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Effect of decreasing dietary protein level and replacing starch with soluble fibre on digestive physiology and performance of growing rabbits

Published online by Cambridge University Press:  28 February 2011

G. Xiccato ,
A. Trocino ,
D. Majolini ,
M. Fragkiadakis  and
M. Tazzoli
G. Xiccato*
Affiliation:
Department of Animal Science, University of Padova, Viale dell'Università 16, I-35020 Legnaro (Padova), Italy
A. Trocino
Affiliation:
Department of Animal Science, University of Padova, Viale dell'Università 16, I-35020 Legnaro (Padova), Italy
D. Majolini
Affiliation:
Department of Animal Science, University of Padova, Viale dell'Università 16, I-35020 Legnaro (Padova), Italy
M. Fragkiadakis
Affiliation:
Department of Animal Science, University of Padova, Viale dell'Università 16, I-35020 Legnaro (Padova), Italy
M. Tazzoli
Affiliation:
Department of Animal Science, University of Padova, Viale dell'Università 16, I-35020 Legnaro (Padova), Italy
*
†E-mail: [email protected]
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Abstract

At weaning (33 days of age), 246 hybrid rabbits (782 ± 53 g live weight) were divided into six experimental groups and fed ad libitum six iso-ADF diets formulated according to a bifactorial arrangement with two protein levels (152 and 162 g/kg) and three soluble fibre-to-starch ratios (0.2, 0.6 and 1.5), the latter obtained by replacing starch (from 209 to 91 g/kg) with soluble fibre (from 48 to 136 g/kg). The trial lasted for 42 days until slaughter. The rabbits that were fed the diet with the highest protein level and the lowest soluble fibre-to-starch ratio showed the highest mortality rate (17.1% v. 1.7% on average; P < 0.001) and sanitary risk (mortality + morbidity: 20.0% v. 8.1%; P = 0.04) compared with the rabbits fed the other diets. With increasing dietary crude protein level, the digestibility of dry matter (DM; 0.615 to 0.626) and gross energy (0.620 to 0.630) as well as aNDF (without sodium sulphite; 0.298 to 0.323) and hemicelluloses (0.417 to 0.461) significantly (0.001 < P < 0.10) improved. Moreover, total volatile fatty acids (VFAs) in the caecal content increased (59.0 to 68.4 mmol/l; P = 0.01) and ileum crypt depth tended to reduce (P = 0.07). Neither growth performance nor slaughter results were affected by the protein level. When increasing soluble fibre-to-starch ratio, the digestibility of DM and gross energy did not change, whereas the digestibility of aNDF (0.264 to 0.352), ADF (0.167 to 0.267) and hemicelluloses (0.400 to 0.470) linearly increased (P < 0.001). At caecum, N–ammonia tended to decrease linearly (P = 0.08), total VFA concentration (56.0 to 67.3 mmol/l) and acetate proportion (80.4 to 83.3 mmol/100 mmol VFA) linearly increased (P < 0.01), whereas butyrate and valerate proportions decreased (0.01 < P < 0.05). Growth performance was similar among groups, whereas at slaughter the proportion of the gastrointestinal tract linearly increased (177 to 184 g/kg; P < 0.01) without effect on dressing percentage, however. As soluble fibre-to-starch ratio increased, meat pH linearly decreased and lightness (L*), redness (a*) and yellowness (b*) colour indexes increased (0.01 < P < 0.05).

Keywords

proteinsoluble fibrestarchdigestive physiologygrowth performance
Type
Full Paper
Information
animal , Volume 5 , Issue 8 , August 2011 , pp. 1179 - 1187
Copyright
Copyright © The Animal Consortium 2011

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References

Álvarez, JL, Margüenda, I, García-Rebollar, P, Carabaño, R, De Blas, JC, Corujo, A, García-Ruiz, AI 2007. Effects of type and level of fibre on digestive physiology and performance in reproducing and growing rabbits. World Rabbit Science 15, 9–17.Google Scholar
Association of Official Analytical Chemists (AOAC) 2000. Official methods of analysis, vol. 1, 17th edition. AOAC, Arlington, VA, USA.Google Scholar
Blas, E, Gidenne, T 2010. Digestion of starch and sugars. In The nutrition of the rabbit, 2nd edition (ed. C De Blas and J Wiseman), pp. 19–38. CABI Publishing, Wallingford, UK.CrossRefGoogle Scholar
Blasco, A, Ouhayoun, J, Masoero, G 1993. Harmonization of criteria and terminology in rabbit meat research. World Rabbit Science 1, 3–10.Google Scholar
Carabaño, R, Motta-Ferreira, W, De Blas, C, Fraga, MJ 1997. Substitution of sugarbeet pulp for alfalfa hay in diets for growing rabbits. Animal Feed Science and Technology 65, 249–256.CrossRefGoogle Scholar
Carabaño, R, Villamide, MJ, García, J, Nicodemus, N, Llorente, A, Chamorro, S, Menoyo, D, García-Rebollar, P, García-Ruiz, AI, De Blas, JC 2009. New concepts and objectives for protein-amino acid nutrition in rabbits: a review. World Rabbit Science 17, 1–14.Google Scholar
Chamorro, S, Gómez-Conde, MS, Pérez de Rozas, AM, Badiola, I, Carabaño, R, De Blas, JC 2007. Effect on digestion and performance of dietary protein content and of increased substitution of lucerne hay with soya-bean protein concentrate in starter diets for young rabbits. Animal 1, 651–659.CrossRefGoogle ScholarPubMed
Chamorro, S, De Blas, C, Grant, G, Badiola, I, Menoyo, D, Carabaño, R 2010. Effect of dietary supplementation with glutamine and a combination of glutamine–arginine on intestinal health in twenty-five-day-old weaned rabbits. Journal of Animal Science 88, 170–180.CrossRefGoogle Scholar
Commission Internationale de l'Eclairage (CIE) 1976. Official recommendations on uniform colours spaces, colour differences equations and metric colours terms. Colorimetry, Paris, France.Google Scholar
De Blas, JC, Mateos, GG 2010. Feed formulation. In The nutrition of the rabbit, 2nd edition (ed. C De Blas and J Wiseman), pp. 222–232. CABI Publishing, Wallingford, UK.CrossRefGoogle Scholar
De Blas, JC, Pérez, E, Fraga, MJ, Rodríguez, JM, Gálvez, JF 1981. Effect of diet on feed intake and growth of rabbits from weaning to slaughter at different ages and weights. Journal of Animal Science 52, 1225–1232.CrossRefGoogle Scholar
European Community 1998. Commission Directive 98/64/EC of 3 September 1998 establishing Community methods of analysis for the determination of amino-acids, crude oils and fats, and olaquindox in feeding stuffs and amending Directive 71/393/EEC (L257). Official Journal of European Union, 14.Google Scholar
Falcão-e-Cunha, L, Peres, H, Freire, JPB, Castro-Solla, L 2004. Effects of alfalfa, wheat bran or beet pulp, with or without sunflower oil, on caecal fermentation and on digestibility in the rabbit. Animal Feed Science and Technology 117, 131–149.CrossRefGoogle Scholar
Feugier, A, Smit, MN, Fortun-Lamothe, L, Gidenne, T 2006. Fibre and protein requirements of early weaned rabbits and the interaction with weaning age: effects on digestive health and growth performance. Animal Science 82, 493–500.CrossRefGoogle Scholar
García, G, Galvez, JF, De Blas, JC 1993. Effect of substitution of sugar beet pulp for barley in diets for finishing rabbits on growth performance and on energy and nitrogen efficiency. Journal of Animal Science 71, 1823–1830.CrossRefGoogle Scholar
García, G, De Blas, JC, Carabaño, R, García, P 1995. Effect of type of lucerne hay on caecal fermentation and nitrogen contribution through caecotrophy in rabbits. Reproduction Nutrition Development 35, 267–275.CrossRefGoogle ScholarPubMed
García, J, Carabaño, R, Perez-Alba, L, De Blas, JC 2000. Effect of fibre source on caecal fermentation and nitrogen recycled through caecotrophy in rabbits. Journal of Animal Science 78, 638–646.CrossRefGoogle Scholar
García-Palomares, J, Carabaño, R, García-Rebollar, P, De Blas, JC, Corujo, A, García-Ruiz, AI 2006. Effects of a dietary protein reduction and enzyme supplementation on growth performance in the fattening period. World Rabbit Science 14, 231–236.Google Scholar
Gidenne, T, Bellier, R 2000. Use of digestible fibre in replacement of available carbohydrates – effect on digestion, rate of passage and caecal fermentation pattern during the growth of the rabbit. Livestock Production Science 63, 141–152.CrossRefGoogle Scholar
Gidenne, T, Jehl, N 1996. Replacement of starch by digestible fibre in the feed for the growing rabbit. 1. Consequences for digestibility and rate of passage. Animal Feed Science and Technology 61, 183–192.CrossRefGoogle Scholar
Gidenne, T, Perez, JM 2000. Replacement of digestible fibre by starch in the diet of the growing rabbit. I. Effects on digestion, rate of passage and retention of nutrients. Annales des Zootechnie 49, 357–368.CrossRefGoogle Scholar
Gidenne, T, Pinheiro, V, Falcão-E-Cunha, L 2000. A comprehensive approach of the rabbit digestion: consequences of a reduction of dietary fibre supply. Livestock Production Science 64, 225–237.CrossRefGoogle Scholar
Gidenne, T, Kerdiles, V, Jehl, N, Arveux, P, Briens, C, Eckenfelder, B, Fortun, H, Montessuy, S, Muraz, G 2001. Effet d'une hausse du ratio fibres digestibles/protéines sur les performances zootechniques et l’état sanitaire du lapin en croissance: résultats préliminaires d'une étude multi-site. In Proceedings 9èmes Journées de la Recherche Cunicole, Paris, France, pp. 65–68.Google Scholar
Gómez-Conde, MS, Pérez de Rozas, A, Badiola, I, Pérez-Alba, L, de Blas, JC, Carabaño, R, García, J 2009. Effect of neutral detergent soluble fibre on digestion, intestinal microbiota and performance in twenty five-day-old weaned rabbits. Livestock Science 125, 192–198.CrossRefGoogle Scholar
Gómez-Conde, MS, García, J, Chamorro, S, Eiras, P, García-Rebollar, PG, Perez De Rozas, A, Badiola, I, De Blas, C, Carabaño, R 2007. Neutral detergent-soluble fiber improves gut barrier function twenty-five-day-old weaned rabbits. Journal of Animal Science 85, 3313–3321.CrossRefGoogle ScholarPubMed
Hall, MB 2003. Challenges with nonfiber carbohydrate methods. Journal of Animal Science 81, 3226–3232.CrossRefGoogle ScholarPubMed
Jehl, N, Gidenne, T 1996. Replacement of starch by digestible fibre in feed for the growing rabbit. 2. Consequences for microbial activity in the caecum and on incidence of digestive disorders. Animal Feed Science and Technology 61, 193–204.CrossRefGoogle Scholar
Maertens, L, Luzi, F, De Groote, G 1997. Effect of dietary protein and amino acids on the performance, carcass composition and N-excretion of growing rabbits. Annales de Zootechnie 46, 255–268.CrossRefGoogle Scholar
Mertens, DR 2002. Gravimetric determination of amylase-treated neutral detergent fibre in feeds with refluxing beakers or crucibles: collaborative study. Journal of AOAC International 85, 1217–1240.Google ScholarPubMed
Parigi Bini, R, Xiccato, G, Cinetto, M 1990. Influenza del contenuto di amido alimentare sulla produttività, sulla digeribilità e sulla composizione corporea di conigli in accrescimento. Zootecnica e Nutrizione Animale 16, 271–282.Google Scholar
Perez, JM, Gidenne, T, Bouvarel, I, Arveaux, P, Bourdillon, A, Briens, C, La Naour, J, Messager, B, Mirabito, L 2000. Replacement of digestible fibre by starch in the diet of growing rabbits. II. Effects on performances and mortality by diarrhoea. Annales de Zootechnie 49, 369–377.CrossRefGoogle Scholar
Perez, JM, Lebas, F, Gidenne, T, Maertens, L, Xiccato, G, Parigi Bini, R, Dalle Zotte, A, Cossu, ME, Carazzolo, A, Villamide, MJ, Carabaño, R, Fraga, MJ, Ramos, MA, Cervera, C, Blas, E, Fernández, J, Falcão-e-Cunha, L, Bengala Freire, J 1995. European reference method for in vivo determination of diet digestibility in rabbits. World Rabbit Science 3, 41–43.Google Scholar
Soler, MD, Blas, E, Cano, JL, Pascual, JJ, Cervera, C, Fernández-Carmona, J 2004. Effect of digestible fibre/starch ratio and animal fat level in diets around weaning on mortality rate of rabbits. In Proceedings 8th World Rabbit Congress, Puebla, Mexico, pp. 996–1001. Retrieved August 20, 2010, from http://world-rabbit-science.com/WRSA-Proceedings/Congress-2004-Puebla/Papers/Feeding-&-Nutrition/N-Soler.pdf.Google Scholar
Statistical Analysis System Institute Inc. (SAS) 1991. User's guide, statistics, version 6.03. Edition SAS Institute Inc., Cary, NC, USA.Google Scholar
Trocino, A, Xiccato, G, Sartori, A, Queaque, PI 2000. Feeding plans at different protein levels: effects on growth performance, meat quality and nitrogen excretion in rabbits. World Rabbit Science 8 (suppl. 1), 467–474.Google Scholar
Trocino, A, Fragkiadakis, M, Majolini, D, Carabaño, R, Xiccato, G 2011. Effect of the increase of dietary starch and soluble fibre on the digestive efficiency and growth performance of meat rabbits. Animal Feed Science and Technology (under evaluation).CrossRefGoogle Scholar
Trocino, A, Fragkiadakis, M, Radaelli, G, Xiccato, G 2010. Effect of dietary soluble fibre level and protein source on growth, digestion, caecal activity and health of fattening rabbits. World Rabbit Science 18, 199–210.CrossRefGoogle Scholar
Van Soest, PJ, Robertson, JB, Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.CrossRefGoogle ScholarPubMed
Xiccato, G, Trocino, A 2010. Energy and protein metabolism and requirements. In The nutrition of the rabbit, 2nd edition (ed. C De Blas and J Wiseman), pp. 83–118. CABI Publishing, Wallingford, UK.CrossRefGoogle Scholar
Xiccato, G, Trocino, A, Carraro, L, Fragkiadakis, M 2006. Digestible fibre-to-ADF ratio and protein concentration in diets for early-weaned rabbits. In Proceedings 3rd Rabbit Congress of America, Maringá, Brazil, Comm. 35, pp. 1–6.Google Scholar
Xiccato, G, Trocino, A, Carraro, L, Fragkiadakis, M, Majolini, D 2008. Digestible fibre to starch ratio and antibiotic treatment time in growing rabbits affected by epizootic rabbit enteropathy. In Proceedings 9th World Rabbit Congress, Verona, Italy, pp. 847–851. Retrieved August 20, 2010, from http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/N-Xiccato.pdf.Google Scholar