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Polymorphism identification, RH mapping and association of placental lactogen gene with milk production traits of dairy cows

Published online by Cambridge University Press:  01 January 2009

J. Zhang ,
D. X. Sun ,
J. E. Womack ,
Y. C. Wang ,
Y. Yu ,
R. Liu  and
Y. Zhang
J. Zhang*
Affiliation:
Department of Animal Genetics and Breeding, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, China Agricultural University, Beijing 100094, China
D. X. Sun
Affiliation:
Department of Animal Genetics and Breeding, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, China Agricultural University, Beijing 100094, China
J. E. Womack
Affiliation:
Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USA
Y. C. Wang
Affiliation:
Department of Animal Genetics and Breeding, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, China Agricultural University, Beijing 100094, China
Y. Yu
Affiliation:
Department of Animal Genetics and Breeding, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, China Agricultural University, Beijing 100094, China
R. Liu
Affiliation:
Department of Animal Genetics and Breeding, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, China Agricultural University, Beijing 100094, China
Y. Zhang*
Affiliation:
Department of Animal Genetics and Breeding, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, China Agricultural University, Beijing 100094, China
*
E-mail: [email protected]
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Abstract

Bovine placental lactogen (bPL) is structurally related to prolactin (PRL) and growth hormone (GH). In synergism with steroid and thyroid hormones, bPL is crucial in stimulating the development of the mammary gland, mammary cell differentiation and function. To further explore whether bPL gene is associated with milk production traits, we herein analyzed single-nucleotide polymorphisms (SNPs) within eight regions of bPL gene, which are potentially associated with five milk production traits on 1028 Chinese Holstein cows. Among these, two SNPs, NT7409(T–C) and Nt11246(G–A), were identified. The former is within exon 2; it induces an alteration of amino acid from Val to Ala. The later is within exon 4. It is a synonymous mutation. We found that there were significant associations between NT7409(T–C) and milk and protein yield. Cows of the AA genotype yielded less milk (P = 0.001) and less protein (P = 0.003) than those of genotypes AB and BB. However, on the NT11246(G–A) locus, no significant association was observed in the five milk production traits studied. In addition, bPL has been localized near markers RM185 and CC549051 with a distance of 23.2 cR on BTA 23. It is at the same position as the region including quantitative trait loci (QTLs) affecting milk and protein yields by previous linkage analysis. In summary, our findings demonstrated that the SNP within exon 2 of bPL (NT7409(T–C)) is associated with two milk production traits, and this provided further evidence that bPL could be a major gene-controlling milk production trait in Holstein dairy cattle.

Keywords

bPLassociation analysisRH mappingdairy cows
Type
Full Paper
Information
animal , Volume 3 , Issue 1 , January 2009 , pp. 1 - 5
Copyright
Copyright © The Animal Consortium 2008

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