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Effect of dietary saponins from Quillaja saponaria L. on fatty acid composition and cholesterol content in muscle Longissimus dorsi of lambs

Published online by Cambridge University Press:  31 January 2011

D. M. R. Brogna ,
S. Nasri ,
H. Ben Salem ,
M. Mele ,
A. Serra ,
M. Bella ,
A. Priolo ,
H. P. S. Makkar  and
V. Vasta
D. M. R. Brogna
Affiliation:
University of Catania, Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, Via Valdisavoia 5, 95123, Catania, Italy
S. Nasri
Affiliation:
Laboratoire des Productiones Animales et Fourragères, Institut Nationale de la Recherche Agronomique de Tunisie (INRAT), Rue Hédi Karray, 2049 Ariana, Tunisia
H. Ben Salem
Affiliation:
Laboratoire des Productiones Animales et Fourragères, Institut Nationale de la Recherche Agronomique de Tunisie (INRAT), Rue Hédi Karray, 2049 Ariana, Tunisia
M. Mele
Affiliation:
Dipartimento di Agronomia e Gestione dell'Agro-Ecosistema, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy
A. Serra
Affiliation:
Dipartimento di Agronomia e Gestione dell'Agro-Ecosistema, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy
M. Bella
Affiliation:
University of Catania, Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, Via Valdisavoia 5, 95123, Catania, Italy
A. Priolo
Affiliation:
University of Catania, Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, Via Valdisavoia 5, 95123, Catania, Italy
H. P. S. Makkar
Affiliation:
Institute for Animal Production in the Tropics and Subtropics (480b), University of Hohenheim, D-70593 Stuttgart, Germany
V. Vasta*
Affiliation:
University of Catania, Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, Via Valdisavoia 5, 95123, Catania, Italy
*
†E-mail: [email protected]
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Abstract

The purpose of this study was to evaluate the effects of increasing levels of saponins from Quillaja saponaria on fatty acid (FA) composition and cholesterol content in muscle Longissimus dorsi of lambs. A total of 24 Barbarine lambs were assigned to four dietary treatments: control diet (C) consisting of oat hay ad libitum and 400 g of concentrate (80% barley, 17.5% soybean meal and 2.5% vitamin and mineral supplement); C diet plus 30 ppm of Q. saponaria L. (QS30); C diet plus 60 ppm of Quillaja (QS60); C diet plus 90 ppm of Quillaja (QS90). Saponin supplementation reduced the concentration of C14:1 cis-9 (P = 0.001) and of its desaturation index (P = 0.002). None of the FA intermediates of ruminal biohydrogenation (BH) was affected by Quillaja saponin supplementation (P > 0.05). The concentration of C20:4n-6 was higher in the meat of animals receiving 60 ppm of Quillaja than C and QS30 groups. Supplementing 60 ppm of Quillaja reduced the ratio between α-linolenic and linoleic acids compared with the C group (P = 0.023). We did not find any significant effect of Quillaja saponins on muscle cholesterol level. Further investigations are necessary to assess the metabolic fate of saponins in the rumen and to understand whether there is an effect of saponin on Δ9-desaturase enzyme activity, ruminal BH and cholesterol metabolism in ruminants. Supplementing up to 90 ppm of Quillaja saponins did not produce detrimental effects on the overall meat FA profile.

Keywords

saponinsfatty acid compositioncholesterolmeatlamb
Type
Full Paper
Information
animal , Volume 5 , Issue 7 , 31 May 2011 , pp. 1124 - 1130
Copyright
Copyright © The Animal Consortium 2011

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References

Abreu, A, Carulla, JE, Lascano, CE, Diaz, TE, Kreuzer, M, Hess, HD 2004. Effects of Sapindus saponaria fruits on ruminal fermentation and duodenal nitrogen flow of sheep fed a tropical grass diet with and without legume. Journal of Animal Science 82, 1392–1400.Google Scholar
Aurousseau, B, Bauchart, D, Calichon, E, Micol, D, Priolo, A 2004. Effect of grass or concentrate feeding systems and rate of growth on triglyceride and phospholipid and their fatty acids in the M. longissimus thoracis of lambs. Meat Science 66, 531–541.CrossRefGoogle ScholarPubMed
Boselli, E, Caboni, MF, Rodriguez-Estrada, MT, Toschi, TG, Daniel, M, Lercker, G 2005. Photoxidation of cholesterol and lipids of turkey meat during storage under commercial retail conditions. Food Chemistry 91, 705–713.Google Scholar
Brenner, RR 1989. Factors influencing fatty acid chain elongation and desaturation. In The role of fats in human nutrition (ed. AJ Vergroesen and M Crawford), pp. 45–80. Academic Press, California, USA.Google Scholar
Cheeke, PR 2000. Actual and potential applications of Quillaja saponaria and Quillaja saponaria saponins in human and animal nutrition. Journal of Animal Science 77, 1–10.Google Scholar
Choi, Y, Park, Y, Pariza, MW, Ntambi, JM 2001. Regulation of stearoyl-CoA desaturase activity by the trans-10, cis-12 isomer of conjugated linoleic acid in HepG2 cells. Biochemical and Biophysical Research Communications 284, 689–693.CrossRefGoogle ScholarPubMed
Christie, WW 1982. A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters. Journal of Lipid Research 23, 1072–1075.Google Scholar
Enoch, HG, Catalá, A, Strittmatter, P 1976. Mechanism of rat liver microsomal stearoyl-CoA desaturase. Journal of Biological Chemistry 251, 5095–5103.Google Scholar
Facino, RM, Carini, M, Stefani, R, Aldini, G, Saibene, L 2006. Anti-elastase and anti-hyaluronidase activities of saponins and sapogenins from Hedera helix, Aesculus hippocastanum, and Ruscus aculeatus: factors contributing to their efficacy in the treatment of venous insufficiency. Archiv der Pharmazie 328, 720–724.Google Scholar
Folch, J, Lees, M, Sloane Stanley, GH 1957. A simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Francis, G, Kerem, Z, Makkar, HPS, Becker, K 2002. The biological action of saponins in animal systems: a review. British Journal of Nutrition 88, 587–605.Google Scholar
Gutierrez, J, Davis, RE, Lindahl, IL 1959. Characteristics of saponin-utilizing bacteria from the rumen of cattle. Applied Microbiology 7, 304–308.Google Scholar
Harris, WS, Dujovne, CA, Windsor, SL, Gerrond, LLC, Newton, FA, Gelfand, RA 1997. Inhibiting cholesterol absorption with CP-88, 818 (beta-tigogenin cellobioside; tiqueside): studies in normal and hyperlipidemic subjects. Journal of Cardiovascular Pharmacology 30, 55–60.Google Scholar
Holtshausen, L, Chaves, AV, Beauchemin, KA, McGinn, SM, McAllister, TA, Odongo, NE, Cheeke, PR, Benchaar, C 2009. Feeding saponin-containing Yucca schidigera and Quillaja saponaria to decrease enteric methane production in dairy cows. Journal of Dairy Science 92, 2809–2821.Google Scholar
Kelsey, JA, Corl, BA, Collier, RJ, Bauman, DE 2003. The effect of breed, parity, and stage of lactation on conjugated linoleic acid (CLA) in milk fat from dairy cows. Journal of Dairy Science 86, 2588–2597.Google Scholar
Kemp, P, Lander, DJ 1984. Hydrogenation in vitro of alpha-linolenic acid to stearic acid by mixed cultures of pure strains of rumen bacteria. Journal of General Microbiology 130, 527–533.Google Scholar
Kepler, CR, Tove, SB 1967. Biohydrogenation of unsaturated fatty acids. 3. Purification and properties of a linoleate delta-12-cis, delta-11-trans-isomerase from Butyrivibrio fibrisolvens. Journal of Biological Chemistry 24, 5686–5692.CrossRefGoogle Scholar
Kim, HJ, Chun, YJ, Park, JD, Kim, SI, Roh, JK, Jeong, TC 1997. Protection of rat liver microsomes against carbon tetrachloride-induced lipid peroxidation by red ginseng saponin through cytochrome P450 inhibition. Planta Medica 63, 415–418.Google Scholar
Klita, PT, Mathison, GW, Fenton, TW, Hardin, RT 1996. Effects of alfalfa root saponins on digestive function in sheep. Journal of Animal Science 74, 1144–1156.CrossRefGoogle ScholarPubMed
Lu, CD, Jorgensen, NA 1987. Alfalfa saponins affect site and extent of nutrient digestion in ruminants. Journal of Nutrition 117, 919–927.Google Scholar
Makkar, HPS, Becker, K 1997. Degradation of Quillaja saponins by mixed culture of rumen microbes. Letters in Applied Microbiology 25, 243–245.Google Scholar
Makkar, HPS, Sen, S, Blummel, M, Becker, K 1998. Effects of fractions containing saponins from Yucca schidigera, Quillaja saponaria, and Acacia auriculoformis on rumen fermentation. Journal of Agricultural and Food Chemistry 46, 4324–4328.CrossRefGoogle Scholar
McGuire, MA, McGuire, MK 2000. Conjugated linoleic acid (CLA): a ruminant fatty acid with beneficial effects on human health. Journal of Animal Science 77, 1–8.Google Scholar
Mosley, EE, Powell, GL, Riley, MB, Jenkins, TC 2002. Microbial biohydrogenation of oleic acid to trans isomers in vitro. Journal of Lipid Research 43, 290–296.Google Scholar
Nasri, S, Ben Salem, H, Vasta, V, Abidi, A, Makkar, HPS, Priolo, A 2010. Effect of increasing levels of Quillaja saponaria on digestion, growth and meat quality of Barbarine lamb. Animal Feed Science and Technology, DOI: 10.1016/j.anifeedsci.2010.12.005CrossRefGoogle Scholar
Or-Rashid, MM, Odongo, NN, McBride, BW 2007. Fatty acid composition of ruminal bacteria and protozoa, with emphasis on conjugated linoleic acid, vaccenic acid, and odd-chain and branched-chain fatty acids. Journal of Animal Science 85, 1228–1234.CrossRefGoogle ScholarPubMed
Palmquist, DL, St-Pierre, N, McClure, KE 2004. Tissue fatty acid profiles can be used to quantify endogenous rumenic acid synthesis in lambs. Journal of Nutrition 134, 2407–2414.Google Scholar
Patra, AK, Saxena, J 2010. A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry 71, 1198–1222.Google Scholar
Potter, SM, Jimenez-Flores, R, Pollack, J, Lone, TA, Berber-Jimened, MD 1993. Protein-saponin interaction and its influence on blood lipids. Journal of Agricultural and Food Chemistry 41, 1287–1291.CrossRefGoogle Scholar
Sander, BD, Addis, PB, Park, SW, Smith, DE 1989. Quantification of cholesterol oxidation products in a variety of foods. Journal of Food Protection 52, 109–114.Google Scholar
Scaife, JR, Wahle, KW, Garton, GA 1978. Utilization of methylmalonate for the synthesis of branched-chain fatty acids by preparations of chicken liver and sheep adipose tissue. Biochemical Journal 176, 799–804.CrossRefGoogle ScholarPubMed
Sidhu, GS, Oakenfull, DG 1986. A mechanism for the hypocholesterolaemic activity of saponins. British Journal of Nutrition 55, 643–649.Google Scholar
Simopoulos, AP 1999. Essential fatty acids in health and chronic disease. American Journal of Clinical Nutrition 70 (suppl. 3), 560S–569S.CrossRefGoogle ScholarPubMed
Singer, MD, Robinson, PH, Salem, AZM, DePeters, EJ 2008. Impacts of rumen fluid modified by feeding Yucca schidigera to lactating dairy cows on in vitro gas production of 11 common dairy feedstuffs, as well as animal performance. Animal Feed Science and Technology 146, 242–258.CrossRefGoogle Scholar
Sweeley, CC, Bentley, R, Makita, M, Wells, W 1963. Gas-chromatography of trimethylsilyl derivatives of sugars and related substances. Journal of the American Chemical Society 85, 2497–2507.CrossRefGoogle Scholar
Vasta, V, Priolo, A, Scerra, M, Hallett, KG, Wood, JD, Doran, O 2009. Δ9-desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed herbage or concentrate with or without tannins. Meat Science 82, 357–364.CrossRefGoogle ScholarPubMed
Vlaeminck, B, Dewhurst, RJ, Demeyer, D, Fievez, V 2004. Odd and branched chain fatty acids to estimate proportions of cellulolytic and amylolytic particle associated bacteria. Journal of Animal and Feed Sciences 13, 235–238.Google Scholar
Wallace, RJ, Arthaud, L, Newbold, CJ 1994. Influence of Yucca shidigera extract on ruminal ammonia concentrations and ruminal microorganisms. Applied Environmental Microbiology 60, 1762–1767.CrossRefGoogle ScholarPubMed
Wang, Y, McAllister, TA, Yanke, LJ, Cheeke, PR 2000. Effect of steroidal saponin from Quillaja saponaria extract on ruminal microbes. Journal of Applied Microbiology 88, 887–896.CrossRefGoogle Scholar
Wang, Y, McAllister, TA, Newbold, CJ, Rode, LM, Cheeke, PR, Cheng, KJ 1998. Effects of Quillaja saponaria extract on fermentation and degradation of steroidal saponins in the rumen simulation technique (RUSITEC). Animal Feed Science and Technology 74, 143–153.CrossRefGoogle Scholar
Yoshiki, Y, Kudou, S, Okubo, K 1998. Relationship between chemical structures and biological activities of triterpenoid saponins from soybean (Review). Bioscience Biotechnology and Biochemistry 62, 2291–2299.CrossRefGoogle Scholar