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Intestinal amino acid absorption in lambs fed fresh Lucerne (Medicago sativa) during an established Trichostrongylus colubriformis infection

Published online by Cambridge University Press:  01 July 2008

E. N. Bermingham ,
N. C. Roy ,
I. A. Sutherland ,
G. C. Waghorn ,
B. R. Sinclair ,
J. S. Peters  and
W. C. McNabb
E. N. Bermingham*
Affiliation:
Food, Metabolism & Microbiology, AgResearch Grasslands, Palmerston North 4442, New Zealand
N. C. Roy
Affiliation:
Food, Metabolism & Microbiology, AgResearch Grasslands, Palmerston North 4442, New Zealand
I. A. Sutherland
Affiliation:
Animal Health, AgResearch Hopkirk Research Institute, Palmerston North 4442, New Zealand
G. C. Waghorn
Affiliation:
Food, Metabolism & Microbiology, AgResearch Grasslands, Palmerston North 4442, New Zealand
B. R. Sinclair
Affiliation:
Food, Metabolism & Microbiology, AgResearch Grasslands, Palmerston North 4442, New Zealand
J. S. Peters
Affiliation:
Food, Metabolism & Microbiology, AgResearch Grasslands, Palmerston North 4442, New Zealand
W. C. McNabb
Affiliation:
Food, Metabolism & Microbiology, AgResearch Grasslands, Palmerston North 4442, New Zealand
*
E-mail: [email protected]
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Abstract

The effects of an established Trichostrongylus colubriformis infection on amino acid (AA) absorption from the small intestine and their availability to other tissues were determined in lambs 48 days post infection. The lambs were fed fresh Lucerne (Medicago sativa; ∼800 g dry matter (DM)/day) and dosed with 6000 L3 T. colubriformis larvae for 6 days (n = 5) or kept as parasite free controls (n = 6). Faecal egg production was monitored every second day from day 22 to day 48. A nitrogen (N) balance was conducted on days 35 to 43 after infection, and digesta flow and AA concentration measurements were made on day 44. On day 48 after infection, blood was continuously collected from the mesenteric artery and vein, plasma harvested and AA concentrations measured. Faecal egg production peaked on the 26th day after infection (P < 0.001) and intestinal worm burdens on day 48 were greater (P < 0.001) in the infected lambs. Feed intake and liveweight gain were similar (P > 0.10) between control and infected lambs. Digestibility and flow of DM and N through the digestive tract were also unaffected (P > 0.10) by parasite infection. Despite a trend towards higher abomasal AA flux in the parasitised lambs (P < 0.10), apparent AA absorption from the small intestine and AA availability to other tissues were unaffected (P > 0.10) by infection. These results suggest that an established parasite infection had little effect on the intestinal absorption and availability of AA to other tissues in lambs fed fresh Lucerne.

Keywords

amino aciddigestibilityintestinal absorptionlambsparasitic infection
Type
Full Paper
Information
animal , Volume 2 , Issue 7 , July 2008 , pp. 1037 - 1044
Copyright
Copyright © The Animal Consortium 2008

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References

Barger, IA 1985. The statistical distribution of Trichostrongylid nematodes in grazing lambs. International Journal for Parasitology 15, 645–649.Google Scholar
Barker, IK, Titchen, DA 1982. Gastric dysfunction in sheep infected with Trichostrongylus colubriformis, a nematode inhabiting the small intestine. International Journal for Parasitology 12, 345–356.Google Scholar
Barnes, EH, Dobson, RJ 1990. Population dynamics of Trichostrongylus colubriformis in sheep: mathematical model of worm fecundity. International Journal for Parasitology 20, 375–380.Google Scholar
Bermingham EN 2004. The metabolic cost of an intestinal parasite infection on amino acid kinetics in sheep fed fresh forages. PhD Thesis, Massey University, New Zealand.Google Scholar
Bermingham, EN, Hutchinson, KJ, Revell, DK, Brookes, IM, McNabb, WC 2001. The effect of condensed tannins in sainfoin (Onobrychis viciifolia) and sulla (Hedysarum coronarium) on the digestion of amino acids in sheep. Proceedings of the New Zealand Society of Animal Production 61, 116–119.Google Scholar
Bermingham, EN, McNabb, WC, Sutherland, IA, Waghorn, GC, Sinclair, BR, Treloar, BP, Roy, NC 2006. Whole-body valine and cysteine kinetics and tissue fractional protein synthesis rates in lambs fed Sulla (Hedysarum coronarium) and infected or not infected with adult Trichostrongylus colubriformis. British Journal of Nutrition 96, 28–38.Google Scholar
Bermingham, EN, McNabb, WC, Sutherland, IA, Sinclair, BR, Treloar, BP, Roy, NC 2007a. Whole-body protein turnover and tissue fractional protein synthesis rates in lambs fed Lucerne infected with or without Trichostrongylus colubriformis. Canadian Journal of Animal Science 87, 315–325.Google Scholar
Bermingham, EN, McNabb, WC, Sutherland, IA, Sinclair, BR, Treloar, BP, Roy, NC 2007b. Intestinal, hepatic, splanchnic and hindquarter amino acid and metabolite partitioning during an established Trichostrongylus colubriformis infection in the small intestine of lambs fed fresh Sulla (Hedysarum coronarium). British Journal of Nutrition 98, 1132–1142.Google Scholar
Cheema, KJ, Scofield, AM 1982. Scanning electron microscopy of the intestines of rats infected with Nippostrongylus brasiliensis. International Journal for Parasitology 12, 199–205.Google Scholar
Coop, RL, Angus, KW 1975. The effect of continuous doses of Trichostrongylus colubriformis larvae on the intestinal mucosa of sheep and on liver vitamin A concentration. Parasitology 70, 1–9.Google Scholar
Coop, RL, Kyriazakis, I 1999. Nutrition-parasite interaction. Veterinary Parasitology 84, 187–204.Google Scholar
Familton, AS, McAnulty, RW 1997. Life cycles and development of nematode parasites of ruminants. In Sustainable Control of Internal Parasites in Ruminants (ed. GK Barrell), pp. 67–79. Lincoln University, Canterbury, New Zealand.Google Scholar
Gregory, PC, Wenham, G, Poppi, DP, Coop, RL, MacRae, JC, Miller, SJ 1985. The influence of chronic subclinical infection of Trichostrongylus colubriformis on gastromotility and digesta flow in sheep. Parasitology 91, 381–396.Google Scholar
Huntington, GB, Reynolds, CK, Stroud, BH 1989. Techniques for measuring blood flow in splanchnic tissues of cattle. Journal of Dairy Science 72, 1583–1595.Google Scholar
Janes, AN, Weekes, TEC, Armstrong, DG 1985. Absorption and metabolism of glucose by the mesenteric-drained viscera of sheep fed on dried-grass or ground, maize-based diets. British Journal of Nutrition 54, 449–458.Google Scholar
Katz, ML, Bergman, EN 1969. Simultaneous measurements of hepatic and portal venous blood flow in the sheep and dog. American Journal of Physiology 216, 946–952.Google Scholar
Kimambo, AE, MacRae, JC, Walker, A, Watt, CF, Coop, RL 1988. Effect of prolonged subclinical infection with Trichostrongylus colubriformis on the performance and nitrogen metabolism of growing lambs. Veterinary Parasitology 28, 191–203.Google Scholar
Lobley, GE, Connell, A, Lomax, MA, Brown, DS, Milne, E, Calder, AG, Farningham, DAH 1995. Hepatic detoxification of ammonia in the ovine liver: possible consequences for amino acid catabolism. British Journal of Nutrition 73, 667–685.Google Scholar
MacRae, JC, Lobley, GE 1991. Physiological and metabolic implications of conventional and novel methods for the manipulation of growth and production. Livestock Production Science 27, 43–59.Google Scholar
MacRae, JC, Bruce, LA, Brown, DS, Calder, AG 1997a. Amino acid use by the gastrointestinal tract of sheep given lucerne forage. American Journal of Physiology 36, G1200–G1207.Google Scholar
MacRae, JC, Bruce, LA, Brown, DS, Farningham, DAH, Franklin, M 1997b. Absorption of amino acids from the intestine and their net flux across the mesenteric-, and portal-drained viscera of lambs. Journal of Animal Science 75, 3307–3314.Google Scholar
Mayes, RW, Lamb, CS, Colgrove, PM 1986. The use of dosed herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, Cambridge 107, 161–170.Google Scholar
Ortigues, I, Durand, D 1995. Adaptation of energy metabolism to undernutrition in ewes. Contribution of portal-drained viscera, liver and hindquarters. British Journal of Nutrition 73, 209–226.Google Scholar
Poppi, DP, MacRae, JC, Brewer, A, Coop, RL 1986. Nitrogen transactions in the digestive tract of lambs exposed to the internal parasite, Trichostrongylus colubriformis. British Journal of Nutrition 55, 593–602.Google Scholar
Reeds, PJ, Burrin, DG, Stoll, B, Jahoor, F 2000. Intestinal glutamate metabolism. Journal of Nutrition 130, 978s–982s.Google Scholar
Roseby, FB 1973. Effects of Trichostrongylus colubriformis (Nematoda) on the nutrition and metabolism of sheep. 1. Feed intake, digestion, and utilisation. Australian Journal of Agricultural Research 24, 947–953.Google Scholar
Scofield, AM 1980. Effect of level of infection with Nippostrongylus brasiliensis on intestinal absorption of hexoses in rats. International Journal for Parasitology 10, 375–380.Google Scholar
Shenk, JS, Westerhaus, MO 1991. Population structuring of near infrared spectra and modified partial least squares regression. Crop Science 31, 1694–1696.Google Scholar
Steel, JW, Symons, LEA, Jones, WO 1980. Effects of level of larval intake on the productivity and physiological and metabolic responses of lambs infected with Trichostrongylus colubriformis. Australian Journal of Agricultural Research 31, 821–838.Google Scholar
Steel, JW, Jones, WO, Symons, LEA 1982. Effects of a concurrent infection of Trichostrongylus colubriformis on the productivity and physiological and metabolic responses of lambs infected with Ostertagia circumcincta. Australian Journal of Agricultural Research 33, 131–140.Google Scholar
Sutherland, IA, Brown, AE, Green, RS, Miller, CM, Leathwick, DM 1999a. The immune response of sheep to larval challenge with Ostertagia circumcinta and O. ostertagi. Veterinary Parasitology 84, 125–135.Google Scholar
Sutherland, IA, Leathwick, DM, Green, R, Brown, AE, Miller, CM 1999b. The effect of continuous drug exposure on the immune response to Trichostrongylus colubriformis in sheep. Veterinary Parasitology 80, 261–271.Google Scholar
Sykes, AR, Coop, RL 1976. Intake and utilisation of food by growing lambs with parasitic damage to the small intestine caused by daily dosing with Trichostrongylus colubriformis larvae. Journal of Agricultural Science, Cambridge 86, 507–515.Google Scholar
Sykes, AR, Poppi, DP, Elliot, DC 1988. Effect of concurrent infection with Ostertagia circumcincta and Trichostrongylus colubriformis on the performance of growing lambs consuming fresh forages. Journal of Agricultural Science, Cambridge 110, 531–541.Google Scholar
Symons, LEA, Jones, WO 1975. Skeletal muscle, liver and wool protein synthesis by sheep infected by the nematode Trichostrongylus colubriformis. Australian Journal of Agricultural Research 26, 1063–1072.Google Scholar
Ulyatt, MJ 1981. The feeding value of herbage: can it be improved? New Zealand Agricultural Science 15, 200–205.Google Scholar
van Houtert, MFJ, Barger, IA, Steel, JW, Windon, RG, Emery, DL 1995. Effects of dietary protein intake on responses of young sheep to infection with Trichostrongylus colubriformis. Veterinary Parasitology 56, 163–180.Google Scholar
Waghorn, GC, Ulyatt, MJ, John, A, Fisher, MT 1987. The effects of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus L. British Journal of Nutrition 57, 115–126.Google Scholar
Waghorn, GC, Shelton, ID, McNabb, WC, McCutcheon, SN 1994. Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects. Journal of Agricultural Science (Cambridge) 123, 109–119.Google Scholar
Whitlock, H 1948. Some modifications of the McMaster helminth egg-counting technique and apparatus. Journal of the Council for Scientific Industrial Research, Australia 21, 177–180.Google Scholar
Yu, F, Bruce, LA, Calder, AG, Milne, E, Coop, RL, Jackson, F, Horgan, GW, MacRae, JC 2000. Subclinical infection with the nematode Trichostrongylus colubriformis increases gastrointestinal tract leucine metabolism and reduces availability of leucine for other tissues. Journal of Animal Science 78, 380–390.Google Scholar