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Gene expression, enzyme activity and performance of Nile tilapia larvae fed with diets of different CP levels

Published online by Cambridge University Press:  03 December 2018

W. S. Silva ,
L. S. Costa ,
J. F. López-Olmeda ,
N. C. S. Costa ,
W. M. Santos ,
P. A. P. Ribeiro  and
R. K. Luz Open the ORCID record for R. K. Luz [Opens in a new window]
W. S. Silva
Affiliation:
Departamento de Zootecnia, Universidade Federal de Minas Gerais, Escola de Veterinária, Laboratório de Aquacultura, Avenida Antônio Carlos, n° 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brasil
L. S. Costa
Affiliation:
Departamento de Zootecnia, Universidade Federal de Minas Gerais, Escola de Veterinária, Laboratório de Aquacultura, Avenida Antônio Carlos, n° 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brasil
J. F. López-Olmeda
Affiliation:
Departamento de Fisiología, Universidad de Murcia, Facultad de Biología, Avda. Teniente Flomesta, n° 5, CEP 30003 Murcia, España
N. C. S. Costa
Affiliation:
Departamento de Zootecnia, Universidade Federal de Lavras, Laboratório de Enzimologia, Av. Sul UFLA - Aquenta Sol, CEP 37200-000 Lavras, Minas Gerais, Brasil
W. M. Santos
Affiliation:
Departamento de Zootecnia, Universidade Federal de Minas Gerais, Escola de Veterinária, Laboratório de Aquacultura, Avenida Antônio Carlos, n° 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brasil
P. A. P. Ribeiro
Affiliation:
Departamento de Zootecnia, Universidade Federal de Minas Gerais, Escola de Veterinária, Laboratório de Aquacultura, Avenida Antônio Carlos, n° 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brasil
R. K. Luz*
Affiliation:
Departamento de Zootecnia, Universidade Federal de Minas Gerais, Escola de Veterinária, Laboratório de Aquacultura, Avenida Antônio Carlos, n° 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brasil
*
†E-mail: [email protected]
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Abstract

Protein is the most costly nutrient in fish feed, and while diets offered in the early stages of development typically have high levels of CP, they do not always correspond to the real requirements of the animals. Thus, research that seeks to learn the true nutritional requirements of fish is fundamental to improving commercial fish culture. The present study evaluated the protein requirements of Nile tilapia (Oreochromis niloticus) under larviculture. Fish performance, gene expression for digestive enzymes and their enzymatic activity and stress response to air exposure were analyzed. Four experimental diets differing in CP level were formulated: 30%, 36%, 42% and 48%. Fish larvae were fed the experimental diets during development and sampled 10, 20 and 30 days after the beginning of the experiment for performance, gene expression and enzymatic activity. At sampling time 30, stress resistance was also evaluated by means of an air exposure test. At sampling time 10, CP levels between 36% and 48% could be used for a better performance. During this period, pepsinogen expression was greater for 30% CP, intermediate for 42% and lower for 36% and 48%. After this initial period, diets of between 30% and 42% CP are recommended for better performance. At sampling time 20, gene expression for digestive enzymes and their enzymatic activity were similar for all diets tested. At sampling time 30, the diet of 42% CP induced both greater pepsinogen expression and pepsin activity. Survival after the air exposure test after 30 days of feeding was influenced by CP level in the diet, with the highest survival being for fish fed with 36% CP. Taken together, the present results demonstrate that dietary CP influences digestive enzyme gene expression and activity, and suggest that the best CP levels for Nile tilapia larviculture vary depending on larval stage.

Keywords

oreochromis niloticuslarvicultureenzymologydigestionstress
Type
Research Article
Information
animal , Volume 13 , Issue 7 , July 2019 , pp. 1376 - 1384
Copyright
© The Animal Consortium 2018 

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References

Abdel-Tawwab, M, Ahmad, MH, Sakr, SFM and Seden, MEA 2010. Use of green tea, Camellia sinensis L. in practical diet for growth and protection of Nile tilapia, Oreochromis niloticus (L.) against Aeromonas hydrophila infection. Journal of World Aquaculture Society 41, 203–213.Google Scholar
Al Hafedh, YS 1999. Effects of dietary protein on growth and body composition of Nile tilapia, Oreochromis niloticus L. Aquaculture Research 30, 385–393.Google Scholar
Association of Official Analytical Chemists 2012. Official methods of analysis, 19th edition. AOAC, Arlington, VA, USA.Google Scholar
Boscolo, WR, Hayashi, C and Meurer, F 2002. Apparent digestibility of the energy and nutrients of conventional and alternatives foods for Nile tilapia (Oreochromis niloticus). Revista Brasileira de Zootecnia 31, 539–545.Google Scholar
Bradford, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Cara, B, Moyano, FJ, Zambonino-Infante, JL and Fauvel, C 2007. Trypsin and chymotrypsin as indicators of nutritional status of post-weaned sea bass larvae. Journal of Fish Biology 70, 1798–1808.Google Scholar
Costa, LS, Serrano, I, Sánchez-Vázquez, FJ and López-Olmeda, JF 2016. Circadian rhythms of clock gene expression in Nile tilapia (Oreochromis niloticus) central and peripheral tissues: influence of different lighting and feeding conditions. Journal of Comparative Physiology B 186, 775–785.Google Scholar
Darias, MJ, Murray, HM, Martínez-Rodríguez, G, Cárdenas, S and Yúfera, M 2005. Gene expression of pepsinogen during the larval development of red porgy (Pagrus pagrus). Aquaculture 248, 245–252.Google Scholar
Diógenes, AF, Fernandes, JBK, Dorigam, JCP, Sakomura, NK, Rodrigues, FHF, Lima, BTM and Gonçalves, FH 2016. Establishing the optimal essential amino acids ratios in juveniles of Nile tilapia (Oreochromis niloticus) by the deletion method. Aquaculture Nutrition 22, 435–443.Google Scholar
Douglas, SE, Gawlicka, A, Mandla, S and Gallant, JW 1999. Ontogeny of the stomach in winter flounder: characterization and expression of the pepsinogen and proton pump genes and determination of pepsin activity. Journal of Fish Biology 55, 897–915.Google Scholar
El-Sayed, AFM 1999. Alternative dietary protein sources for farmed tilápia Oreochromis spp. Aquaculture 179, 149–168.Google Scholar
Galaviz, MA, García-Gasca, A, Drawbridge, M, Álvarez-González, CA and López, LM 2011. Ontogeny of the digestive tract and enzymatic activity in white seabass, Atractoscion nobilis, larvae. Aquaculture 318, 162–168.Google Scholar
Galvão, MSN, Yamanaka, N, Fenerich-Verani, N and Pimentel, CMM 1997. Estudos preliminares sobre enzimas digestivas proteolíticas da tainha Mugil platanus Gunter, 1980 (Osteichthyes, Mugilidae) durante as fases larval e juvenil. Boletim do Instituto de Pesca 24, 101–110.Google Scholar
Huang, L, Schreiber, AM, Soffientino, B, Bengtson, DA and Specker, JL 1998. Metamorphosis of summer flounder (Paralichthys dentatus): thyroid status and the timing of gastric gland formation. Journal of Experimental Zoology 280, 413–420.Google Scholar
Jones, DA, Kumlu, M, Le Vay, L and Fletcher, DJ 1997. The digestive physiology of herbivorous, omnivorous and carnivorous crustacean larvae: a review. Aquaculture 155, 289–299.Google Scholar
Lo, M and Weng, CF 2006. Developmental regulation of gastric pepsin and pancreatic serine protease in larvae of the euryhaline teleost, Oreochromis mossambicus . Aquaculture 261, 1403–1412.Google Scholar
Luz, RK and Portella, MC 2005. Tolerance to the air exposition test of Hoplias lacerdae larvae and juvenile during its initial development. Brazilian Archives of Biology and Technology 48, 567–573.Google Scholar
Luz, RK, Ribeiro, PAP, Ikeda, AL, Santos, AEH, Melillo Filho, R, Turra, EM and Teixeira, EA 2012. Performance and stress resistance of Nile tilapias fed different crude protein levels. Revista Brasileira de Zootecnia 41, 457–461.Google Scholar
Montoya, A, López-Olmeda, JF, Yúfera, M, Sánchez-Muros, MJ and Sánchez-Vázquez, FJ 2010. Feeding time synchronises daily rhythms of behaviour and digestive physiology in gilthead seabream (Sparus aurata). Aquaculture 306, 315–321.Google Scholar
Morais, S, Conceição, LEC, Rǿnnestad, I, Koven, W, Chantal, C, Zambonino, JL and Dinis, M 2007. Dietary neutral lipid level and source in marine fish larvae: effects on digestive physiology and food intake. Aquaculture 268, 106–122.Google Scholar
Murray, HM, Gallanta, JW, Johnsona, SC and Douglas, SE 2006. Cloning and expression analysis of three digestive enzymes from Atlantic halibut (Hippoglossus hippoglossus) during early development: predicting gastrointestinal functionality. Aquaculture 252, 394–408.Google Scholar
Murashita, K, Fukada, H, Takahashi, N, Hosomi, N, Matsunari, H, Furuita, H, Oku, H and Yamamoto, T 2015. Effect of feed ingredients on digestive enzyme secretion in fish. Bulletin of Fisheries Research Agency 40, 69–74.Google Scholar
Nunes, ESS, Cavero, BAS and Pereira-Filho, M 2006. Enzimas digestivas exógenas na alimentação de juvenis de tambaqui. Pesquisa Agropecuária Brasileira 41, 139–143.Google Scholar
Ozorio, ROA, Valente, LMP, Pousão-Ferreira, P and Oliva-Teles, A 2006. Growth performance and body composition of white seabream (Diplodus sargus) juveniles fed diets with different protein and lipid levels. Aquaculture Research 37, 255–263.Google Scholar
Piccinetti, CC, Grasso, L, Maradonna, F, Radaelli, G, Ballarin, C, Chemello, G, Evjemo, JO, Carnevali, O and Olivotto, I 2017. Growth and stress factors in ballan wrasse (Labrus bergylta) larval development. Aquaculture Research 48, 2567–2580.Google Scholar
Rǿnnestad, I, Yúfera, M, Ueberschär, B, Ribeiro, L, Sæle, Ø and Boglione, C 2013. Feeding behaviour and digestive physiology in larval fish: current knowledge, and gaps and bottlenecks in research. Reviews in Aquaculture 5, 59–98.Google Scholar
Santos, JCE and Luz, RK 2009. Effect of salinity and prey concentrations on Pseudoplatystoma corruscans, Prochilodus costatus and Lophiosilurus alexandri larviculture. Aquaculture 287, 324–328.Google Scholar
Stiassny, MLJ 1991. Phylogenetic intrarelationships of the family Cichlidae: an overview. In Cichlid fishes. Behaviour, ecology and evolution (ed. MHA Keenleyside), pp 1–35. Chapman and Hall, London, UK.Google Scholar
Tacon, AGJ, Food and Agriculture Organization of the United Nations 1990. Standard methods for the nutrition and feeding of farmed fish and shrimp. Argent Laboratories Press, Redmond, Washington, DC, USA.Google Scholar
Tengjaroenkul, B, Smith, BJ, Smith, SA and Chatreewongsin, U 2002. Ontogenic development of the intestinal enzymes of cultured Nile tilapia, Oreochromis niloticus L. Aquaculture 211, 241–251.Google Scholar
Terova, G, Rimoldi, S, Larghi, S, Bernardini, G, Gornati, R and Saroglia, M 2007. Regulation of progastricsin mRNA levels in sea bass (Dicentrarchus labrax) in response to fluctuations in food availability. Biochemical and Biophysical Research Communications 363, 591–596.Google Scholar
Trushenski, J, Schwarz, M, Takeuchi, R, Delbos, B and Sampaio, LA 2010. Physiological responses of cobia Rachycentron canadum following exposure to low water and air exposure stress challenges. Aquaculture 307, 173–177.Google Scholar
Untergasser, A, Cutcutache, I, Koressaar, T, Ye, J, Faircloth, BC, Remm, M and Rozen, SG 2012. Primer3-new capabilities and interfaces. Nucleic Acids Research 40, 1–12.Google Scholar
Volkoff, H, Unniappan, S and Kelly, SP 2009. The endocrine regulation of food intake. Fish Neuroendocrinology 28, 421–465.Google Scholar
Wang, C, Shouqi, X, Xiaoming, Z, Leia, W, Yanga, Y and Liua, J 2006. Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae. Aquaculture 254, 554–562.Google Scholar
Yang, CG, Wang, XL, Tian, J, Liu, W, Wu, F, Jiang, M and Wen, H 2013. Evaluation of reference genes for quantitative real-time RTPCR analysis of gene expression in Nile tilapia (Oreochromis niloticus). Gene 527, 183–192.Google Scholar