Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-15T07:28:13.176Z Has data issue: false hasContentIssue false

Grazing increases the concentration of CLA in dairy cow milka

Published online by Cambridge University Press:  30 April 2014

M. N. Lahlou
Affiliation:
US Dairy Forage Research Center, USDA-ARS, Madison, WI 53706, USA
R. Kanneganti
Affiliation:
US Dairy Forage Research Center, USDA-ARS, Madison, WI 53706, USA
L. J. Massingill
Affiliation:
US Dairy Forage Research Center, USDA-ARS, Madison, WI 53706, USA
G. A. Broderick
Affiliation:
US Dairy Forage Research Center, USDA-ARS, Madison, WI 53706, USA
Y. Park
Affiliation:
Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA
M. W. Pariza
Affiliation:
Food Research Institute, University of Wisconsin, Madison, WI 53706, USA
J. D. Ferguson
Affiliation:
New Bolton Center, University of Pennsylvania, Kennett Square, PA 19348, USA
Z. Wu*
Affiliation:
New Bolton Center, University of Pennsylvania, Kennett Square, PA 19348, USA
*
Get access

Abstract

An experiment was conducted to examine whether increased CLA in milk of dairy cows fed fresh pasture compared with alfalfa and corn silages was because of ruminal or endogenous synthesis. Eight Holsteins were fed a total mixed ration using alfalfa and corn silages as the forage source in confinement or grazed in a replicated crossover design. The proportion of total fatty acids as CLA (primarily c9, t11-18:2) in g/100 g was 0.44 v. 0.28 in ruminal digesta, 0.89 v. 0.53 in omasal digesta and 0.71 v. 1.06 in milk during confinement feeding and grazing, respectively. Blood plasma CLA was 0.54 v. 1.05 mg/l for the two treatments, respectively. The increased concentration of CLA in milk with grazing likely resulted from increased synthesis through desaturation of t11-18:1 in the mammary gland.

Type
Full Paper
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Broderick, GA and Kang, JH 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science 63, 6475.Google Scholar
Brotz, PG and Schaefer, DM 1987. Simultaneous determination of lactic acid and volatile fatty acids in microbial fermentation extracts by gas-liquid chromatography. Journal of Microbiological Methods 6, 139144.Google Scholar
Cabrita, ARJ, Bessa, RJB, Alves, SP, Dewhurst, RJ and Fonseca, AJM 2007. Effects of dietary protein and starch on intake, milk production, and milk fatty acid profiles of dairy cows fed corn silage-based diets. Journal of Dairy Science 90, 14291439.Google Scholar
Chin, SF, Liu, W, Storkson, JM, Ha, YL and Pariza, MW 1992. Dietary sources of conjugated linoleic acid, a newly recognized class of anticarcinogens. Journal of Food Composition and Analysis 5, 185197.CrossRefGoogle Scholar
Christie, WW 1979. The effect of diet and other factors on the lipid composition of ruminant tissues and milk. Progress in Lipid Research 17, 245277.Google Scholar
Corl, BA, Baumgard, LH, Dwyer, DA, Griinari, JM, Philipis, BS and Bauman, DE 2001. The role of Δ9-desaturase in the production of cis-9, trans-11 CLA. The Journal of Nutritional Biochemistry 12, 661666.Google Scholar
Dawson, RMC and Kemp, P 1970. Biohydrogenation of dietary fats in ruminants. In Physiology of digestion and metabolism in the ruminant (ed. AT Phillipson), pp. 504518. Oriel Press, Newcastle-upon-Tyne, England.Google Scholar
Destaillats, F, Trottier, JP, Galvez, JMG and Angers, P 2005. Analysis of α-linolenic acid biohydrogenation intermediates in milk fat with emphasis on conjugated linolenic acids. Journal of Dairy Science 88, 32313239.CrossRefGoogle ScholarPubMed
Dhiman, TR, Anada, GR, Satter, LD and Pariza, MW 1999. Conjugated linoleic acid content from cows fed different diets. Journal of Dairy Science 82, 21462156.Google Scholar
Griinari, JM, Dwyer, DA, McGuire, MA, Bauman, DE, Palmquist, DL and Nurmela, KVV 1998. Trans-octadecenoic acids and milk fat depression in lactating dairy cows. Journal of Dairy Science 81, 12511261.Google Scholar
Griinari, JM, Corl, BA, Lacy, SH, Chouinard, PY, Nurmela, KVV and Bauman, DE 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Δ9-desaturase. Journal of Nutrition 130, 22852291.Google Scholar
Halmemies-Beauchet-Filleau, A, Kairenius, P, Ahvenjärvi, S, Crosley, LK, Muetzel, S, Huhtanen, P, Vanhatalo, A, Toivonen, V, Wallace, RJ and Shingfield, KJ 2013a. Effect of forage conservation method on ruminal lipid metabolism and microbial ecology in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. Journal of Dairy Science 96, 24282447.Google Scholar
Halmemies-Beauchet-Filleau, A, Kairenius, P, Ahvenjärvi, S, Toivonen, V, Huhtanen, P, Vanhatalo, A, Givens, DI and Shingfield, KJ 2013b. Effect of forage conservation method on plasma lipids, mammary lipogenesis, and milk fatty acid composition in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. Journal of Dairy Science 96, 52675289.CrossRefGoogle Scholar
Halmemies-Beauchet-Filleau, A, Vanhatalo, A, Toivonen, V, Heikkilä, T, Lee, MRF and Shingfield, KJ 2013c. Effect of replacing grass silage with red clover silage on ruminal lipid metabolism in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. Journal of Dairy Science 96, 58825900.CrossRefGoogle Scholar
Jenkins, TC, Wallace, RJ, Moate, PJ and Mosley, EE 2008. Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Journal of Animal Science 86, 397412.Google Scholar
Kay, JK, Mackle, TR, Auldist, MJ, Thompson, NA and Bauman, DE 2004. Endogenous synthesis of cis-9, trans-11 conjugated linoleic acid in dairy cows fed fresh pasture. Journal of Dairy Science 87, 369378.Google Scholar
Kelly, ML, Kolver, ES, Bauman, DE, VanAmburgh, ME and Muller, LD 1998. Effect of intake of pasture on concentration of conjugated linoleic acid in milk of lactating cows. Journal of Dairy Science 81, 16301636.Google Scholar
Kemp, P, White, RW and Lander, DJ 1975. Hydrogenation of unsaturated fatty acids by five bacterial isolates from sheep rumen, including a new species. Journal of General Microbiology 90, 100114.Google Scholar
Kepler, CR, Hirons, KP, McNeill, JJ and Tove, SB 1966. Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens . The Journal of Biological Chemistry 241, 13501354.Google Scholar
Kim, EJ, Huws, SA, Lee, MRF and Scollan, ND 2009. Dietary transformation of lipid in the rumen microbial ecosystem. Asia-Australian Journal of Animal Science 22, 13411350.CrossRefGoogle Scholar
Kim, YJ, Liu, RH, Rychlik, JL and Russell, JB 2002. The enrichment of a ruminal bacterium (Megasphaera elsdenii YJ-4) that produces the trans-10, cis-12 isomer of conjugated linoleic acid. Journal of Applied Microbiology 92, 976982.Google Scholar
Lee, MRF, Theobald, VJ, Tweed, JKS, Winters, AL and Scollan, ND 2009. Effect of feeding fresh or conditioned red clover on milk fatty acids and nitrogen utilization in lactating dairy cows. Journal of Dairy Science 92, 11361147.CrossRefGoogle ScholarPubMed
Loor, JJ, Herbein, JH and Polan, CE 2002. Trans 18:1 and 18:2 isomers in blood plasma and milk fat of grazing cows fed a grain supplement containing solvent-extracted or mechanically extracted soybean meal. Journal of Dairy Science 85, 11971207.Google Scholar
Mohammed, R, Stanton, CS, Kennelly, JJ, Kramer, JKG, Mee, JF, Glimm, DR, O’Donovan, M and Murphy, JJ 2009. Grazing cows are more efficient than zero-grazed and grass silage-fed cows in milk rumenic acid production. Journal of Dairy Science 92, 38743893.Google Scholar
Moore, JH and Christie, WW 1979. Lipid metabolism in the mammary gland of ruminant animals. Progress in Lipid Research 17, 347395.Google Scholar
Morales, MS, Palmquist, DL and Weiss, WP 2000. Effects of fat source and copper on unsaturation of blood and milk triacylglycerol fatty acids in Holstein and Jersey cows. Journal of Dairy Science 83, 21052111.CrossRefGoogle ScholarPubMed
Mosley, EE, Shafii, B, Moate, PJ and McGuire, MA 2006. Cis-9, trans-11 conjugated linoleic acid is synthesized directly from vaccenic acid in lactating dairy cattle. Journal of Nutrition 136, 570575.CrossRefGoogle ScholarPubMed
Noble, RC, Moore, JH and Harfoot, CG 1974. Observations on the pattern on biohydrogenation of esterified and unesterified linoleic acid in the rumen. British Journal of Nutrition 31, 99108.Google Scholar
NRC 2001. Nutrient requirements of dairy cattle, 7th edition. National Academy Press, Washington, DC, USA.Google Scholar
Palmquist, DL 1988. The feeding value of fats. In Feed science (ed. ER Orskov), World Animal Science B4, pp. 293311. Elsevier Science Publishers, Amsterdam, The Netherlands.Google Scholar
Palmquist, DL, Lock, AL, Shingfield, KJ and Bauman, DE 2005. Biosynthesis of conjugated linoleic acid in ruminants and humans. Advances in Food Nutrition Research 50, 179217.Google Scholar
Perfield, JW II, Bernal-Santos, G, Overton, TR and Bauman, DE 2002. Effects of dietary supplementation of rumen-protected conjugated linoleic acid (CLA) in dairy cows during established lactation. Journal of Dairy Science 85, 26092617.CrossRefGoogle ScholarPubMed
Piperova, LS, Sampugna, J, Teter, BB, Kalscheur, KF, Yurawecz, MP, Ku, Y, Morehouse, KM and Erdman, RA 2002. Duodenal and milk trans octadecenoic acid and conjugated linoleic acid (CLA) isomers indicate that postabsorptive synthesis is the predominant source of cis-9-containing CLA in lactating dairy cows. Journal of Nutrition 132, 12351241.Google Scholar
Polan, CE, McNell, JJ and Tove, SB 1964. Biohydrogenation of unsaturated fatty acids by rumen bacteria. Journal of Bacteriology 88, 10561064.Google Scholar
Robertson, JB and Van Soest, PJ 1981. The detergent system of analysis. In The analysis of dietary fibre in food (ed. WPT Kames and O Theander), Chapter 9, pp. 123158. Marcel Dekker Inc., New York, NY, USA.Google Scholar
SAS 2000. SAS® user’s guide, statistics, version 8. SAS Institute, Inc., Cary, NC, USA.Google Scholar
Schroeder, GF, Delahoy, JE, Vidaurreta, I, Bargo, F, Gagliostro, GA and Muller, LD 2003. Milk fatty acid composition of cows fed a total mixed ration or pasture plus concentrates replacing corn with fat. Journal of Dairy Science 86, 32373248.CrossRefGoogle ScholarPubMed
Solomon, R, Chase, LE, Ben-Ghedalia, D and Bauman, DE 2000. The effect of nonstructural carbohydrate and addition of full fat extruded soybeans on the concentration of conjugated linoleic acid in the milk fat of dairy cows. Journal of Dairy Science 83, 13221329.Google Scholar
Timmen, H and Patton, S 1988. Milk fat globules: fatty acid composition, size and in vivo regulation of fat liquidity. Lipids 23, 685689.Google Scholar
Toral, PG, Bernard, L, Chilliard, Y and Glasser, F 2013. Short communication: diet-induced variations in milk fatty acid composition have minor effects on the estimated melting point of milk fat in cows, goats, and ewes: insights from a meta-analysis. Journal of Dairy Science 96, 12321236.Google Scholar
Wu, Z, Kanneganti, VR, Massingill, LJ, Wiltbank, MC, Walgenbach, RP and Satter, LD 2001. Milk production of fall-calving dairy cows during summer grazing of grass pasture or grass-clover pastures. Journal of Dairy Science 84, 11661173.Google Scholar
Yang, A, Larsen, TW, Smith, SB and Tume, RK 1999. Δ-9 desaturase activity in bovine subcutaneous adipose tissue of different fatty acid composition. Lipids 34, 971997.Google Scholar