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Carotenoids and pH of the culture medium play an important role in displaying metal stress in batch and semi-continuous cultures of Anabaena doliolum

Published online by Cambridge University Press:  27 May 2009

Bhumi Nath Tripathi ,
Rohini Kasana ,
Vijeta Singh ,
Indu Bhatt ,
Ashutosh Singh ,
Vinay Sharma  and
Jai Prakash Gaur
Bhumi Nath Tripathi*
Affiliation:
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India
Rohini Kasana
Affiliation:
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India
Vijeta Singh
Affiliation:
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India
Indu Bhatt
Affiliation:
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India
Ashutosh Singh
Affiliation:
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India
Vinay Sharma
Affiliation:
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India
Jai Prakash Gaur
Affiliation:
Department of Botany, Banaras Hindu University, Varanasi, 221005, India
*
[email protected]
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Abstract

We analysed the responses of Anabaena doliolum to elevated levels of copper and zinc in batch and semi-continuous cultures. Approximately 10, 4 and 8 and 5-times greater inhibition in final yield of A. doliolum occurred at 1, 2 μM Cu and 2.5 and 5 μM of Zn, respectively, in semi-continuous culture in comparison to batch culture. Protein, chlorophyl a and carotenoid contents of A. doliolum showed significantly (P < 0.05) higher inhibition by test metals in semi-continuous culture than in batch culture. The greater sensitivity of different parameters of the test organism was related to the high metal content of the cells grown in semi-continuous system. Moreover, enhancement of pH of the culture suspension in batch culture showed a negative relationship with metal accumulation, and therefore with toxicity. This was due to decrease in free ionic concentrations of test metals. Carotenoids acted as a metal detoxifying agent by minimizing metal-induced inhibition in batch culture as was evident from its negative relationship with metal toxicity.

Keywords

Anabaena doliolumbatch culturecarotenoidscopperpHsemi-continuous culturezinc
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 45 , Issue 2 , 2009 , pp. 119 - 125
Copyright
© EDP Sciences, 2009

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