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Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Published online by Cambridge University Press:  01 March 2009

B. Thiyagarajan  and
K. Valivittan
B. Thiyagarajan
Affiliation:
Department of Zoology, Presidency College, Chennai 600 005, Tamilnadu, India
K. Valivittan*
Affiliation:
Department of Zoology, Presidency College, Chennai 600 005, Tamilnadu, India
*
E-mail: [email protected]
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Abstract

Vitamin A is a well-known antioxidant and is essential for embryonic development, growth and differentiation. Oxidative stress is involved in the etiology of defective embryo development. The present study evaluated whether the presence of all-trans retinol (0, 1, 2, 5 and 10 μM) in maturation medium or embryo culture medium would enhance the developmental competence of preimplantation buffalo embryos in vitro. In experiment I, cumulus oocytes complex were matured with varying concentrations of all-trans retinol. Treatment with 5 μM all-trans retinol improved the blastocyst formation (P < 0.001) when compared with control and significant increase (P < 0.01) in total cell number was observed in 5 μM group when compared with control. Supplementation of all-trans retinol in embryo culture medium for the entire culture period under 5% O2 and 20% O2 was tested in experiments II and III, respectively. Supplementation of 10 μM all-trans retinol under 5% O2, significantly reduced blastocyst formation and cell numbers. Presence of 5 μM all-trans retinol under 20% O2 enhanced the frequency of blastocyst formation and total cell number (P < 0.001) when compared with control. DNA damage of individual embryos cultured under 20% oxygen concentration was measured by the comet assay. Supplementation of 5 μM all-trans retinol significantly reduced the comet tail (P < 0.001) when compared with control. Supplementation of all-trans retinol in embryo culture medium for first 72 h of the 8-day culture period under 5% O2 was tested in experiment IV. Addition of 5 μM all-trans retinol resulted in significant increase in blastocyst rate and total cell number (P < 0.001) when compared with control. Our results demonstrate that addition of all-trans retinol to maturation or embryo culture medium may enhance the developmental competence of buffalo embryos in vitro by enhancing blastocyst formation rate and total cell number.

Keywords

all-trans retinolblastocystembryo cultureoxidative stressoocytes
Type
Full Paper
Information
animal , Volume 3 , Issue 3 , March 2009 , pp. 385 - 392
Copyright
Copyright © The Animal Consortium 2008

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References

Ahlemeyer, B, Krieglstein, J 2000. Inhibition of glutathione depletion by retinoic acid and tocopherol protects cultured neurons from staurosporine-induced oxidative stress and apoptosis. Neurochemistry International 36, 1–5.CrossRefGoogle ScholarPubMed
Ahlemeyer, B, Bauerbach, E, Plath, M, Steuber, M, Heers, C, Tegtmeier, F, Krieglstein, J 2001. Retinoic acid reduces apoptosis and oxidative stress by preservation of SOD protein level. Free Radical Biology and Medicine 30, 1067–1077.CrossRefGoogle ScholarPubMed
Ali, AA, Bilodeau, JF, Sirard, MA 2003. Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development. Theriogenology 59, 939–949.CrossRefGoogle ScholarPubMed
Alminana, C, Gil, MA, Cuello, C, Caballero, I, Roca, J, Vazquez, JM, Gomez, E, Martinez, EA 2008. In vitro maturation of porcine oocytes with retinoids improves embryonic development. Reproduction Fertility and Development 20, 483–489.CrossRefGoogle ScholarPubMed
Brackett, BG, Oliphant, G 1975. Capacitation of rabbit spermatozoa in vitro. Biology of Reproduction 12, 260–274.CrossRefGoogle ScholarPubMed
Braude, P, Bolton, V, Moore, S 1988. Human gene expression first occurs between the four- and eight-cell stages of preimplantation development. Nature 332, 459–461.CrossRefGoogle ScholarPubMed
Brown, JA, Eberhardt, DM, Schrick, FN, Roberts, MP, Godkin, JD 2003. Expression of retinol-binding protein and cellular retinol-binding protein in the bovine ovary. Molecular Reproduction and Development 64, 261–269.CrossRefGoogle ScholarPubMed
Camous, S, Heyman, Y, Meziou, W, Menezo, Y 1984. Cleavage beyond the block stage and survival after transfer of early bovine embryos cultured with trophoblastic vesicles. Journal of Reproduction and Fertility 72, 479–485.CrossRefGoogle ScholarPubMed
Cetica, PD, Pintos, LN, Dalvit, GC, Beconi, MT 2001. Antioxidant enzyme activity and oxidative stress in bovine oocyte in vitro maturation. IUBMB Life 51, 57–64.CrossRefGoogle ScholarPubMed
Chow, CK 1979. Nutritional influence on cellular antioxidant defense systems. The American Journal of Clinical Nutrition 32, 1066–1081.CrossRefGoogle ScholarPubMed
Coskun, S, Lin, YC 1994. Effects of transforming growth factors and activin-A on in vitro porcine oocyte maturation. Molecular Reproduction and Development 38, 153–159.CrossRefGoogle ScholarPubMed
Drost, M, Wright, JM, and Elsden, RP 1986. Intergeneric embryo transfer between water buffalo and domestic cattle. Theriogenology 25, 13–23.CrossRefGoogle Scholar
Eberhardt, DM, Will, WA, Godkin, JD 1999. Retinol administration to superovulated ewes improves in vitro embryonic viability. Biology of Reproduction 60, 1483–1487.CrossRefGoogle ScholarPubMed
Gardiner, CS, Reed, DJ 1994. Status of glutathione during oxidant-induced oxidative stress in the preimplantation mouse embryo. Biology of Reproduction 51, 1307–1314.CrossRefGoogle ScholarPubMed
Gasparrini, B 2002. In vitro embryo production in buffalo species: state of the art. Theriogenology 57, 237–256.CrossRefGoogle ScholarPubMed
Gasparrini, B, Boccia, L, Marchandise, J, Di Palo, R, George, F, Donnay, I, Zicarelli, L 2006. Enrichment of in vitro maturation medium for buffalo (Bubalus bubalis) oocytes with thiol compounds: effects of cystine on glutathione synthesis and embryo development. Theriogenology 65, 275–287.CrossRefGoogle ScholarPubMed
Gilula, NB, Epstein, ML, Beers, WH 1978. Cell-to-cell communication and ovulation. A study of the cumulus-oocyte complex. The Journal of Cell Biology 78, 58–75.CrossRefGoogle ScholarPubMed
Goto, Y, Noda, Y, Mori, T, Nakano, M 1993. Increased generation of reactive oxygen species in embryos cultured in vitro. Free Radical Biology and Medicine 15, 69–75.CrossRefGoogle ScholarPubMed
Halliwell, B, Gutteridge, JM, Cross, CE 1992. Free radicals, antioxidants, and human disease: where are we now? The Journal of Laboratory and Clinical Medicine 119, 598–620.Google ScholarPubMed
Hidalgo, CO, Diez, C, Duque, P, Facal, N, Gomez, E 2003. Pregnancies and improved early embryonic development with bovine oocytes matured in vitro with 9-cis-retinoic acid. Reproduction 125, 409–416.CrossRefGoogle ScholarPubMed
Hossein, MS, Hashem, MA, Jeong, YW, Lee, MS, Kim, S, Kim, JH, Koo, OJ, Park, SM, Lee, EG, Park, SW, Kang, SK, Lee, BC, Hwang, WS 2007. Temporal effects of alpha-tocopherol and l-ascorbic acid on in vitro fertilized porcine embryo development. Animal Reproduction Science 100, 107–117.CrossRefGoogle ScholarPubMed
Ikeda, S, Kitagawa, M, Imai, H, Yamada, M 2005. The roles of vitamin A for cytoplasmic maturation of bovine oocytes. Journal of Reproduction and Development 51, 23–35.CrossRefGoogle ScholarPubMed
Johnson, MH, Nasr-Esfahani, MH 1994. Radical solutions and cultural problems: could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro? BioEssays 16, 31–38.CrossRefGoogle ScholarPubMed
Karaivanov, C 1986. Comparative studies on the superovulatory effect of PMSG and FSH in water buffalo (Bubalus bubalis). Theriogenology 26, 51–59.CrossRefGoogle ScholarPubMed
Kartha, VN, Krishnamurthy, S 1977. Antioxidant function of vitamin A. International Journal for Vitamin and Nutrition Research 47, 394–401.Google ScholarPubMed
Lima, PF, Oliveira, MA, Santos, MH, Reichenbach, HD, Weppert, M, Paula-Lopes, FF, Neto, CC, Goncalves, PB 2006. Effect of retinoids and growth factor on in vitro bovine embryos produced under chemically defined conditions. Animal Reproduction Science 95, 184–192.CrossRefGoogle ScholarPubMed
Livingston, T, Eberhardt, D, Edwards, JL, Godkin, J 2004. Retinol improves bovine embryonic development in vitro. Reproductive Biology and Endocrinology 2, 83–89.CrossRefGoogle ScholarPubMed
Lo, HW, Ali-Osman, F 1997. Genomic cloning of hGSTP1*C, an allelic human Pi class glutathione-S-transferase gene variant and functional characterization of its retinoic acid response elements. The Journal of Biological Chemistry 272, 32743–32749.CrossRefGoogle ScholarPubMed
Mermillod, P, Vansteenbrugge, A, Wils, C, Mourmeaux, JL, Massip, A, Dessy, F 1993. Characterization of the embryotrophic activity of exogenous protein-free oviduct-conditioned medium used in culture of cattle embryos. Biology of Reproduction 49, 582–587.CrossRefGoogle ScholarPubMed
Minegishi, T, Hirakawa, T, Kishi, H, Abe, K, Tano, M, Abe, Y, Miyamoto, K 2000. The mechanisms of retinoic acid-induced regulation on the follicle-stimulating hormone receptor in rat granulosa cells. Biochimica et Biophysica Acta 1495, 203–211.CrossRefGoogle ScholarPubMed
Mohan, M, Thirumalapura, NR, Malayer, J 2003. Bovine cumulus-granulosa cells contain biologically active retinoid receptors that can respond to retinoic acid. Reproductive Biology and Endocrinology 1, 104–112.CrossRefGoogle ScholarPubMed
Morris-Kay, GM, Ward, SJ 1999. Retinoids and mammalian development. International Review of Cytology 188, 73–131.CrossRefGoogle Scholar
Nasr-Esfahani, MH, Aitken, JR, Johnson, MH 1990. Hydrogen peroxide levels in mouse oocytes and early cleavage stage embryos developed in vitro or in vivo. Development 109, 501–507.CrossRefGoogle ScholarPubMed
Neglia, G, Gasparrini, B, Caracciolo di Brienza, V, Di Palo, R, Campanile, G, Antonio Presicce, G, Zicarelli, L 2003. Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration. Theriogenology 59, 1123–1130.CrossRefGoogle ScholarPubMed
Pawshe, CH, Totey, SM, Jain, SK 1994. A comparison of three methods of recovery of goat oocytes for in vitro maturation and fertilization. Theriogenology 42, 117–125.CrossRefGoogle Scholar
Sakkas, D, Batt, PA, Cameron, AW 1989. Development of preimplantation goat (Capra hircus) embryos in vivo and in vitro. Journal of Reproduction and Fertility 87, 359–365.CrossRefGoogle ScholarPubMed
Schweigert, FJ, Zucker, H 1988. Concentrations of vitamin A, beta-carotene and vitamin E in individual bovine follicles of different quality. Journal of Reproduction and Fertility 82, 575–579.CrossRefGoogle ScholarPubMed
Shaw, DW, Farin, PW, Washburn, SP, Britt, JH 1995. Effect of retinol palmitate on ovulation rate and embryo quality in superovulated cattle. Theriogenology 44, 51–58.CrossRefGoogle Scholar
Takahashi, M, Keicho, K, Takahashi, H, Ogawa, H, Schultz, RM, Okano, A 2000. Effect of oxidative stress on development and DNA damage in in-vitro cultured bovine embryos by comet assay. Theriogenology 54, 137–145.CrossRefGoogle ScholarPubMed
Tervit, HR, Whittingham, DG, Rowson, LE 1972. Successful culture in vitro of sheep and cattle ova. Journal of Reproduction and Fertility 30, 493–497.CrossRefGoogle ScholarPubMed
Totey, SM, Singh, G, Taneja, M, Pawshe, CH, Talwar, GP 1992. In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis). Journal of Reproduction and Fertility 95, 597–607.CrossRefGoogle ScholarPubMed
Xiao, JH, Durand, B, Chambon, P, Voorhees, JJ 1995. Endogenous retinoic acid receptor (RAR)-retinoid X receptor (RXR) heterodimers are the major functional forms regulating retinoid-responsive elements in adult human keratinocytes. Binding of ligands to RAR only is sufficient for RAR-RXR heterodimers to confer ligand-dependent activation of hRAR beta 2/RARE (DR5). The Journal of Biological Chemistry 270, 3001–3011.CrossRefGoogle ScholarPubMed