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Dietary fat source affects metabolism of fatty acids in pigs as evaluated by altered expression of lipogenic genes in liver and adipose tissues

Published online by Cambridge University Press:  01 April 2009

P. Duran-Montgé ,
P. K. Theil ,
C. Lauridsen  and
E. Esteve-Garcia
P. Duran-Montgé
Affiliation:
CENTA, IRTA Building A – Finca Camps i Armet E-17121 Monells (Girona), Spain
P. K. Theil
Affiliation:
Department of Animal Health, Welfare, and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Research Centre Foulum, Tjele, Denmark
C. Lauridsen
Affiliation:
Department of Animal Health, Welfare, and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, Research Centre Foulum, Tjele, Denmark
E. Esteve-Garcia*
Affiliation:
IRTA, Mas de Bover, Ctra. de Reus-El Morell km. 3, 8 E-43120 Constantí (Tarragona), Spain
*
E-mail: [email protected]
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Abstract

Little is known about pig gene expressions related to dietary fatty acids (FAs) and most work have been conducted in rodents. The aim of this study was to investigate how dietary fats regulate fat metabolism of pigs in different tissues. Fifty-six crossbred gilts (62 ± 5.2 kg BW) were fed one of seven dietary treatments (eight animals per treatment): a semi-synthetic diet containing a very low level of fat (no fat (NF)) and six fat-supplemented diets (ca. 10%) based on barley and soybean meal. The supplemental fat sources were tallow (T), high-oleic sunflower oil (HOSF), sunflower oil (SFO), linseed oil (LO), blend (FB) (55% T, 35% SFO and 10% LO) and fish oil (FO) blend (40% FO and 60% LO). Pigs were slaughtered at 100 kg BW and autopsies from liver, adipose tissue and muscle semimembranousus were collected for qPCR. The messenger ribonucleic acid (mRNA) abundances of genes related to lipogenesis were modified due to dietary treatments in both liver (sterol regulatory element-binding protein-1 (SREBP-1), acetyl CoA carboxylase (ACACA) and stearoyl CoA desaturase (SCD)) and adipose tissue (fatty acid synthase (FASN), ACACA and SCD), but were not affected in semimembranousus muscle. In the liver, the mRNA abundances of genes encoding lipogenic enzymes were highest in pigs fed HOSF and lowest in pigs fed FO. In adipose tissue, the mRNA abundances were highest in pigs fed the NF diet and lowest in pigs fed T. The study demonstrated that dietary FAs stimulate lipogenic enzyme gene expression differently in liver, fat and muscles tissues.

Keywords

swinelipidstranscriptomicslipogenesis
Type
Full Paper
Information
animal , Volume 3 , Issue 4 , April 2009 , pp. 535 - 542
Copyright
Copyright © The Animal Consortium 2008

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