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Immunological effects of altering the concentrate inclusion level in a grass silage-based diet for early lactation Holstein Friesian cows

Published online by Cambridge University Press:  01 August 2018

M. W. Little Open the ORCID record for M. W. Little [Opens in a new window] ,
A. R. G. Wylie ,
N. E. O’Connell ,
M. D. Welsh ,
C. Grelet ,
M. J. Bell ,
A. Gordon  and
C. P. Ferris
M. W. Little*
Affiliation:
Sustainable Agri-Food Sciences Division, Agri-Food and Biosciences Institute, Large Park, Hillsborough, Co. Down BT26 6DR, UK School of Biological Sciences, Institute for Global Food Security, Queens University Belfast, 18-30 Malone Road, Belfast BT9 5BN, UK
A. R. G. Wylie
Affiliation:
Sustainable Agri-Food Sciences Division, Agri-Food and Biosciences Institute, Large Park, Hillsborough, Co. Down BT26 6DR, UK
N. E. O’Connell
Affiliation:
School of Biological Sciences, Institute for Global Food Security, Queens University Belfast, 18-30 Malone Road, Belfast BT9 5BN, UK
M. D. Welsh
Affiliation:
Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stoney Road, Belfast BT4 3SD, UK
C. Grelet
Affiliation:
Valorisation of Agricultural Products Department, Walloon Agricultural Research Center, 24 Chaussée de Namur, 5030 Gembloux, Belgium
M. J. Bell
Affiliation:
School of Biosciences, The University of Nottingham, Sutton Bonington LE12 5RD, UK
A. Gordon
Affiliation:
Statistical Services Branch, Agri-Food and Biosciences Institute, 18a Newforge Lane, Belfast BT9 5PX, UK
C. P. Ferris
Affiliation:
Sustainable Agri-Food Sciences Division, Agri-Food and Biosciences Institute, Large Park, Hillsborough, Co. Down BT26 6DR, UK
*
†E-mail: [email protected]
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Abstract

Concentrate inclusion levels in dairy cow diets are often adjusted so that the milk yield responses remain economic. While changes in concentrate level on performance is well known, their impact on other biological parameters, including immune function, is less well understood. The objective of this study was to evaluate the effect of concentrate inclusion level in a grass silage-based mixed ration on immune function. Following calving 63 (45 multiparous and 18 primiparous) Holstein Friesian dairy cows were allocated to one of three isonitrogenous diets for the first 70 days of lactation. Diets comprised of a mixture of concentrates and grass silage, with concentrates comprising either a low (30%, LC), medium (50%, MC) or high (70%, HC) proportion of the diet on a dry matter (DM) basis. Daily DM intakes, milk yields and BW were recorded, along with weekly body condition score, milk composition and vaginal mucus scores. Blood biochemistry was measured using a chemistry analyzer, neutrophil phagocytic and oxidative burst assessed using commercial kits and flow cytometry, and interferon-γ production evaluated by ELISA after whole blood stimulation. Over the study period cows on HC had a higher total DM intake, milk yield, fat yield, protein yield, fat+protein yield, protein content, mean BW and mean daily energy balance, and a lower BW loss than cows on MC, whose respective values were higher than cows on LC. Cows on HC and MC had a lower serum non-esterified fatty acid concentration than cows on LC (0.37, 0.37 and 0.50 mmol/l, respectively, P=0.005, SED=0.032), while cows on HC had a lower serum β-hydroxybutyrate concentration than cows on MC and LC (0.42, 0.55 and 0.55 mmol/l, respectively, P=0.002, SED=0.03). Concentrate inclusion level had no effect on vaginal mucus scores. At week 3 postpartum, cows on HC tended to have a higher percentage of oxidative burst positive neutrophils than cows on LC (43.2% and 35.3%, respectively, P=0.078, SED=3.11), although at all other times concentrate inclusion level in the total mixed ration had no effect on neutrophil phagocytic or oxidative burst characteristics, or on interferon-γ production by pokeweed mitogen stimulated whole blood culture. This study demonstrates that for high yielding Holstein Friesian cows managed on a grass silage-based diet, concentrate inclusion levels in early lactation affects performance but has no effect on neutrophil or lymphocyte immune parameters.

Keywords

dairy cowsconcentrate levelimmune functionneutrophilsinterferon-γ
Type
Research Article
Information
animal , Volume 13 , Issue 4 , April 2019 , pp. 799 - 809
Copyright
© The Animal Consortium 2018 

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References

Allen, MS 2000. Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of Dairy Science 83, 1598–1624.Google Scholar
Andersen, JB, Friggens, NC, Sejrsen, K, Sørensen, MT, Munksgaard, L and Ingvartsen, KL 2003. The effects of low vs. high concentrate level in the diet on performance in cows milked two or three times daily in early lactation. Livestock Production Science 81, 119–128.Google Scholar
Carbonneau, E, de Passillé, AM, Rushen, J, Talbot, BG and Lacasse, P 2012. The effect of incomplete milking or nursing on milk production, blood metabolites, and immune functions of dairy cows. Journal of Dairy Science 95, 6503–6512.Google Scholar
Dann, HM and Ji, P 2013. Negative protein balance: implications for transition cows. In Proceedings of the Cornell Nutrition Conference, Cornell, NY, USA. Retrieved on 3 January 2018 from http://hdl.handle.net/1813/36477.Google Scholar
Ferris, CP, Gordon, FJ, Patterson, DC, Mayne, CS and Kilpatrick, DJ 1999. The influence of dairy cow genetic merit on the direct and residual response to level of concentrate supplementation. Journal of Agricultural Science 132, 467–481.Google Scholar
Ferris, CP, Gordon, FJ, Patterson, DC, Mayne, CS and McCoy, MA 2003. A short-term comparison of the performance of four grassland-based systems of milk production for autumn-calving dairy cows. Grass and Forage Science 58, 192–209.Google Scholar
Galvão, KN, Flaminio, MJBF, Brittin, SB, Sper, R, Fraga, M, Caixeta, L, Ricci, A, Guard, CL, Butler, WR and Gilbert, RO 2010. Association between uterine disease and indicators of neutrophil and systemic energy status in lactating Holstein cows. Journal of Dairy Science 93, 2926–2937.Google Scholar
Gilbert, RO, Gröhn, YT, Miller, PM and Hoffman, DJ 1993. Effect of parity on periparturient neutrophil function in dairy cows. Veterinary Immunology and Immunopathology 36, 75–82.Google Scholar
Grummer, RR, Mashek, DG and Hayirli, A 2004. Dry matter intake and energy balance in the transition period. Veterinary Clinics of North American Food Animal Practice 20, 447–470.Google Scholar
Heiser, A, McCarthy, A, Wedlock, N, Meier, S, Kay, J, Walker, C, Crookenden, MA, Mitchell, MD, Morgan, S, Watkins, K, Loor, JJ and Roche, JR 2015. Grazing dairy cows had decreased interferon-gamma, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving. Journal of Dairy Science 98, 937–946.Google Scholar
Houdijk, JG, Kyriazakis, I, Jackson, F, Huntley, JF and Coop, RL 2005. Effects of protein supply and reproductive status on local and systemic immune responses to Teladorsagia circumcincta in sheep. Veterinary Parasitology 129, 105–117.Google Scholar
Ingvartsen, KL 2006. Feeding- and management-related diseases in the transition cow: physiological adaptations around calving and strategies to reduce feeding-related diseases. Animal Feed Science and Technology 126, 175–213.Google Scholar
Ingvartsen, KL and Moyes, K 2013. Nutrition, immune function and health of dairy cattle. Animal 7, 112–122.Google Scholar
Jenkins, TC and McGuire, MA 2006. Major advances in nutrition: impact on milk composition. Journal of Dairy Science 89, 1302–1310.Google Scholar
Jones, LA, Houdijk, JGM, Sakkas, P, Bruce, AD, Mitchell, M, Knox, DP and Kyriazakis, I 2011. Dissecting the impact of protein versus energy host nutrition on the expression of immunity to gastrointestinal parasites during lactation. International Journal of Parasitology 41, 711–719.Google Scholar
Kimura, K, Goff, JP and Kehrli, ME Jr 1999. Effects of the presence of the mammary gland on expression of neutrophil adhesion molecules and myeloperoxidase activity in periparturient dairy cows. Journal of Dairy Science 82, 2385–2392.Google Scholar
Lacetera, N, Scalia, D, Bernabucci, U, Ronchi, B, Pirazzi, D and Nardone, A 2005. Lymphocyte functions in overconditioned cows around parturition. Journal of Dairy Science 88, 2010–2016.Google Scholar
Lessard, M, Gagnon, N, Godson, DL and Petit, HV 2004. Influence of parturition and diets enriched in n-3 or n-6 polyunsaturated fatty acids on immune response of dairy cows during the transition period. Journal of Dairy Science 87, 2197–2210.Google Scholar
Li, P, Yin, YL, Li, D, Kim, SW and Wu, G 2007. Amino acids and immune function. The British Journal of Nutrition 98, 237–252.Google Scholar
Little, MW, O’Connell, NE, Welsh, MD, Barley, J, Meade, KG and Ferris, CP 2016. Prepartum concentrate supplementation of a medium quality grass silage based diet: effects on performance, health, fertility, metabolic function and immune function of low body condition score cows. Journal of Dairy Science 99, 7102–7122.Google Scholar
Little, MW, O’Connell, NE, Welsh, MD, Mulligan, FJ and Ferris, CP 2017. Concentrate supplementation of a medium quality grass-silage based diet for 4 weeks prepartum: effects on cow performance, health, metabolic status, and immune function. Journal of Dairy Science 100, 4457–4474.Google Scholar
Llamas Moya, S, Alonso Gómez, M, Boyle, LA, Mee, JF, O’Brien, B and Arkins, S 2008. Effects of milking frequency on phagocytosis and oxidative burst activity of phagocytes from primiparous and multiparous dairy cows during early lactation. Journal of Dairy Science 91, 587–595.Google Scholar
Loiselle, MC, Ster, C, Talbot, BG, Zhao, X, Wagner, GF, Boisclair, YR and Lacasse, P 2009. Impact of postpartum milking frequency on the immune system and the blood metabolite concentration of dairy cows. Journal of Dairy Science 92, 1900–1912.Google Scholar
Machado, SC, McManus, CM, Stumpf, MT and Fischer, V 2014. Concentrate: forage ratio in the diet of dairy cows does not alter milk physical attributes. Tropical Animal Health and Production 46, 855–859.Google Scholar
McCarthy, MM, Yasui, T, Ryan, CM, Mechor, GD and Overton, TR 2015a. Performance of early-lactation dairy cows as affected by dietary starch and monensin supplementation. Journal of Dairy Science 98, 3335–3350.Google Scholar
McCarthy, MM, Yasui, T, Ryan, CM, Pelton, SH, Mechor, GD and Overton, TR 2015b. Metabolism of early-lactation dairy cows as affected by dietary starch and monensin supplementation. Journal of Dairy Science 98, 3351–3365.Google Scholar
Mulligan, FJ and Doherty, ML 2008. Production diseases of the transition cow. The Veterinary Journal 176, 3–9.Google Scholar
Newsholme, P, Curi, R, Gordon, S and Newsholme, EA 1986. Metabolism of glucose, glutamine, long-chain fatty acids and ketone bodies by murine macrophages. Biochemical Journal 239, 121–125.Google Scholar
Nonnecke, BJ, Kimura, K, Goff, JP and Kehrli, ME Jr 2003. Effects of the mammary gland on functional capacities of blood mononuclear leukocyte populations from periparturient cows. Journal of Dairy Science 86, 2359–2368.Google Scholar
O’Driscoll, K, Olmos, G, Llamas Moya, S, Mee, JF, Earley, B, Gleeson, D, O’Brien, B and Boyle, L 2012. A reduction in milking frequency and feed allowance improves dairy cow immune status. Journal of Dairy Science 95, 1177–1187.Google Scholar
Paape, M, Mehrzad, J, Zhao, X, Detilleux, J and Burvenich, C 2002. Defense of the bovine mammary gland by polymorphonuclear neutrophil leukocytes. Journal of Mammary Gland Biology and Neoplasia 7, 109–121.Google Scholar
Rabelo, E, Rezende, RL, Bertics, SJ and Grummer, RR 2003. Effects of transition diets varying in dietary energy density on lactation performance and ruminal parameters of dairy cows. Journal of Dairy Science 86, 916–925.Google Scholar
Radostits, OM, Gay, CC, Hinchcliff, KW and Constable, PD (ed.) 2007. Appendix 2, reference laboratory values. In Veterinary medicine: a textbook of the diseases of cattle, sheep, pigs, goats and horses 10th edition, pp. 2047–2051. Elsevier Saunders, Edinburgh, UK.Google Scholar
Roche, JR, Bell, AW, Overton, TR and Loor, JJ 2013. Nutritional management of the transition cow in the 21st century – a paradigm shift in thinking. Animal Reproduction Science 53, 1000–1023.Google Scholar
Scalia, D, Lacetera, N, Bernabucci, U, Demeyere, K, Duchateau, L and Burvenich, C 2006. In vitro effects of nonesterified fatty acids on bovine neutrophils oxidative burst and viability. Journal of Dairy Science 89, 147–154.Google Scholar
Schroder, K, Hertzog, PJ, Ravasi, T and Hume, DA 2004. Interferon-γ: an overview of signals, mechanisms and functions. Journal of Leukocyte Biology 75, 163–189.Google Scholar
Sheldon, IM, Cronin, J, Goetze, L, Donofrio, G and Schuberth, HJ 2009. Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biology of Reproduction 81, 1025–1032.Google Scholar
Sordillo, LM 2016. Nutritional strategies to optimize dairy cattle immunity. Journal of Dairy Science 99, 4967–4982.Google Scholar
Ster, C, Loiselle, MC and Lacasse, P 2012. Effect of postcalving serum nonesterified fatty acids concentration on the functionality of bovine immune cells. Journal of Dairy Science 95, 708–717.Google Scholar
Sterk, A, Johansson, BE, Taweel, HZ, Murphy, M, van Vuuren, AM, Hendriks, WH and Dijkstra, J 2011. Effects of forage type, forage to concentrate ratio, and crushed linseed supplementation on milk fatty acid profile in lactating dairy cows. Journal of Dairy Science 94, 6078–6091.Google Scholar
Suriyasathaporn, W, Heuer, C, Noordhuizen-Stassen, EN and Schukken, YH 2000. Hyperketonemia and the impairment of udder defense: a review. Veterinary Research 31, 397–412.Google Scholar
Yasui, T, McCarthy, MM, Ryan, CM, Gilbert, RO, Felippe, MJB, Mechor, GD and Overton, TR 2016. Effects of monensin and starch level in early lactation diets on indices of immune function in dairy cows. Journal of Dairy Science 99, 1351–1363.Google Scholar