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Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp.

Published online by Cambridge University Press:  06 July 2020

Tengku Athirrah Tengku-Mazuki ,
Kavilasni Subramaniam ,
Nur Nadhirah Zakaria ,
Peter Convey ,
Khalilah Abdul Khalil ,
Gillian Li Yin Lee ,
Azham Zulkharnain ,
Noor Azmi Shaharuddin  and
Siti Aqlima Ahmad Open the ORCID record for Siti Aqlima Ahmad [Opens in a new window]
Tengku Athirrah Tengku-Mazuki
Affiliation:
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400Serdang, Selangor, Malaysia
Kavilasni Subramaniam
Affiliation:
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400Serdang, Selangor, Malaysia
Nur Nadhirah Zakaria
Affiliation:
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400Serdang, Selangor, Malaysia
Peter Convey
Affiliation:
British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 0ET, UK
Khalilah Abdul Khalil
Affiliation:
Department of Biomolecular Sciences, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia
Gillian Li Yin Lee
Affiliation:
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400Serdang, Selangor, Malaysia
Azham Zulkharnain
Affiliation:
Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 11 307 Fukasaku, Minuma-ku, Saitama, 337-8570, Japan
Noor Azmi Shaharuddin
Affiliation:
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400Serdang, Selangor, Malaysia
Siti Aqlima Ahmad*
Affiliation:
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400Serdang, Selangor, Malaysia National Antarctic Research Centre, B303 Level 3, Block B, IPS Building, Universiti Malaya, 50603Kuala Lumpur, Malaysia
*
[email protected]
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Abstract

This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol.

Keywords

bioremediationone-factor-at-a-timeresponse surface methodology
Type
Biological Sciences
Information
Antarctic Science , Volume 32 , Issue 6 , December 2020 , pp. 486 - 495
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Copyright
Copyright © Antarctic Science Ltd 2020

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