Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-14T03:26:15.548Z Has data issue: false hasContentIssue false
  • English
  • Français

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Published online by Cambridge University Press:  16 August 2010

J. L. Wu ,
W. Z. Liu ,
J. H. Liu ,
L. Y. Qiao  and
Y. N. Yuan
J. L. Wu
Affiliation:
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China
W. Z. Liu*
Affiliation:
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China
J. H. Liu
Affiliation:
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China
L. Y. Qiao
Affiliation:
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China
Y. N. Yuan
Affiliation:
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China
*
†E-mail: [email protected]
Get access

Abstract

The β-3 adrenergic receptor (ADRB3) is a G-protein coupled receptor involved in regulating lipolysis, as part of homeostatic regulation. In this study, South African Mutton Merino and Shanxi Dam Line were used to study the distribution and quantification of ADRB3 in adipose (subcutaneous, omental, retroperitoneal, mesenteric and perirenal fat) and non-adipose (heart, liver, spleen, lung and kidney) tissues of sheep. The protein was determined by immunohistochemical technique and by mRNA abundance via real-time polymerase chain reaction. ADRB3 was detected in all studied tissues with abundance in adipose tissues higher than in non-adipose tissues (P < 0.001). For adipose tissues, greater expression was found in deep deposits such as great omental and retroperitoneal fat than in subcutaneous fat (P < 0.05). Significant differences (P < 0.05) both for mRNA and for protein expression also existed between the two sheep flocks. These findings are consistent with the known function of ADRB3 in mediating lipolysis and homeostasis in adipose tissues.

Keywords

β-3 adrenergic receptorsheeptissuesmRNA expressionimmunohistochemistry
Type
Full Paper
Information
animal , Volume 5 , Issue 1 , January 2011 , pp. 88 - 93
Copyright
Copyright © The Animal Consortium 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anthony, A, Sim, R, Guillaume, JL, Strosberg, AD, Dhillon, AP, Pounder, RE, Wakefield, AJ 2000. Beta-3 adrenergic receptors in human pancreatic islet and duodenal somatostatin neuroendocrine cells. Alimentary Pharmacology and Therapeutics 14, 579–585.Google Scholar
Arch, JR 2008. The discovery of drugs for obesity, the metabolic effects of leptin and variable receptor pharmacology: perspectives from beta-3 adrenoceptor agonists. Naunyn-Schmiedeberg’s Archives of Pharmacology 378, 225–240.Google Scholar
Berkowitz, DE, Nardone, NA, Smiley, RM, Price, DT, Kreutter, DK, Fremeau, RT, Schwinn, DA 1995. Distribution of beta-3 adrenoceptor mRNA in human tissues. European Journal of Pharmacology 289, 223–228.Google Scholar
Cannon, B, Nedergaard, J 2004. Brown adipose tissue: function and physiological significance. Physiological Reviews 84, 277–359.Google Scholar
Carron, J, Morel, C, Hammon, HM, Blum, JW 2005. Ontogenetic development of mRNA levels and binding sites of hepatic beta-adrenergic receptors in cattle. Domestic Animal Endocrinology 28, 320–330.Google Scholar
Chamberlain, PD, Jennings, KH, Paul, F, Cordell, J, Berry, A, Holmes, SD, Park, J, Chambers, J, Sennitt, MV, Stock, MJ, Cawthorne, MA, Young, PW, Murphy, GJ 1999. The tissue distribution of the human β3-adrenoceptor studied using a monoclonal antibody: direct evidence of the b3-adrenoceptor in human adipose tissue, atrium and skeletal muscle. International Journal of Obesity 23, 1057–1065.Google Scholar
Cheng, HJ, Zhang, ZS, Onishi, K, Ukai, T, Sane, DC, Cheng, CP 2001. Upregulation of functional β3-adrenergic receptor in the failing canine myocardium. Circulation Research 89, 599–606.Google Scholar
De Matteis, R, Arch, JRS, Petroni, ML, Ferrari, D, Cinti, S, Stock, MJ 2002. Immunohistochemical identification of the β3-adrenoceptor in intact human adipocytes and ventricular myocardium: effect of obesity and treatment with ephedrine and caffeine. International Journal of Obesity 26, 1442–1450.Google Scholar
Evans, BA, Papaioannou, M, Bonazzi, VR, Summers, RJ 1996. Expression of beta 3-adrenoceptor mRNA in rat tissues. British Journal of Pharmacology 117, 210–216.Google Scholar
Igawa, Y, Yamazaki, Y, Takeda, H, Hayakawa, K, Akahane, M, Ajisawa, Y, Yoneyama, T, Nishizawa, O, Andersson, KE 1999. Functional and molecular biological evidence for a possible beta 3-adrenoceptor in the human detrusor muscle. British Journal of Pharmacology 126, 819–825.Google Scholar
Inderwies, T, Pfaffl, MW, Meyer, HH, Blum, JW, Bruckmaier, RM 2003. Detection and quantification of mRNA expression of alpha- and beta-adrenergic receptor subtypes in the mammary gland of dairy cows. Domestic Animal Endocrinology 24, 123–135.Google Scholar
Kobel, B, Engel, L, Ontsouka, EC, Graber, HU, Blum, JW, Steiner, A, Meylan, M 2006. Quantitative mRNA analysis of adrenergic receptor subtypes in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation. American Journal of Veterinary Research 67, 1367–1376.Google Scholar
Krief, S, Lönnqvist, F, Raimbault, S, Baude, B, Van Spronsen, A, Arner, P, Strosberg, AD, Ricquier, D, Emorine, LJ 1993. Tissue distribution of Beta 3-adrenergic receptor mRNA in man. The Journal of Clinical Investigation 91, 344–349.Google Scholar
Larsen, TM, Toubro, S, van Baak, MA, Gottesdiener, KM, Larson, P, Saris, WHM, Astrup, A 2002. Effect of a 28-d treatment with L-796568, a novel beta3-adrenergic receptor agonist, on energy expenditure and body composition in obese men. American Journal of Clinical Nutrition 76, 780–788.Google Scholar
Matos, LL, Stabenow, E, Tavares, MR, Ferraz, AR, Capelozzi, VL, Pinhal, MA 2006. Immunohistochemistry quantification by a digital computer-assisted method compared to semiquantitative analysis. Clinics 61, 417–424.CrossRefGoogle ScholarPubMed
McNeel, RL, Mersmann, HJ 1995. β3-adrenergic receptor subtypes transcripts in porcine adipose tissues. Journal of Animal Science 73, 1962–1971.CrossRefGoogle Scholar
McNeel, RL, Mersmann, HJ 1999. Distribution and quantification of beta1-, beta2-, and beta3-adrenergic receptor subtype transcripts in porcine tissues. Journal of Animal Science 77, 611–621.CrossRefGoogle ScholarPubMed
Meylan, M, Georgieva, TM, Reist, M, Blum, JW, Martig, J, Georgiev, IP, Steiner, A 2004. Distribution of mRNA that codes for subtypes of adrenergic receptors in the gastrointestinal tract of dairy cows. American Journal of Veterinary Research 65, 1142–1150.CrossRefGoogle ScholarPubMed
Moniotte, S, Kobzik, L, Feron, O, Trochu, JN, Gauthier, C, Balligand, JL 2001. Upregulation of β3-adrenoceptors and altered contractile response to inotropic amines in human failing myocardium. Circulation 103, 1649–1655.Google Scholar
Morimoto, A, Hasegawa, H, Cheng, HJ, Little, WC, Cheng, CP 2004. Endogenous beta3-adrenoreceptor activation contributes to left ventricular and cardiomyocyte dysfunction in heart failure. American Journal of Physiology Heart and Circulatory Physiology 286, H2425–H2433.CrossRefGoogle ScholarPubMed
Pietri-Rouxel, F, Lenzen, G, Kapoor, A, Drumare, MF, Archimbault, P, Strosberg, AD, Manning, BSJ 1995. Molecular cloning and pharmacological characterization of the bovine beta 3-adrenergic receptor. European Journal of Biochemistry 230, 350–358.Google Scholar
Rodriguez, M, Carillon, C, Coquerel, A, Le Fur, G, Ferrara, P, Caput, D, Shire, D 1995. Evidence for the presence of beta 3-adrenergic receptor mRNA in the human brain. Brain Research. Molecular Brain Research 29, 369–375.Google Scholar
Rouget, C, Barthez, O, Goirand, F, Leroy, MJ, Breuiller-Fouché, M, Rakotoniaina, Z, Guérard, P, Morcillo, EJ, Advenier, C, Sagot, P, Cabrol, D, Dumas, M, Bardou, M 2006. Stimulation of the ADRB3 adrenergic receptor induces relaxation of human placental arteries: influence of preeclampsia. Biology of Reproduction 74, 209–216.Google Scholar
Rozec, B, Gauthier, C 2006. Beta3-adrenoceptors in the cardiovascular system: putative roles in human pathologies. Pharmacology and Therapeutics 111, 652–673.Google Scholar
Rozec, B, Serpillon, S, Toumaniantz, G, Sèze, C, Rautureau, Y, Baron, O, Noireaud, J, Gauthier, C 2005. Characterization of beta3-Adrenoceptors in human internal mammary artery and putative involvement in coronary artery bypass management. Journal of the American College of Cardiology 46, 351–359.Google Scholar
Sasaki, N, Uchida, E, Niiyama, M, Yoshida, T, Saito, M 1998a. Anti-obesity effects of selective agonists to the β3-adrenergic receptor in dogs. I. The presence of Canine β3-adrenergic receptor and in vivo lipomolibization by its agonists. The Journal of Veterinary Medical Science 60, 459–463.Google Scholar
Sasaki, N, Uchida, E, Niiyama, M, Yoshida, T, Saito, M 1998b. Anti-obesity effects of selective agonists to the β3-adrenergic receptor in dogs. II. Recruitment of thermogenic brown adipocytes and reduction of adiposity after chronic treatment with a β3-adrenergic agonist. The Journal of Veterinary Medical Science 60, 465–469.Google Scholar
Sheridan, R, Ferreira, AV, Hoffman, LC 2003. Production efficiency of South African Mutton Merino lambs and Boer goat kids receiving either a low or a high energy feedlot diet. Small Ruminant Research 50, 75–82.Google Scholar
Skeberdis, VA, Gendviliene, V, Zablockaite, D, Treinys, R, Macianskiene, R, Bogdelis, A, Jurevicius, J, Fischmeister, R 2008. β3-adrenergic receptor activation increases human atrial tissue contractility and stimulates the L-type Ca2+ current. The Journal of Clinical Investigation 118, 3219–3227.Google Scholar
Strosberg, AD 1997. Structure and function of the beta 3-adrenergic receptor. Annual Review of Pharmacology and Toxicology 37, 421–450.Google Scholar
Sumner, JM, McNamara, JP 2007. Expression of lipolytic genes in the adipose tissues of pregnant and lactating holstein cattle. Journal of Dairy Science 90, 5237–5246.Google Scholar
Trebicka, J, Hennenberg, M, Schulze Pröbsting, A, Laleman, W, Klein, S, Granzow, M, Nevens, F, Zaagsma, J, Heller, J, Sauerbruch, T 2009. Role of beta(3)-adrenoceptors for intrahepatic resistance and portal hypertension in liver cirrhosis. Hepatology 50, 1–12.Google Scholar
Van Baak, MA, Hul, GB, Toubro, S, Astrup, A, Gottesdiener, KM, DeSmet, M, Saris, WH 2002. Acute effect of L-796568, a novel beta 3-adrenergic receptor agonist, on energy expenditure in obese men. Clinical Pharmacology and Therapeutics 71, 272–279.Google Scholar
Weyer, C, Gautier, JF, JrDanforth, E 1999. Development of beta 3-adrenoceptor agonists for the treatment of obesity and diabetes-an update. Diabetes and Metabolism 25, 11–21.Google Scholar