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Adaptive responses in men fed low- and high-copper diets

Published online by Cambridge University Press:  07 June 2007

Linda J. Harvey ,
Gosia Majsak-Newman ,
Jack R. Dainty ,
D. John Lewis ,
Nicola J. Langford ,
Helen M. Crews  and
Susan J. Fairweather-Tait
Linda J. Harvey*
Affiliation:
Nutrition and Consumer Science Division, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Gosia Majsak-Newman
Affiliation:
Nutrition and Consumer Science Division, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Jack R. Dainty
Affiliation:
Nutrition and Consumer Science Division, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
D. John Lewis
Affiliation:
Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
Nicola J. Langford
Affiliation:
Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
Helen M. Crews
Affiliation:
Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
Susan J. Fairweather-Tait
Affiliation:
Nutrition and Consumer Science Division, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
*
*Corresponding author: Dr Linda Harvey, fax +44 1603 507723, email [email protected]
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Abstract

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The study of Cu metabolism is hampered by a lack of sensitive and specific biomarkers of status and suitable isotopic labels, but limited information suggests that Cu homeostasis is maintained through changes in absorption and endogenous loss. The aim of the present study was to employ stable-isotope techniques to measure Cu absorption and endogenous losses in adult men adapted to low, moderate and high Cu-supplemented diets. Twelve healthy men, aged 20–59 years, were given diets containing 0·7, 1·6 and 6·0 mg Cu/d for 8 weeks, with at least 4 weeks intervening washout periods. After 6 weeks adaptation, apparent and true absorption of Cu were determined by measuring luminal loss and endogenous excretion of Cu following oral administration of 3 mg highly enriched 65Cu stable-isotope label. Apparent and true absorption (41 and 48% respectively) on the low-Cu diet were not significantly different from the high-Cu diet (45 and 48% respectively). Endogenous losses were significantly reduced on the low- (0·45mg/d; P<0·001) and medium- (0·81 mg/d; P=0·001) compared with the high-Cu diet (2·46mg/d). No biochemical changes resulting from the dietary intervention were observed. Cu homeostasis was maintained over a wide range of intake and more rapidly at the lower intake, mainly through changes in endogenous excretion.

Keywords

CopperFructoseStable isotopesCopper absorptionCopper endogenous losses
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 1 , July 2003 , pp. 161 - 168
Copyright
Copyright © The Nutrition Society 2003

References

August, D, Janghorbani, M & Young, VR (1989) Determination of zinc and copper absorption at three different Zn-Cu ratios by using stable isotope methods in young and elderly subjects. Am J Clin Nutr 50, 1457–1463.CrossRefGoogle Scholar
Baxter, MJ, Crews, HM, Robb, P & Strutt, PR (1997) Quality control in the multi-element analysis of foods using ICP-MS. In Plasma Source Mass Spectrometry: Developments and Applications, Special Publication no. 202. pp 95–109. [Holland, G and Tanner, SC, editors]. Cambridge: Royal Society of Chemistry.Google Scholar
Cordano, A, Baertl, JM & Graham, GG (1964) Copper deficiency in infancy. Pediatrics 34, 324–326.CrossRefGoogle ScholarPubMed
Dorner, K, Dziadzka, S, Hohn, A, Sievers, E, Oldigs, HD, Schulz-Lell, G & Schaub, J (1989) Longitudinal manganese and copper balances in young infants and preterm infants fed on breast milk and adapted cow's milk formulas. Br J Nutr 61, 559–572.CrossRefGoogle ScholarPubMed
Drabkins, DL & Austin, JH (1932) Spectrophotometric constants for common hemoglobin derivatives in human, dog and rabbit blood. J Biol Chem 98, 719–733.Google Scholar
Friedewald, WT, Levy, RI & Frederickson, DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18, 499–502.CrossRefGoogle ScholarPubMed
Goyens, O, Brasseur, D & Cadranel, S (1985) Copper deficiency in infants with active celiac disease. J Pediatr Gastroenterol Nutr 4, 677–680.Google ScholarPubMed
Harvey, LJ, Majsak-Newman, G, Dainty, JR, Wharf, SG, Reid, MD, Beattie, JH & Fairweather-Tait, SJ (2002) Holmium as a fecal marker for copper absorption studies in adults. Clin Sci 102, 233–240.CrossRefGoogle ScholarPubMed
Henry, RJ, Chiamori, N, Jacobs, SL & Segalove, M (1960) Determination of ceruloplasmin oxidase in serum. Proc Soc Exp Biol Med 104, 620–624.CrossRefGoogle Scholar
Ishihara, N & Matsushiro, T (1986) Biliary and urinary excretion of metals in humans. Arch Environ Health 41, 324–330.CrossRefGoogle ScholarPubMed
Jones, DG & Suttle, NF (1981) Some effects of copper deficiency on leucocyte function in sheep and cattle. Res Vet Sci 31, 151–156.CrossRefGoogle ScholarPubMed
Linder, MC (1991) In The Biochemistry of Copper. New York, NY: Plenum Press.CrossRefGoogle Scholar
Linder, MC, Roboz, M & and the Los Alamos Medical Radioisotope Research Group (1986) Turnover and excretion of copper in rats as measured with 67Cu. Am J Physiol 251, E551–E555.Google ScholarPubMed
Owen, CA (1971) Metabolism of copper 67 by the copper-deficient rat. Am J Physiol 221, 1722–1727.CrossRefGoogle ScholarPubMed
Paglia, DE & Valentine, WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70, 158–169.Google ScholarPubMed
Reiser, S, Smith, JC Jr, Mertz, W, Holbrook, JT, Schofield, DJ & Powell, AS (1985) Indices of copper status in humans consuming a typical American diet containing either fructose or starch. Am J Clin Nutr 42, 242–251.CrossRefGoogle ScholarPubMed
Royal Society of Chemistry (1991) In McCance & Widdowson's The Composition of Foods, 5th ed. [Holland, B, Welch, AA, Unwin, ID, Buss, DH, Paul, AA and Southgate, DAT, editors]. London: Royal Society of Chemistry and Ministry of Agriculture, Fisheries and Food.Google Scholar
Scott, KC & Turnlund, JR (1994) Compartmental model of copper metabolism in adult men. J Nutr Biochem 5, 342–350.CrossRefGoogle Scholar
Turnlund, JR, Keyes, WR, Anderson, HL & Acord, LL (1989) Copper absorption and retention in young men at three levels of dietary copper using the stable isotope 65Cu. Am J Clin Nutr 49, 870–878.CrossRefGoogle ScholarPubMed
Turnlund, JR, Keyes, WR, Hudson, CA, Betschart, AA, Kretsch, MJ & Sauberlich, HE (1991) A stable isotope study of zinc, copper and iron absorption and retention by young women fed vitamin B-6 deficient diets. Am J Clin Nutr 54, 1059–1064.CrossRefGoogle ScholarPubMed
Turnlund, JR, Keyes, WR, Peiffer, GL & Scott, KC (1998) Copper absorption, excretion and retention by young men consuming low dietary copper determined by using the stable isotope 65Cu. Am J Clin Nutr 67, 1219–1225.CrossRefGoogle ScholarPubMed
Turnlund, JR, Wada, L, King, JC, Keyes, WR & Acord, LL (1988) Copper absorption in young men fed adequate and low zinc diets. Biol Trace Elem Res 17, 31–41.CrossRefGoogle ScholarPubMed
Williams, WJ, Beutler, E, Erslev, AJ & Rundles, RW (1977) In Hematology. New York, NY: McGraw-Hill.Google Scholar