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Transcriptional regulation of acetyl-CoA carboxylase α isoforms in dairy ewes during conjugated linoleic acid induced milk fat depression

Published online by Cambridge University Press:  26 April 2016

E. Ticiani ,
M. Urio ,
R. Ferreira ,
K. J. Harvatine  and
D. E. De Oliveira
E. Ticiani
Affiliation:
Department of Animal Production, Santa Catarina State University, Lages, Santa Catarina, 88520-000, Brazil
M. Urio
Affiliation:
Department of Animal Production, Santa Catarina State University, Lages, Santa Catarina, 88520-000, Brazil
R. Ferreira
Affiliation:
Department of Animal Science, Santa Catarina State University, Chapecó, Santa Catarina, 89815-630, Brazil
K. J. Harvatine
Affiliation:
Department of Animal Science, Penn State University, University Park, PA 16802, USA
D. E. De Oliveira*
Affiliation:
Department of Animal Production, Santa Catarina State University, Lages, Santa Catarina, 88520-000, Brazil
*
†E-mail: [email protected]; [email protected]
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Abstract

Feeding trans-10, cis-12 CLA to lactating ewes reduces milk fat by down-regulating expression of enzymes involved in lipid synthesis in the mammary gland and increases adipose tissue lipogenesis. Acetyl-CoA carboxylase α (ACC-α) is a key regulated enzyme in de novo fatty acid synthesis and is decreased by CLA. In the ovine, the ACC-α gene is expressed from three tissue-specific promoters (PI, PII and PIII). This study evaluated promoter-specific ACC-α expression in mammary and adipose tissue of lactating cross-bred Lacaune/Texel ewes during milk fat depression induced by rumen-unprotected trans-10, cis-12 CLA supplement. In all, 12 ewes arranged in a completely randomized design were fed during early, mid and late lactation one of the following treatments for 14 days: Control (forage+0.9 kg of concentrate on a dry matter basis) and CLA (forage+0.9 kg of concentrate+27 g/day of CLA (29.9% trans-10, cis-12)). Mammary gland and adipose tissue biopsies were taken on day 14 for gene expression analysis by real-time PCR. Milk fat yield and concentration were reduced with CLA supplementation by 27%, 21% and 35% and 28%, 26% and 42% during early, mid and late lactation, respectively. Overall, our results suggest that trans-10, cis-12 CLA down-regulates mammary ACC-α gene expression by decreasing expression from PII and PIII in mammary gland and up-regulates adipose ACC-α gene expression by increasing expression from PI.

Keywords

gene expressionlactating ewemilk fat depression
Type
Research Article
Information
animal , Volume 10 , Issue 10 , October 2016 , pp. 1677 - 1683
Copyright
© The Animal Consortium 2016 

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