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Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Published online by Cambridge University Press:  23 February 2012

N. Silanikove ,
A. Rauch-Cohen ,
F. Shapiro ,
A. Arieli ,
U. Merin  and
G. Leitner
N. Silanikove*
Affiliation:
Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
A. Rauch-Cohen
Affiliation:
Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
F. Shapiro
Affiliation:
Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
A. Arieli
Affiliation:
Department of Animal Science, Faculty of Agricultural, Food and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76-100, Israel
U. Merin
Affiliation:
Food Quality and Safety, Institute of Postharvest and Food Sciences, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
G. Leitner
Affiliation:
National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 12, Bet Dagan 50250, Israel
*
†E-mail: [email protected]
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Abstract

The aim of this work was to study the effects of mastitis induced by intramammary lipopolysaccharide (LPS) challenge on milk oxidative stability, as well as to understand the underlying biochemical processes that cause such changes. LPS challenge was associated with nitric oxide burst from the surrounding mammary epithelial cells and consequently induced nitrosative stress that was induced by the formation of NO2• from nitrite by lactoperoxidase. This response was associated with an ∼3-fold increased formation of hazardous compounds: nitrotyrosines, carbonyls and lipid peroxides. We sustained the involvement of xanthine oxidase as a major source of hydrogen peroxide. In consistent with previous findings, catalase has been shown to play a major role in modulating the nitrosative stress by oxidizing nitrite to nitrate. The current hygienic quality criteria cannot detect mixing of low-quality milk, such as milk with high somatic cells, and nitrite with high-quality milk. Thus, development of an improved quality control methodology may be important for the production of high-quality milk.

Keywords

milkcowmastitisNO and NO2•plasmin system
Type
Full Paper
Information
animal , Volume 6 , Issue 9 , September 2012 , pp. 1451 - 1459
Copyright
Copyright © The Animal Consortium 2012

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