Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-24T16:25:08.919Z Has data issue: false hasContentIssue false
  • English
  • Français

Six placenta permeability-related genes: molecular characterization and expression analysis in pigs

Published online by Cambridge University Press:  01 March 2009

S.-P. Wu ,
X.-W. Xu ,
C.-C. Li ,
Y. Mei  and
S.-H. Zhao
S.-P. Wu
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China
X.-W. Xu
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China
C.-C. Li
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China
Y. Mei
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China
S.-H. Zhao*
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China
*
E-mail: [email protected]
Get access

Abstract

The nutrient transportation ability of placenta depends on placental size, vascular density and permeability. Regulation of angiogenesis in the placenta is critical for successful gestation. Placenta vascularity exhibits disparity in different gestation stages and different pig breeds. To investigate the expression of genes related to permeability in the porcine placenta of different gestation stages and breeds, molecular cloning and gene expression analysis of six porcine genes, vascular endothelial growth factor (VEGF), VEGF-R1, VEGF-R2, endothelial nitric oxide synthase (eNOS), vascular endothelial cadherin (CDH5) and β-arrestin2 (Arrb2), were performed in this study. The results demonstrated that from gestation day 33 to day 90, Landrace exhibited significant increase (P < 0.05) in placental VEGF and Arrb2 mRNA expression. Moreover, expression levels of VEGF, VEGF-R1, VEGF-R2 and eNOS mRNA were higher (P < 0.01) in the placenta of Erhualian than those in Landrace on day 90 of gestation. In contrast, CDH5 placental mRNA expression level exhibited significant decrease (P < 0.05) from day 33 to day 90 gestation in Landrace. Erhualian placental CDH5 and Arrb2 expression levels were significantly lower (P < 0.01) than those in Landrace conceptuses on day 90 of gestation. Our study offered new data on the expression of genes in VEGF signal transduction pathway in porcine placenta.

Keywords

pigplacentaexpressionpermeability
Type
Full Paper
Information
animal , Volume 3 , Issue 3 , March 2009 , pp. 408 - 414
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bates, DO, Harper, SJ 2002. Regulation of vascular permeability by vascular endothelial growth factors. Vascular Pharmacology 39, 225–237.CrossRefGoogle ScholarPubMed
Bates, DO, Lodwick, D, Williams, B 1999. Vascular endothelial growth factor and microvascular permeability. Microcirculation 6, 83–96.CrossRefGoogle ScholarPubMed
Biensen, NJ, Wilson, ME, Ford, SP 1998. The impact of either a Meishan or Yorkshire uterus on Meishan or Yorkshire fetal and placental development to days 70, 90, and 110 of gestation. Journal of Animal Science 76, 2169–2176.CrossRefGoogle ScholarPubMed
Feng, Z, Tang, ZL, Li, K, Liu, B, Yu, M, Zhao, SH 2007. Molecular characterization of the BTG2 and BTG3 genes in fetal muscle development of pigs. Gene 403, 170–177.CrossRefGoogle ScholarPubMed
Ford, SP, Youngs, CR 1993. Early embryonic development in prolific Meishan pigs. Journal of Reproduction and Fertility. Supplement 48, 271–278.Google ScholarPubMed
Gavard, J, Gutkind, JS 2006. VEGF controls endothelial-cell permeability by promoting the β-arrestin-dependent endocytosis of VE-cadherin. Nature Cell Biology 8, 1223–1234.CrossRefGoogle ScholarPubMed
Goodman, OB Jr, Krupnick, JG, Santini, F, Gurevich, VV, Penn, RB, Gagnon, AW, Keen, JH, Benovic, JL 1996. Beta-arrestin acts as a clathrin adaptor in endocytosis of the beta2-adrenergic receptor. Nature 383, 447–450.CrossRefGoogle ScholarPubMed
Hatakeyama, T, Pappas, PJ, Hobson, RW II, Boric, MP, Sessa, WC, Durán, WN 2006. Endothelial nitric oxide synthase regulates microvascular hyperpermeability in vivo. The Journal of Physiology 574, 275–281.CrossRefGoogle ScholarPubMed
Huber, P, Dalmon, J, Engiles, J, Breviario, F, Gory, S, Siracusa, LD, Buchberg, AM, Dejana, E 1996. Genomic structure and chromosomal mapping of the mouse VE-cadherin gene (CDH5). Genomics 32, 21–28.CrossRefGoogle ScholarPubMed
Kohout, TA, Lefkowitz, RJ 2003. Regulation of G protein-coupled receptor kinases and arrestins during receptor desensitization. Molecular Pharmacology 63, 9–18.CrossRefGoogle ScholarPubMed
Matthews, W, Jordan, CT, Gavin, M, Jenkins, NA, Copeland, NG, Lemischka, IR 1991. A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit. Proceedings of the National Academy of Sciences of the United States of America 88, 9026–9030.CrossRefGoogle ScholarPubMed
Michel, CC 1984. Fluid movements through capillary walls. In Handbook of physiology. The cardiovascular system. Microcirculation (ed. Michel CC and Renkin EM), pp. 375–409. American Physiological Society, Bethesda, USA.Google Scholar
Murohara, T, Horowitz, JR, Silver, M, Tsurumi, Y, Chen, D, Sullivan, A, Isner, JM 1998. Vascular endothelial growth factor/vascular permeability factor enhances vascular permeability via nitric oxide and prostacyclin. Circulation 97, 99–107.CrossRefGoogle ScholarPubMed
Olsson, AK, Dimberg, A, Kreuger, J, Claesson-Welsh, L 2006. VEGF receptor signaling-in control of vascular function. Nature Reviews. Molecular Cell Biology 7, 359–371.CrossRefGoogle Scholar
Shen, BQ, Lee, DY, Zioncheck, TF 1999. Vascular endothelial growth factor governs endothelial nitric-oxide synthase expression via a KDR/Flk-1 receptor and a protein kinase C signaling pathway. The Journal of Biological Chemistry 274, 33057–33063.CrossRefGoogle Scholar
Shibuya, M, Yamaguchi, S, Yamane, A, Ikeda, T, Tojo, A, Matsushime, H, Sato, M 1990. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family. Oncogene 5, 519–524.Google Scholar
Taylor, AE, Granger, DN 1984. Exchange of macromolecules across the microcirculation. In Handbook of physiology. The cardiovascular system. Microcirculation (ed. Michel CC and Renkin EM), pp. 467–520. American Physiological Society, Bethesda, USA.Google Scholar
Vallet, JL, Freking, BA 2007. Differences in placental structure during gestation associated with large and small pig fetuses. Journal of Animal Science 85, 3267–3275.CrossRefGoogle ScholarPubMed
Vonnahme, KA, Ford, SP 2004. Differential expression of the vascular endothelial growth factor-receptor system in the gravid uterus of Yorkshire and Meishan pigs. Biology of Reproduction 71, 163–169.CrossRefGoogle ScholarPubMed
Vonnahme, KA, Wilson, ME, Ford, SP 2001. Relationship between placental vascular endothelial growth factor expression and placental/endometrial vascularity in the pig. Biology of Reproduction 64, 1821–1825.CrossRefGoogle ScholarPubMed
Wang, Y, Pennock, SD, Chen, X, Kazlauskas, A, Wang, Z 2004. Platelet-derived growth factor receptor-mediated signal transduction from endosomes. The Journal of Biological Chemistry 279, 8038–8046.CrossRefGoogle ScholarPubMed
Wilson, ME, Biensen, NJ, Youngs, CR, Ford, SP 1998. Development of Meishan and Yorkshire littermate conceptuses in either a Meishan or Yorkshire uterine environment to day 90 of gestation and to term. Biology of Reproduction 58, 905–910.CrossRefGoogle ScholarPubMed
Wu, HM, Huang, Q, Yuan, Y, Granger, HJ 1996. VEGF induces NO-dependent hyperpermeability in coronary venules. The American Journal of Physiology 271, H2735–H2739.Google ScholarPubMed
Youngs, CR, Ford, SP, McGinnis, LK, Anderson, LH 1993. Investigations into the control of litter size in swine: I. Comparative studies on in vitro development of Meishan and Yorkshire preimplantation embryos. Journal of Animal Science 71, 1561–1565.CrossRefGoogle ScholarPubMed
Zanetta, L, Corada, M, Grazia Lampugnani, M, Zanetti, A, Breviario, F, Moons, L, Carmeliet, P, Pepper, MS, Dejana, E 2005. Downregulation of vascular endothelial-cadherin expression is associated with an increase in vascular tumor growth and hemorrhagic complications. Thrombosis and Haemostasis 93, 1041–1046.CrossRefGoogle ScholarPubMed