Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-24T00:47:51.522Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of the VP22 gene adjuvant for enhancement of DNA vaccine against somatostatin in mice

Published online by Cambridge University Press:  01 November 2008

Li. Han ,
A. X. Liang ,
J. Zhang ,
M. Fang ,
G. H. Hua ,
L. Sang ,
L. Y. Geng ,
H. R. Wang  and
L. G. Yang
Li. Han
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
A. X. Liang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
J. Zhang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
M. Fang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
G. H. Hua
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
L. Sang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
L. Y. Geng
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
H. R. Wang
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
L. G. Yang*
Affiliation:
Key lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 PRChina
*
E-mail: [email protected]
Get access

Abstract

Somatostatin (SS) is a hormone that inhibits the secretion of growth hormone. Immunization against SS can promote the growth of animals. A novel SS-VP22 fused vaccine, pEGS2SS-V, was constructed from pEGS2SS plasmid with a VP22 gene fragment. Two times of immunization with pEGS2SS-V-induced anti-SS antibodies in mice. Compared with mice immunized with pEGS2SS and 0.85% saline, the growth performance of mice immunized with pEGS2SS-V was increased by 14.1% (P < 0.05) and 48.4% (P < 0.01) on the 2nd week after the first vaccination, respectively. The results indicated that the effects of the somatostatin DNA vaccine could be improved effectively by VP22 gene adjuvant.

Keywords

BHV-1 VP22somatostatinDNA vaccine
Type
Full Paper
Information
animal , Volume 2 , Issue 11 , November 2008 , pp. 1569 - 1574
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ayuk, J, Sheppard, MC 2006. Growth hormone and its disorders. Postgraduate Medical Journal 82, 24–30.CrossRefGoogle ScholarPubMed
Buonomo, FC, Sabacky, MJ, Della-Fera, MA, Baile, CA 1987. Effects of somatostatin immunoneutralization on growth and endocrine parameters in chickens. Domestic Animal Endocrinology 4, 191–200.Google ScholarPubMed
Calarota, S, Bratt, G, Nordlund, S, Hinkula, J, Leandersson, AC, Sandstrom, E, Wahren, B 1998. Cellular cytotoxic response induced by DNA vaccination in HIV-1-infected patients. Lancet 351, 1320–1325.CrossRefGoogle ScholarPubMed
Davis, HL, Weeratna, R, Waldschmidt, TJ, Tygrett, L, Schorr, J, Krieg, AM 1998. CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen. Journal of Immunology 160, 870–876.CrossRefGoogle ScholarPubMed
Han L, Mao DG, Zhang DK, Liang AX, Fang M, Moaeen-Ud-Din M and Yang LG 2007. Development and evaluation of a novel DNA vaccine expressing inhibin alpha (1–32) fragment for improving the fertility in rats and sheep. Animal Reproduction Science (Epub ahead of print).Google Scholar
Harms, JS, Ren, X, Oliveira, SC, Splitter, GA 2000. Distinctions between bovine herpesvirus 1 and herpes simplex virus type 1 VP22 tegument protein subcellular associations. Journal of Virology 74, 3301–3312.Google ScholarPubMed
Hung, CF, Cheng, WF, Chai, CY, Hsu, KF, He, L, Ling, M, Wu, TC 2001. Improving vaccine potency through intercellular spreading and enhanced MHC class I presentation of antigen. Journal of Immunology 166, 5733–5740.CrossRefGoogle ScholarPubMed
Hung, CF, He, L, Juang, J, Lin, TJ, Ling, M, Wu, TC 2002. Improving DNA vaccine potency by linking Marek’s disease virus type 1 VP22 to an antigen. Journal of Virology 76, 2676–2682.CrossRefGoogle Scholar
Kim, TW, Hung, CF, Kim, JW, Juang, J, Chen, PJ, He, L, Boyd, DA, Wu, TC 2004. Vaccination with a DNA vaccine encoding herpes simplex virus type 1 VP22 linked to antigen generates long-term antigen-specific CD8-positive memory T cells and protective immunity. Human Gene Therapy 15, 167–177.CrossRefGoogle ScholarPubMed
Liang, X, Chow, B, Li, Y, Raggo, C, Yoo, D, Attah-Poku, S, Babiuk, LA 1995. Characterization of bovine herpesvirus 1 UL49 homolog gene and product: bovine herpesvirus 1 UL49 homolog is dispensable for virus growth. Journal of Virology 69, 3863–3867.CrossRefGoogle ScholarPubMed
Liang A, Cao S, Han L, Yao Y, Moaeen-Ud-Din M and Yang L 2008. Construction and evaluation of the eukaryotic expression plasmid encoding two copies of somatostatin genes fused with hepatitis B surface antigen gene S. Vaccine 26, 2935–2941.CrossRefGoogle Scholar
MacGregor, RR, Boyer, JD, Ugen, KE, Lacy, KE, Gluckman, SJ, Bagarazzi, ML, Chattergoon, MA, Baine, Y, Higgins, TJ, Ciccarelli, RB, Coney, LR, Ginsberg, RS, Weiner, DB 1998. First human trial of a DNA-based vaccine for treatment of human immunodeficiency virus type 1 infection: safety and host response. Journal of Infectious Diseases 178, 92–100.CrossRefGoogle ScholarPubMed
Michel, N, Osen, W, Gissmann, L, Schumacher, TN, Zentgraf, H, Muller, M 2002. Enhanced immunogenicity of HPV 16 E7 fusion proteins in DNA vaccination. Virology 294, 47–59.CrossRefGoogle ScholarPubMed
Ohlsson, C, Bengtsson, BA, Isaksson, OG, Andreassen, TT, Slootweg, MC 1998. Growth hormone and bone. Endocrine Reviews 19, 55–79.Google ScholarPubMed
Oliveira, SC, Harms, JS, Afonso, RR, Splitter, GA 2001. A genetic immunization adjuvant system based on BVP22-antigen fusion. Human Gene Therapy 12, 1353–1359.CrossRefGoogle ScholarPubMed
Saha, S, Yoshida, S, Ohba, K, Matsui, K, Matsuda, T, Takeshita, F, Umeda, K, Tamura, Y, Okuda, K, Klinman, D, Xin, KQ, Okuda, K 2006. A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus. Virology 354, 48–57.CrossRefGoogle ScholarPubMed
Tang, DC, DeVit, M, Johnston, SA 1992. Genetic immunization is a simple method for eliciting an immune response. Nature 356, 152–154.CrossRefGoogle ScholarPubMed
Van Kessel, AG, Korchinski, RS, Hampton, CH, Laarveld, B 1990. Effect of immunization against somatostatin in the pregnant ewe on growth and endocrine status of the neonatal lamb. Domestic Animal Endocrinology 7, 217–227.CrossRefGoogle ScholarPubMed
Vance, ML, Hartman, ML, Thorner, MO 1992. Growth hormone and nutrition. Hormone Research 38 (suppl 1), 85–88.CrossRefGoogle ScholarPubMed
Wang, R, Doolan, DL, Le, TP, Hedstrom, RC, Coonan, KM, Charoenvit, Y, Jones, TR, Hobart, P, Margalith, M, Ng, J, Weiss, WR, Sedegah, M, de Taisne, C, Norman, JA, Hoffman, SL 1998. Induction of antigen-specific cytotoxic T lymphocytes in humans by a malaria DNA vaccine. Science 282, 476–480.CrossRefGoogle ScholarPubMed
Wills, KN, Atencio, IA, Avanzini, JB, Neuteboom, S, Phelan, A, Philopena, J, Sutjipto, S, Vaillancourt, MT, Wen, SF, Ralston, RO, Johnson, DE 2001. Intratumoral spread and increased efficacy of a p53-VP22 fusion protein expressed by a recombinant adenovirus. Journal of Virology 75, 8733–8741.CrossRefGoogle ScholarPubMed
Zheng, C, Babiuk, LA, van Drunen Littel-van den Hurk, S 2005. Bovine herpesvirus 1 VP22 enhances the efficacy of a DNA vaccine in cattle. Journal of Virology 79, 1948–1953.CrossRefGoogle ScholarPubMed