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Modification of abomasum contractility by flavonoids present in ruminants diet: in vitro study

Published online by Cambridge University Press:  04 April 2016

M. Mendel ,
M. Chłopecka ,
N. Dziekan  and
W. Karlik
M. Mendel*
Affiliation:
Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 8, Ciszewskiego St., 02-786 Warsaw, Poland
M. Chłopecka
Affiliation:
Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 8, Ciszewskiego St., 02-786 Warsaw, Poland
N. Dziekan
Affiliation:
Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 8, Ciszewskiego St., 02-786 Warsaw, Poland
W. Karlik
Affiliation:
Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 8, Ciszewskiego St., 02-786 Warsaw, Poland
*
† E-mail: [email protected]
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Abstract

Flavonoid supplementation is likely to be beneficial in improving rumen fermentation and in reducing the incidence of rumen acidosis and bloat. Flavonoids are also said to increase the metabolic performance during the peripartum period. Ruminants are constantly exposed to flavonoids present in feed. However, it is not clear if these phytochemicals can affect the activity of the gut smooth muscle. Therefore, the aim of the study was to verify the effect of three flavonoids on bovine isolated abomasum smooth muscle. The study was carried out on bovine isolated circular and longitudinal abomasal smooth muscle specimens. All experiments were conducted under isometric conditions. The effect of apigenin, luteolin and quercetin (0.001 to 100 µM) was evaluated on acetylcholine-precontracted preparations. The effect of multiple, but not cumulative, treatment and single treatment with each flavonoid on abomasum strips was compared. Apigenin (0.1 to 100 µM) dose-dependently showed myorelaxation effects. Luteolin and quercetin applied in low doses increased the force of the ACh-evoked reaction. However, if used in high doses in experiments testing a wide range of concentrations, their contractile effect either declined (luteolin) or was replaced by an antispasmodic effect (quercetin). Surprisingly, the reaction induced by flavonoids after repeated exposure to the same phytochemical was not reproducible in experiments testing only single exposure of abomasum strips to the same flavonoid used in a high concentration. Taking into account the physicochemical properties of flavonoids, this data suggests the ability of flavonoids to interfere with cell membranes and, subsequently, to modify their responsiveness. Assuming ruminant supplementation with luteolin or quercetin or their presence in daily pasture, a reduction of the likelihood of abomasum dysmotility should be expected.

Keywords

flavonoidsabomasumsmooth muscle contractilitybovine
Type
Research Article
Information
animal , Volume 10 , Issue 9 , September 2016 , pp. 1431 - 1438
Copyright
© The Animal Consortium 2016 

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References

Amira, S, Rotondo, A and Mule, F 2008. Relaxant effects of flavonoids on the mouse isolated stomach: structure-activity relationships. European Journal of Pharmacology 599, 126–130.CrossRefGoogle ScholarPubMed
Balcells, J, Aris, A, Serrano, A, Serad, AR, Crespo, J and Devant, M 2012. Effects of an extract of plant flavonoids (Bioflavex) on rumen fermentation and performance in heifers fed high-concentrate diets. Journal of Animal Science 90, 4975–4984.Google Scholar
Bashir, S, Janbaz, KH, Jabeen, Q and Gilani, AH 2006. Studies on spasmogenic and spasmolytic activities of Calendula officinalis flowers. Phytotherapy Research 20, 906–910.Google Scholar
Berger, LM, Wein, S, Blank, R, Metges, CC and Wolffram, S 2012. Bioavailability of the flavonols quercetin in cows after intraruminal application of quercetin aglycone and rutin. Journal of Dairy Science 95, 5047–5055.Google Scholar
Bernabucci, U, Ronchi, B, Lacetera, N and Nardone, A 2005. Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science 88, 2017–2026.Google Scholar
Besle, JM, Viala, D, Martin, B, Pradel, P, Meunier, B, Berdague, JL, Fraisse, DF, Lamaison, JL and Coulon, JB 2010. Ultraviolet-absorbing compounds in milk are related to forage polyphenols. Journal of Dairy Science 93, 2846–2856.CrossRefGoogle ScholarPubMed
de Boer, VCJ, Dihal, AA, van der Woude, H, Arts, ICW, Wolffram, S, Alink, GM, Rietjens, MCM, Keijer, J and Hollman, PCH 2005. Tissue distribution of quercetin in rats and pigs. Journal of Nutrition 135, 1718–1725.Google Scholar
Capasso, A, Pinto, A, Mascolo, N, Autore, G and Capasso, F 1991a. Reduction of agonist-induced contractions of guinea-pig isolated ileum by flavonoids. Phytotherapy Research 5, 85–87.Google Scholar
Capasso, A, Pinto, A, Sorrentino, R and Capasso, F 1991b. Inhibitory effects of quercetin and other flavonoids on electrically-induced contractions of guinea pig isolated ileum . Journal of Ethnopharmacology 34, 279–281.Google Scholar
Dar, A and Channa, S 1999. Calcium antagonistic activity of Bacopa monniera on vascular and intestinal smooth muscles of rabbit and guinea-pig. Journal of Ethnopharmacology 66, 167–174.CrossRefGoogle ScholarPubMed
Di Carlo, G, Autore, G, Izzo, AA, Mascolo, N, Viola, P, Diurno, MV and Capasso, F 1993. Inhibition of intestinal motility and secretion by flavonoids in mice and rats: structure-activity relationships. Journal of Pharmacy and Pharmacology 45, 1054–1059.Google Scholar
Di Carlo, G, Mascolo, N, Izzo, AA and Capasso, F 1994. Effects of quercetin on the gastrointestinal tract in rats and mice. Phytotherapy Research 8, 42–45.Google Scholar
Doll, K 2007. Aktuelles zu den Ursachen der Labmagenverlagerung. Zuchtungskunde 79, 59–69.Google Scholar
Duarte, J, Pérez Vizcaíno, F, Utrilla, P, Jiménez, J, Tamargo, J and Zarzuelo, A 1993. Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships. General Pharmacology 24, 857–862.CrossRefGoogle ScholarPubMed
EU Regulation No. 1831/2003. EU Regulation No. 1831/2003 of the European Parliament and of Council of 22 September 2003. Official Journal of the European Union L268, 29.Google Scholar
Geishauser, T, Reiche, D and Schemnann, M 1998. In vitro motility disorders associated with displaced abomasum in dairy cows. Neurogastroenterology and Motility 10, 395–401.CrossRefGoogle ScholarPubMed
Gharzouli, K and Holzer, P 2004. Inhibition of guinea-pig intestinal peristalsis by the flavonoids quercetin, naringenin, apigenin and genistein. Pharmacology 70, 5–14.Google Scholar
Ghayur, MN, Khan, H and Gilani, AH 2007. Antispasmodic, bronchodilator and vasodilator activities of (+)-catechin, a naturally occurring flavonoid. Archives of Pharmacology Research 30, 970–975.Google Scholar
Githiori, JB, Athanasiadou, S and Thamsborg, SM 2006. Use of plants in novel approaches for control of gastrointestinal helminths in livestock with emphasis on small ruminants. Veterinary Parasitology 139, 308–320.Google Scholar
Gohlke, A, Ingelmann, AJ, Nürnberg, G, Wolffram, S and Metges, CC 2011. Bioavailability of duodenal administered flavonoids and acute effects on milk yield in high-yielding German-Holstein cows: Preeliminary results. 3rd COST Feed for Health Conference Copenhagen, Denmark. Retrieved June 11, 2012, from http://www.feed-forhealth.org/default.asp?ZNT=S0T1O856.Google Scholar
Hammad, HA and Abdalla, SS 1997. Pharmacological effects of selected flavonoids on rat isolated ileum: structure-activity relationship. General Pharmacology 28, 767–771.Google Scholar
Harborne, JB and Williams, CA 2000. Advances in flavonoid research since 1992. Phytochemistry 55, 481–504.CrossRefGoogle ScholarPubMed
Hendrich, AB 2006. Flavonoid-membrane interactions: possible consequences for biological effects of some polyphenolic compounds. Acta Pharmacolologica Sinica 27, 27–40.Google Scholar
Herrera, MD, Zarzuelo, A, Jimenez, J, Marhuenda, E and Duarte, J 1996. Effects of flavonoids on rat aortic smooth muscle contractility: structure-activity relationships. General Pharmacology 27, 273–277.Google Scholar
Je, HD, Kim, HD and La, HO 2014. The inhibitory effect of apigenin on the agonist-induced regulation of vascular contractility via calcium desensitization-related pathway. Biomolecules and Therapeutics 22, 100–105.Google Scholar
Khayyal, MT, El-Ghazaly, MA, Kenway, SA, El-Nasar, MS, Mahran, LG, Kafafi, YAH and Okpanyi, SN 2001. Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination. Arzneimittel Forschung/Drug Research 51, 545–553.Google Scholar
Kobayashi, Y, Miyazawa, M, Kamei, A, Abe, K and Kojima, T 2010. Ameliorative effects of mulberry (Morus alba L.) leaves on hyperlipidemia in rats fed a high-fat diet: induction of fatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stress. Bioscience Biotechnology Biochemistry 74, 2385–2395.Google Scholar
Morales, MA, Tortoriello, J, Meckes, M, Paz, D and Lozoya, X 1994. Calcium-antagonist effect of quercetin and its relation with the spasmolytic properties of Psidium guajava L. Archives in Medicine Research 25, 17–21.Google Scholar
Nielsen, B and Hansen, H 2004. Effect of grape pomace rich in flavonoids and antioxidants on production parameters in dairy production. Journal of Animal and Feed Science 13, 535–538.Google Scholar
Nijveldt, RJ, van Nood, E, van Hoorn, DEC, Boelens, PG, van Norren, K and van Leeuwen, PAM 2001. Flavonoids: a review of probable mechanisms of action and potential applications. American Journal of Clinical Nutrition 74, 418–425.Google Scholar
Reynaud, A, Fraisse, D, Cornu, A, Farruggia, A, Pujos-Guillot, E, Besle, JM, Martin, B, Lamaison, JL, Paquet, D, Doreau, M and Graulet, B 2010. Variation in content and composition of phenolic compounds in permanent pastures according to botanical variation. Journal of Agricultural and Food Chemistry 58, 5485–5494.Google Scholar
Rocha, BS, Gago, B, Barbosa, RM and Laranjinha, J 2009. Dietary polyphenols generate nitric oxide from nitrite in the stomach and induce smooth muscle relaxation. Toxicology 265, 41–48.CrossRefGoogle ScholarPubMed
Thiyagarajah, P, Kuttan, SC, Lim, SC, Teo, TS and Das, NP 1991. Effect of myricetin and other flavonoids on the liver plasma membrane Ca2+pump; Kinetics and structure-function relationships. Biochemical Pharmacology 41, 669–675.Google Scholar
Vogalis, F 2000. Potassium channels in gastrointestinal smooth muscle. Journal of Autonomic Pharmacology 20, 207–219.CrossRefGoogle ScholarPubMed
Wang, LCh, Garcia-Rivera, J and Burries, RH 1961. Metabolism of nitrate by cattle. Biochemistry Journal 81, 237–242.Google Scholar
Yang, ZH, Pan, A, Zuo, WL, Guo, JH and Zhou, WL 2014. Relaxant effect of flavonoid naringenin on contractile activity of rat colonic smooth muscle. Journal of Ethnopharmacology 155, 1177–1183.CrossRefGoogle ScholarPubMed