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Evaluation of methodological aspects of digestibility measurements in ponies fed different haylage to concentrate ratios

Published online by Cambridge University Press:  17 April 2017

F. J. W. C. Schaafstra ,
D. A. van Doorn ,
J. T. Schonewille ,
M. M. J. van Riet ,
P. Visser ,
M. C. Blok  and
W. H. Hendriks
F. J. W. C. Schaafstra*
Affiliation:
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CM Utrecht, The Netherlands
D. A. van Doorn
Affiliation:
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CM Utrecht, The Netherlands Department of Equine Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584 CM Utrecht, The Netherlands
J. T. Schonewille
Affiliation:
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CM Utrecht, The Netherlands
M. M. J. van Riet
Affiliation:
Animal Nutrition Group, Wageningen University, Elst 1, 6708 WD Wageningen, The Netherlands
P. Visser
Affiliation:
Department of Equine Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584 CM Utrecht, The Netherlands
M. C. Blok
Affiliation:
Productschap Diervoeder, Elst 1, 6708 WD, Wageningen, The Netherlands
W. H. Hendriks
Affiliation:
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CM Utrecht, The Netherlands Animal Nutrition Group, Wageningen University, Elst 1, 6708 WD Wageningen, The Netherlands
*
†E-mail: [email protected]
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Abstract

Methodological aspects of digestibility measurements were studied in four Welsh pony geldings consuming haylage-based diets with increasing proportions of a pelleted concentrate according to a 4×4 Latin square design experiment. Ponies were fed four experimental, iso-energetic (net energy (NE) basis) diets (i.e. 22 MJ NE/day) with increasing proportions of a pelleted concentrate (C) in relation to haylage (H). The absolute amounts of diet dry matter fed per day were 4.48 kg of H (100H), 3.36 and 0.73 kg of H and C (75H25C), 2.24 and 1.45 kg of H and C (50H50C) and 1.12 and 2.17 kg of H and C (25H75C). Diets were supplemented with minerals, vitamins and TiO2 (3.7 g Ti/day). Voluntary voided faeces were quantitatively collected daily during 10 consecutive days and analysed for moisture, ash, ADL, acid-insoluble ash (AIA) and Ti. A minimum faeces collection period of 6 consecutive days, along with a 14-day period to adapt the animals to the diets and become accustomed to the collection procedure, is recommended to obtain accurate estimations on dry matter digestibility and organic matter digestibility (OMD) in equids fed haylage-based diets supplemented with concentrate. In addition, the recovery of AIA, ADL and Ti was determined and evaluated. Mean faecal recovery over 10 consecutive days across diets for AIA, ADL and Ti was 124.9% (SEM 2.9), 108.7% (SEM 2.0) and 97.5% (SEM 0.9), respectively. Cumulative faecal recovery of AIA significantly differed between treatments, indicating that AIA is inadequate to estimate the OMD in equines. In addition, evaluation of the CV of mean cumulative faecal recoveries obtained by AIA, ADL and Ti showed greater variations in faecal excretion of AIA (9.1) and ADL (7.4) than Ti (3.7). The accuracy of prediction of OMD was higher with the use of Ti than ADL. The use of Ti is preferred as a marker in digestibility trials in equines fed haylage-based diets supplemented with increasing amounts of pelleted concentrate.

Keywords

dietary markersdigestibilityequinesrecoverytotal faeces collection
Type
Research Article
Information
animal , Volume 11 , Issue 11 , November 2017 , pp. 1922 - 1929
Copyright
© The Animal Consortium 2017 

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References

Agazzi, A, Ferroni, M, Fanelli, A, Maroccolo, S, Invernizzi, G, Dell’Orto, V and Savoini, G 2011. Evaluation of the effects of live yeast supplementation on apparent digestibility of high-fiber diet in mature horses using the acid insoluble ash marker modified method. Journal of Equine Veterinary Science 31, 13–18.Google Scholar
Araújo, KV, de Freitas Lima, JA, Fiahlo, ET and Miyagi, ES 2000. Comparação dos indicadores internos com o método de coleta total para determinar a digestibilidade dos nutrientes de dietas mistas em eqüinos. Ciênca e Agrotecnologia 24, 1041–1048 (in Portuguese).Google Scholar
Centraal Veevoederbureau (CVB) 2004. Het EWpa en VREp systeem. CVB documentatierapport No. 31, Centraal Veevoederbureau, Lelystad, the Netherlands (in Dutch).Google Scholar
Dove, H and Mayes, RW 1991. The use of plant waxes as marker substances in studies of the nutrition of herbivores: A review. Australian Journal of Agricultural Research 42, 913–952.Google Scholar
Drougal, C, de Fombelle, A and Julliand, V 2001. Feeding and microbial disorders in horses: 2: Effect of three hay:grain ratios on digesta passage rate and digestibility in horses. Journal of Equine Veterinary Science 21, 487–491.Google Scholar
Fahey, GC and Jung, HG 1983. Lignin as a marker in digestion studies: A review. Journal of Animal Science 57, 220–225.Google Scholar
Fukushima, RS and Dehority, BA 2000. Feasibility of using lignin isolated from forages by solubilization in acetyl bromide as a standard for lignin analysis. Journal of Animal Science 78, 3135–3143.Google Scholar
Goachet, AG, Philippeau, C, Varloud, M and Julliand, V 2009. Adaptations to standard approaches for measuring total tract apparent digestibility and gastro-intestinal retention time in horses in training. Animal Feed Science and Technology 152, 141–151.Google Scholar
International Organization for Standardization (ISO) 1978. Animal feeding stuffs. Determination of crude ash. ISO 5984, International Organization for Standardization, Geneva, Switzerland.Google Scholar
International Organization for Standardization (ISO) 1981. Animal feeding stuffs. Determination of crude fiber content–General method. ISO 5498, International Organization for Standardization, Geneva, Switzerland.Google Scholar
International Organization for Standardization (ISO) 1983. Animal feeding stuffs. Determination of moisture content. ISO 6496, International Organization for Standardization, Geneva, Switzerland.Google Scholar
International Organization for Standardization (ISO) 1999. Animal feeding stuffs. Determination of crude fat. ISO 6492, International Organization for Standardization, Geneva, Switzerland.Google Scholar
International Organization for Standardization (ISO) 2005a. Animal feeding stuffs. Determination of nitrogen and calculation of crude protein content–Part 1: Kjeldahl method. ISO 5983-1, International Organization for Standardization, Geneva, Switzerland.Google Scholar
International Organization for Standardization (ISO) 2005b. Animal feeding stuffs. Determination of nitrogen and calculation of crude protein content–Part 2: Block digestion/steam distillation method. ISO 5983-2, International Organization for Standardization, Geneva, Switzerland.Google Scholar
Martin-Rosset, W, Andrieu, J, Vermorel, M and Dulphy, JP 1984. Valeur nutritive des aliments pour le cheval. Le Cheval–Reproduction, Sélection, Alimentation, Exploitation. INRA Publications, Versailles, France.Google Scholar
Martin-Rosset, W and Dulphy, JP 1987. Digestibility interactions between forages and concentrates in horses: influence of feeding level–comparison with sheep. Livestock Production Science 17, 263–276.Google Scholar
Miraglia, N, Bergero, D, Bassano, B, Tarantola, M and Ladetto, G 1999. Studies of apparent digestibility in horses and the use of internal markers. Livestock Production Science 60, 21–25.Google Scholar
Muntifering, RB 1982. Evaluation of various lignin assays for determining ruminal digestion of roughages in lambs. Journal of Animal Science 55, 432–438.Google Scholar
Myers, WD, Ludden, PA, Nayigihugu, V and Hess, BW 2004. Technical note: a procedure for the preparation and quantitative analysis of samples for titanium dioxide. Journal of Animal Science 82, 179–183.Google Scholar
Myers, WD, Ludden, PA, Nayigihugu, V and Hess, BW 2006. Excretion patterns of titanium dioxide and chromic oxide in duodenal digesta and feces of ewes. Small Ruminant Research 63, 135–141.Google Scholar
National Research Council (NRC) 2007. Nutrient Requirements of Horses, 6th revised edition. The National Academies Press, Washington, DC, USA.Google Scholar
Nederlandse Norm (NEN) 1974. Test methods for feeding stuffs. Determination of reducing sugar, crude total sugar, saccharose and lactose. NEN 3571. Standards of the Netherlands Normalization Institute, Delft, the Netherlands.Google Scholar
Oliveira, C, de Almeida, FQ, de Filho, S, Vieira, AA, de Almeida, MI, Corassa, A, Lopes, BA and Macedo, R 2003. Estimativa da digestibilidade aparante de nutrients em dieta para eqïinos com o uso de óxida crômico e indicadores internos. Revista Brasileira de Zootecnia 32, 1681–1689 (in Portuguese).Google Scholar
Palmgren Karlsson, C, Lindberg, JE and Rundgren, M 2000. Associative effects on total digestibility in horses fed different rations of grass hay and whole oats. Livestock Production Science 65, 143–153.Google Scholar
Pina, DS, Valadares Filho, SC, Tedeschi, LO, Barbosa, AM and Valadares, RFD 2009. Influence of different levels of concentrate and ruminally undegraded protein on digestible variables in beef heifers. Journal of Animal Science 87, 1058–1067.Google Scholar
Robbins, KR, Saxton, AM and Southern, LL 2006. Estimation of nutrient requirements using broken-line regression analysis. Journal of Animal Science 84 (E. suppl.), E155–E165.Google Scholar
Sales, J 2012. A review on the use of indigestible dietary markers to determine total tract apparent digestibility of nutrients in horses. Animal Feed Science and Technology 174, 119–130.Google Scholar
Sales, J and Janssens, GPJ 2003. The use of markers to determine energy metabolizability and nutrient digestibility in avian species. World’s Poultry Science Journal 59, 314–327.Google Scholar
Sampaio, CB, Detmann, E, Valente, TNP, de Souza, MA, Valaderes Filho, SC and Paulino, MF 2011. Recuperação fecal e vício de tempo longo de indicadores internos e externos em ensaio de digestão com bovinos. Revista Brasileira de Zootecnia 40, 174–182 (in Portuguese).Google Scholar
Schaafstra, FJWC, van Doorn, DA, Schonewille, JT, Wartena, FC, Zoon, MV, Blok, MC and Hendriks, WH 2015. Evaluation of methodological aspects of digestibility measurements in ponies fed different grass hays. Journal of Animal Science 93, 4742–4749.CrossRefGoogle ScholarPubMed
Schneider, BH and Flatt, WP 1975. The evaluation of feeds through digestibility experiments. The University of Georgia Press, Athens, GA, USA.Google Scholar
Siqueira, RF, Gomes, RC, Rodrigues, PHM, Fukushima, RS, Lorenzo, CLF and Gobesso, AAO 2009. Uso da cutina na estimative da digestibilidade apparent de dietas para equinos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61, 1373–1381 (in Portuguese).Google Scholar
Smeets, N, Nuyens, F, Van Campenhout, L, Delezie, E, Pannecoucque, J and Niewold, T 2015. Relationship between wheat characteristics and nutrient digestibility in broilers: comparison between total collection and marker (titanium dioxide) technique. Poultry Science 94, 1584–1591.Google Scholar
Sweeting, MP and Houpt, KA 1987. Water consumption and time budgets of stabled pony (Equus caballus) geldings. Applied Animal Behaviour Science 17, 1–7.CrossRefGoogle Scholar
Tasker, JB 1965. Fluid and electrolyte studies in the horse. II. An apparatus for the collection of total daily urine and feces from horses. The Cornell Veterinarian 57, 77–84.Google Scholar
Titgemeyer, EC, Armendariz, CK, Bindel, DJ, Greenwood, RH and Löest, CA 2001. Evaluation of titanium dioxide as a digestibility marker for cattle. Journal of Animal Science 79, 1059–1063.Google Scholar
Tyler, SJ 1972. The behaviour and social organization of the New Forest ponies. Animal Behaviour Monographs 5, 85–196.Google Scholar
Van Keulen, J and Young, BA 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science 44, 282–287.Google Scholar
Van Soest, PJ 1994. Nutritional ecology of the ruminant, 2nd edition. Cornell University Press, Ithaca, NY, USA.CrossRefGoogle Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.Google Scholar