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Rabbit dietary supplementation with pale purple coneflower. 1. Effects on the reproductive performance and immune parameters of does

Published online by Cambridge University Press:  14 January 2016

S. Dabbou ,
L. Rotolo ,
A. Kovitvadhi ,
S. Bergagna ,
D. Dezzutto ,
R. Barbero ,
P. Rubiolo ,
A. Schiavone ,
M. De Marco  and
A. N. Helal
...Show all authors
S. Dabbou*
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy Laboratory of Bioresources, Integrative Biology and Valorisation, Higher Institute of Biotechnology of Monastir, av. Tahar Hadded, BP 74, 5000 Monastir, Tunisia
L. Rotolo
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy
A. Kovitvadhi
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy
S. Bergagna
Affiliation:
Veterinary Medical Research Institute for Piemonte, Liguria and the Valle D’Aosta, via Bologna 148, 10154 Torino, Italy
D. Dezzutto
Affiliation:
Veterinary Medical Research Institute for Piemonte, Liguria and the Valle D’Aosta, via Bologna 148, 10154 Torino, Italy
R. Barbero
Affiliation:
Veterinary Medical Research Institute for Piemonte, Liguria and the Valle D’Aosta, via Bologna 148, 10154 Torino, Italy
P. Rubiolo
Affiliation:
Department of Drug Science and Technology, University of Turin, Via P. Giuria 9, 10125 Torino, Italy
A. Schiavone
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy
M. De Marco
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy
A. N. Helal
Affiliation:
Laboratory of Bioresources, Integrative Biology and Valorisation, Higher Institute of Biotechnology of Monastir, av. Tahar Hadded, BP 74, 5000 Monastir, Tunisia
I. Zoccarato
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy
L. Gasco
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy Institute of Science of Food Production, National Research Council, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy
*
†E-mail: [email protected]
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Abstract

Echinacea pallida (EPAL), also known as pale purple coneflower, is a herbaceous flowering plant with immune-enhancement and antioxidative properties. The effect of EPAL on the reproductive performance, serum biochemistry and haematological parameters of rabbit does has been studied here. A total of 100, 21-week-old Grimaud rabbit does, were randomly assigned to two groups. One group was fed a basal diet supplemented with 3 g EPAL/kg diet (Echinacea group, E), while the other was fed the basal diet without the supplementation (control group, C). The reproductive performance of the does was not affected by the treatment (P>0.05). The haematological parameters of pregnant rabbits showed that there was no interaction between gestation day and treatment. The EPAL supplementation induced a reduction (−47.3%) in the basophil cell rate (0.55% and 0.29%, for the control and treatment groups, respectively; P=0.049). The gestation day significantly affected most of the haematological parameters (P<0.05). The white blood cell counts declined progressively after day 14. The mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, red cell distribution width, mean platelet volume and eosinophils increased steadily throughout the study, and reached a maximum value on day 28. The red blood cells, haemoglobin, haematocrit, mean corpuscular volume and neutrophils increased slightly up to day 14, and then subsequently decreased progressively until day 28. The lymphocytes and platelet distribution width decreased until day 14, and then increased to a maximum value on day 28. No significant effect of gestation day or treatment was observed on the blood serum chemistry. As far as the immune parameters are concerned, no significant differences were observed between groups, while a significant effect of gestation day was observed for lysozymes (6.02 v. 7.99 v. 1.91; for 0, 14 and 28 days, respectively; P=0.014). In conclusion, a lack of effect of EPAL has been observed. In fact, no impacts of EPAL have been observed on the reproductive or haematological parameters of the does. The effects of dietary supplementation with EPAL on the performances, bacterial community, blood parameters and immunity in growing rabbits are reported in the second part of this study.

Keywords

pale purple coneflowerEchinacea pallidarabbit doeshaematologyimmunity
Type
Research Article
Information
animal , Volume 10 , Issue 7 , July 2016 , pp. 1101 - 1109
Copyright
© The Animal Consortium 2016 

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References

Ahmed, HS, Kamel, KI, El-Sabeiy, ME and Zeitouny, MH 2008. Effect of Echinacea extract supplementation on growth performance and hemo-biochemical traits of growing rabbits. Egypt Poultry Science 28, 1165–1180.Google Scholar
AOAC 2000. Official methods of analysis, 17th edition. Association of official analytical chemists, Gaithersburg, Maryland, USA.Google Scholar
Arafa, NMS, Salem, SM and Farid, OAHA 2010. Influence of Echinacea extract pre- or postnatal supplementation on immune and oxidative status of growing rabbits. Italian Journal of Animal Science 9, 338–343.Google Scholar
Archetti, I, Tittarelli, C, Cerioli, M, Brivio, R, Grilli, G and Lavazza, A 2008. Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial farms in northern Italy. In Proceedings of 9th World Rabbit Congress, 10–13 June, Verona, Italy, pp. 1147–1152.Google Scholar
Barcz, E, Sommer, E, Nartowska, J, Bałan, BJ, Chorostowska-Wynimko, J and Skopiñska-Różewska, E 2007. Influence of Echinacea purpurea intake during pregnancy on fetal growth and tissue angiogenic activity. Folia Histochemica Et Cytobiologica 45, 35–39.Google ScholarPubMed
Barnes, J, Anderson, LA, Gibbons, S and Phillipson, JD 2005. Echinacea species (Echinacea angustifolia (DC.) Hell., Echinacea pallida (Nutt.) Nutt., Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology 57, 929–954.CrossRefGoogle ScholarPubMed
Barrett, B 2003. Medicinal properties of Echinacea: a critical review. Phytomedicine 10, 66–86.CrossRefGoogle ScholarPubMed
Böhmer, BM, Salisch, H, Paulicks, BR and Roth, FX 2009. Echinacea purpurea as a potential immunostimulatory feed additive in laying hens and fattening pigs by intermittent application. Livestock Science 122, 81–85.CrossRefGoogle Scholar
Cheminat, A, Zawatzky, R, Becker, H and Brouillard, R 1988. Caffeoyl conjugates from Echinacea species: structures and biological activity. Phytochemistry 27, 2787–2794.CrossRefGoogle Scholar
Chow, G, Johns, T and Miller, SC 2006. Dietary Echinacea purpurea during murine pregnancy: effect on maternal hemopoiesis and fetal growth. Biology of the Neonate 89, 133–138.CrossRefGoogle ScholarPubMed
Chrastinová, L, Chrenkova, M, Laukova, A, Poláčiková, M, Simonová, M, Szabóová, R, Strompfová, V, Ondruška, L, Chlebec, I, Parkányi, V, Rafay, J and Vasilkková, Z 2010. Influence of selected phytoadditives and probiotics on zootechnical performance, caecal parameters and meet quality of rabbits. Archivos de Zootecnia 13, 30–35.Google Scholar
Cincotta, R, Balloch, A, Metz, J, Layton, J and Leischke, G 1995. Physiological neutrophilia of pregnancy is not associated with a rise in plasma granulocyte colony-stimulating factor (G-CSF). American Journal of Hematology 48, 288.CrossRefGoogle Scholar
Cundell, DR, Matrone, MA, Rarjaczak, P and Pierce, JD 2003. The effect of aerial parts of Echinacea on the circulating white cell levels and selected immune function of the aging male Spague–Dawley rat. Intern. Immunopharmacology 3, 1041–1048.CrossRefGoogle Scholar
Dalby-Brown, L, Barsett, H, Landbo, AK, Meyer, AS and Molgaard, P 2005. Synergistic antioxidative effects of alkamides, caffeic acid derivatives, and polysaccharide fractions from Echinacea purpurea on in vitro oxidation of human low-density lipoproteins. Journal of Agricultural and Food Chemistry 53, 9413–9423.CrossRefGoogle ScholarPubMed
DeRijk, EP, Esch, E and Flik, G 2002. Pregnancy dating in the rat: placental morphology and maternal blood parameters. Toxicologic Pathology 30, 271–282.CrossRefGoogle Scholar
Egger, M, Pellett, P, Nicki, K, Geiger, S, Graetz, S, Seifert, R, Heilmann, J and König, B 2008. Synthesis and cannabinoid receptor activity of ketoalkenes from Echinacea pallida and non-natural analogues. Chemistry 14, 10978–10984.CrossRefGoogle Scholar
European Economic Community 1972. Analytical determination of starch. Official Journal of European Communities L123, 7.Google Scholar
Gallo, M, Sarkar, M, Au, W, Pietrzak, K, Comas, B, Smith, M, Jaeger, TV, Einarson, A and Koren, G 2000. Pregnancy outcome following gestational exposure to Echinacea: a prospective controlled study. Archives of Internal Medicine 160, 3141–3143.CrossRefGoogle ScholarPubMed
Haneda, R, Mizoguchi, Y, Matsuoka, T, Miuguchi, H, Endon, T, Fukuda, K and Asano, Y 2010. Changes in blood parameters in pregnant Japanese white rabbits. Journal of Toxicology Science 35, 773–778.CrossRefGoogle ScholarPubMed
Hu, C and Kitts, DD 2000. Studies on the antioxidant activity of Echinacea root extract. Journal of Agricultural and Food Chemistry 48, 1466–1472.CrossRefGoogle ScholarPubMed
Hwang, SA, Dasgupta, A and Actor, JK 2004. Cytokine production by non- adherent mouse splenocyte cultures to Echinacea extracts. Clinica Chimica Acta 343, 161–166.CrossRefGoogle ScholarPubMed
IBM SPSS 2012. IBM SPSS statistics 20.0. SPSS Inc., Chicago, IL, USA.Google Scholar
International Rabbit Reproduction Group 2005. Recommendations and guidelines for applied reproduction trials with rabbit does. World Rabbit Science 13, 147–164.Google Scholar
Kovitvadhi, A, Gai, F, Dabbou, S, Ferrocino, I, Rotolo, L, Falzone, M, Vignolini, C, Gennero, MS, Bergagna, S, Dezzutto, D, Barbero, R, Nebbia, P, Rosati, S, Cocolin, L, Zoccarato, I and Gasco, L 2016. Rabbit dietary supplementation with pale purple coneflower. 2. Effects on the performances, bacterial community, blood parameters and immunity of growing rabbits. Animal, doi:10.1017/S1751731115002980.CrossRefGoogle Scholar
Maass, N, Bauer, J, Paulicks, BR, Böhmer, BM and Roth-Maier, DA 2005. Efficiency of Echinacea purpurea on performance and immune status in pigs. Journal of Animal Physiology and Animal Nutrition 89, 244–252.CrossRefGoogle ScholarPubMed
Mizoguchi, Y, Matsuoka, T, Mizuguchi, H, Endoh, T, Kamata, R, Fukuda, K, Ishikawa, T and Asano, Y 2010. Changes in blood parameters in New Zealand white rabbits during pregnancy. Laboratory Animals 44, 33–39.CrossRefGoogle ScholarPubMed
Moscati, L, Dal Bosco, A, Battistacci, L, Cardinali, R, Mugnai, C and Castellini, C 2008. Native immunity and oxidative traits of growing rabbits. World Rabbit Science 16, 213–220.Google Scholar
Osserman, EF and Lawlor, DP 1996. Serum and urinary lysozime (muramidase) in monocytic and monomyelocytic leukaemia. Journal of Experimental Medicine 124, 921–925.CrossRefGoogle Scholar
Ozegbe, C 2001. Influence of pregnancy on some erythrocyte biochemical profiles in the rabbits. African Journal of Biomedical Research 4, 135–137.Google Scholar
Özkan, C, Kaya, A and Akgül, Y 2012. Normal values of haematological and some biochemical parameters in serum and urine of New Zealand white rabbits. World Rabbit Science 20, 253–259.CrossRefGoogle Scholar
Pellati, F, Benvenuti, S, Magro, L and Lasseigne, T 2005. Variability in the composition of antioxidant compounds in Echinacea species by HPLC. Phytochemical Analysis 16, 77–85.CrossRefGoogle ScholarPubMed
Perri, D, Dugoua, JJ, Mills, E and Koren, G 2006. Safety and efficacy of Echinacea (Echinacea angustifolia, E.purpurea and E.pallida) during pregnancy and lactation. Canadian Journal of Clinical Pharmacology 13, 262–267.Google ScholarPubMed
Randolph, RK, Gellenbeck, K, Stonebrook, K, Brovelli, E, Qian, Y, Bankaitis-Davis, D and Cheronis, J 2003. Regulation of human immune gene expression as influenced by a commercial blended Echinacea product: preliminary studies. Experimental Biology and Medecine (Maywood) 228, 1051–1056.CrossRefGoogle ScholarPubMed
Speroni, E, Govoni, P, Guizzardi, S, Renzulli, C and Guerra, MC 2002. Anti-inflammatory and cicatrising activity of Echinacea pallida Nutt. root extract. Journal of Ethnopharmacology 79, 265–272.CrossRefGoogle ScholarPubMed
Tizard, IR 2013. Veterinary immunology, 9th edition. Elsevier Saunders, St. Louis, MO, USA.Google Scholar
Van Soest, P, Robertson, J and Lewis, B 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.CrossRefGoogle ScholarPubMed
Wells, MY, Decobecq, CP, Decouvelaere, DM, Justice, C and Guttin, P 1999. Changes in clinical pathology parameters during gestation in the New Zealand white rabbit. Toxicologic Pathology 27, 370–379.CrossRefGoogle ScholarPubMed
Whitehead, MT, Martin, TD, Scheett, TP and Webster, MJ 2007. The effect of 4 week of oral Echinacea supplementation on serum erythropoietin and indices of erythropoietic status. International Journal of Sport Nutrition and Exercise Metabolism 17, 378–390.CrossRefGoogle Scholar
Wieslaw, PSK, Charon, M, Winnicka, A and Gruszczyñska, J 2006. Relationship between blood lymphocyte phenotype, drb1 (mhc class ii) gene polymorphism and somatic cell count in ewe milk. Bulletin of the Veterinary Institute in Pulawy 50, 73–77.Google Scholar
Zhai, Z, Liu, Y, Wu, L, Senchina, DS, Wurtele, ES, Murphy, PA, Kohut, ML and Cunnick, JE 2007. Enhancement of innate and adaptive immune functions by multiple Echinacea species. Journal of Medicinal Food 10, 423–434.CrossRefGoogle ScholarPubMed