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Effects of l-carnitine supplementation to suckling piglets on carcass and meat quality at market age

Published online by Cambridge University Press:  11 March 2013

D. Lösel  and
C. Rehfeldt
D. Lösel*
Affiliation:
Research Unit Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany
C. Rehfeldt
Affiliation:
Research Unit Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany
*
†E-mail: [email protected]
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Abstract

In a previous study, carnitine supplementation to piglets during the suckling period resulted in an increased total muscle fibre number at weaning in piglets of low birth weight. The objective of the present study was to investigate whether this effect is maintained until market age and whether this would attenuate the negative consequences of low birth weight on carcass and meat quality. Using a split-plot design with litter as block, sex as whole plot and treatment as subplot, the effects of early-postnatal l-carnitine supplementation on female and castrated male piglets of low birth weight were investigated on a total of 56 German Landrace piglets from 14 litters. From days 7 to 27 of age piglets were orally supplemented once daily with 400 mg of l-carnitine dissolved in 1 ml of water or received an equal volume of water without carnitine. From weaning (day 28) until slaughter (day 166 of age) all pigs were fed standard diets. At weaning, carnitine-supplemented piglets had a twofold increased concentration of free carnitine (P < 0.001) and a lower concentration of non-esterified fatty acids (P < 0.05) in blood plasma indicating that carnitine became bioavailable and increased fatty acid utilization during the period of supplementation. Growth performance was not influenced by treatment in any growth period. Dual-energy X-ray absorptiometry revealed no differences in body composition between groups in weeks 12, 16 and 20 of age. LW at slaughter, carcass weight, measures of meat yield and fat accretion, as well as body composition by chemical analyses and dissection of primal cuts did not differ between treatments. No differences between control and carnitine-treated pigs in total fibre number (P = 0.85) and fibre cross-sectional area (P = 0.68) in m. semitendinosus (ST) measured at slaughter could be observed. The carnitine group tended to exhibit a smaller proportion of slow-twitch oxidative fibres (P = 0.08), a greater proportion of fast-twitch glycolytic fibres (P = 0.11), and increased specific lactate dehydrogenase activity (P = 0.09) in ST indicating a more glycolytic muscle metabolism. Compared with the controls, a lower pH24 value was observed (P = 0.05) in ST muscle of carnitine-supplemented pigs, which – in castrates only – was associated with an increased drip loss (P < 0.01). Meat quality traits in m. longissimus were not influenced by treatment. In conclusion, our hypothesis that early-postnatal carnitine supplementation to piglets of low birth weight permanently increases myofibre number and improves later carcass and meat quality could not be confirmed by this experiment.

Keywords

piggrowthmuscle metabolismfibre numberfibre types
Type
Product quality, human health and well-being
Information
animal , Volume 7 , Issue 7 , July 2013 , pp. 1191 - 1198
Copyright
Copyright © The Animal Consortium 2013 

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References

Association of Official Analytical Chemists 2000. Official methods of analysis, vol. 2, 17th edition. AOAC, Arlington, VA, USA.Google Scholar
Bee, G 2004. Effect of early gestation feeding, birth weight, and gender of progeny on muscle fiber characteristics of pigs at slaughter. Journal of Animal Science 82, 826–836.CrossRefGoogle ScholarPubMed
Bérard, J, Kalbe, C, Lösel, D, Tuchscherer, A, Rehfeldt, C 2011. Potential sources of early-postnatal increase in myofibre number in pig skeletal muscle. Histochemistry and Cell Biology 136, 217–225.Google Scholar
Birkenfeld, C, Kluge, H, Eder, K 2006. l-carnitine supplementation of sows during pregnancy improves the suckling behaviour of their offspring. British Journal of Nutrition 96, 334–342.Google Scholar
Fischer, M, Varady, J, Hirche, F, Kluge, H, Eder, K 2009. Supplementation of l-carnitine in pigs: absorption of carnitine and effect on plasma and tissue carnitine concentrations. Archives of Animal Nutrition 63, 1–15.Google Scholar
Gondret, F, Lefaucheur, L, Juin, H, Louveau, I, Lebret, B 2006. Low birth weight is associated with enlarged muscle fiber area and impaired meat tenderness of the longissimus muscle in pigs. Journal of Animal Science 84, 93–103.Google Scholar
Heo, K, Lin, X, Odle, J, Han, IK 2000. Kinetics of carnitine palmitoyltransferase-I are altered by dietary variables and suggest a metabolic need for supplemental carnitine in young pigs. Journal of Nutrition 130, 2467–2470.Google Scholar
Hoffman, LA, Ivers, DJ, Ellersieck, MR, Veum, TL 1993. The effect of l-carnitine and soybean oil on performance and nitrogen and energy utilization by neonatal and young pigs. Journal of Animal Science 71, 132–138.CrossRefGoogle ScholarPubMed
Kim, JM, Lee, YJ, Choi, YM, Kim, BC, Yoo, BH, Hong, KC 2008. Possible muscle fiber characteristics in the selection for improvement in porcine lean meat production and quality. Asian-Australasian Journal of Animal Science 21, 1529–1534.Google Scholar
Kuhn, G, Ender, K, Nürnberg, K 1994. Influence of recombinant porcine somatotropin (rpST) on the chemical composition of the edible whole body and individual body fractions in pigs. Archiv für Tierzucht – Archives of Animal Breeding 37, 623–631.Google Scholar
Larzul, C, Lefaucheur, L, Ecolan, P, Gogué, J, Talmant, A, Sellier, P, Le Roy, P, Monin, G 1997. Phenotypic and genetic parameters for longissimus muscle fiber characteristics in relation to growth, carcass, and meat quality traits in Large White pigs. Journal of Animal Science 75, 3126–3137.Google Scholar
Lebret, B, Le Roy, P, Monin, G, Lefaucheur, L, Caritez, JC, Talmant, A, Elsen, JM, Sellier, P 1999. Influence of the three RN genotypes on chemical composition, enzyme activities, and myofiber characteristics of porcine skeletal muscle. Journal of Animal Science 77, 1482–1489.Google Scholar
Lefaucheur, L, Vigneron, P 1986. Post-natal changes in some histochemical and enzymatic characteristics of three pig muscles. Meat Science 16, 199–216.Google Scholar
Lefaucheur, L, Gerrard, D 2000. Muscle fibre plasticity in farm mammals. Journal of Animal Science 77, 1–19.Google Scholar
Lefaucheur, L, Edom, F, Ecolan, P, Butler-Browne, GS 1995. Pattern of muscle fiber type formation in the pig. Developmental Dynamics 203, 27–41.Google Scholar
Lösel, D, Kalbe, C, Rehfeldt, C 2009. l-carnitine supplementation during suckling intensifies the early postnatal skeletal myofiber formation in piglets of low birth weight. Journal of Animal Science 87, 2216–2226.CrossRefGoogle ScholarPubMed
Lösel, D, Kremer, P, Albrecht, E, Scholz, AM 2010. Comparison of a GE Lunar DPX-IQ and a Norland XR-26 dual energy X-ray absorptiometry scanner for body composition measurements in pigs – in vivo. Archiv für Tierzucht – Archives of Animal Breeding 53, 162–175.Google Scholar
Mascarello, F, Stecchini, ML, Rowlerson, A, Ballocchi, E 1992. Tertiary myotubes in postnatal growing pig muscle detected by their myosin isoform composition. Journal of Animal Science 70, 1806–1813.Google Scholar
Musser, RE, Goodband, RD, Tokach, MD, Owen, KQ, Nelssen, JL, Blum, SA, Campbell, RG, Smits, R, Dritz, SS, Civis, CA 1999. Effects of l-carnitine fed during lactation on sow and litter performance. Journal of Animal Science 77, 3296–3303.Google Scholar
Owen, KQ, Nelssen, JL, Goodband, RD, Weeden, TL, Blum, SA 1996. Effect of l-carnitine and soybean oil on growth performance and body composition of early-weaned pigs. Journal of Animal Science 74, 1612–1619.Google Scholar
Owen, KQ, Jit, H, Maxwell, CV, Nelssen, JL, Goodband, RD, Tokach, MD, Tremblay, GC, Koo, SI 2001. Dietary l-carnitine suppresses mitochondrial branched-chain keto acid dehydrogenase activity and enhances protein accretion and carcass characteristics of swine. Journal of Animal Science 79, 3104–3112.Google Scholar
Prieto, JA, Andrade, F, Aldamiz-Echevarria, L, Sanjurjo, P 2006. Determination of free and total carnitine in plasma by an enzymatic reaction and spectrophotometric quantitation spectrophotometric determination of carnitine. Clinical Biochemistry 39, 1022–1027.Google Scholar
Quiroz-Rothe, E, Rivero, JL 2004. Coordinated expression of myosin heavy chains, metabolic enzymes, and morphological features of porcine skeletal muscle fiber types. Microscopy Research and Technique 65, 43–61.Google Scholar
Rehfeldt, C, Kuhn, G 2006. Consequences of birth weight for postnatal growth performance and carcass quality in pigs as related to myogenesis. Journal of Animal Science 84, E113–E123.Google Scholar
Rehfeldt, C, Fiedler, I, Wegner, J 1987. Veränderungen der Mikrostruktur des Muskelgewebes bei Labormäusen, Rindern und Schweinen während des Wachstums. Zeitschrift für mikroskopisch-anatomische Forschung 101, 669–680.Google Scholar
Rehfeldt, C, Fiedler, I, Dietl, G, Ender, K 2000. Myogenesis and postnatal skeletal muscle cell growth as influenced by selection. Livestock Production Science 66, 177–188.Google Scholar
Rehfeldt, C, Tuchscherer, A, Hartung, M, Kuhn, G 2008a. A second look at the influence of birth weight on carcass and meat quality in pigs. Meat Science 78, 170–175.Google Scholar
Rehfeldt, C, Henning, M, Fiedler, I 2008b. Consequences of pig domestication for skeletal muscle growth and cellularity. Livestock Science 116, 30–41.Google Scholar
Rehfeldt, C, Stabenow, B, Pfuhl, R, Block, J, Nürnberg, G, Otten, W, Metges, CC, Kalbe, C 2012. Effects of limited and excess protein intakes of pregnant gilts on carcass quality and cellular properties of skeletal muscle and subcutaneous adipose tissue in fattening pigs. Journal of Animal Science 90, 184–196.Google Scholar
Wank, V, Fischer, MS, Walter, B, Bauer, R 2006. Muscle growth and fiber type composition in hind limb muscles during postnatal development in pigs. Cells Tissues Organs 182, 171–181.CrossRefGoogle ScholarPubMed
Wigmore, PM, Stickland, NC 1983. Muscle development in large and small pig fetuses. Journal of Anatomy 137, 235–245.Google Scholar
Woodworth, JC, Tokach, MD, Nelssen, JL, Goodband, RD, Dritz, SS, Koo, SI, Minton, JE, Owen, KQ 2007. Influence of dietary l-carnitine and chromium picolinate on blood hormones and metabolites of gestating sows fed one meal per day. Journal of Animal Science 85, 2524–2537.Google Scholar
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