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Modulation of porcine cytochrome P450 enzyme activities by surgical castration and immunocastration

Published online by Cambridge University Press:  01 August 2009

G. Zamaratskaia ,
V. Zlabek ,
G. Chen  and
A. Madej
G. Zamaratskaia*
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala SE-750 07, Sweden
V. Zlabek
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala SE-750 07, Sweden University of South Bohemia, Ceske Budejovice, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic
G. Chen
Affiliation:
Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
A. Madej
Affiliation:
Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala SE-750 07, Sweden
*
†E-mail: [email protected]
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Abstract

The present study aimed to evaluate some cytochrome P450 metabolic enzyme activities in hepatic microsomes prepared from entire male pigs (uncastrated pigs), surgically castrated pigs and pigs immunized against gonadotropin-releasing hormone (immunocastrated pigs). The activities of the following enzymes were measured: ethoxyresorufin O-deethylase (EROD, CYP1A1/1A2), methoxyresorufin O-deethylase (MROD, CYP1A2), pentoxyresorufin O-depentylase (PROD, CYP2B), coumarin hydroxylase (COH, CYP2A) and p-nitrophenol hydroxylase (PNPH, CYP2A/2E1). The total cytochrome P450 contents were not affected by either surgical or immunocastration. Hepatic microsomal activities for EROD, PROD, COH and PNPH were lower in entire male pigs compared with surgically castrated and immunocastrated pigs (P < 0.05). Surgically and immunocastrated male pigs were similar with respect to EROD, MROD, PROD and COH activities (P > 0.05), whereas surgically castrated pigs exhibited lower PNPH activity compared with immunocastrated pigs (P = 0.029). The effect of different concentrations of testicular steroids – testosterone, 17β-estradiol, free estrone and androstenone – on enzyme activities was evaluated by in vitro microsomal study. Testosterone at the concentration of 8 pmol/ml inhibited EROD activities and estradiol-17β at the concentration of 1.8 pmol/ml inhibited PROD activities in hepatic microsomes from surgically castrated pigs. The highest concentration of androstenone (7520 pmol/ml) inhibited COH activities, whereas a 42-fold lower concentration of androstenone (180 pmol/ml) stimulated COH activities in surgically castrated pigs. Both free estrone (3.5 pmol/ml) and androstenone (55 pmol/ml) inhibited EROD activities in microsomes from entire male pigs. Stimulation of COH activities by the highest dose of free estrone (18 pmol/ml) was recorded in microsomes from entire male pigs. However, these effects of steroids were not concentration-dependent and the maximum extent did not exceed ±15% variation compared with the controls. There was no inhibition of PNPH activities in the hepatic microsomes from either entire or castrated pigs. In conclusion, we showed that EROD, PROD, COH and PNPH activities were lower in entire male pigs compared with those in surgically and immunocastrated pigs. Direct inhibition by the testicular steroids – testosterone, 17β-estradiol, free estrone and androstenone – was not the primary cause of the reduced enzyme activities.

Keywords

castrationcytochrome P450porcine liver microsomessteroids
Type
Full Paper
Information
animal , Volume 3 , Issue 8 , August 2009 , pp. 1124 - 1132
Copyright
Copyright © The Animal Consortium 2009

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