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In vitro storage of unfertilized eggs of the Eurasian perch and its effect on egg viability rates and the occurrence of larval malformations

Published online by Cambridge University Press:  07 July 2016

A. M. Samarin ,
D. Żarski ,
K. Palińska-Żarska ,
S. Krejszeff ,
M. Blecha ,
D. Kucharczyk  and
T. Policar
A. M. Samarin*
Affiliation:
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic
D. Żarski
Affiliation:
Department of Aquaculture, Szent István University, Páter Károly u. 1, 2100 Gödöllő, Hungary Department of Lake and River Fisheries, University of Warmia and Mazury, ul. Oczapowskiego 2, 10-719 Olsztyn, Poland
K. Palińska-Żarska
Affiliation:
Department of Lake and River Fisheries, University of Warmia and Mazury, ul. Oczapowskiego 2, 10-719 Olsztyn, Poland
S. Krejszeff
Affiliation:
Department of Lake and River Fisheries, University of Warmia and Mazury, ul. Oczapowskiego 2, 10-719 Olsztyn, Poland
M. Blecha
Affiliation:
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic
D. Kucharczyk
Affiliation:
Department of Lake and River Fisheries, University of Warmia and Mazury, ul. Oczapowskiego 2, 10-719 Olsztyn, Poland
T. Policar
Affiliation:
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic
*
† E-mail: [email protected]
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Abstract

Ova ageing is the most important factor affecting fish egg quality after ovulation. Long-term storage of fish ova, using cryopreservation and vitrification techniques, has been unsuccessful to date. Instead, short-term in vitro ova storage has been used successfully and optimized in some cultured fish species. In vitro ova storage can drastically improve mass production of larvae and juveniles in the hatcheries by providing the possibility of the synchronous artificial fertilization for different females. To study how long unfertilized eggs of Eurasian perch (Perca fluviatilis L.) can retain their fertilizing ability after stripping, eggs were stored at temperatures of 4°C, 8°C and 12°C for 72 h post-stripping (HPS). The stored eggs of four female perch were separately fertilized at 0 h (i.e. control eggs fertilized before storage) and at 6-hour intervals during the experimental period of 72 h. The embryos reaching the eyed-egg and hatched-larvae stages, eyed-egg mortality and larval malformation rates were recorded as indices of egg quality. The results indicated that the maximum eyed eggs and hatched larvae (86% and 63%, respectively) were observed for eggs fertilized immediately after stripping, whereas the storage of the eggs at 4°C for 48 HPS decreased the eyed-egg and hatched-larvae rates to 46% and 17%, respectively. The use of a higher storage temperature resulted in a more rapid decrease in egg viability: eyed-egg and hatched-larvae rates of 23% and 9%, respectively, were obtained after 48 HPS storage at 8°C and 2% and 1% for eggs stored at 12°C. Eyed-egg mortality and larval malformation rates were not significantly affected by post-stripping ova ageing for at least up to 36 h. Thereafter, both values increased significantly and were measured to be the highest in the most aged ova. The present study demonstrated that stripped Eurasian perch eggs can be stored for at least 12 h at 4°C to 12°C without a significant reduction in their quality.

Keywords

Perca fluviatilis L.strippingegg storagetemperatureegg viability
Type
Research Article
Information
animal , Volume 11 , Issue 1 , January 2017 , pp. 78 - 83
Copyright
© The Animal Consortium 2016 

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References

Aegerter, S and Jalabert, B 2004. Effects of post ovulatory oocyte ageing and temperature on egg quality and on the occurrence of triploid fry in rainbow trout Oncorhynchus mykiss . Aquaculture 231, 59–71.Google Scholar
Aegerter, S, Jalabert, B and Bobe, J 2005. Large scale real-time PCR analysis of mRNA abundance in rainbow trout eggs in relationship with egg quality and postovulatory ageing. Molecular Reproduction and Development 72, 377–385.CrossRefGoogle Scholar
Babiak, I and Dabrowski, K 2003. Refrigeration of rainbow trout gametes and embryos. Journal of Experimental Zoology Part A Comparative Experimental Biology 300A, 140–151.Google Scholar
Baras, E, Kestemont, P and Mélard, C 2003. Effect of stocking density on the dynamics of cannibalism in sibling larvae of Perca fluviatilis under controlled conditions. Aquaculture 219, 241–255.CrossRefGoogle Scholar
Bonnet, E, Jalabert, B and Bobe, J 2003. A 3-Day in vitro storage of rainbow trout Oncorhynchus mykiss unfertilised eggs in coelomic fluid at 12°C does not affect development success. Cybium 27, 47–51.Google Scholar
Craik, JCA and Harvey, SM 1984. Biochemical changes associated with overripening of the eggs of rainbow trout, Salmo gairdneri . Aquaculture 37, 347–357.Google Scholar
Fauvel, C, Suquet, M and Cosson, J 2010. Evaluation of fish sperm quality. Journal of Applied Ichthyology 26, 636–643.CrossRefGoogle Scholar
Gisbert, E and Williot, P 2002. Influence of storage duration of ovulated eggs prior to fertilization on the early ontogenesis of Sterlet, Acipenser ruthenus, and Siberian sturgeon, A. Baeri. International Review of Hydrobiology 87, 605–612.Google Scholar
Guan, M, Rawson, DM and Zhang, T 2008. Cryopreservation of zebrafish (Danio rerio) oocytes using improved controlled slow cooling protocols. Cryobiology 56 (3), 204–208.CrossRefGoogle ScholarPubMed
Kestemont, P, Jourdan, S, Houbart, M, Mélard, C, Paspatis, M, Fontaine, P, Cuvier, A, Kentouri, M and Baras, E 2003. Size heterogeneity, cannibalism and competition in cultured predatoryfish larvae: biotic and abiotic influences. Aquaculture 227, 333–356.CrossRefGoogle Scholar
Komrakova, M and Holtz, W 2009. Factors responsible for successful chilled storage of unfertilized rainbow trout (Oncorhynchus mykiss) eggs. Aquaculture 286, 156–163.CrossRefGoogle Scholar
Kucharczyk, D, Kujawa, R and Mamcarz, A 1996. New experimental incubation unit for eggs of the perch Perca fluviatilis . Progressive Fish-Culturist 58, 281–283.2.3.CO;2>CrossRefGoogle Scholar
Kucharczyk, D, Kujawa, R, Mamcarz, A, Skrzypczak, A and Wyszomirska, E 1998. Induced spawning in perch, Perca fluviatilis L., using FSH + LH with pimozide or metoclopramide. Aquaculture Research 29, 131–136.Google Scholar
Lahnsteiner, F 2000. Morphological, physiological and biochemical parameters characterizing the overripening of rainbow trout eggs. Fish Physiology and Biochemistry 23, 107–118.Google Scholar
Liang, XW, Zhu, JQ and Miao, YL 2008. Loss of methylation imprint of Snrpn in postovulatory aging mouse oocyte. Biochemical and Biophysical Research Communications 371, 16–21.Google Scholar
Linhart, O and Billard, R 1995. Survival of ovulated oocytes of the European catfish (Silurus glanis) after in vivo and in vitro storage or exposure to saline solutions and urine. Aquatic Living Resources 8, 317–322.Google Scholar
Lubzens, E, Young, G, Bobe, J and Cerda, J 2010. Oogenesis in teleosts: how fish eggs are formed. General and Comparative Endocrinology 165, 367–389.Google Scholar
Mailhes, JB, Young, D and London, SN 1998. Postovulatory ageing of mouse oocytes in vivo and premature centromere separation and aneuploidy. Biology of Reproduction 58, 1206–1210.CrossRefGoogle ScholarPubMed
Niksirat, H, Sarvi, K, Mojazi Amiri, B and Hatef, A 2007a. Effects of storage duration and storage media on initial and post-eyeing mortality of stored ova of rainbow trout Oncorhynchus mykiss . Aquaculture 262, 528–531.Google Scholar
Niksirat, H, Sarvi, K, Mojazi Amiri, B, Karami, M and Hatef, A 2007b. In vitro storage of unfertilized ova of endangered Caspian brown trout (Salmo trutta caspius) in artificial media. Animal Reproduction Science 100, 356–363.Google Scholar
Policar, T, Kouřil, J, Stejskal, V and Hamáčková, J 2008. Induced ovulation of perch (Perca fluviatilis L.) by preparations containing GnRHa with and without metoclopramide. Cybium 32 (suppl. 2), 308.Google Scholar
Rime, H, Guitton, N, Pineau, C, Bonnet, E, Bobe, J and Jalabert, B 2004. Post-ovulatory ageing and egg quality: a proteomic analysis of rainbow trout coelomic fluid. Reproductive Biology and Endocrinology 2, 26–36.CrossRefGoogle ScholarPubMed
Rizzo, E, Godinho, HP and Sato, Y 2003. Short-term storage of oocytes from the neotropical teleost fish Prochilodus marggravii . Theriogenology 60, 1059–1070.Google Scholar
Samarin, AM, Blecha, M, Uzhytchak, M, Bytyutskyy, D, Żarski, D, Flajshans, M and Policar, T 2016. Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies. Aquaculture 450, 431–438.Google Scholar
Samarin, AM, Mojazi Amiri, B, Soltani, M, Nazari, RM, Kamali, A and Naghavi, MR 2011. Effects of storage duration and storage temperature on viability of stored ova of kutum (Rutilus frisii kutum) in ovarian fluid. African Journal of Biotechnology 10, 12309–12314.Google Scholar
Samarin, AM, Policar, T and Lahnsteiner, F 2015. Fish oocyte ageing and its effect on egg quality. Reviews in Fisheries Science & Aquaculture 23, 302–314.Google Scholar
Suquet, M, Chereguini, O, Omnes, MH, Rasines, I, Normant, Y, Souto, IP and Quemener, L 1999. Effect of temperature, volume of ova batches, and addition of a diluent, an antibiotic, oxygen and a protein inhibitor on short-term storage capacities of turbot, Psetta maxima, ova. Aquatic Living Resources 12, 239–246.CrossRefGoogle Scholar
Szczerbowski, A, Kucharczyk, D, Mamcarz, A, Łuczyński, M J, Targońska, K and Kujawa, R 2009. Artificial off-season spawning of Eurasian perch Perca fluviatilis L. Archives of Polish Fisheries 17, 95–98.Google Scholar
Takahashi, T, Igarashi, H, Amita, M, Hara, S, Matsuo, K and Kurachi, H 2013. Molecular mechanism of poor embryo development in postovulatory aged oocytes: mini review. The Journal of Obstetrics and Gynaecology Research 39, 1431–1439.Google Scholar
Váarkonyi, E, Bercsényi, M, Ozouf-Costaz, C and Billard, R 1998. Chromosomal and morphological abnormalities caused by oocyte ageing in Silurus glanis . Journal of Fish Biology 52, 899–906.Google Scholar
Wakayama, S, Thuan, NV, Kishigami, S, Ohta, H, Mizutani, E, Hikichi, T, Miyake, M and Wakayama, T 2004. Production of offspring from one-day-old oocytes stored at room temperature. Journal of Reproduction and Development 50, 627–637.CrossRefGoogle ScholarPubMed
Żarski, D, Bokor, Z, Kotrik, L, Urbanyi, B, Horvath, A, Targonska, K, Krejszeff, S, Palinska, K and Kucharczyk, D 2011a. A new classification of a preovulatory oocyte maturation stage suitable for the synchronization of ovulation in controlled reproduction of Eurasian perch Perca fluviatilis L. Reproductive Biology 11, 194–209.Google Scholar
Żarski, D, Horváth, Á, Kotrik, L, Targońska, K, Palińska, K, Krejszeff, S, Bokor, Z, Urbányi, B and Kucharczyk, D 2012a. Effect of different activating solutions on the fertilization ability of Eurasian perch, Perca fluviatilis L., eggs. Journal of Applied Ichthyology 28, 967–972.CrossRefGoogle Scholar
Żarski, D, Krejszeff, S, Horváth, Á, Bokor, Z, Palińska, K, Szentes, K, Łuczyńska, J, Targońska, K, Kupren, K, Urbányi, B and Kucharczyk, D 2012b. Dynamics of composition and morphology in oocytes of Eurasian perch, Perca fluviatilis L., during induced spawning. Aquaculture 364–365, 103–110.Google Scholar
Żarski, D, Palinska, K, Targonska, K, Bokor, Z, Kotrik, L, Krejszeff, S, Kupren, K, Horvath, A, Urbanyi, B and Kucharczyk, D 2011b. Oocyte quality indicators in Eurasian perch, Perca fluviatilis L., during reproduction under controlled conditions. Aquaculture 313, 84–91.Google Scholar