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Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica

Published online by Cambridge University Press:  16 December 2021

Viktoriia Holovan Open the ORCID record for Viktoriia Holovan [Opens in a new window] ,
Olena Andriichuk ,
Irena Budzanivska ,
Pavlina Zelena ,
Tetiana Kondratiuk  and
Oleksiy Shevchenko
Viktoriia Holovan*
Affiliation:
Educational and Scientific Center ‘Institute of Biology and Medicine’, Taras Shevchenko National University of Kyiv, Volodymyrska str., 64/13, Kyiv 01601, Ukraine
Olena Andriichuk
Affiliation:
Educational and Scientific Center ‘Institute of Biology and Medicine’, Taras Shevchenko National University of Kyiv, Volodymyrska str., 64/13, Kyiv 01601, Ukraine
Irena Budzanivska
Affiliation:
Educational and Scientific Center ‘Institute of Biology and Medicine’, Taras Shevchenko National University of Kyiv, Volodymyrska str., 64/13, Kyiv 01601, Ukraine
Pavlina Zelena
Affiliation:
Educational and Scientific Center ‘Institute of Biology and Medicine’, Taras Shevchenko National University of Kyiv, Volodymyrska str., 64/13, Kyiv 01601, Ukraine
Tetiana Kondratiuk
Affiliation:
Educational and Scientific Center ‘Institute of Biology and Medicine’, Taras Shevchenko National University of Kyiv, Volodymyrska str., 64/13, Kyiv 01601, Ukraine
Oleksiy Shevchenko
Affiliation:
Educational and Scientific Center ‘Institute of Biology and Medicine’, Taras Shevchenko National University of Kyiv, Volodymyrska str., 64/13, Kyiv 01601, Ukraine
*
[email protected]
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Abstract

Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU 540) were recovered and characterized. Isolated bacteria belonged to Pseudomonas genus (Pseudomonas sp., Pseudomonas fluorescens, Pseudomonas putida) with optimal cultivation temperatures of 16–28°C. These bacteria and previously described Bacillus subtilis FCKU 537 were used for analysing virus-host interactions. Six lytic phages were isolated and named P. fluorescens Antarctic virus 1 (PFAV1), P. fluorescens Antarctic virus 2 (PFAV2), P. fluorescens Antarctic virus 3 (PFAV3), P. putida Antarctic virus 4 (PPAV4), Pseudomonas sp. Antarctic virus 5 (PSAV5) and B. subtilis Antarctic virus 6 (BSAV6) in relation to their natural hosts. According to electron microscopy data, these phages belonged to Caudovirales order. Cross-inoculation demonstrated high specificity of all Antarctic phages, which infected only their initial hosts at moderate temperatures. PFAV2 and PFAV3 phages also infected laboratory Pseudomonas savastanoi and P. fluorescens isolates. This paper adds new data on the occurrence and diversity of viruses and their respective bacterial hosts in soil biotopes of Antarctica.

Keywords

bacteriaelectron microscopysoilvirus particles
Type
Biological Sciences
Information
Antarctic Science , Volume 34 , Issue 2 , April 2022 , pp. 120 - 136
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Copyright
Copyright © Antarctic Science Ltd 2021

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