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Adzuki resistant starch lowered serum cholesterol and hepatic 3-hydroxy-3-methylglutaryl-CoA mRNA levels and increased hepatic LDL-receptor and cholesterol 7α-hydroxylase mRNA levels in rats fed a cholesterol diet

Published online by Cambridge University Press:  08 March 2007

Kyu-Ho Han ,
Miharu Iijuka ,
Ken-ichiro Shimada ,
Mitsuo Sekikawa ,
Katsuhisa Kuramochi ,
Kiyoshi Ohba ,
Liyanage Ruvini ,
Hideyuki Chiji  and
Michihiro Fukushima
Kyu-Ho Han
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Miharu Iijuka
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Ken-ichiro Shimada
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Mitsuo Sekikawa
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Katsuhisa Kuramochi
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Kiyoshi Ohba
Affiliation:
Hokkaido Tokachi Area Regional Food Processing Technology Center, Obihiro, Hokkaido 080-2462, Japan
Liyanage Ruvini
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Hideyuki Chiji
Affiliation:
Department of Food Science and Human Nutrition, Faculty of Human Ecology, Fuji Women's College, Hanakawa minami 4-5, Ishikari, Hokkaido 061-3204, Japan
Michihiro Fukushima*
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
*
*Corresponding author: Dr Michihiro Fukushima, fax +81 155 49 5577, email [email protected]
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Abstract

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We examined the effects of adzuki bean resistant starch on serum cholesterol and hepatic mRNA in rats fed a cholesterol diet. The mRNA coded for key regulatory proteins of cholesterol metabolism. The control rats were fed 15 % cornstarch (basal diet, BD). The experimental rats were fed BD plus a 0·5 % cholesterol diet (CD), or a 15 % adzuki resistant starch plus 0·5 % cholesterol diet (ACD) for 4 weeks. The serum total cholesterol and VLDL + intermediate density lipoprotein + LDL-cholesterol levels in the ACD group were significantly lower than those in the CD group throughout the feeding period. The total hepatic cholesterol concentrations in the CD and ACD groups were not significantly different. The faecal total bile acid concentration in the ACD group was significantly higher than that in the BD and CD groups. Total SCFA and acetic acid concentrations in the ACD group were significantly higher than those in the CD group but there were no significant differences in the concentrations between the ACD and BD groups. The hepatic LDL-receptor mRNA and cholesterol 7α-hydroxylase mRNA levels in the ACD group were significantly higher than those in the CD group and the hepatic 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase mRNA level in the ACD group was significantly lower than in the CD group. The results suggest that adzuki resistant starch has a serum cholesterol-lowering function via enhancement of the hepatic LDL-receptor mRNA and cholesterol 7α-hydroxylase mRNA levels and faecal bile acid excretion, and a decrease in the hepatic HMG-CoA reductase mRNA level, when it is added to a cholesterol diet.

Keywords

Adzuki resistant starchSerum cholesterolShort-chain fatty acidBile acidHepatic mRNA
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 6 , December 2005 , pp. 902 - 908
Copyright
Copyright © The Nutrition Society 2005

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