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Effect of functional oils on the immune response of broilers challenged with Eimeria spp.

Published online by Cambridge University Press:  08 April 2019

P. O. Moraes Open the ORCID record for P. O. Moraes [Opens in a new window] ,
I. Andretta Open the ORCID record for I. Andretta [Opens in a new window] ,
K. M. Cardinal ,
M. Ceron ,
L. Vilella ,
R. Borille ,
A. P. Frazzon ,
J. Frazzon ,
E. Santin  and
A. M. L. Ribeiro
P. O. Moraes*
Affiliation:
Department of Animal Science and Rural Development, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88036-020, Brazil
I. Andretta
Affiliation:
Department of Animal, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil
K. M. Cardinal
Affiliation:
Department of Animal, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil
M. Ceron
Affiliation:
Department of Animal, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil
L. Vilella
Affiliation:
Department of Animal, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil
R. Borille
Affiliation:
Department of Animal Science and Biology Science, Federal University of Santa Maria, Palmeira das Missões, Rio Grande do Sul 98300-00, Brazil
A. P. Frazzon
Affiliation:
Department of Microbiology, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil
J. Frazzon
Affiliation:
Food Science and Technology Institute (ICTA), Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil
E. Santin
Affiliation:
Department of Veterinary Medicine, Federal University of Paraná, Curitiba, Paraná 80035-050, Brazil
A. M. L. Ribeiro
Affiliation:
Department of Animal, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil
*
†E-mail: [email protected]
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Abstract

Infection with Eimeria sp. results in the activation of multiple facets of the host immune system; the use of phytogenics can modulate the inflammatory response and improve the performance of the challenged animal. The aim of this study was to evaluate the effect of a commercial blend of cashew nut shell liquid (CNSL) and castor oil on the immune response of broilers challenged with coccidiosis. A total of 864 one-day-old male chicks (Cobb 500) were randomly distributed into six treatment groups (8 pens/treatment and 18 chicks/pen) in a three-by-two factorial design with three additives: control (non-additive), 100 ppm of monensin or 0.15% CNSL–castor oil. Challenge status was determined twice at 14 days of age. Unchallenged birds were inoculated by gavage with oocysts sporulated with Eimeria tenella, Eimeria acervulina and Eimeria maxima. Although the positive control (non-additive and challenged) and CNSL–castor oil treatment groups exhibited similar variation in weight gain (ΔBWG) compared to unchallenged birds fed without additives, the variation observed in birds fed diets containing CNSL–castor oil was associated with a higher maintenance requirement and not feed efficiency. In the second week after infection, ΔBWG of the CNSL–castor oil treatment group did not significantly change compared to the other treatment groups. At days 7 and 14 post-challenge, there was a higher excretion of oocysts in the control group, whereas the CNSL–castor oil and monensin groups did not differ. The CNSL–castor oil group exhibited increased gene expression of interferon (IFN), interleukin 6 (IL-6) and tumor necrosis factor (TNF), while the control group exhibited increased expression of cyclooxygenase (COX) and IL-1. The heterophils/lymphocyte ratio was low for the monensin treatment group. The unchallenged birds that received monensin treatment presented higher gene expression of IFN, COX and IL-1 compared to the other treatments, while the CNSL–castor oil group exhibited reduced gene expression, except for TNF. The commercial blend of cashew nut liquid and castor oil modulated the inflammatory response against Eimeria spp. In the absence of the parasite, there was no stimulation of genes involved in the inflammatory response, demonstrating that the blend is an effective tool in specifically modulating the immune system of birds afflicted with coccidiosis.

Keywords

cashew nutcastor oilchallengecoccidiosisinterleukins
Type
Research Article
Information
animal , Volume 13 , Issue 10 , October 2019 , pp. 2190 - 2198
Copyright
© The Animal Consortium 2019 

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References

Allen, PC and Fetterer, R 2002. Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clinical Microbiology Reviews 15, 58–65.CrossRefGoogle Scholar
Applegate, T, Klose, V, Steiner, T, Ganner, A and Schatzmayr, G 2010. Probiotics and phytogenics for poultry: Myth or reality? Journal of Applied Poultry Research 19, 194–210.CrossRefGoogle Scholar
Bess, F, Favero, A, Vieira, SL and Torrent, J 2012. The effects of functional oils on broiler diets of varying energy levels. The Journal of Applied Poultry Research 21, 567–578.CrossRefGoogle Scholar
Brisbin, JT, Gong, J, Orouji, S, Esufali, J, Mallick, AI, Parvizi, P, Shewen, PE and Sharif, S 2011. Oral treatment of chickens with lactobacilli influences elicitation of immune responses. Clinical and Vaccine Immunology 18, 1447–1455.CrossRefGoogle ScholarPubMed
Chapman, HD, Jeffers, TK and Williams, RB 2010. Forty years of monensin for the control of coccidiosis in poultry. Poultry Science 89, 1788–1801.CrossRefGoogle ScholarPubMed
Chen, X, Zhang, Q and Applegate, TJ 2016. Impact of dietary branched chain amino acids concentration on broiler chicks during aflatoxicosis. Poultry Science 95, 1281–1289.CrossRefGoogle ScholarPubMed
Cornelissen, JB, Swinkels, WJ, Boersma, WA and Rebel, JM 2009. Host response to simultaneous infections with Eimeria acervulina, maxima and tenella: a cumulation of single responses. Veterinary Parasitology l 162, 58–66.CrossRefGoogle ScholarPubMed
Costa, CAF and Paiva, DP 2009. Cultivo in vivo, in vitro e diagnóstico específico de Eimeria spp. de Gallus Gallus, 1st edition. Embrapa Informação Tecnológica, Brasília, DF, Brasil.Google Scholar
Cox, CM, Sumners, LH, Kim, S, McElroy, AP, Bedford, MR and Dalloul, RA 2010. Immune responses to dietary beta-glucan in broiler chicks during an Eimeria challenge. Poultry Science 89, 2597–2607.CrossRefGoogle ScholarPubMed
Grenier, B, Dohnal, I, Shanmugasundaram, R, Eicher, SD, Selvaraj, RK, Schatzmayr, G and Applegate, TJ 2016. Susceptibility of broiler chickens to coccidiosis when fed subclinical doses of deoxynivalenol and fumonisins-special emphasis on the immunological response and the mycotoxin interaction. Toxins 8, 231–253.CrossRefGoogle ScholarPubMed
Hu, JL, Yu, H, Kulkarni, RR, Sharif, S, Xie, MY and Gong, J 2015. Modulation of cytokine gene expression by selected Lactobacillus isolates in the ileum, caecal tonsils and spleen of Salmonella-challenged broilers. Avian Pathology 44, 463–469.CrossRefGoogle ScholarPubMed
Iseri, V and Klasing, K 2014. Changes in the amount of lysine in protective proteins and immune cells after a systemic response to dead Escherichia coli: implications for the nutritional costs of immunity. Integrative and Comparative Biology 54, 922–930.CrossRefGoogle ScholarPubMed
Jiang, Z, Schatzmayr, G, Mohnl, M and Applegate, TJ 2010. Net effect of an acute phase response—partial alleviation with probiotic supplementation. Poultry Science 89, 28–33.CrossRefGoogle ScholarPubMed
Kim, J-Y, Park, SJ, Yun, K-J, Cho, Y-W, Park, H-J and Lee, K-T 2008. Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-κB in RAW 264.7 macrophages. European Journal of Pharmacology 584, 175–184.CrossRefGoogle ScholarPubMed
Klasing, KC 2004. The costs of immunity. Acta Zoologica Sinica 50, 961–969.Google Scholar
Klasing, K and Iseri, V 2013. Recent advances in understanding the interactions between nutrients and immunity in farm animals. In Proceedings of the 4th Energy and Protein Metabolism and Nutrition in Sustainable Animal Production, 9–12 September 2013, California, USA, pp. 353–359.CrossRefGoogle Scholar
Kogut, MH and Klasing, K 2009. An immunologist’s perspective on nutrition, immunity, and infectious diseases: introduction and overview. Journal of Applied Poultry Research 18, 103–110.CrossRefGoogle Scholar
Kraieski, AL, Hayashi, RM, Sanches, A, Almeida, GC and Santin, E 2016. Effect of aflatoxin experimental ingestion and Eimeria vaccine challenges on intestinal histopathology and immune cellular dynamic of broilers: applying an Intestinal Health Index. Poultry Science 96, 1078–1087.Google Scholar
Laurent, F, Mancassola, R, Lacroix, S, Menezes, R and Naciri, M 2001. Analysis of chicken mucosal immune response to Eimeria tenella and Eimeria maxima infection by quantitative reverse transcription-PCR. Infection and Immunity 69, 2527–2534.CrossRefGoogle ScholarPubMed
Lee, SH, Lillehoj, HS, Lillehoj, EP, Cho, SM, Park, DW, Hong, YH, Chun, HK and Park, HJ 2008. Immunomodulatory properties of dietary plum on coccidiosis. Comparative Immunology, Microbiology and Infectious Diseases 31, 389–402.CrossRefGoogle ScholarPubMed
Lee, KW, Lillehoj, HS, Lee, SH, Jang, SI, Park, MS, Bautista, DA, and Lillehoj, EP 2012. Effect of dietary antimicrobials on immune status in broiler chickens. Asian-Australasian Journal of Animal Sciences, 25, 392–392.CrossRefGoogle ScholarPubMed
Lillehoj, H and Choi, K 1998. Recombinant chicken interferon-gamma-mediated inhibition of Eimeria tenella development in vitro and reduction of oocyst production and body weight loss following Eimeria acervulina challenge infection. Avian Diseases 307–314.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402–408.CrossRefGoogle Scholar
Long, FY, Guo, YM, Wang, Z, Liu, D, Zhang, BK and Yang, X 2011. Conjugated linoleic acids alleviate infectious bursal disease virus-induced immunosuppression in broiler chickens. Poultry Science, 90, 1926–1933.CrossRefGoogle ScholarPubMed
Moraes, P, Gouvêa, F, Schroeder, B, Villela, L, Torrent, J and Ribeiro, A 2017. Effect of a commercial blend of cashew nut shell liquid and castor oil on the microbiota of broilers challenged with Eimeria spp. In Proceedings of the 106th Poultry Science Association Annual Meeting Conference, 17–20 July 2017, Orlando Florida, pp. 60.Google Scholar
Murakami, AE, Eyng, C and Torrent, J 2014. Effects of functional oils on coccidiosis and apparent metabolizable energy in broiler chickens. Asian-Australasian Journal of Animal Sciences 27, 981–989.CrossRefGoogle ScholarPubMed
Pastorelli, H, van Milgen, J, Lovatto, P and Montagne, L 2012. Meta-analysis of feed intake and growth responses of growing pigs after a sanitary challenge. Animal 6, 952–961.CrossRefGoogle ScholarPubMed
Rostagno, H, Ferreira, A, Barreto, S and Euclides, S R 2011. Tabelas brasileiras para aves e suínos, 4th revised edition. Universidade Federal de Viçosa, Viçosa, MG, Brasil.Google Scholar
Tan, J, Applegate, TJ, Liu, S, Guo, Y and Eicher, SD 2014. Supplemental dietary L-arginine attenuates intestinal mucosal disruption during a coccidial vaccine challenge in broiler chickens. British Journal of Nutrition 112, 1098–1109.CrossRefGoogle ScholarPubMed
SAS 2002. Proprietary software release 9.2. SAS Institute, Cary, NC, USA.Google Scholar
Tizard, I 2009. Imunologia Veterinária, 9th revised edition. Elsevier, Rio de Janeiro, RJ, Brazil.Google Scholar
Xie, J, Tian, C, Zhu, Y, Zhang, L, Lu, L and Luo, 2014. Effects of inorganic and organic manganese supplementation on gonadotropin-releasing hormone-I and follicle-stimulating hormone expression and reproductive performance of broiler breeder hens. Poultry Science 93, 959–969.CrossRefGoogle ScholarPubMed
Zhang, Q, Chen, X, Eicher, SD, Ajuwon, KM and Applegate, TJ 2016. Effect of threonine deficiency on intestinal integrity and immune response to feed withdrawal combined with coccidial vaccine challenge in broiler chicks. British Journal of Nutrition 116, 2030–2043.CrossRefGoogle ScholarPubMed
Zhou, Z-y, Hu, S-j, Wang, Z-y, Guo, Z-l, Qin, B and Nie, K 2014. Expression of chicken toll-like receptors and signal adaptors in spleen and cecum of young chickens infected with Eimeria tenella . Journal of Integrative Agriculture 13, 904–910.CrossRefGoogle Scholar
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