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Effects of olive and fish oil Ca soaps in ewe diets on milk fat and muscle and subcutaneous tissue fatty-acid profiles of suckling lambs

Published online by Cambridge University Press:  27 February 2014

B. Gallardo ,
P. Gómez-Cortés ,
A. R. Mantecón ,
M. Juárez ,
T. Manso  and
M. A. de la Fuente
B. Gallardo
Affiliation:
ETS Ingenierías Agrarias, Universidad de Valladolid, 34004 Palencia, Spain
P. Gómez-Cortés
Affiliation:
Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM), 28049 Madrid, Spain
A. R. Mantecón
Affiliation:
Instituto de Ganadería de Montaña (CSIC-ULE), 24346 Grulleros, León, Spain
M. Juárez
Affiliation:
Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM), 28049 Madrid, Spain
T. Manso
Affiliation:
ETS Ingenierías Agrarias, Universidad de Valladolid, 34004 Palencia, Spain
M. A. de la Fuente*
Affiliation:
Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM), 28049 Madrid, Spain
*
† E-mail: [email protected]
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Abstract

Enhancing healthy fatty acids (FAs) in ewe milk fat and suckling lamb tissues is an important objective in terms of improving the nutritional value of these foods for the consumer. The present study examined the effects of feeding-protected lipid supplements rich in unsaturated FAs on the lipid composition of ewe milk, and subsequently in the muscle and subcutaneous adipose tissues of lambs suckling such milk. Thirty-six pregnant Churra ewes with their new-born lambs were assigned to one of three experimental diets (forage/concentrate ratio 50 : 50), each supplemented with either 3% Ca soap FAs of palm (Control), olive (OLI) or fish (FO) oil. The lambs were nourished exclusively by suckling for the whole experimental period. When the lambs reached 11 kg BW, they were slaughtered and samples were taken from the Longissimus dorsi and subcutaneous fat depots. Although milk production was not affected by lipid supplementation, the FO diet decreased fat content (P<0.001), whereas the OLI milk FA profile resembled that of the Control diet. In contrast, although FO drastically diminished the contents of stearic and oleic acids (P<0.001), all the saturated even-numbered carbon FAs from 6:0 to 14:0 increased (P<0.05). FO also produced the highest levels of c9,t11-18:2 (2.21%) and n-3 FAs, 20:5 n-3 (0.58%), 22:5 n-3 (0.48%) and 22:6 n-3 (0.40%). The high levels of trans-11 18:1 (7.10%) obtained from the FO diet would suggest that Ca soaps only confer partial protection in the rumen. In contrast, the lack of significant differences in trans-10 18:1 levels (P>0.05) and other trans-FAs between Control and FO treatments would indicate that FO treatment does not alter rumen biohydrogenation pathways under the assayed conditions. Changes in dam milk FA composition induced differences in the FA profiles of meat and fat depots of lambs, preferentially incorporated polyunsaturated FAs into the muscle rather than storing them in the adipose tissue. In the intramuscular fat of the FO treatment, all the n-3 FAs reached their highest concentrations: 0.97 (18:3 n-3), 2.72 (20:5 n-3), 2.21 (22:5 n-3) and 1.53% (22:6 n-3). In addition, not only did FO intramuscular fat have the most cis-9, trans-11 18:2 (1.66%) and trans-11 18:1 (3.75%), but also the lowest n-6/n-3 ratio (1.80) and saturated FA content were not affected. Therefore, FO exhibited the best FA profile from a nutritional point of view.

Keywords

suckling lambfatty acidmilkintramuscular fatCa soap
Type
Full Paper
Information
animal , Volume 8 , Issue 7 , July 2014 , pp. 1178 - 1190
Copyright
© The Animal Consortium 2014 

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References

AbuGhazaleh, AA and Jenkins, TC 2004. Docosahexaenoic acid promotes vaccenic acid accumulation in mixed rumen cultures when incubated with linoleic acid. Journal of Dairy Science 87, 1047–1050.CrossRefGoogle ScholarPubMed
Ashes, JR, Sieber, BD, Gulati, SK, Cuthberson, AZ and Scott, TW 1992. Incorporation of n-3 fatty acids of fish oil into tissue and serum lipids of ruminants. Lipids 27, 629–631.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists 2003. Official method of analysis of the Association of Official Agricultural Chemists, 17th edition. AOAC International, Gaithersburg, MD, USA.Google Scholar
Awawdeh, MS, Obeidat, BS and Kridli, RT 2009. Yellow grease as an alternative energy source for nursing Awassi ewes and their suckling lambs. Animal Feed Science and Technology 152, 165–174.CrossRefGoogle Scholar
Bichi, E, Toral, PG, Hervás, G, Frutos, P, Gómez-Cortés, P, Juárez, M and De la Fuente, MA 2012. Inhibition of ∆9-desaturase activity with sterculic acid: effect on the endogenous synthesis of cis-9 18:1 and cis-9 trans-11 18:2 in dairy sheep. Journal of Dairy Science 95, 5242–5252.CrossRefGoogle Scholar
Bligh, EG and Dyer, WJ 1959. A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.CrossRefGoogle ScholarPubMed
Casals, R, Caja, G, Pol, MV, Such, X, Albanell, E, Gargouri, G and Casellas, J 2006. Response of lactating dairy ewes to various levels of dietary calcium soaps of fatty acids. Animal Feed Science and Technology 131, 312–332.CrossRefGoogle Scholar
Doreau, M, Fievez, V, Troegeler-Meynadier, A and Glasser, F 2012. Métabolisme ruminal et digestion des acides gras longs chez le ruminant: le point des connaissances récentes. INRA Productions Animales 25, 361–374.CrossRefGoogle Scholar
Gama, MAS, Garnsworthy, PC, Griinari, JM, Leme, PR, Rodrigues, PHM, Souza, LWO and Lanna, DPD 2008. Diet-induced milk fat depression: association with changes in milk fatty acid composition and fluidity of milk fat. Livestock Science 115, 319–331.CrossRefGoogle Scholar
Gargouri, A, Caja, G, Casals, R and Mezghan, I 2006. Lactational evaluation of effects of calcium soap of fatty acids on dairy ewes. Small Ruminant Research 66, 1–10.CrossRefGoogle Scholar
Gómez-Cortés, P, Frutos, P, Mantecón, AR, Juárez, M, De la Fuente, MA and Hervás, G 2008. Addition of olive oil to dairy ewe diets: effect on milk fatty acid profile and animal performance. Journal of Dairy Science 91, 3119–3127.CrossRefGoogle ScholarPubMed
Gulati, SK, Ashes, JR and Scott, TW 1999. Hydrogenation of eicosapentaenoic and docosahexaenoic acids and their incorporation into milk fat. Animal Feed Science and Technology 79, 57–64.CrossRefGoogle Scholar
Hussein, M, Harvatine, KH, Weerasinghe, PB, Sinclair, LA and Bauman, DE 2013. Conjugated linoleic acid-induced milk fat depression lactating ewes is accompanied by reduced expression of mammary genes involved in lipid synthesis. Journal of Dairy Science 96, 3825–3834.CrossRefGoogle ScholarPubMed
International Dairy Federation 2000. International IDF Standard 141C:2000. Determination of milk fat, protein and lactose content. Guidance on the operation of mid-infrared instruments. International Dairy Federation, Brussels, Belgium.Google Scholar
Jenkins, TC and Bridges, WC Jr. 2007. Protection of fatty acids against ruminal biohydrogenation in cattle. European Journal of Lipid Science and Technology 109, 778–789.CrossRefGoogle Scholar
Kitessa, SM, Peake, D, Bencini, R and Williams, AJ 2003. Fish oil metabolism in ruminants: III. Transfer of n-3 polyunsaturated fatty acids (PUFA) from tuna oil into sheep’s milk. Animal Feed Science and Technology 108, 1–14.CrossRefGoogle Scholar
Kitessa, SM, Gulati, SK, Ashes, JR, Fleck, E, Scott, TW and Nichols, PD 2001. Utilisation of fish oil in ruminants – I. Fish oil metabolism in sheep. Animal Feed Science and Technology 89, 189–199.CrossRefGoogle Scholar
Luna, P, Bach, A, Juárez, M and De la Fuente, MA 2008. Effect of a diet enriched in whole linseed and sunflower oil on goat milk fatty acid composition and conjugated linoleic acid isomer profile. Journal of Dairy Science 91, 20–28.CrossRefGoogle ScholarPubMed
Manso, T, Bodas, R, Vieira, C, Mantecón, AR and Castro, T 2011. Feeding vegetable oils to lactating ewes modifies the fatty acid profile of suckling lambs. Animal 5, 1659–1667.CrossRefGoogle ScholarPubMed
Mosley, EE, Powell, GL, Riley, MB and Jenkins, TC 2002. Microbial biohydrogenation of oleic acid to trans isomers in vitro. Journal of Lipid Research 43, 290–296.CrossRefGoogle ScholarPubMed
Or-Rashid, MM, Kramer, JKG, Wood, MA and McBride, BW 2008. Supplemental algal meal alters the ruminal trans-18:1 fatty acid and conjugated linoleic acid composition in cattle. Journal of Animal Science 86, 187–196.CrossRefGoogle Scholar
Osorio, MT, Zumalacárregui, JM, Figueira, A and Mateo, J 2007. Fatty acid composition in subcutaneous, intermuscular and intramuscular fat deposits of suckling lamb meat: effect of milk source. Small Ruminant Research 73, 127–134.CrossRefGoogle Scholar
Palmquist, DL, St-Pierre, N and McClure, KE 2004. Tissue fatty acid profiles can be used to quantify endogenous rumenic acid synthesis in lambs. Journal of Nutrition 134, 2407–2414.CrossRefGoogle ScholarPubMed
Pérez Alba, LM, De Souza Cavalcanti, S, Pérez Hernández, M, Martínez Marín, A and Fernández Marín, G 1996. Calcium soap of olive fatty acids in the diets of Manchega dairy ewes: effects on digestibility and production. Journal of Dairy Science 80, 3316–3324.CrossRefGoogle Scholar
Perfield, JW, Lock, AL, Griinari, JM, Sæbø, A, Delmonte, P, Dwyer, DA and Bauman, DE 2007. Trans-9, cis-11 conjugated linoleic acid reduces milk fat synthesis in lactating dairy cows. Journal of Dairy Science 90, 2211–2218.CrossRefGoogle ScholarPubMed
Pulina, G, Nudda, A, Battacone, G and Cannas, A 2006. Effects of nutrition on the contents of fat, protein, somatic cells, aromatic compounds, and undesirable substances in sheep milk. Animal Feed Science and Technology 131, 255–291.CrossRefGoogle Scholar
Scerra, M, Caparra, P, Foti, F, Galofaro, V, Sinatra, MC and Scerra, V 2007. Influence of ewe feeding systems on fatty acid composition of suckling lambs. Meat Science 76, 390–394.CrossRefGoogle ScholarPubMed
Shingfield, KJ, Bonnet, M and Scollan, ND 2013. Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7, 132–162.CrossRefGoogle ScholarPubMed
Shingfield, KJ, Sæbø, A, Sæbø, PC, Toivonen, V and Griinari, JM 2009. Effect of abomasal infusions of a mixture of octadecenoic acids on milk fat synthesis in lactating cows. Journal of Dairy Science 92, 4317–4329.CrossRefGoogle ScholarPubMed
Shingfield, KJ, Reynolds, CK, Hervás, G, Griinari, JM, Grandison, AS and Beever, DE 2006. Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. Journal of Dairy Science 89, 714–732.CrossRefGoogle ScholarPubMed
Simopoulos, AP 2008. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Experimental Biology and Medicine 233, 674–688.CrossRefGoogle ScholarPubMed
Sinclair, LA, Lock, AL, Early, R and Bauman, DE 2007. Effects of trans-10, cis-12 conjugated linoleic acid on ovine milk fat synthesis and cheese properties. Journal of Dairy Science 90, 3326–3335.CrossRefGoogle ScholarPubMed
Toral, PG, Frutos, P, Hervás, G, Gómez-Cortés, P, Juárez, M and De la Fuente, MA 2010a. Changes in milk fatty acid profile and animal performance in response to fish oil supplementation, alone or in combination with sunflower oil, in dairy ewes. Journal of Dairy Science 93, 1604–1615.CrossRefGoogle ScholarPubMed
Toral, PG, Hervás, G, Gómez-Cortés, P, Frutos, P, Juárez, M and De la Fuente, MA 2010b. Milk fatty acid profile and dairy sheep performance in response to diet supplementation with sunflower oil plus incremental levels of marine algae. Journal of Dairy Science 93, 1655–1667.CrossRefGoogle ScholarPubMed
Wood, JD, Enser, M, Fisher, AV, Nute, GR, Sheard, PR, Richardson, RI, Hughes, SI and Wittington, FM 2008. Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.CrossRefGoogle ScholarPubMed