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Effect of adsorbants on in vitro biohydrogenation of 22:6n-3 by mixed cultures of rumen microorganisms

Published online by Cambridge University Press:  11 March 2016

M. Escobar ,
B. Vlaeminck ,
J. Jeyanathan ,
L. P. Thanh ,
K. J. Shingfield ,
R. J. Wallace  and
V. Fievez
M. Escobar
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
B. Vlaeminck
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
J. Jeyanathan
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
L. P. Thanh
Affiliation:
Department of Animal Sciences, Can Tho University, Can Tho 92100, Vietnam
K. J. Shingfield
Affiliation:
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth SY23 3FL, United Kingdom
R. J. Wallace
Affiliation:
Rowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, Aberdeen AB21 9SB, United Kingdom
V. Fievez*
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
*
† E-mail: [email protected]
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Abstract

Studies on microbial biohydrogenation of fatty acids in the rumen are of importance as this process lowers the availability of nutritionally beneficial unsaturated fatty acids for incorporation into meat and milk but also might result in the accumulation of biologically active intermediates. The impact was studied of adsorption of 22:6n-3 (DHA) to particulate material on its disappearance during 24 h in vitro batch incubations with rumen inoculum. Four adsorbants were used in two doses (1 and 5 mg/ml of mucin, gum arabic, bentonite or silicic acid). In addition, the distribution of 22:6n-3 in the pellet and supernatant of diluted rumen fluid was measured. Bentonite and silicic acid did not alter the distribution of 22:6n-3 between pellet and supernatant nor increased the disappearance of 22:6n-3 during the incubation. Both mucin and gum arabic increased the recovery of 22:6n-3 in the supernatant, indicating that these compounds lowered the adsorption of the fatty acid to ruminal particles. This was associated with an increased disappearance of 22:6n-3, when initial 22:6n-3 was 0.06 or 0.10 mg/ml, and an increased formation of 22:0, when initial 22:6n-3 was 0.02 mg/ml, during the 24 h batch culture experiment. Addition of gum arabic to pure cultures of Butyrivibrio fibrisolvens or Butyrivibrio proteoclasticus did not negate the inhibitory effect of 22:6n-3 on growth. As both mucin and gum arabic provide fermentable substrate for ruminal bacteria, an additional experiment was performed in which mucin and gum arabic were replaced by equal amounts of starch, cellulose or xylan. No differences in disappearance of 22:6n-3 were observed, suggesting that the stimulatory effect of mucin and gum arabic on disappearance of 22:6n-3 most probably is not due to provision of an alternative site of adsorption but related to stimulation of bacterial growth. A relatively high proportion of 22:6n-3 can be reduced to 22:0 provided the initial concentration is low.

Keywords

biohydrogenationrumenDHAadsorbants
Type
Research Article
Information
animal , Volume 10 , Supplement 9 , September 2016 , pp. 1439 - 1447
Copyright
© The Animal Consortium 2016 

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Footnotes

a

These authors contributed equally to this study.

References

AbuGhazaleh, AA and Jenkins, TC 2004. Disappearance of docosahexaenoic and eicosapentaenoic acids from cultures of mixed ruminal microorganisms. Journal of Dairy Science 87, 645–651.Google Scholar
Aldai, N, Hervas, G, Belenguer, A, Frutos, P, Mantecon, AR and Kramer, JK 2012. Evaluating the in vitro metabolism of docosahexaenoic acid in sheep rumen fluid. Lipids 47, 821–825.Google Scholar
Association of Official Analytical Chemists 1990. Acids (polyunsaturated) in oil and fats. In Official methods of analysis of the association of official analytical chemists (ed. , K and Helrich, ), pp. 960–963. Association of Official Analytical Chemists, Arlington, TX.Google Scholar
Bansil, R and Turner, BS 2006. Mucin structure, aggregation, physiological functions and biomedical applications. Current Opinion in Colloid and Interface Science 11, 164–170.CrossRefGoogle Scholar
Boeckaert, C, Vlaeminck, B, Fievez, V, Maignien, L, Dijkstra, J and Boon, N 2008. Accumulation of trans C18:1 fatty acids in the rumen after dietary algal supplementation is associated with changes in the Butyrivibrio community. Applied and Environmental Microbiology 74, 6923–6930.Google Scholar
Castro-Montoya, J, De Campeneere, S, Van Ranst, G and Fievez, V 2012. Interactions between methane mitigation additives and basal substrates on in vitro methane and VFA production. Animal Feed Science and Technology 176, 47–60.Google Scholar
Desbois, AP and Smith, VJ 2010. Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology 85, 1629–1642.CrossRefGoogle ScholarPubMed
De Weirdt, R, Coenen, E, Vlaeminck, B, Fievez, V, Van den Abbeele, P and Van de Wiele, T 2013. A simulated mucus layer protects Lactobacillus reuteri from the inhibitory effects of linoleic acid. Beneficial Microbes 4, 299–312.CrossRefGoogle ScholarPubMed
Fievez, V, Vlaeminck, B, Jenkins, T, Enjalbert, F and Doreau, M 2007. Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ. European Journal of Lipid Science and Technology 109, 740–756.CrossRefGoogle Scholar
Hassim, HA, Lourenco, M, Goel, G, Vlaeminck, B, Goh, YM and Fievez, V 2010. Effect of different inclusion levels of oil palm fronds on in vitro rumen fermentation pattern, fatty acid metabolism and apparent biohydrogenation of linoleic and linolenic acid. Animal Feed Science and Technology 162, 155–158.CrossRefGoogle Scholar
Jenkins, TC., Wallace, RJ, Moate, PJ and Mosley, EE 2008. Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Journal of Animal Science 86, 397–412.Google Scholar
Kairenius, P, Toivonen, V and Shingfield, KJ 2011. Identification and ruminal outflow of long-chain fatty acid biohydrogenation intermediates in cows fed diets containing fish oil. Lipids 46, 587–606.Google Scholar
Kenters, N, Henderson, G, Jeyanathan, J, Kittelmann, S and Janssen, PH 2011. Isolation of previously uncultured rumen bacteria by dilution to extinction using a new liquid culture medium. Journal of Microbiological Methods 84, 52–60.CrossRefGoogle ScholarPubMed
Kim, EJ, Huws, SA, Lee, MR, Wood, JD, Muetzel, SM, Wallace, RJ and Scollan, ND 2008. Fish oil increases the duodenal flow of long chain polyunsaturated fatty acids and trans-11 18:1 and decreases 18:0 in steers via changes in the rumen bacterial community. Journal of Nutrition 138, 889–896.Google Scholar
Klein, CM and Jenkins, TC 2011. Docosahexaenoic acid elevates trans-18:1 isomers but is not directly converted into trans-18:1 isomers in ruminal batch cultures. Journal of Dairy Science 94, 4676–4683.Google Scholar
Kramer, JKG, Hernandez, M, Cruz-Hernandez, C, Kraft, J and Dugan, MER 2008. Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2 and 18:3 except CLA isomers of milk fat as demonstrated using Ag-ion SPE fractionation. Lipids 43, 259–273.Google Scholar
Maia, MR, Chaudhary, LC, Figueres, L and Wallace, RJ 2007. Metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen. Antonie Van Leeuwenhoek 91, 303–314.CrossRefGoogle Scholar
Palatsi, J, Affes, R, Fernandez, B, Pereira, MA, Alves, MM and Flotats, X 2012. Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process. Water Research 46, 5268–5278.CrossRefGoogle ScholarPubMed
Pitt, GAJ and Morton, RA 1957. 9 Ultra-violet spectrophotometry of fatty acids. Progress in the Chemistry of Fats and Other Lipids 4, 227–278.Google Scholar
Proctor, DA and Palaniappan, S 1990. Adsorption of soy oil free fatty acids by rice hull ash. Journal of the American Oil Chemists Society 67, 15–17.Google Scholar
Rainio, A, Vahvaselkä, M, Suomalainen, T and Laakso, S 2001. Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp. shermanii . Canadian Journal of Microbiology 47, 735–740.Google Scholar
Rainio, A, Vahvaselkä, M and Laakso, S 2002. Cell-adhered conjugated linoleic acid regulates isomerization of linoleic acid by resting cells of Propionibacterium freudenreichii. Applied Microbiology and Biotechnology 60, 481–484.Google Scholar
Shingfield, KJ, Bonnet, M and Scollan, ND 2013. Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7, 132–162.Google Scholar
Shingfield, KJ, Kairenius, P, Arola, A, Paillard, D, Muetzel, S, Ahvenjarvi, S, Vanhatalo, A, Huhtanen, P, Toivonen, V, Griinari, JM and Wallace, RJ 2012. Dietary fish oil supplements modify ruminal biohydrogenation, alter the flow of fatty acids at the omasum, and induce changes in the ruminal Butyrivibrio population in lactating cows. Journal of Nutrition 142, 1437–1448.Google Scholar
Shingfield, KJ, Lee, MRF, Humphries, DJ, Scollan, ND, Toivonen, V, Beever, DE and Reynolds, CK 2011. Effect of linseed oil and fish oil alone or as an equal mixture on ruminal fatty acid metabolism in growing steers fed maize silage-based diets. Journal of Animal Science 89, 3728–3741.Google Scholar
Shingfield, KJ, Reynolds, CK, Hervas, G, Griinari, JM, Grandison, AS and Beever, DE. 2006. Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. Journal of Dairy Science 89, 714–732.Google Scholar
Van der Meer, JM 1985. CEC workshop on methodology of feedingstuffs for ruminants: European in vitro ringtest: manual. I.V.V.O., Lelystad.Google Scholar
Vlaeminck, B, Braeckman, T and Fievez, V 2014. Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition. Lipids 49, 517–525.Google Scholar
Vlaeminck, B, Mengistu, G, Fievez, V, de Jonge, L and Dijkstra, J 2008. Effect of in vitro docosahexaenoic acid supplementation to marine algae-adapted and unadapted rumen inoculum on the biohydrogenation of unsaturated fatty acids in freeze-dried grass. Journal of Dairy Science 91, 1122–1132.Google Scholar
Wasowska, I, Maia, MR, Niedzwiedzka, KM, Czauderna, M, Ribeiro, JM, Devillard, E, Shingfield, KJ and Wallace, RJ 2006. Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. British Journal of Nutrition 95, 1199–1211.Google Scholar
Xiang, S, Yao, X, Zhang, W, Zhang, K, Fang, Y, Nishinari, K, Phillips, GO and Jiang, F 2015. Gum arabic-stabilized conjugated linoleic acid emulsions: emulsion properties in relation to interfacial adsorption behaviors. Food Hydrocolloids 48, 110–116.Google Scholar
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