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Effect of mild heat stress and mild infection pressure on immune responses to an E. coli infection in chickens

Published online by Cambridge University Press:  01 February 2008

L. R. Norup ,
K. H. Jensen ,
E. Jørgensen ,
P. Sørensen  and
H. R. Juul-Madsen
L. R. Norup*
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, Research Centre Foulum, University of Aarhus, PO Box 50, DK-8830 Tjele, Denmark
K. H. Jensen
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, Research Centre Foulum, University of Aarhus, PO Box 50, DK-8830 Tjele, Denmark
E. Jørgensen
Affiliation:
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Research Centre Foulum, University of Aarhus, PO Box 50, DK-8830 Tjele, Denmark
P. Sørensen
Affiliation:
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Research Centre Foulum, University of Aarhus, PO Box 50, DK-8830 Tjele, Denmark
H. R. Juul-Madsen
Affiliation:
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, Research Centre Foulum, University of Aarhus, PO Box 50, DK-8830 Tjele, Denmark
*
E-mail: [email protected]
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Abstract

Outdoor or organic farming demands robust chickens that are able to combat common infections before they spread to the flock. Priming the immune system of the chickens early in life with micro-organisms that they will encounter later in life prepares chickens to a life in environments where they are subjected to a more natural level of infection pressure. Also, exposure to non-infectious stressful situations may prepare the immune system to combat infectious challenges. The present study investigated whether the immune system could be primed by applying small doses of infective material to the chicken flock or by exposure to short-term non-infectious stimulation, and whether the effect of those stimuli would depend on the genetic material chosen. The effect of the stimulations was examined on selected immunological variables in two chicken strains, using small amounts of manure and litter from other chickens or short-term heat stress, respectively. After 6 weeks of treatment, all chickens were subjected to an Escherichia coli infection and followed for another 3 weeks. Measures of body weight gain, chicken mannan-binding lectin (cMBL), percentage of CD4+ and MHCII+ lymphocytes, mean fluorescence intensity (m.f.i.) of CD4 on CD4+ cells and MHCII on MHCII+ cells and antibody titres to E. coli were taken. In conclusion, the chickens redistribute lymphocyte populations in peripheral blood in response to potentially infectious agents as well as to stressful non-infectious treatments. Responses to stress situations were dependent on the frequencies of stress exposures and on the chicken breed. This may reflect the superiority of one breed over another in adapting to treatments or in discriminating whether a treatment is harmless or dangerous. However, the differences did not influence the disease resistance to infection with a mixture of E. coli O2, O11 and O78 in the present study.

Keywords

chickensE. coliheatstressimmune response
Type
Full Paper
Information
animal , Volume 2 , Issue 2 , February 2008 , pp. 265 - 274
Copyright
Copyright © The Animal Consortium 2008

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References

Dhabhar, FS, Viswanathan, K 2005. Short-term stress experienced at time of immunization induces a long-lasting increase in immunologic memory. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 289, R738–R744.CrossRefGoogle ScholarPubMed
El-Lethey, H, Huber-Eicher, B, Jungi, TW 2003. Exploration of stress-induced immunosuppression in chickens reveals both stress-resistant and stress susceptible antigen responses. Veterinary Immunology and Immunopathology 95, 91–101.CrossRefGoogle ScholarPubMed
Gross, WB, Siegel, HS 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Diseases 27, 972–979.CrossRefGoogle ScholarPubMed
Hangalapura, BN, Nieuwland, MG, de Vries Reilingh, G, Heetkamp, MJ, Van den Brand, H, Kemp, B, Parmentier, HK 2003. Effects of cold stress on immune responses and body weight of chicken lines divergently selected for antibody responses to sheep red blood cells. Poultry Science 82, 1692–1700.CrossRefGoogle ScholarPubMed
Hangalapura, BN, Nieuwland, MG, de Vries Reilingh, G, Van den Brand, H, Kemp, B, Parmentier, HK 2004. Durations of cold stress modulates overall immunity of chicken lines divergently selected for antibody responses. Poultry Science 83, 765–775.CrossRefGoogle ScholarPubMed
Hangalapura, BN, Kaiser, MG, Poel, JJ, Parmentier, HK, Lamont, S 2006. Cold stress equally enhances in vivo pro-inflammatory cytokine gene expression in chicken lines divergently selected for antibody responses. Developmental and Comparative Immunology 30, 503–511.CrossRefGoogle ScholarPubMed
Juul-Madsen HR, Handberg KJ, Norup LR and Jørgensen PH 2002. The acute phase response of mannan-binding lectin (MBL) in chicken in relation to infectious diseases. Proceedings and Annual Report Cost Action 839 ‘Immunosuppressive viral diseases in poultry’, 126–129.Google Scholar
Juul-Madsen, HR, Munch, M, Handberg, KJ, Sørensen, P, Johnson, AA, Norup, LR, Jørgensen, PH 2003. Serum levels of mannan-binding lectin (MBL) in chickens prior to and during experimental infection with avian infectious bronchitis virus (IBV). Poultry Science 82, 235–241.CrossRefGoogle ScholarPubMed
Juul-Madsen HR, Handberg KJ, Norup LR and Jørgensen PH 2004. Natural resistance as an alternative to AGP in poultry. Danish Institute of Animal Science, Report, Animal Husbandry no. 57: Beyond Antimicrobial Growth Promoters in Food Animal Production, pp. 185–190.Google Scholar
Laursen, SB, Nielsen, OL 2000. Mannan-binding lectin (MBL) in chickens: molecular and functional aspects. Developmental and Comparative Immunology 24, 85–101.CrossRefGoogle ScholarPubMed
Mujahid, A, Sato, K, Akiba, Y, Toyomizu, M 2006. Acute heat stress stimulates mitochondrial superoxide production in broiler skeletal muscle, possibly via downregulation of uncoupling protein content. Poultry Science 85, 1259–1265.CrossRefGoogle ScholarPubMed
Nielsen, OL, Jensenius, JC, Jørgensen, PH, Laursen, SB 1999. Serum levels of chicken mannan binding lectin (MBL) during virus infections; indication that chicken MBL is an acute phase reactant. Veterinary Immunology and Immunopathology 70, 309–316.CrossRefGoogle ScholarPubMed
Norup, LR, Juul-Madsen, HR 2007. An assay for measuring the mannan-binding lectin pathway of complement activation in chicken. Poultry Science 86, 2322–2326.CrossRefGoogle Scholar
Post, J, Rebel, JM, ter Huurne, AA 2003. Physiological effects of elevated plasma corticosterone concentrations in broiler chickens. An alternative means by which to assess the physiological effects of stress. Poultry Science 82, 1313–1318.CrossRefGoogle ScholarPubMed
Rook, GAW, Stanford, JL 1998. Give us this day our daily germs. Immunology Today 19, 113–116.CrossRefGoogle ScholarPubMed
Shang, S-Q, Chen, G-X, Shen, J, Yu, X-H, Wang, KY 2005. The binding of MBL to common bacteria in infectious diseases of children. Journal of Zhejiang University 6B, 53–56.CrossRefGoogle Scholar
Sørensen P, Danell B, Brenøe U and Tuiskula-Haivisto M 2004. A review on poultry breeding stock in the nordic countries. Report from Nordic Gene Bank Farm Animals.Google Scholar
Star L, Nieuwland MG, Kemp B and Parmentier HK 2006. Effect of stress on haemolytic complement activity in layer lines. Ninth Avian Immunology Research Group Meeting, October 21st–24th, Paris, p. 73.Google Scholar
Statistical Analysis Systems Institute 1996. SAS®, system for mixed models. SAS Institute Incorporated, Cary, NC, USA.Google Scholar
Trout, JM, Mashaly, MM 1994. The effects of adrenocorticotropic hormone and heat stress on the distribution of lymphocyte populations in immature male chickens. Poultry Science 73, 1694–1698.CrossRefGoogle ScholarPubMed
Viswanathan, K, Daugherty, C, Dhabhar, FD 2005. Stress as an endogenous adjuvant: augmentation of the immunization phase of cell-mediated immunity. International Immunology 17, 1059–1069.CrossRefGoogle ScholarPubMed