Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-18T16:45:37.312Z Has data issue: false hasContentIssue false
  • English
  • Français

First cloned swamp buffalo produced from adult ear fibroblast cell

Published online by Cambridge University Press:  07 May 2014

K. Tasripoo ,
W. Suthikrai ,
S. Sophon ,
R. Jintana ,
W. Nualchuen ,
S. Usawang ,
A. Bintvihok ,
M. Techakumphu  and
K. Srisakwattana
K. Tasripoo
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
W. Suthikrai
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
S. Sophon
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
R. Jintana
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
W. Nualchuen
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
S. Usawang
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
A. Bintvihok
Affiliation:
Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
M. Techakumphu
Affiliation:
Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
K. Srisakwattana*
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
*
†E-mail: [email protected]
Get access

Abstract

The world’s first cloned swamp buffalo (Bubalus bubalis) derived from adult ear skin fibroblast has been reported. Donor fibroblast cells were produced from biopsies taken from adult male ear skin and in vitro matured oocytes obtained from a slaughterhouse were used as cytoplasts. A total of 39 blastocysts and 19 morulae fresh embryos were transferred into 12 recipient buffaloes. Progesterone assays indicated establishment of pregnancy in 10 of the 12 buffaloes (83.3%) after 45 days, with six animals still pregnant at 3 months. One recipient maintained pregnancy to term and naturally delivered a 40 kg male calf after 326 days of gestation. DNA analysis showed that the cloned calf was genetically identical to the donor cells. Genotype analyses, using 12 buffalo microsatellite markers, confirmed that the cloned calf was derived from the donor cell lines. In conclusion, the present study reports, for the first time, the establishment of pregnancy and birth of the first cloned Thai swamp buffalo derived from adult ear skin fibroblast cells.

Keywords

cloningear skin fibroblastsomatic cell nuclear transferswamp buffalo
Type
Full Paper
Information
animal , Volume 8 , Issue 7 , July 2014 , pp. 1139 - 1145
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chaikhun, T, Hengtrakunsin, R, De Rensis, F, Techakumphu, M and Suadsong, S 2012. Reproductive and dairy performance of Thai swamp buffaloes under intensive farm management. The Thai Journal of Veterinary Medicine 42 (1), 81–85.Google Scholar
Chavatte-Palmer, P, Camous, S, Jammes, H, Le Cleac’h, N, Guillomot, M and Lee, RSF 2012. Review: placental perturbation induce the developmental abnormalities often observed in bovine somatic cell nuclear transfer. Placenta 26, s99–s104.CrossRefGoogle Scholar
Dobson, H and Kamonpatana, M 1986. A review of female cattle reproduction with special reference to a comparison between buffaloes, cows and zebu. Journal of Reproduction and Fertility 77, 1–36.Google Scholar
Forsberg, EJ, Strelchenko, NS, Augenstein, ML, Bethauser, JM, Childs, LA, Eilertsen, KJ, Enos, JM, Forsythe, TM, Golueke, PJ, Koppang, RW, Lange, G, Lesmeister, TL, Mallon, KS, Mell, GD, Misica, PM, Pace, MM, Pfister-Genskow, M, Voelker, GR, Watt, SR and Bishop, MD 2002. Production of cloned cattle from in vitro systems. Biology of Reproduction 67, 327–333.Google Scholar
Heyman, Y, Chavatte-Palmer, P, LeBourhis, D, Camous, S, Vignon, X and Renard, JP 2002. Frequency and occurrence of late-gestation losses from cattle cloned embryos. Biology of Reproduction 66, 6–13.Google Scholar
Hill, JR, Burghardt, RC, Jones, K, Long, CR, Looney, CR, Shin, T, Spencer, TE, Thompson, JA, Winger, QA and Westhusin, ME 2000. Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine foetuses. Biology of Reproduction 63, 1787–1794.Google Scholar
Hossein, MS, Jeong, YW, Park, SW, Kim, JJ, Lee, E, Ko, KH, Hyuk, P, Hoon, SS, Kim, YW, Hyun, SH, Shin, T and Hwang, WS 2009. Birth of beagle dogs by somatic cell nuclear transfer. Animal Reproduction Science 114, 404–414.Google Scholar
Hosseini, SM, Moulavi, F, Foruzanfar, M, Hajian, M, Abedi, P, Rezazade-Valojerdi, M, Parivar, K, Shahverdi, AH and Nasr-Esfahani, MH 2008. Effect of donor cell type and gender on the efficiency of in vitro sheep somatic cell cloning. Small Ruminant Research 78, 162–168.Google Scholar
Hufana-Duran, D, Pedro, PB, Venturina, HV, Hufana, RD, Salazar, RD, Duran, PG and Cruz, LC 2004. Post-warming hatching and birth of live calves following transfer of in vitro derived vitrified water buffalo (Bubalus bubalis) embryos. Theriogenology 61, 1429–1439.Google Scholar
Hufana-Duran, D, Pedro, PB, Salazar, AL Jr, Venturina, HV, Duran, PG, Takahashi, Y, Kanai, Y and Cruz, LC 2008. Twin calf production in water buffaloes following non-surgical transfer of in vitro-produced-vitrified embryos. Philippine Journal of Science 137 (2), 99–104.Google Scholar
Kamonpatana, M 2003. Artificial breeding: IVM/IVF/IVC/ET/cloning in buffalo as comparison to cattle in Thailand. Buffalo Journal (suppl. 3), 59–77.Google Scholar
Kamonpatana, M, Chantaraprateep, P and Ngramsuriyaroj, C 1981. A herd test for non-pregnancy using plasma progesterone levels in the selection of swamp buffalo for oestrus synchronization. British Veterinary Journal 137, 173–175.CrossRefGoogle ScholarPubMed
Kato, Y, Tani, T, Sotomaru, Y, Kurokawa, K, Kato, J-Y, Doguchi, H, Yasue, H and Tsunoda, Y 1998. Eight calves cloned from somatic cells of a single adult. Science 282, 2095–2098.Google Scholar
Kubota, C, Yamakuchi, H, Todoroki, J, Mizoshita, K, Tabara, N, Barber, M and Yang, X 2000. Six cloned calves produced from adult fibroblast cells after long-term culture. Proceedings of the National Academy of Sciences of the United States of America 97 (3), 990–995.Google Scholar
Lu, F, Shi, D, Wei, J, Yang, S and Wei, Y 2005. Development of embryos reconstructed by interspecies nuclear transfer of adult fibroblasts between buffalo (Bubalus bubalis) and cattle (Bos indicus). Theriogenology 64, 1309–1319.Google Scholar
Lu, F, Jiang, J, Li, N, Zhang, S, Sun, H, Luo, C, Wei, Y and Shi, D 2011. Effects of recipient oocyte age and interval from fusion to activation on development of buffalo (Bubalus bubalis) nuclear transfer embryos derived from fetal fibroblasts. Theriogenology 76, 967–974.CrossRefGoogle ScholarPubMed
Parnpai, R and Tasripoo, K 2003. Effects of different activation protocols on the development of cloned swamp buffalo embryos derived from granulosa cells. Theriogenology 59, 279.Google Scholar
Parnpai, R, Tasripoo, K and Kamonpatana, M 1999. Development of cloned swamp buffalo embryos derived from fetal fibroblasts: comparison in vitro cultured with or without buffalo and cattle oviductal epithelial cells. Buffalo Journal 15 (3), 371–384.Google Scholar
Parnpai, R, Tasripoo, K and Kamonpatana, M 2000. Comparison of cloning swamp buffalo embryos fetal fibroblasts and granulosa cells as donor cell. Proceedings of 14th International Congress on Animal Reproduction, Stockholm, Sweden, 2–6 July, 241pp.Google Scholar
Parnpai, R, Tasripoo, K and Kamonpatana, M 2001. Developmental potential of vitrified cloned swamp buffalo morulae derived from granulosa cells. Theriogenology 55, 284.Google Scholar
Parnpai, R, Tasripoo, K and Kamonpatana, M 2002. Comparison of cloning efficiency in bovine and swamp buffalo embryo using fetal fibroblast, ear fibroblasts and granulose cell. Theriogenology 57, 443.Google Scholar
Powell, AM, Talbot, NC, Well, KD, Kerr, DE, Pursel, VG and Wall, RJ 2004. Cell donor influences success of producing cattle by somatic cell nuclear transfer. Biology of Reproduction 71, 210–216.Google Scholar
Promdireg, A, Presicce, GA, De Rensis, F, Singlor, J and Techakumphu, M 2008. Follicular dynamics following estrus synchronization in swamp buffalo cows (Bubalus bubalis). The Thai Journal of Veterinary Medicine 38 (4), 25–34.CrossRefGoogle Scholar
Rodriguez-Osorio, N, Urrego, R, Cibelli, JB, Eilertsen, K and Memili, E 2012. Reprogramming mammalian somatic cells. Theriogenology 78 (9), 1869–1886.Google Scholar
Saikhun, J, Kitiyanant, N, Songtaveesin, C, Pavasuthipaisit, K and Kitiyanant, Y 2004. Development of swamp buffalo (Bubalus babalis) embryos after parthenogenetic activation and nuclear transfer using serum fed or starved fetal fibroblasts. Reproduction Nutrition and Development 44, 65–78.Google Scholar
Schubbert, R and Rittler, S 2012. Multiplex microsatellite analysis with 5’ dye-labelled marker sets using fam, Yakima yellow, atto 550, atto 565 and atto 633 on an ABI 3130XL genetic analyser., eurofins mwg/operon, ABI 3130XL user manual, pp. 1–5. Retrieved February 2012, from www.eurofinsdna.com.Google Scholar
Shi, D, Lu, F, Wei, Y, Cui, K, Yang, S, Wei, J and Liu, Q 2007. Buffaloes (Bubalus bubalis) cloned by nuclear transfer of somatic cells. Biology of Reproduction 77, 285–289.Google Scholar
Singla, SK, Singh, B, Shah, RA, George, A, Chauhan, MS, Manik, RS and Palta, P 2010. Cloning in buffalo-scope and limitation. Proceedings of the International Buffalo Congress, New Delhi, India, 1–4 February, pp. 70–75.Google Scholar
Tasripoo, K, Srisakwattana, K, Sophon, S, Nualchuen, W and Usawang, S 2007. Cloning of buffalo fibroblast cell from donor of different ages. Buffalo Journal 23 (2), 141–152.Google Scholar
Thevarnanoharan, K, Vandepitte, W, Mohiuddin, G and Chantalakhana, C 2001. Environmental factors affecting various growth traits of swamp buffalo calves. Pakistan Journal of Agricultural Science 38 (3–4), 5–10.Google Scholar
Vecchio, D, Di Palo, R, Zicaralli, L, Grassi, C, Cammarano, A, D’Occhio, MJ and Campanile, G 2007. Embryonic mortality in buffalo naturally mated. Italian Journal of Animal Science 6 (suppl. 2, Part 2), 677–679.Google Scholar
Virakul, P 1987. Conception, gestation and parturition in swamp buffalo. In Swamp buffalo reproduction, 2nd edition (ed. P Chantaraprateep, P Virakul, C Lohachit and A Kunavongkrit), pp. 211–224. Chulalongkorn University Printing House, Bangkok, Thailand.Google Scholar
Wilmut, I, Schnieke, AE, McWhir, J, Kind, AJ and Campbell, KH 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810–813.Google Scholar
Zimmermann, U and Vienken, J 1982. Electric field-induced cell-to-cell fusion. Journal of Membrane Biology 67, 165–182.Google Scholar
Supplementary material: File

Tasripoo Supplementary Material

Figure S1

Download Tasripoo Supplementary Material(File)
File 167.4 KB
Supplementary material: File

Tasripoo Supplementary Material

Figure S2

Download Tasripoo Supplementary Material(File)
File 181.2 KB
Supplementary material: File

Tasripoo Supplementary Material

Figure S3

Download Tasripoo Supplementary Material(File)
File 393.2 KB
Supplementary material: File

Tasripoo Supplementary Material

Figure S4

Download Tasripoo Supplementary Material(File)
File 264.7 KB
Supplementary material: File

Tasripoo Supplementary Material

Figure S5

Download Tasripoo Supplementary Material(File)
File 539.1 KB
Supplementary material: File

Tasripoo Supplementary Material

Figure S6

Download Tasripoo Supplementary Material(File)
File 2.6 MB
Supplementary material: File

Tasripoo Supplementary Material

Figure S7

Download Tasripoo Supplementary Material(File)
File 197.1 KB
Supplementary material: File

Tasripoo Supplementary Material

Supplementary Material

Download Tasripoo Supplementary Material(File)
File 31.2 KB