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Dietary supplementation of weaned piglets with a yeast-derived mannan-rich fraction modulates cecal microbial profiles, jejunal morphology and gene expression

Published online by Cambridge University Press:  07 January 2019

J. M. Fouhse ,
K. Dawson ,
D. Graugnard ,
M. Dyck  and
B. P. Willing
J. M. Fouhse
Affiliation:
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6E 2P5
K. Dawson
Affiliation:
Center for Animal Nutrigenomics and Applied Animal Nutrition, Alltech Inc., Nicholasville, KY 40356, USA
D. Graugnard
Affiliation:
Center for Animal Nutrigenomics and Applied Animal Nutrition, Alltech Inc., Nicholasville, KY 40356, USA
M. Dyck
Affiliation:
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6E 2P5
B. P. Willing*
Affiliation:
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6E 2P5
*
†E-mail: [email protected]
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Abstract

The development of nutritional strategies to improve microbial homeostasis and gut health of piglets post-weaning is required to mitigate the high prevalence of post-weaning diarrhea and subsequent growth checks typically observed during the weaning transition. Therefore the objective of this study was to determine the effect of supplementing piglet creep and nursery feed with a yeast-derived mannan-rich fraction (MRF) on piglet growth performance, cecal microbial profiles, and jejunal morphology and gene expression. Ten litters of piglets (n=106) were selected on postnatal day (PND) 7 and assigned to diets with or without MRF (800 mg/kg) until weaning (n=5 litters/treatment; initial weight 3.0±0.1 kg). On PND 21, 4 piglets per litter (n=40) were selected and weaned into the nursery where they remained on their respective diets until PND 42. A two-phase feeding program was used to meet nutrient requirements, and pigs were switched from phase 1 to phase 2 on PND 28. Feed intake and piglet weights were recorded on PND 7, 14, 21, 28, 35 and 42. On PND 28 and 42, ten piglets per treatment were euthanized to collect intestinal tissue and digesta. Piglets supplemented with MRF had 21.5% greater (P<0.05) average daily feed intake between PND 14-21. However, MRF supplementation did not affect piglet growth performance compared to control. On PND 28, jejunal villus height was 16.8% greater (P<0.05) in piglets consuming MRF supplemented diets. Overall microbial community structure in cecal digesta on PND 28 tended to differ in pigs supplemented with MRF (P=0.076; analysis of similarities (ANOSIM)) with increased (P<0.05) relative abundance of Paraprevotellaceae genera YRC22 and CF231, and reduced (P<0.05) relative abundance of Sutterella and Prevotella. Campylobacter also tended to reduce (P<0.10) in MRF supplemented piglets. On PND 28 differential gene expression in jejunal tissue signified an overall effect of supplementing MRF to piglets. Downstream analysis of gene expression data revealed piglets supplemented with MRF had enriched biological pathways involved in intestinal development, function and immunity, supporting the observed improvement in jejunal villus architecture on PND 28. On PND 42 there was no effect of MRF supplementation on jejunal morphology or overall cecal microbial community structure. In conclusion, supplementing Actigen™, a MRF, to piglets altered cecal microbial community structure and improved jejunal morphology early post-weaning on PND 28, which is supported by enrichment of intestinal development pathways.

Keywords

yeast cell wallpigmicrobiotamicroarrayintestine
Type
Research Article
Information
animal , Volume 13 , Issue 8 , August 2019 , pp. 1591 - 1598
Copyright
© The Animal Consortium 2019 

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References

Andrés-Barranco, S, Vico, JP, Grilló, MJ and Mainar-Jaime, RC 2015. Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide. Journal of Applied Microbiology 118, 284–294.Google Scholar
Barreto-Bergter, E and Figueiredo, RT 2014. Fungal glycans and the innate immune recognition. Frontiers in Cellular and Infection Microbiology 4, 1–17.Google Scholar
Burrough, E, Terhorst, S, Sahin, O and Zhang, Q 2013. Prevalence of Campylobacter spp. relative to other enteric pathogens in grow-finish pigs with diarrhea. Anaerobe 22, 111–114.Google Scholar
Canadian Council on Animal Care (CCAC) 2009. Guidelines on: the care and use of farm animals in research, teaching and testing. CCAC, Ottawa, ON, Canada.Google Scholar
Caporaso, JG, Kuczynski, J, Stombaugh, J, Bittinger, K, Bushman, FD and Bostello, EK 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7, 335–336.Google Scholar
Caruso, R, Marafini, I, Franze, E, Stolfi, C, Zorzi, F, Monteleone, I, Caprioli, F, Colantoni, A, Sarra, M, Sedda, S, Biancone, L, Sileri, P, Sica, GS, MacDonald, TT, Pallone, F and Monteleone, G 2014. Defective expression of SIRT1 contributes to sustain inflammatory pathways in the gut. Mucosal Immunology 7, 1467–1479.Google Scholar
Castillo, M, Martín-Orúe, SM, Taylor-Pickard, JA, Pérez, JF and Gasa, J 2008. Use of mannan-oligosaccharides and zinc chelate as growth promoters and diarrhea preventative in weaning pigs: effects on microbiota and gut function. Journal of Animal Science 86, 94–101.Google Scholar
Che, TM, Johnson, RW, Kelley, KW, Dawson, KA, Moran, CA and Pettigrew, JE 2012. Effects of mannan oligosaccharide on cytokine secretions by porcine alveolar macrophages and serum cytokine concentrations in nursery pigs. Journal of Animal Science 90, 657–668.Google Scholar
Chen, L, Xu, Y, Chen, X, Fang, C, Zhao, L and Chen, F 2017. The maturing development of gut microbiota in commercial piglets during the weaning transition. Frontiers in Microbiology 8, 1–13.Google Scholar
Corrigan, A, Corcionivoschi, N and Murphy, RA 2017. Effect of yeast mannan-rich fractions on reducing Campylobacter colonization in broiler chickens. The Journal of Applied Poultry Research 26, 350–357.Google Scholar
Edgar, RC 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods 10, 996–998.Google Scholar
Edgar, RC, Haas, BJ, Clemente, JC, Quince, C and Knight, R 2011. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics 27, 2194–2200.Google Scholar
Edwards, MV, Edwards, AC, Millard, P and Kocher, A 2014. Mannose rich fraction of Saccharomyces cerevisiae promotes growth and enhances carcass yield in commercially housed grower–finisher pigs. Animal Feed Science and Technology 197, 227–232.Google Scholar
Graugnard, DE, Samuel, RS, Xiao, R, Spagnler, LF and Brennan, KM 2015. Intestinal gene expression profiles of piglets benefit from maternal supplementation with a yeast mannan-rich fraction during gestation and lactation. Animal 9, 622–628.Google Scholar
Halas, V and Nochta, I 2012. Mannan oligosaccharides in nursery pig nutrition and their potential mode of action. Animals 2, 261–274.Google Scholar
Harvey, RB, Young, CR, Ziprin, RL, Hume, ME, Genovese, KJ, Anderson, RC, Drolesky, RE, Stanker, LH and Nisbet, DJ 1999. Prevalence of Campylobacter spp isolated from the intestinal tract of pigs raised in an integrated swine production system. Journal of the American Veterinary Medical Association 215, 1601–1604.Google Scholar
Hooge, DM, Kiers, A and Connolly, A. 2013. Meta-analysis summary of broiler chicken trials with dietary MRF (2009-2012). International Journal of Poultry Science 12, 1–8.Google Scholar
Hopwood, D and Hampson, D 2003. Interactions between the intestinal microflora, diet and diarrhoea, and their influences on piglet health in the immediate post-weaning period. In Weaning the pig: concepts and consequences (ed. Pluske, JR, Le Dividich, J and Verstegen, MWA), pp. 199–217. Wageningen Academic Publisher, Wageningen, The Netherlands.Google Scholar
Horio, Y, Hayashi, T, Kuno, A and Kunimoto, R 2011. Cellular and molecular effects of sirtuins in health and disease. Clinical Science 121, 191–203.Google Scholar
Jiang, Z, Wei, S, Wang, Z, Zhu, C, Hu, S, Zheng, C, Chen, Z, Hu, Y, Wang, L, Ma, X and Yang, X 2015. Effects of different forms of yeast Saccharomyces cerevisiae on growth performance, intestinal development, and systemic immunity in early-weaned piglets. Journal of Animal Science and Biotechnology 6, 47.Google Scholar
Kaemmerer, E, Kuhn, P, Schneider, U, Clahsen, T, Jeon, MK, Klaus, C, Andruszkow, J, Härer, M, Ernst, S, Schipers, A, Wagner, N and Gassler, N 2015. Beta-7 integrin controls enterocyte migration in the small intestine. World Journal of Gastroenterology 21, 1759–1764.Google Scholar
Lallès, JP, Boudry, G, Favier, C, Le Floc’h, N, Luron, I, Montagne, L, Oswald, L, Pié, S, Piel, C and Sève, B 2004. Gut function and dysfunction in young pigs: physiology. Animal Research 53, 301–316.Google Scholar
Louis, P and Flint, HJ 2009. Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine. FEMS Microbiology Letters 294, 1–8.Google Scholar
Masella, AP, Bartram, AK, Truszkowski, JM, Brown, DG and Neufeld, JD 2012. PANDAseq: paired-end assembler for illumina sequences. BMC Bioinformatics 13, 31.Google Scholar
Miguel, JC, Rodriguez-Zas, SL and Pettigrew, JE 2004. Efficacy of a mannan oligosaccharide (Bio-Mos®) for improving nursery pig performance. Journal of Swine Health and Production 12, 296–307.Google Scholar
National Research Council (NRC) 2012. Nutrient requirements of swine, 11th revised edition. National Academies Press, Washington, DC, USA.Google Scholar
Navas-Molina, JA, Peralta-Sánchez, JM, González, A, McMurdie, PJ, Vázquez-Baeza, Y, Xu, Z, Ursell, LK, Lauber, C, Zhou, H, Song, SJ, Huntly, J, Ackermann, GL, Berg-Lyons, D, Holmes, S, Caporaso, JG and Knight, R 2013. Advancing our understanding of the human microbiome using QIIME. Methods in Enzymology 531, 371–444.Google Scholar
Patterson, AM and Watson, AJM 2017. Deciphering the complex signaling systems that regulate intestinal epithelial cell death processes and shedding. Frontiers in Immunology 8, 841.Google Scholar
Peng, L, Li, ZR, Green, RS, Holzman, IR and Lin, J 2009. Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP-activated protein kinase in Caco-2 cell monolayers. Journal of Nutrition 139, 1619–1625.Google Scholar
Pickert, G, Neufert, C, Leppkes, M, Zheng, Y, Wittkopf, N, Warntjen, M, Lehr, HA, Hirth, S, Weigmann, B, Wirtz, S, Ouyang, W, Neurath, MF and Becker, C 2009. STAT3 links IL-22 signaling in intestinal epithelial cells to mucosal wound healing. The Journal of Experimental Medicine 206, 1465–1472.Google Scholar
Pluske, JR, Thompson, MJ, Atwood, CS, Bird, PH, Williams, IH and Hartmann, PE 1996. Maintenance of villus height and crypt depth, and enhancement of disaccharide digestion and monosaccharide absorption, in piglets fed on cows’ whole milk after weaning. British Journal of Nutrition 76, 409.Google Scholar
Sun, X and Zhu, MJ 2017. AMP-activated protein kinase: a therapeutic target in intestinal diseases. Open Biology 7, 170104.Google Scholar
Swanson, KS, Grieshop, CM, Flickinger, EA, Bauer, LL, Healy, HP, Dawson, KA, Merchen, NR and Fahey, GC 2002. Supplemental fructooligosaccharides and mannanoligosaccharides influence immune function, ileal and total tract nutrient digestibilities, microbial populations and concentrations of protein catabolites in the large bowel of dogs. The Journal of Nutrition 132, 980–989.Google Scholar
Wang, Q, Garrity, GM, Tiedje, JM and Cole, JR 2007. Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology 73, 5261–5267.Google Scholar
Wang, W, Li, Z, Han, Q, Guo, Y, Zhang, B and D’Ica, R 2016. Dietary live yeast and mannan-oligosaccharide supplementation attenuate intestinal inflammation and barrier dysfunction induced by Escherichia coli in broilers. British Journal of Nutrition 116, 1878–1888.Google Scholar
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