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Effects of dietary supplementation with grape seed procyanidins on nutrient utilisation and gut function in weaned piglets

Published online by Cambridge University Press:  07 October 2019

Q. H. Li ,
H. S. Yan ,
H. Q. Li ,
J. J. Gao  and
R. R. Hao Open the ORCID record for R. R. Hao [Opens in a new window]
Q. H. Li
Affiliation:
Department of Animal Production, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China Shanxi Collaborative Innovation Center for High-Productive and Safe Livestock, Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China
H. S. Yan
Affiliation:
Department of Animal Production, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China
H. Q. Li
Affiliation:
Shanxi Collaborative Innovation Center for High-Productive and Safe Livestock, Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China
J. J. Gao
Affiliation:
Library of Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China
R. R. Hao*
Affiliation:
Department of Animal Production, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China Shanxi Collaborative Innovation Center for High-Productive and Safe Livestock, Shanxi Agricultural University, Mingxian South Road 1th, Taigu 030801, PR China
*
†E-mail: [email protected]
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Abstract

Grape seed procyanidins (GSPs), widely known for their beneficial health properties, fail to bring about the expected improvement in piglets’ growth performance. The effects of dietary supplementation with GSPs on nutrient utilisation may be a critical influencing factor. Hence, the purpose of this study was to investigate the effects of dietary supplementation with GSPs on nutrient utilisation and gut function in weaned piglets. One hundred and twenty crossbred piglets were allocated randomly to four treatment groups, with three replicate pens per treatment and 10 piglets per pen. Each group was given one of the four dietary treatments: the basal diet (control group) or the basal diet with the addition of 50-, 100- or 150-mg/kg GSPs. The trial lasted 28 days. Faeces were collected from d 12 to 14 and from d 26 to 28 for measuring the coefficient of total tract apparent digestibility (CTTAD) of the nutrients. Blood samples were collected on d 14 and 28 for detecting the blood biochemical parameters. Two piglets per pen were slaughtered to collect the pancreas and intestinal digesta for evaluating the digestive enzyme activity and the coefficient of ileal apparent digestibility (CIAD) of the nutrients. On d 14 and 28, supplementation with 150-mg/kg GSPs significantly decreased the CTTAD of DM and CP in piglets. On d 14, GSPs supplementation at a concentration of 150 mg/kg led to a remarkable decrease in the CIAD of CP and gross energy (GE). On d 28, GSPs supplementation at a dose of 150 mg/kg generated a marked decline in the CIAD of DM, GE, CP and ether extract. Grape seed procyanidins supplementation at concentrations of 100 or 150 mg/kg inhibited the activities of lipase and amylase. In contrast, the jejunum mucosa maltase and sucrase activities increased due to the inclusion of GSPs at a concentration of 100 mg/kg in the piglet diet. Compared with the levels of the control group, the serum glucose and total protein levels were enhanced significantly by supplementation with GSPs at 100 mg/kg and reduced dramatically at 150 mg/kg. The serum diamine oxidase activity and endotoxin levels were decreased by GSPs supplementation in piglet diets. In conclusion, higher concentrations of GSPs in weaned piglet diets attenuated nutrient digestion and inhibited digestive enzyme activity; however, suitable concentrations of GSPs could promote brush-border enzyme activity, enhance serum glucose and total protein concentrations and decrease epithelial permeability.

Keywords

swinenutrient digestibilitydigestive enzyme activityblood biochemical parametersepithelial permeability
Type
Research Article
Information
animal , Volume 14 , Issue 3 , March 2020 , pp. 491 - 498
Copyright
© The Animal Consortium 2019 

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Footnotes

a

Co-first author.

References

Association of Official Analytical Chemists (AOAC) 2000. Official methods of analysis, 17th edition. AOAC, Gaithersburg, MD, USA.Google Scholar
Bibi, S, Kang, Y, Yang, G and Zhu, MJ 2016. Grape seed extract improves small intestinal health through suppressing inflammation and regulating alkaline phosphatase in IL-10-deficient mice. Journal of Functional Foods 20, 245–252.CrossRefGoogle Scholar
Che, L, Zhan, L, Fang, Z, Lin, Y, Yan, T and Wu, D 2012. Effects of dietary protein sources on growth performance and immune response of weanling pigs. Livestock Science 148, 1–9.CrossRefGoogle Scholar
Chu, H, Tang, Q, Huang, H, Hao, W and Wei, X 2016. Grape-seed proanthocyanidins inhibit the lipopolysaccharide-induced inflammatory mediator expression in RAW264.7 macrophages by suppressing MAPK and NF-κb signal pathways. Environmental Toxicology and Pharmacology 41, 159–166.CrossRefGoogle ScholarPubMed
Dinh, J, Angeloni, JT, Pederson, DB, Wang, X, Cao, M and Dong, Y 2014. Cranberry extract standardized for proanthocyanidins promotes the immune response of Caenorhabditis elegans to Vibrio cholerae through the p38 MAPK pathway and HSF-1. PLoS ONE 9, e103290.CrossRefGoogle ScholarPubMed
Dong, GZ and Pluske, JR 2007. The low feed intake in newly-weaned pigs: problems and possible solutions. Asian-Australasian Journal of Animal Sciences 20, 440–452.CrossRefGoogle Scholar
Goncalves, R, Mateus, N and De Freitas, V 2011a. Influence of carbohydrates on the interaction of procyanidin B3 with trypsin. Journal of Agricultural and Food Chemistry 59, 11794–11802.CrossRefGoogle ScholarPubMed
Goncalves, R, Mateus, N and De Freitas, V 2011b. Inhibition of α-amylase activity by condensed tannins. Food Chemistry 125, 665–672.CrossRefGoogle Scholar
Guil-Guerrero, JL, Ramos, L, Moreno, C, Zúñiga-Paredes, JC, Carlosama-Yépez, M and Ruales, P 2016. Plant-food by-products to improve farm-animal health. Animal Feed Science and Technology 220, 121–135.CrossRefGoogle Scholar
Hao, RR, Li, QH, Zhao, JQ, Li, HF, Wang, WW and Gao, JJ 2015. Effects of grape seed procyanidins on growth performance, immune function and antioxidant capacity in weaned piglets. Livestock Science 178, 237–242.CrossRefGoogle Scholar
Jakobek, L 2015. Interactions of polyphenols with carbohydrates, lipids and proteins. Food Chemistry 175, 556–567.CrossRefGoogle ScholarPubMed
Jayaraman, B and Nyachoti, CM 2017. Husbandry practices and gut health outcomes in weaned piglets: a review. Animal Nutrition 3, 205–211.CrossRefGoogle ScholarPubMed
Kosińska, A and Andlauer, W 2013. Modulation of tight junction integrity by food components. Food Research International 54, 951–960.CrossRefGoogle Scholar
Lau, DW and King, AJ 2003. Pre- and post-mortem use of grape seed extract in dark poultry meat to inhibit development of thiobarbituric acid reactive substances. Journal of Agricultural and Food Chemistry 51, 1602–1607.CrossRefGoogle ScholarPubMed
Li, LL, Yin, FG, Zhang, B, Peng, HZ, Li, FN, Zhu, NS, Hou, DX, Yin, YL, Luo, JJ, Tang, ZR and Liu, G 2011. Dietary supplementation with Atractylodes Macrophala Koidz polysaccharides ameliorate metabolic status and improve immune function in early-weaned pigs. Livestock Science 142, 33–41.CrossRefGoogle Scholar
Nabuurs, MJ, Hoogendoorn, A, van der Molen, EJ and van Osta, AL 1993. Villus height and crypt depth in weanead and unweanead pigs, reared under various circumstances in the Netherlands. Research in Veterinary Science 55, 78–84.CrossRefGoogle ScholarPubMed
National Research Council (NRC) 2012. Nutrient requirements of swine, 11th revised edition. National Academy Press, Washington, DC, USA.Google Scholar
Obreque-Slier, E, Peña-Neira, Á and López-Solís, R 2012. Interactions of enological tannins with the protein fraction of saliva and astringency perception are affected by pH. LWT-Food Science and Technology 45, 88–93.Google Scholar
Park, JS, Park, MK, Oh, HJ, Woo, YJ, Lim, MA, Lee, JH, Ju, JH, Jung, YO, Lee, ZH, Park, SH, Kim, HY, Cho, ML and Min, JK 2012. Grape-seed proanthocyanidin extract as suppressors of bone destruction in inflammatory autoimmune arthritis. PLoS ONE 7, e51377.CrossRefGoogle ScholarPubMed
Perez-Gregorio, MR, Mateus, N and de Freitas, V 2014. Rapid screening and identification of new soluble tannin-salivary protein aggregates in saliva by mass spectrometry (MALDI-TOF-TOF and FIA-ESI-MS). Langmuir 30, 8528–8537.CrossRefGoogle Scholar
Petzke, KJ, Schuppe, S, Rohn, S, Rawel, HM and Kroll, J 2005. Chlorogenic acid moderately decreases the quality of whey proteins in rats. Journal of Agricultural and Food Chemistry 53, 3714–3720.CrossRefGoogle ScholarPubMed
Rababah, TM, Ereifej, KI, Al-Mahasneh, MA and Al-Rababah, MA 2006. Effect of plant extracts on physicochemical properties of chicken breast meat cooked using conventional electric oven or microwave. Poultry Science 85, 148–154.CrossRefGoogle ScholarPubMed
Rawel, HM, Czajka, D, Rohn, S and Kroll, J 2002. Interactions of different phenolic acids and flavonoids with soy proteins. International Journal of Biological Macromolecules 30, 137–150.CrossRefGoogle ScholarPubMed
Shi, J, Yu, J, Pohorly, JE and Kakuda, Y 2003. Polyphenolics in grape seeds-biochemistry and functionality. Journal of Medicinal Food 6, 291–299.CrossRefGoogle ScholarPubMed
Shishikura, Y, Khokhar, S and Murray, BS 2006. Effects of tea polyphenols on emulsification of olive oil in a small intestine model system. Journal of Agricultural and Food Chemistry 54, 1906–1913.CrossRefGoogle Scholar
Shpigelman, A, Israeli, G and Livney, YD 2010. Thermally-induced protein-polyphenol co-assemblies: beta lactoglobulin-based nanocomplexes as protective nanovehicles for EGCG. Food Hydrocolloids 24, 735–743.CrossRefGoogle Scholar
Song, P, Zhang, R, Wang, X, He, P, Tan, L and Ma, X 2011. Dietary grape-seed procyanidins decreased postweaning diarrhea by modulating intestinal permeability and suppressing oxidative stress in rats. Journal of Agricultural and Food Chemistry 59, 6227–6232.CrossRefGoogle ScholarPubMed
Stojadinovic, M, Radosavljevic, J, Ognjenovic, J, Vesic, J, Prodic, I, Stanic-Vucinic, D and Cirkovic Velickovic, T 2013. Binding affinity between dietary polyphenols and β-lactoglobulin negatively correlates with the protein susceptibility to digestion and total antioxidant activity of complexes formed. Food Chemistry 136, 1263–1271.CrossRefGoogle ScholarPubMed
Sugiyama, H, Akazome, Y, Shoji, T, Yamaguchi, A, Yasue, M, Kanda, T and Ohtake, Y 2007. Oligomeric procyanidins in apple polyphenol are main active components for inhibition of pancreatic lipase and triglyceride absorption. Journal of Agricultural and Food Chemistry 55, 4604–4609.CrossRefGoogle ScholarPubMed
Terra, X, Pallarés, V, Ardèvol, A, Bladé, C, Fernández-Larrea, J, Pujadas, G, Salvadó, J, Arola, L and Blay, M 2011. Modulatory effect of grape-seed procyanidins on local and systemic inflammation in diet-induced obesity rats. The Journal of Nutritional Biochemistry 22, 380–387.CrossRefGoogle ScholarPubMed
Tomás-Barberán, FA and Andrés-Lacueva, C 2012. Polyphenols and health: current state and progress. Journal of Agricultural and Food Chemistry 60, 8773–8775.CrossRefGoogle ScholarPubMed
Uchiyama, S, Taniguchi, Y, Saka, A, Yoshida, A and Yajima, H 2011. Prevention of diet-induced obesity by dietary black tea polyphenols extract in vitro and in vivo. Nutrition 27, 287–292.CrossRefGoogle ScholarPubMed
Wijtten, PJ, van der Meulen, J and Verstegen, MW 2011. Intestinal barrier function and absorption in pigs after weaning: a review. British Journal of Nutrition 105, 967–981.CrossRefGoogle ScholarPubMed
Yang, G, Xue, Y, Zhang, H, Du, M and Zhu, MJ 2015. Favourable effects of grape seed extract on intestinal epithelial differentiation and barrier function in IL10-deficient mice. The British Journal of Nutrition 114, 15–23.CrossRefGoogle ScholarPubMed
Yuksel, Z, Avci, E and Erdem, YK 2010. Characterization of binding interactions between green tea flavanoids and milk proteins. Food Chemistry 121, 450–456.CrossRefGoogle Scholar
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