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Absorption kinetics of dietary hydrolysis products in conscious pigs given diets with different amounts of fish protein

2. Individual amino acids

Published online by Cambridge University Press:  09 March 2007

A. Rérat ,
J. Jung  and
J. Kandé
A. Rérat
Affiliation:
Laboratoire de Physiologie de la Nutrition, INRA, Centre de Recherches de Jouy-en-Josas, 78350 Jouy-en-Josas, France
J. Jung
Affiliation:
Laboratoire de Physiologie de la Nutrition, INRA, Centre de Recherches de Jouy-en-Josas, 78350 Jouy-en-Josas, France
J. Kandé
Affiliation:
Centre de Recherches sur la Nutrition, CNRS, 9 Rue Hetzel, 92190 Meudon-Bellevue, France
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Abstract

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1. Concentrations of amino acids (AA) in blood obtained from arterial and portal permanent catheters were measured together with the portal hepatic blood flow-rate during a post-prandial period of 8 h, in nine conscious pigs (initial mean body-weight 49.3 (SEM 1.8) kg) receiving experimental meals (500, 600 or 1000 g) at 3–4 d intervals from 6–8 to 20 d after the surgical implantation of the catheters and electromagnetic flow probe. The semi-synthetic starch-based diets contained variable amounts of fish meal giving crude protein (nitrogen x 6.25; CP) concentrations (g/kg) of 80 (seven meals), 120 (seven meals), 160 (five meals) and 240 (three meals).

2. During the post-prandial period, variations in the individual blood AA concentrations were parallel to those of total amino-N (Rérat et al. 1988) to a greater or lesser extent according to the AA considered. Portal concentrations, which always exceeded arterial ones (except for glutamic acid and glutamine), increased quickly and, after a peak, returned slowly to reach initial values (small intake) or above (large intake) after 8 h.

3. Relations between amounts of each AA appearing during 8 h after the meal and amounts ingested were characterized by a highly significant linear regression (with the exceptions of glutamic acid and cystine). There were also close and significant relations between amounts of AA absorbed during the first 2 and 4 h after the meal and the amounts ingested.

4. For a mean intake of 90 g CP, aromatic AA showed the highest hourly absorption coefficients (about 010 /h), and sulphur-AA (0.07/h), lysine (007/h) and arginine (0.056/h) the lowest ones. Alanine was synthesized (amounts absorbed within 8 h exceeding those ingested) at the expense of glutamic acid (absorption coefficient 001/h).

5. For a given period of time, the AA abscrption coefficients decreased with increasing intake, but not in the same proportions for all AA, resulting in an enrichment (lysine, arginine, serine, proline) or depletion (branched- chain AA, histidine) of the absorbed mixture.

6. Some substances of the urea cycle were synthesized in rather large amounts in the gut wall (for a mean level of intake of 90 g CP: citrulline 2.41 g/8 h, ornithine 1.09 g/8 h). Blood glutamine was taken up by the gut wall in larger amounts (4.28 g/8 h).

Type
General Nutrition Papers
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 105 - 120
Copyright
Copyright © The Nutrition Society 1988

References

Bloxam, D. L. (1972) British Journal of Nutrition 21, 233–247.CrossRefGoogle Scholar
Boomgaardt, J. &, McDonald, B. E (1969) Canadian Journal of Physiology and Pharmacology 47, 392–395.CrossRefGoogle Scholar
Cuber, J. C. &, Laplace, J. P. (1979) Annales de Zootechnie 28, 173–184.CrossRefGoogle Scholar
Darcy, B., Laplace, J. P. &, Duke, P. H. (1982) Annales de Zootechnie 31, 279–300.CrossRefGoogle Scholar
Dawson, R. &, Holdsworth, E. S. (1962) British Journal of Nutrition 10, 13–25.CrossRefGoogle Scholar
Dent, C. E. &, Schilling., J. A. (1949) Biochemical Journal 44, 318–333.CrossRefGoogle Scholar
Denton, A. E. &, Elvehjem, C. A. (1954) Journaf of Biologicaf Chemistry 206, 449–454.CrossRefGoogle Scholar
Elwyn, D. H. (1970). In Mammalian Protein Metabolism, Vol. 4, pp. 523–557 [Munro, H. N., editor]. New York and London: Academic Press.CrossRefGoogle Scholar
Elwyn, D. H., Parikh, H. C. &, Shoemaker, W. C. (1968) American Journal of Physiology 215, 1260–1275.CrossRefGoogle Scholar
Henry, Y. &, Rérat, A. (1964) Annales de Biologie Animale, Biochimie, Biophysique 4, 263–271.CrossRefGoogle Scholar
Holmes, J. H. G., Bayley, H. S., Leadbeater, P. A. &, Horney, F. D. (1974) British Journal of Nutrition 32, 479–489.CrossRefGoogle Scholar
Krysciak, J., Ostrowski, H. &, Rys, R. (1966) Acta Biochemica Polonica 13, 229–235.Google Scholar
Laplace, J. P., Pons, O., Cuber, J. C., Kabori, C. &, Villiers, P. A. (1981) Annales de Zootechnie 30, 209–248.CrossRefGoogle Scholar
McLaughlan, J. M., Noel, F. J., Morrison, A. B. &, Campbell, J. A. (1963) Canadian Journal of Biochemistry and Physiology 41, 191–199.CrossRefGoogle Scholar
Moore, S., Spackmann, D. H. &, Stein, W. H. (1958) Analytical Chemistry 30, 1185–1190.CrossRefGoogle Scholar
Murer, H., Sigrist-Nelson, K. &, Hopfer, U. (1975) Journal of Biological Chemisrry 250, 7392–7396.CrossRefGoogle Scholar
Neame, K. D. &, Wiseman, G. (1957) Journal of Physiology 135, 442–450.CrossRefGoogle Scholar
Nordstrom, J. W., Windels, H. W., Typpo, J. T., Meade, R. J. &, Stockland, W. L. (1970) Journal of Aninid Science 31, 874–884.CrossRefGoogle Scholar
Ostrowski, H. (1969) Animal Production 11, 521–532.Google Scholar
Peraino, C. &, Harper, A. E. (1963) Journal of Nutrition 80, 270–278.CrossRefGoogle Scholar
Pion, R., Fauconneau, G. &, Rérat, A. (1963) Annales de Biologie Animale, Biochimie, Biophysique 3, 31–37.CrossRefGoogle Scholar
Pion, R., Fauconneau, G. &, Rérat, A. (1964) Annales de Biologie Aiiimale, Biochimie, Biophysique 4, 383–402.CrossRefGoogle Scholar
Pion, R. &, Rérat, A. (1967) Comptes Rendus Hebdomadaires des Séances de l' Académie des Sciences, Paris, 264D, 632–635.Google Scholar
Rérat, A. (1981) Bulletin de l' Académie Nationale de Médecine (Paris) 165, 1131–1137.Google Scholar
Rérat, A. (1982). In Digestive Physiology in the Pig. Colloques de l'INRA no. 12, pp. 63–85 [Laplace, J.P., Corring, T. and Rérat, A., editors]. Pans: Institut National de la Recherche Agronomique.Google Scholar
Rérat, A., Corring, T. &, Laplace, J. P. (1976). Protein Metabolism and Nutrition. European Association of Animal Production Publication no 16, pp. 97–138 [Cole, D.J. A., Boorman, K. N., Buttery, P. J., Lewis, D., Neale, R. J., Swan, H., editors]. London: Butterworths.Google Scholar
Rérat, A., Simoes Nunes, C., Vaissade, P. &, Roger, L. (1987) Reproduction Nutrition, Développement 27, 955–966.CrossRefGoogle Scholar
Rérat, A., Vaissade, P. &, Vaugelade, P. (1988) British Journal of Nutrition 60, 91–104.CrossRefGoogle Scholar
Rérat, A., Vaugelade, P. &, Villiers, P. A. (1980). In Current Concepts of Digestion and Absorption in the Pig, Technical Bulletin no. 3, pp. 177–216 [Low, A.G., Partridge, I.G., editors]. Reading/ Ayr: National Institute for Research in Dairying/Hannah Research Institute.Google Scholar
Richardson, L. R., Hale, F. &, Ritchey, S. J. (1965) Journal of Animal Science 24, 368–372.CrossRefGoogle Scholar
Typpo, J. T., Meade, R. J., Nordstrom, J. W. &, Stockland, W. L. (1970) Journal of Animal Science 31, 885–893.CrossRefGoogle Scholar
Waring, J. J. &, Bolton, W. (1967). 5th Colloquium on Amino Acid Analysis, Technicon Monograph no. 2 pp. 30–34. Domont, France: Technicon. Instruments Corp.Google Scholar
Weller, L. A., Margen, S. &, Calloway, D. H. (1969) American Journal of Clinical Nutrition 22, 1577–1583.CrossRefGoogle Scholar
Whitaker, T. R. &, Patrick, H. A. (1971). Bulletin of the Agricultural Experimental Station, West Virginia University no. 605T, pp. 1–27.Google Scholar
Windmueller, H. G. &, Spaeth, A. E. (1976) Archives of Biochemistry and Biophysics 175, 670–676.CrossRefGoogle Scholar
Windmueller, H. G. &, Spaeth, A. E. (1980) Journal of Biological Chemistry 255, 107–112.CrossRefGoogle Scholar
Zebrowska, T. (1981). In Nutrition in Health and Disease and International Development, pp. 909–919 [Harper, A.E. and Davis, G. K., editors]. New York: Alan Liss Inc.Google Scholar