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Ovarian follicular development, lipid peroxidation, antioxidative status and immune response in laying hens fed fish oil-supplemented diets to produce n-3-enriched eggs*

Published online by Cambridge University Press:  01 January 2008

T. Ebeid ,
Y. Eid ,
A. Saleh  and
H. Abd El-Hamid
T. Ebeid
Affiliation:
Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, 33516 Kafrelsheik, Egypt
Y. Eid*
Affiliation:
Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, 33516 Kafrelsheik, Egypt
A. Saleh
Affiliation:
Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, 33516 Kafrelsheik, Egypt
H. Abd El-Hamid
Affiliation:
Poultry Disease Department, Faculty of Veterinary Medicine, Alexandria University, Idfena, Egypt
*
E-mail: [email protected]
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Abstract

The objective of the present study was to research the effect of feeding laying hens fish oil-supplemented diets to produce n-3-enriched eggs on their ovarian follicular development, serum lipid peroxidation, antioxidative status and immune response. A total of 105 white Bovens hens at 24 weeks of age were housed in cages in an open-sided building under a 16 h light : 8 h dark lighting schedule. Birds were randomly divided into five treatments and were fed, ad libitum, diets containing 0% (control), 1.25%, 2.5%, 3.5% or 5.0% fish oil from 24 to 36 weeks of age. Egg production and weight were recorded. By weeks 35 and 36 of age 15 eggs were taken at random from each treatment to determine the yolk lipid profile and cholesterol content. At the end of the experimental period, 10 females from each treatment were randomly chosen, anaesthetised and killed by decapitation. Ovary and oviduct samples were immediately weighted and ovarian follicles were classified. Serum thiobarbituric acid-reactive substance (TBARS), hepatic TBARS and hepatic glutathione peroxidase (GSH-Px) activity were measured. No clear trend was observed concerning egg production and egg yolk cholesterol. As dietary fish oil levels increased, n-3-polyunsaturated fatty acids (n-3 PUFA) increased, whereas n-6 PUFA tended to decrease in yolk lipids. No negative effects were detected in ovary and oviduct weights, expressed in both absolute terms and relative to body weight. The numbers and total weights of large yellow follicles (LYF) in the ovary were not significantly affected by fish oil supplementation. Low levels (1.25% to 2.5%) of fish oil reduced both plasma and hepatic TBARS and enhanced GSH-Px activity. It is also interesting to note that inclusion of 2.5% fish oil in laying hen diets enhanced the antibody titre in laying hens. Therefore, it could be concluded that inclusion of fish oil in laying hen diets at moderate levels increased the n-3 fatty acids content in eggs, improved antioxidative status, enhanced the antibody response and did not have a negative influence on the different reproductive morphology parameters in laying hens.

Keywords

antioxidative systemsimmunityn-3 PUFAovaries
Type
Full Paper
Information
animal , Volume 2 , Issue 1 , January 2008 , pp. 84 - 91
Copyright
Copyright © The Animal Consortium 2008

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Footnotes

*

A part of the data was presented in the 12th European Poultry Conference 10–14 September 2006, Verona, Italy.

References

Arthur, JR 2000. The glutathione peroxidases. Cellular and Molecular Life Sciences 57, 1825–1835.CrossRefGoogle ScholarPubMed
Basmacioglu, H, Cabuk, M, Unal, K, Ozkan, K, Akkan, S, Yalcin, H 2003. Effects of dietary fish oil and flax seed on cholesterol and fatty acid composition of egg yolk and blood parameters of laying hens. South African Journal of Animal Science 33, 266–273.Google Scholar
Baucells, MD, Crespo, N, Barroeta, AC, Lo’pez-Ferrer, S, Grashorn, MA 2000. Incorporation of different polyunsaturated fatty acids into eggs. Poultry Science 79, 51–59.CrossRefGoogle ScholarPubMed
Constant, J 2004. The role of eggs, margarines and fish oils in the nutritional management of coronary artery disease and strokes. The Keio Journal of Medicine 53, 131–136.CrossRefGoogle ScholarPubMed
Cortinas, L, Galobart, J, Barroeta, AC, Baucells, MD, Grashorn, MA 2003. Change in α-tocopherol contents, lipid oxidation and fatty acid profile in eggs enriched with linolenic acid or very long-chain ω3 polyunsaturated fatty acids after different processing methods. Journal of the Science of Food and Agriculture 83, 820–829.CrossRefGoogle Scholar
Crespo, N, Esteve-Garcia, E 2001. Dietary fatty acid profile modifies abdominal fat deposition in broiler chickens. Poultry Science 80, 71–78.CrossRefGoogle ScholarPubMed
De Pablo, MA, Puertollano, MA, de Alvarez de Cienfuegos, G 2002. Biological and clinical significance of lipids as modulators of immune system functions. Clinical and Diagnostic Laboratory Immunology 9, 945–950.Google ScholarPubMed
Endres, S, Meydani, SN, Ghorbani, R, Schindler, R, Dinarello, CA 1993. Dietary supplementation with n-3 fatty acids suppresses interleukin-2 production and mononuclear cell proliferation. Journal of Leukocyte Biology 54, 599–603.CrossRefGoogle ScholarPubMed
Farrell, DJ 1994. The fortification of hen’s eggs with omega-3 long chain fatty acid and their effects in human. In Eggs uses and processing technology (ed. JS Sim and S Nakai), pp. 386–401. CAB International, London, UK.Google Scholar
Friedman, A, Sklan, D 1995. Effect of dietary fatty acids on antibody production and fatty acid composition of lymphoid organs in broiler chickens. Poultry Science 74, 1463–1469.CrossRefGoogle Scholar
Fritsche, KL, Cassity, NA, Huang, SC 1991. Effects of dietary fat source on antibody production and lymphocyte proliferation in chickens. Poultry Science 70, 611–617.CrossRefGoogle ScholarPubMed
Gonzalez, MJ, Gray, JI, Schemmel, RA, JrDugan, L, Welsch, CW 1992. Lipid peroxidation products are elevated in fish oil diets even in the presence of added antioxidants. Journal of Nutrition 122, 2190–2195.CrossRefGoogle ScholarPubMed
Gonzalez-Esquerra, R, Leeson, S 2000. Effect of feeding hens regular or deodorized menhaden oil on production parameters, yolk fatty acid profile and sensory quality of eggs. Poultry Science 79, 1597–1602.CrossRefGoogle ScholarPubMed
Hargis, PS 1988. Modifying egg yolk cholesterol in the domestic fowl-a review. World’s Poultry Science Journal 44, 17–29.CrossRefGoogle Scholar
Huang, ZB, Leibovitz, H, Lee, CM, Miller, R 1990. Effect of dietary fish oil on ω-3 fatty acid levels in chicken eggs and thigh flesh. Journal of Agriculture and Food Chemistry 38, 743–747.CrossRefGoogle Scholar
Ibrahim, W, Lee, US, Yeh, CC, Szabo, J, Bruckner, G, Chow, CK 1997. Oxidative stress and antioxidant status in mouse liver: effects of dietary lipid, vitamin E and iron. The Journal of Nutrition 127, 1401–1406.CrossRefGoogle ScholarPubMed
Korver, DR, Klasing, KC 1997. Dietary fish oil alters specific and inflammatory immune responses in chicks. The Journal of Nutrition 127, 2039–2046.CrossRefGoogle ScholarPubMed
Leskanich, CO, Noble, RC 1997. Manipulation of the n-3 polyunsaturated fatty acid composition of avian eggs and meat. World’s Poultry Science Journal 53, 155–183.CrossRefGoogle Scholar
Levander, OA, Deloach, DP, Morris, VC, Moser, PB 1983. Platelet glutathione peroxidase activity as an index of selenium status in rats. The Journal of Nutrition 113, 55–63.CrossRefGoogle ScholarPubMed
Lewis, NM, Seburg, S, Flanagan, NL 2000. Enriched eggs as a source of n-3 polyunsaturated fatty acids for humans. Poultry Science 79, 971–974.CrossRefGoogle ScholarPubMed
Meluzzi, A, Sirri, F, Manfreda, G, Tallarico, N, Franchini, A 2000. Effects of dietary vitamin E on the quality of table eggs enriched with n-3 long-chain fatty acids. Poultry Science 79, 539–545.CrossRefGoogle ScholarPubMed
Nash, DM, Hamilton, RMG, Hulan, HW 1995. The effect of dietary herring meal on the omega-3-fatty-acid content of plasma and egg-yolk lipids of laying hens. Canadian Journal of Animal Science 75, 247–253.CrossRefGoogle Scholar
Nenseter, MS, Rustan, AC, Lund-Katz, S, Søyland, E, Mælandsmo, G, Phillips, MC, Drevon, CA 1992. Effect of dietary supplementation with n-3 polyunsaturated fatty acids on physical properties and metabolism of low density lipoprotein in humans. Arteriosclerosis Thrombosis and Vascular Biology 12, 369–379.CrossRefGoogle ScholarPubMed
Pappas, AC, Acamovic, T, Sparks, NHC, Surai, PF, McDevitt, RM 2005. Effects of supplementing broiler breeder diets with organic selenium and polyunsaturated fatty acids on egg quality during storage. Poultry Science 84, 865–874.CrossRefGoogle ScholarPubMed
Parmentier, HK, Nieuwland, MGB, Barwegen, MW, Kwakkel, RP, Chrama, JW 1997. Dietary unsaturated fatty acids affect antibody responses and growth of chickens divergently selected for humoral responses to sheep red blood cells. Poultry Science 76, 1164–1171.CrossRefGoogle ScholarPubMed
Petit, HV, Dewhurst, RJ, Scollan, ND, Proulx, JG, Khalid, M, Haresign, W, Twagiramungu, H, Mann, GE 2002. Milk production and composition, ovarian function and prostaglandin secretion of dairy cows fed omega-3 fats. Journal of Dairy Science 85, 889–899.CrossRefGoogle ScholarPubMed
Radwan, SS 1978. Coupling of two dimensional thin layer chromatography with gas chromatography of the quantitative analysis of lipids classed and their constituent fatty acids. Journal of Chromatographic Science 16, 538–542.CrossRefGoogle Scholar
Renema, RA, Robinson, FE, Melnychuk, VL, Hardin, RT, Bagley, LG, Emmerson, DA, Blackman, JR 1995. The use of feed restriction for improving reproductive traits in male-line large white turkey hens. 2. Ovary morphology and laying trait characteristics. Poultry Science 74, 102–120.CrossRefGoogle Scholar
Richard, MJ, Portal, B, Meo, J, Coudray, C, Hadjian, A, Favier, A 1992. Malondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid. Clinical Chemistry 38, 704–709.CrossRefGoogle ScholarPubMed
Robinson, RS, Pushpakumara, PGA, Cheng, Z, Peters, AR, Abayasekara, DRE, Wathes, DC 2002. Effects of dietary polyunsaturated fatty acids on ovarian and uterine function in lactating dairy cows. Reproduction Cambridge England 124, 119–131.CrossRefGoogle ScholarPubMed
Scheideler, SE, Froning, GW 1996. The combined influence of dietary flaxseed variety, level, form and storage conditions on egg production and composition among vitamin E-supplemented hens. Poultry Science 75, 1221–1226.CrossRefGoogle ScholarPubMed
Schreiner, M, Hulan, HW, Razzazi-Fazeli, E, Bohm, J, Iben, C 2004. Feeding laying hens seal blubber oil: effects on egg yolk incorporation, stereospecific distribution of omega-3 fatty acids and sensory aspects. Poultry Science 83, 462–473.CrossRefGoogle ScholarPubMed
Shimizu, Y, Arai, K, Ise, S, Shimasaki, H 2001. Dietary fish oil for hens affects the fatty acid composition of egg yolk phospholipids and gives a valuable food with an ideal balance of n-6 and n-3 essential fatty acids for human nutrition. Journal of Oleo Science 50, 797–803.CrossRefGoogle Scholar
Simopoulos, AP 2000. Human requirement for n-3 polyunsaturated fatty acids. Poultry Science 79, 961–970.CrossRefGoogle ScholarPubMed
Statistical Packages for the Social Sciences 2001. Statistical software for windows version 11.0 Microsoft. SPSS, Chicago, IL, USA.Google Scholar
Surette, ME, Whelan, J, Lu, G, Hardard’ottir, I, Kinsella, JE 1995. Dietary n-3 polyunsaturated fatty acids modify Syrian hamster platelet and macrophage phospholipid fatty acyl composition and eicosanoid synthesis: a controlled study. Biochimica et Biophysica Acta 1255, 185–191.CrossRefGoogle ScholarPubMed
Thomas, MG, Bao, B, Williams, GL 1997. Dietary fats varying in their fatty acid composition differentially influence follicular growth in cows fed isoenergetic diets. Journal of Animal Science 75, 2512–2519.CrossRefGoogle ScholarPubMed
Van Elswyk, ME 1997. Nutritional and physiological effects of flax seed in diets for laying fowl. World’s Poultry Science Journal 53, 253–264.CrossRefGoogle Scholar
Wang, YW, Field, CJ, Sim, JS 2000. Dietary polyunsaturated fatty acids alter lymphocyte subset proportion and proliferation, serum immunoglobulin G concentration and immune tissue development in chicks. Poultry Science 79, 1741–1748.CrossRefGoogle ScholarPubMed
Watson, D 1960. A simple method for the determination of serum cholesterol. Clinica Chimica Acta 5, 637–643.CrossRefGoogle ScholarPubMed
Wegmann, TG, Smithies, O 1966. A simple hemagglutination system requiring small amounts of red cells and antibodies. Transfusion 6, 67–73.CrossRefGoogle Scholar
Yuan, YV, Kitts, DD 2002. Dietary fat source and cholesterol interactions alter plasma lipids and tissue susceptibility to oxidation in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rats. Molecular and Cellular Biochemistry 232, 33–47.CrossRefGoogle ScholarPubMed