabbe, M. J. C. (1984). Bovine lens aldehyde reductase (aldose reductase): purification, kinetics and mechanism. Biochemical Journal 219, 33–39.Google Scholar
Hissin, P. J. & Hilf, R. (1976). A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analytical Biochemistry 74, 214–226.CrossRefGoogle ScholarPubMed
Hothersall, J. S., Taylor, C. E. & McLean, P. (1988). Antioxidant status in an in vitro model for hyperglycemic lens cataract formation: effect of aldose reductase inhibitor. Biochemical Medicine and Metabolic Biology 40, 109–117.Google Scholar
Kador, P. F., Akagi, Y. & Kinoshita, J. H. (1986). The effect of aldose reductase and its inhibition on sugar cataract formation. Metabolism 35, 15–19.Google Scholar
Kalman, R., Adler, J. H., Lazarovoci, G., Bar-On, H. & Ziv, E. (1993). The efficiency of sand rat metabolism is responsible for development of obesity and diabetes. Journal of Basic Clinical Physiology and Pharmacology 4, 57–68.Google Scholar
Kato, K., Nakayama, K., Ohta, M., Murakami, N., Murakami, K., Mizota, M., Miwa, I. & Okuda, J. (1990). Effect of novel hydantoin with aldose reductase inhibiting activity of galactose-induced cataract in rats. Japanese Journal of Pharmacology 54, 355–364.CrossRefGoogle ScholarPubMed
Kinoshita, J. H. (1986). Aldose reductase in the diabetic eye: XLIII Edward Jackson Memorial Lecture. American Journal of Ophthalmology 102, 685–692.Google Scholar
Krause, H. P., Ahr, H. J. & Boberg, M. (1993). Pharmacokinetics and metabolism of acarbose. In Drugs in Development, Vol. 1, pp. 15–23 [Vasselli, J. R., Maggio, C. A. and Scriabine, A., editors]. Branford: Neva Press.Google Scholar
Lee, S. M., Schade, S. Z. & Daughty, C. C. (1985). Aldose reductase, NADPH and NADP+ in normal, galactose-fed and diabetic rat lens. Biochimica et Biophysica Acta 841, 247–253.Google Scholar
Madar, Z. (1989). The effect of acarbose and miglitol (BAY-M-1099) on postprandial glucose levels following ingestion of various sources of starch by nondiabetic and streptozotocin-induced diabetic rats. Journal of Nutrition 119, 2023–2029.Google Scholar
Madar, Z. & Hazan, A. (1993a). Effects of acarbose and miglitol in vivo on carbohydrate digestion in type I diabetic, zucker obese and sand rats. In Drugs in Development, Vol. 1, pp. 95–107 [Vasselli, J. R., Maggio, C. A. and Scriabine, A., editors]. Branford: Neva Press.Google Scholar
Madar, Z. & Hazan, A. (1993b). Effect of miglitol and acarbose on starch digestion, daily plasma glucose profiles and cataract formation. Journal of Basic Clinical Physiology and Pharmacology 4, 69–81.CrossRefGoogle ScholarPubMed
Marquie, G., Duhault, J. & Jacotot, B. (1984). Diabetes mellitus in sand rats (Psammomys obesus). Diabetes 33, 438–443.Google Scholar
Morrison, B. (1972). Use of the Beckman glucose analyzer for low and high glucose values. Clinica Chimica Acta 42, 192–194.Google Scholar
Ohrloff, C, Hockwin, O., Olson, R. & Dickman, S. (1994). Glutathione peroxidase, glutathione reductase and superoxide dismutase in the aging lens. Current Eye Research 3, 109–115.CrossRefGoogle Scholar
Taylor, A. (1989). Associations between nutrition and cataract. Nutrition Reviews 47, 225–234.Google Scholar
Taylor, R. H. (1991). Alpha-glucosidase inhibitors. In New Antidiabetic Drugs, pp. 119–132 [Bailey, C. J. and Flatt, P. R., editors]. London: Smith-Gordon Co.Google Scholar
Zimmerman, B. R. (1992). Preventing long-term complications: implications for combination therapy with acarbose. Drugs 44, 54–66.CrossRefGoogle ScholarPubMed