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A microbiologically clean strategy for access to the Whillans Ice Stream subglacial environment

Published online by Cambridge University Press:  28 March 2013

John C. Priscu ,
Amanda M. Achberger ,
Joel E. Cahoon ,
Brent C. Christner ,
Robert L. Edwards ,
Warren L. Jones ,
Alexander B. Michaud ,
Matthew R. Siegfried ,
Mark L. Skidmore  and
Robert H. Spigel
...Show all authors
John C. Priscu*
Affiliation:
Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT 59717, USA
Amanda M. Achberger
Affiliation:
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
Joel E. Cahoon
Affiliation:
Department of Civil Engineering, Montana State University, Bozeman, MT 59717, USA
Brent C. Christner
Affiliation:
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
Robert L. Edwards
Affiliation:
Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT 59717, USA
Warren L. Jones
Affiliation:
Department of Civil Engineering, Montana State University, Bozeman, MT 59717, USA
Alexander B. Michaud
Affiliation:
Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT 59717, USA
Matthew R. Siegfried
Affiliation:
Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093, USA
Mark L. Skidmore
Affiliation:
Department of Earth Science, Montana State University, Bozeman, MT 59717, USA
Robert H. Spigel
Affiliation:
National Institute of Water and Atmospheric Research Ltd, Box 8602, Christchurch, New Zealand
Gregg W. Switzer
Affiliation:
Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT 59717, USA
Slawek Tulaczyk
Affiliation:
Department of Earth and Planetary Sciences, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
Trista J. Vick-Majors
Affiliation:
Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT 59717, USA
*
[email protected]
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Abstract

The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project will test the overarching hypothesis that an active hydrological system exists beneath a West Antarctic ice stream that exerts a major control on ice dynamics, and the metabolic and phylogenetic diversity of the microbial community in subglacial water and sediment. WISSARD will explore Subglacial Lake Whillans (SLW, unofficial name) and its outflow toward the grounding line where it is thought to enter the Ross Ice Shelf seawater cavity. Introducing microbial contamination to the subglacial environment during drilling operations could compromise environmental stewardship and the science objectives of the project, consequently we developed a set of tools and procedures to directly address these issues. WISSARD hot water drilling efforts will include a custom water treatment system designed to remove micron and sub-micron sized particles (biotic and abiotic), irradiate the drilling water with germicidal ultraviolet (UV) radiation, and pasteurize the water to reduce the viability of persisting microbial contamination. Our clean access protocols also include methods to reduce microbial contamination on the surfaces of cables/hoses and down-borehole equipment using germicidal UV exposure and chemical disinfection. This paper presents experimental data showing that our protocols will meet expectations established by international agreement between participating Antarctic nations.

Keywords

clean accessenvironmental stewardshiphot water drillingsubglacial aquatic environments
Type
Biological Sciences
Information
Antarctic Science , Volume 25 , Issue 5 , October 2013 , pp. 637 - 647
Copyright
Copyright © Antarctic Science Ltd 2013 

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References

Bell, R.E., Studinger, M., Tikku, A.A., Clarke, G.K.C., Gutner, M.M.Meertens, C. 2002. Origin and fate of Lake Vostok water frozen to the base of the East Antarctic ice sheet. Nature, 416, 307–310.CrossRefGoogle Scholar
Carter, S.P.Fricker, H.A. 2012. The supply of subglacial meltwater to the grounding line of the Siple Coast, West Antarctica. Annals of Glaciology, 53, 267–280.CrossRefGoogle Scholar
Carter, S.P., Fricker, H.A., Blankenship, D.D., Young, D.A., Johnson, J.V., Price, S.F.Lipscomb, V. 2011. Modeling five years of subglacial lake activity in the MacAyeal Ice Stream catchment through assimilation of ICESat laser altimetry. Journal of Glaciology, 57, 1098–1112.CrossRefGoogle Scholar
Christianson, K., Jacobel, R.W., Horgan, H.J., Anandakrishnan, S.Alley, R.B. 2012. Subglacial lake Whillans: ice-penetrating radar and GPS observations of a shallow active reservoir beneath a West Antarctic ice stream. Earth and Planetary Science Letters, 331, 237–245.CrossRefGoogle Scholar
Christner, B.C., Skidmore, M.L., Priscu, J.C., Tranter, M.Foreman, C.M. 2008. Bacteria in subglacial environments. In Margesin, R., Schinner, F., Marx, J.-C.&Gerday, C.,eds. Psychrophiles: from biodiversity to biotechology. New York: Springer, 51–71.CrossRefGoogle Scholar
Christner, B.C., Royston-Bishop, G., Foreman, C.M., Arnold, B.R., Tranter, M., Welch, K.A., Lyons, W.B., Tsapin, A.I.Priscu, J.C. 2006. Limnological conditions in Subglacial Lake Vostok, Antarctica. Limnology and Oceanography, 51, 2485–2501.CrossRefGoogle Scholar
Dankwerts, P.V. 1953. Continuous flow systems. Distribution of residence times. Chemical Engineering Science, 2, 1–13.CrossRefGoogle Scholar
Fricker, H.A.Scambos, T. 2009. Connected subglacial lake activity on lower Mercer and Whillans ice streams, West Antarctica, 2003–2008. Journal of Glaciology, 55, 303–315.CrossRefGoogle Scholar
Fricker, H.A., Scambos, T.A., Bindschadler, R.A.Padman, L. 2007. An active subglacial water system in West Antarctica mapped from space. Science, 315, 1544–1548.CrossRefGoogle ScholarPubMed
Fricker, H.A., Powell, R., Priscu, J.C., Tulaczyk, S., Anandakrishnan, S., Christner, B., Holland, D., Horgan, H., Mikucki, J., Mitchell, A., Scherer, R.Severinghaus, J. 2011. Siple Coast subglacial aquatic environments: the Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project. In Siegert, M.J., Kennicutt II, M.C., Bindschadler, R.A.,eds. Antarctic subglacial aquatic environments. American Geophysical Union Geophysical Monograph, 192, 199–219.CrossRefGoogle Scholar
Gray, L., Joughin, I., Tulaczyk, S., Spikes, V.B., Bindschadler, R.Jezek, K. 2005. Evidence for subglacial water transport in the West Antarctic Ice Sheet through three-dimensional satellite radar interferometry. Geophysical Research Letters, 10.1029/2004GL021387.CrossRefGoogle Scholar
Horgan, H.J., Anandakrishnan, S., Jacobel, R.W., Christianson, K., Alley, R.B., Heeszel, D.S., Picotti, S.Walter, J.I. 2012. Subglacial Lake Whillans: seismic observations of a shallow active reservoir beneath a West Antarctic ice stream. Earth and Planetary Science Letters, 331-332, 201–209.CrossRefGoogle Scholar
Karl, D.M. 1980. Cellular nucleotide measurements and applications in microbial ecology. Microbiological Reviews, 44, 739–796.CrossRefGoogle Scholar
Karl, D.M., Bird, D.F., Bjorkman, K., Houlihan, T., Shackelford, R.Tupas, L. 1999. Microorganisms in the accreted ice of Lake Vostok, Antarctica. Science, 286, 2144–2147.CrossRefGoogle ScholarPubMed
Lanoil, B., Skidmore, M., Priscu, J.C., Han, S., Foo, W., Vogel, S.W., Tulaczyk, S.Engelhardt, H. 2009. Bacteria beneath the West Antarctic Ice Sheet. Environmental Microbiology, 11, 609–615.CrossRefGoogle ScholarPubMed
Lisle, J.T.Priscu, J.C. 2004. The occurrence of lysogenic bacteria and microbial aggregates in the lakes of the McMurdo Dry Valleys, Antarctica. Microbial Ecology, 47, 427–439.CrossRefGoogle ScholarPubMed
Lukin, V.Bulat, S. 2011. Vostok Subglacial Lake: details of Russian plans/activities for drilling and sampling. In Siegert, M.J., Kennicutt II, M.C., Bindschadler, R.A.,eds. Antarctic subglacial aquatic environments. American Geophysical Union Geophysical Monograph, 192, 187–197.CrossRefGoogle Scholar
NRC (National Research Council). 2007. Exploration of Antarctic subglacial aquatic environments: environmental and scientific stewardship. Washington, DC: National Academies Press, 166 pp.Google Scholar
Priscu, J.C. 2002. Commentary: subglacial lakes have changed our view of Antarctica. Antarctic Science, 14, 291.Google Scholar
Priscu, J.C.Christner, B. 2004. Earth's icy biosphere. In Bull, A.T., ed. Microbial diversity and prospecting. Washington, DC: ASM Press, 130–145.Google Scholar
Priscu, J.C., Powell, R.D.Tulaczyk, S. 2010. Probing subglacial environments under the Whillans Ice Stream. EOS Transactions, 91, 253–254.CrossRefGoogle Scholar
Priscu, J.C., Christner, B.C., Foreman, C.M.Royston-Bishop, G. 2007. Biological material in ice cores. In Elias, S.A., ed. Encyclopedia of Quaternary sciences. Vol. 2. Amsterdam: Elsevier, 1156–1166.CrossRefGoogle Scholar
Priscu, J.C., Tulaczyk, S., Studinger, M., Kennicutt II, M.C., Christner, B.C.Foreman, C.M. 2008. Antarctic subglacial water: origin, evolution and ecology. In Vincent, W.&Laybourn-Parry, J.,eds. Polar lakes and rivers. Oxford: Oxford University Press, 119–135.CrossRefGoogle Scholar
Priscu, J.C., Kennicutt II, M.C., Bell, R.E., Bulat, S.A., Ellis-Evans, C., Lukin, V.V., Petit, J-R., Powell, R.D., Siegert, M.J.Tabacco, I. 2003. An international plan for Antarctic subglacial lake exploration. Polar Geography, 27, 69–83.CrossRefGoogle Scholar
Priscu, J.C., Kennicutt II, M.C., Bell, R.E., Bulat, S.A., Ellis-Evans, C., Lukin, V.V., Petit, J-R., Powell, R.D., Siegert, M.J.Tabacco, I. 2005. Exploring subglacial Antarctic lake environments. EOS Transactions, 86, 193–200.CrossRefGoogle Scholar
Priscu, J.C., Adams, E.E., Lyons, W.B., Voytek, M.A., Mogk, D.W., Brown, R.L., McKay, C.P., Takacs, C.D., Welch, K.A., Wolf, C.F., Kirshtein, J.D.Avci, R. 1999. Geomicrobiology of subglacial ice above Lake Vostok, Antarctica. Science, 286, 2141–2144.CrossRefGoogle ScholarPubMed
Rignot, E., Mouginot, J.Scheuchl, B. 2011. Ice flow of the Antarctic Ice Sheet. Science, 333, 1427–1430.CrossRefGoogle ScholarPubMed
Roslev, P.King, G.M. 1993. Application of a tetrazolium salt with a water-soluble formazan as an indicator of viability in respiring bacteria. Applied and Environmental Microbiology, 59, 2891–2896.CrossRefGoogle ScholarPubMed
Ross, N., Siegert, M.J., Rivera, A., Bentley, M., Blake, D., Capper, L., Clarke, R., Cockell, C., Corr, H., Harris, W., Hill, C., Hindmarsh, R., Hodgson, D., King, E., Lamb, H., Maher, B., Makinson, K., Mowlem, M., Parnell, J., Pearce, D., Priscu, J.C., Smith, A., Tait, A., Tranter, M., Wadham, J., Whalley, B.Woodward, J. 2011. Subglacial Lake Ellsworth, West Antarctica: its history, recent field campaigns and plans for its exploration. In Siegert, M.J., Kennicutt II, M.C., Bindschadler, R.A.,eds. Antarctic subglacial aquatic environments. American Geophysical Union Geophysical Monograph, 192, 221–233.CrossRefGoogle Scholar
Scambos, T., Haran, T., Fahnestock, M., Painter, T.Bohlander, J. 2007. MODIS-based Mosaic of Antarctica (MOA) data sets: continent-wide surface morphology and snow grain size. Remote Sensing of Environment, 111, 242–257.CrossRefGoogle Scholar
Shabtaie, S., Whillans, I.M.Bentley, C.R. 1987. The morphology of Ice Streams A, B, and C, West Antarctica, and their environs. Journal of Geophysical Research, 92, 8865–8883.CrossRefGoogle Scholar
Siegert, M.J., Carter, S., Tabacco, I., Popov, S.Blankenship, D.D. 2005. A revised inventory of Antarctic subglacial lakes. Antarctic Science, 17, 453–460.CrossRefGoogle Scholar
Siegert, M.J., Clarke, R.J., Mowlem, M., Ross, N., Hill, C.S., Tait, A., Hodgson, D., Parnell, J., Tranter, M., Pearce, D., Bentley, M.J., Cockell, C., Tsaloglou, M.N., Smith, A., Woodward, J., Brito, M.P.Waugh, E. 2012. Clean access, measurement, and sampling of Ellsworth Subglacial Lake: a method for exploring deep Antarctic subglacial lake environment. Reviews in Geophysics, 10.1029/2011RG000361.CrossRefGoogle Scholar
Smith, B.E., Fricker, H.A., Joughin, I.R.Tulaczyk, S. 2009. An inventory of active subglacial lakes in Antarctica detected by ICESat (2003–2008). Journal of Glaciology, 55, 573–595.CrossRefGoogle Scholar
Vanoni, V.A. 1975. Sedimentation engineering. ASCE Manual 54. Prepared by ASCE Task Committee for the preparation of the manual on sedimentation of the Sedimentation Committee of the Hydraulics Division. Reston, VA: American Society for Civil Engineering, 418 pp.Google Scholar
Wingham, D.J., Siegert, M.J., Shepherd, A.Muir, A.S. 2006. Rapid discharge connects Antarctic subglacial lakes. Nature, 440, 1033–1036.CrossRefGoogle ScholarPubMed