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Effect of Bromocryptine on hormones and milk secretion in Murrah buffaloes (Bubalus bubalis)

Published online by Cambridge University Press:  02 February 2010

J. Prasad  and
M. Singh
J. Prasad
Affiliation:
Defense Institute of Physiology and Allied Sciences (Defense Research and Development Organization), Timarpur, New Delhi, India
M. Singh*
Affiliation:
Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal, Haryana, India
*
†E-mail: [email protected]
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Abstract

An experiment was carried out on 10 advance pregnant Murrah buffaloes to determine the role of hormones in milk secretion around parturition. Experimental animals were administered with a single injection of bromocryptine, @ 100 μg/kg BW, for 5 days before expected calving, whereas control group buffaloes were injected with placebo injections. Blood samples collected before parturition (−5,−4,−3,−2,−1 days), on day of parturition (day-0) and on day 1, 2, 3, 4, 5, 10 and 15 post partum were analyzed for growth hormone (GH), insulin like growth factor-I (IGF-I) and prolactin (PRL) by radioimmunassay methods. Milk samples were collected daily for 5 days and on day 10 and 15 after parturition. Milk fat, protein, lactose, citric acid, non-esterified fatty acids (NEFAs) and somatic cell counts (SCCs) were determined in milk samples. Bromocryptine treatment significantly (P < 0.01) decreased pre partum PRL and increased GH levels (P < 0.01) on day of parturition in experimental buffaloes without influencing plasma IGF-I level. Milk yield was significantly lower (P < 0.01) in experimental than in control group. Further, effect of bromocryptine on milk yield was only for a week. Milk yield increased (P < 0.01) gradually and was similar to control group on day 15 post partum. Bromocryptine treatment significantly increased milk SCC (P < 0.01) and protein content (P < 0.01) but there was no effect of treatment on fat, lactose, citric acid, glucose, milk and plasma NEFA concentration. It was concluded that prepartum suppression of PRL by bromocryptine impairs milk secretion temporarily in ensuing lactation. The significant rise in GH level before parturition and on day of parturition suggests a role of it in milk secretion of buffaloes.

Keywords

bromocryptine, milk yield and composition, hormones, metabolites, buffaloes
Type
Full Paper
Information
animal , Volume 4 , Issue 5 , May 2010 , pp. 772 - 776
Copyright
Copyright © The Animal Consortium 2010

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References

Accorsi, PA, Pacioni, B, Pezzi, C, Forni, M, Flint, DJ, Seren, E 2002. Role of prolactin, growth hormone and insulin-like growth factor 1 in mammary gland involution in the dairy cow. Journal of Dairy Science 85, 507–513.CrossRefGoogle ScholarPubMed
Akers, RM 1985. Lactogenic hormones: binding sites, mammary growth, secretory cell differentiation and milk bio-synthesis in ruminants. Journal of Dairy Science 68, 501–519.Google Scholar
Bauman, DE 1992. Bovine somatotropin: review of an emerging animal technology. Journal of Dairy Science 75, 3432–3451.CrossRefGoogle ScholarPubMed
Flint, DJ, Knight, CH 1997. Interaction of prolactin and growth hormone in the regulation of mammary gland function and epithelial cell survival. Journal of Mammary Gland Biology and Neoplasia 2, 41–48.CrossRefGoogle ScholarPubMed
Flint, DJ, Tonner, E, Beathie, J, Panton, D 1992. Investigation of the mechanism of action of growth hormone in stimulating lactation in the rat. Journal of Endocrinology 134, 377–383.CrossRefGoogle ScholarPubMed
Forsyth, IA, Byatt, JC, Iley, S 1985. Hormone concentrations, mammary development and milk yield in goats given long term bromocryptine treatment in pregnancy. Journal of Endocrinology 104, 77–85.Google Scholar
Glimm, DR, Baracos, VE, Kennelly, JJ 1990. Molecular evidence for the presence of growth hormone receptors in the bovine mammary gland. Journal of Endocrinology 126, R5–R8.CrossRefGoogle ScholarPubMed
Hart, IC 1973. Effect of 2-bromo-α-ergocryptine on milk yield and the level of prolactin and growth hormone in the blood of the goat at milking. Journal of Endocrinology 57, 179–180.CrossRefGoogle Scholar
Johke, T 1986. Prolactin secretion and lactogenesis in dairy cows and goats. Dairy Science Abstract 48, 7228.Google Scholar
Lee, PD, Forsyth, IA 1987. Milk yield in goats given short term bromocryptine treatment around parturition. Journal of Endocrinology 112 (suppl. 230), 988.Google Scholar
Ludri, RS, Upadhyay, RC, Singh, M, Guneratne, JRM, Basson, RP 1989. Milk production in lactating buffaloe receiving recombinantly produced bovine somatotropin (BST). Journal of Dairy Science 72, 2283–2287.Google Scholar
Marcek, JM, Swanson, LV 1984. Effect of photoperiod on milk production and prolactin of Holstein dairy cows. Journal of Dairy Science 67, 2380–2388.Google Scholar
Niswinder, GD 1974. Influence of 2-bromo-α-ergocryptine on serum levels of prolactin and the estrous cycle in sheep. Endocrinology 94, 612–615.Google Scholar
Pal, Yash 1996. Circulatory levels of some hormones and metabolites during initiation and early lactation in crossbred cows and buffaloes. PhD, National Dairy Research Institute, Deemed University, Karnal, Haryana, India.Google Scholar
Perry, NA, Doon, RJ 1950. Picric and method for simultaneous determination for lactose and sucrose in dairy products. Journal of Dairy Science 33, 176–180.Google Scholar
Saha, A, Singh, M 1998. Plasma hormones, blood metabolites, milk yield and composition in early lactation of buffaloes treated with bromocryptine. Asian-Australasian Journal of Animal Science 11, 368–374.Google Scholar
Schams, D, Reinhardt, V, Karg, H 1972. Effect of 2-Br-α-ergocryptine on plasma prolactin levels during parturition and onset of lactation in cows. Experientia 28, 697–699.Google Scholar
Shailja, C, Singh, M 2002. Post milking teat dip effect on somatic cell count, milk production and composition in cows and buffaloes. Asian-Australasian Journal of Animal Science 15, 1517–1522.Google Scholar
Shipe, WF, Senyk, GF, Fountain, KB 1980. Modified copper soap solvent extraction method for measuring free fatty acids in milk. Journal of Dairy Science 63, 193–198.Google Scholar
Singh, M, Dang, AK, Aggarwal, A 2002. Plasma insulin like growth factor-I levels in Murrah buffaloes (Bubalus bubalis) during lactation. Indian Journal of Dairy Science 55, 342–345.Google Scholar
Singh, M, Ludri, RS 1994. Plasma growth hormone profile and milk yield response of Murrah buffaloes (Bubalus bubalis) treated with slow release formulation of somidobone. Buffalo Journal 10, 81–84.Google Scholar
Singh, M, Ludri, RS 1999. Plasma prolactin, blood metabolites and yield and composition of milk during early lactation in goats following administration of bromocryptine. Asian-Australasian Journal of Animal Science 12, 585–589.Google Scholar
Singh, M, Ludri, RS 2000. Plasma prolactin, blood metabolites and milk production in bromocryptine-treated crossbred goats. Small Ruminant Research 35, 255–262.CrossRefGoogle Scholar
Singh, M, Ludri, RS 2001. Influence of stages of lactation, parity and season on somatic cell counts in cows. Asian-Australasian Journal of Animal Science 14, 1775–1780.Google Scholar
Singhal, OP, Desraj, 1989. New approaches for chemical quality assurance. Indian Dairyman 41, 43–47.Google Scholar
Snedecor, GW, Cochran, WC 1980. Statistical methods, 7th edition. Iowa State University Press, IO, USA, pp. 45–85.Google Scholar
White, JCD, Davies, DT 1963. The determination of citric acid in milk and milk sera. Journal of Dairy Research 30, 171–189.Google Scholar