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Thermophilic bacteria present in a sample from Fumarole Bay, Deception Island

Published online by Cambridge University Press:  27 June 2011

Patricio A. Muñoz ,
Patricio A. Flores ,
Freddy A. Boehmwald  and
Jenny M. Blamey
Patricio A. Muñoz*
Affiliation:
Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Santiago, Chile Doctorado en Biotecnología, Universidad de Santiago de Chile, Avenida Libertador Bernardo O'Higgins 3363, Santiago, Chile
Patricio A. Flores
Affiliation:
Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Santiago, Chile Doctorado en Biotecnología, Universidad de Santiago de Chile, Avenida Libertador Bernardo O'Higgins 3363, Santiago, Chile
Freddy A. Boehmwald
Affiliation:
Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Santiago, Chile
Jenny M. Blamey
Affiliation:
Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Santiago, Chile
*
[email protected]
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Abstract

Deception Island, an active stratovolcano located in the South Shetland Islands, Antarctica, provides excellent conditions for the thermophilic bacteria growth because of high ground temperatures in specific areas, such as Fumarole Bay where the temperatures are above the mesophilic range. Denaturing Gradient Gel Electrophoresis (DGGE) was used with the 16S ribosomal gene to analyse cultures of thermophilic bacteria from a soil sample taken from Fumarole Bay. Nine bands were sequenced and analysed from DGGE and they indicated the presence of bacteria from the genera Geobacillus, Bacillus, Brevibacillus, Thermus and uncultured sulphate reducing bacteria. Some of which have been reported in other Antarctic geothermal sites. Geobacillus, Bacillus and Brevibacillus genera were successfully cultivated in an enriched medium. A pure culture of one thermophilic Geobacillus bacterium was obtained closely related to Geobacillus jurassicus.

Keywords

AntarcticaDGGESouth Shetland Islandsthermophiles
Type
Biological Sciences
Information
Antarctic Science , Volume 23 , Issue 6 , 22 November 2011 , pp. 549 - 555
Copyright
Copyright © Antarctic Science Ltd 2011

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