Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-26T14:19:18.923Z Has data issue: false hasContentIssue false
  • English
  • Français

The detoxication of nitrate by two antioxidants or a probiotic, and the effects on blood and seminal plasma profiles and reproductive function of New Zealand White rabbit bucks

Published online by Cambridge University Press:  26 November 2012

Y. A. Attia ,
E. A. Abd El Hamid ,
A. M. Ismaiel  and
A. El-Nagar
Y. A. Attia*
Affiliation:
Arid Land Agriculture Department, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, P.O. Box 80208, Jeddah 21589, Saudi Arabia Department of Animal and Poultry Production, Faculty of Agriculture, Damanhour University, Damanhour 22516, Egypt
E. A. Abd El Hamid
Affiliation:
Department of Animal and Poultry Production, Faculty of Agriculture, Damanhour University, Damanhour 22516, Egypt
A. M. Ismaiel
Affiliation:
Department of Animal and Poultry Production, Faculty of Agriculture, Damanhour University, Damanhour 22516, Egypt
A. El-Nagar
Affiliation:
Department of Animal and Poultry Production, Faculty of Agriculture, Damanhour University, Damanhour 22516, Egypt
*
†E-mail: [email protected]
Get access

Abstract

Forty-two New Zealand White male rabbits were housed individually in wire cages and randomly distributed among six experimental groups of seven rabbits each, during 16 to 61 weeks of age. There were three main nitrate groups: 0 (tap water), 350 and 700 ppm. Within the 700 ppm of nitrate, there were four subgroups, in which one group was used as control group and the other three groups were supplemented with either 200 ppm of ascorbic acid (vitamin (Vit) C), 200 ppm of Vit E with 0.2 ppm of selenium (Se) and 1000 ppm of probiotic. The nitrate was supplemented as a sodium nitrate. The aim is to test the ability of Vit C and Vit E, Se and probiotic on the deleterious effects (blood and seminal plasma biochemical constituents, semen quality and productive performance) of nitrate in drinking water. Rabbits given nitrate at 700 ppm had significantly lower plasma globulin, red blood cells (RBCs), hemoglobin (Hgb), packed cell volume % (PCV%) and total antioxidant capacity (TAC) than those given the other concentrations of nitrate. Vit C, Vit E with Se and probiotic resulted in significantly (P < 0.05) greater Hgb, RBCs, PCV% and TAC than those of bucks given water supplemented with only 700 ppm nitrate, but the aspartate aminotransferase and alanine aminotransferase concentrations in seminal plasma were lower. Testosterone in the blood plasma and the seminal plasma was significantly (P < 0.05) lower in rabbits given 700 ppm nitrate than in those given other concentrations of nitrate. Vit C, Vit E with Se and the probiotic significantly increased testosterone, fertility, number of offspring and total offspring weight of rabbits sired by bucks supplemented with 700 ppm of nitrate.

Keywords

rabbitsnitratesemen qualityreproductive performance
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 4 , April 2013 , pp. 591 - 601
Copyright
Copyright © The Animal Consortium 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abd EL-Hamid, EA 2006. Effect of water supplies on some blood hematological, biochemical, histopathological parameters and growth performance of female Japanese quails. Alexandria University Journal of Agriculture and Environmental Sciences 5, 117–139.Google Scholar
Alabdula'aly, AI, Al-Rehaili, AM, Al-Zarah, AI, Khan, MA 2010. Assessment of nitrate concentration in groundwater in Saudi Arabia. Environmental Monitoring Assessment 161, 1–9.Google Scholar
Association of Official Analytical Chemists (AOAC) 2007. Official Methods of Analysis, 19th edition. AOAC, Arlington, VA, USA.Google Scholar
Atef, M, Abo-Norage, MA, Hanafy, MS, Agag, AE 1991. Pharmacotoxicological aspects of nitrate and nitrite in domestic fowls. British Poultry Science 32, 399–404.Google Scholar
Attia, YA, Kamel, KI 2012. Semen quality, testosterone level, seminal plasma biochemical profile and antioxidant statues of V-line rabbit bucks supplemented with different levels of soybean lecithin. Animal 6, 824–833.Google Scholar
Attia, YA, Hassan, RA, Qota, MA 2009. Recovery from adverse effects of heat stress on slow-growing chicks in the tropics 1: effect of ascorbic acid and different levels of betaine. Tropical Animal health and Production 41, 807–818.Google Scholar
Attia, YA, Al-Hanoun, A, Bovera, F 2011. Effect of different levels of bee pollen on performance and blood profile of New Zealand White bucks and growth performance of their offspring during summer and winter months. Journal of Animal Physiology and Animal Nutrition 95, 17–26.Google Scholar
Attia, YA, Hassan, RA, Tag El-Din, AE, Abou-Shehema, BM 2011. Effect of ascorbic acid or increasing metabolizable energy level with or without supplementation of some essential amino acids on productive and physiological traits of slow-growing chicks exposed to chronic heat stress. Journal of Animal Physiology and Animal Nutrition 95, 744–755.CrossRefGoogle ScholarPubMed
Attia, YA, Abdalah, AA, Zeweil, HS, Bovera, F, Tag El-Din, AA, Araft, MA 2010. Effect of inorganic or organic selenium supplementation on productive performance, egg quality and some physiological traits of dual purpose breeding hens. Cezh Journal of Animal Science 55, 505–519.Google Scholar
Bassuny, SM, Shehata, SA, Bahgat, LB, Mohamed, SIA 2004. Nitrate toxicity in rabbits: 1 – effect of nitrate in drinking water on digestion, some blood constituents and growth performance of growing rabbits. Egyptian Journal of Rabbit Science 14, 147–158.Google Scholar
Blom, E 1950. A one min live–dead sperm by means of eosin–nigrosin. Fertility and Sterility 1, 176–177.Google Scholar
Botsoglou, N, Florou-Paneri, P, Christaki, E, Giannenas, I, Spais, A 2004. Performance of rabbits and oxidative stability of muscle tissues as affected by dietary supplementation with oregano essential oil. Archive Animal Nutrition 58, 209–218.Google Scholar
Bratton, R, Foote, W, Shipman, K 1956. Procedure for counting bovine sperm with a haemocytometer and calibration used of photometers to estimate sperm count by optical operation density. Animal Breeding Laboratory Procedure No. 40. Cornell University, Ithaca, NY, USA.Google Scholar
Brun, JM, Theau-Clément, M, Bolet, G 2002. The relationship between rabbit semen characteristics and reproductive performance after artificial insemination. Animal Reproduction Science 70, 139–149.Google Scholar
Burow, KR, Nolan, BT, Rupert, MG, Dubrovsky, NM 2010. Nitrate in groundwater of the United States, 1991–2003. Environmental Science & Technology 44, 4988–4997.Google Scholar
Castellini, C, Lattaioli, P, Barnardini, M, Dal Bosco, A 2000. Effect of dietary α-tocopheryl acetate and ascorbic acid on rabbit semen stored at 5C. Theriogenology 54, 523–533.Google Scholar
Castellini, C, Lattaioli, P, Dal Bosco, A, Beghelli, D 2002. Effect of supranutritional level of dietary α-tocopherol acetate and selenium on rabbit. Theriogenology 58, 1723–1732.Google Scholar
Castellini, C, Lattaioli, P, Dal Bosco, A, Minelli, A, Mugnai, C 2003. Oxidative status and semen characteristics of rabbit buck as affected by dietary vitamin E, C and n-3 fatty acids. Reproductive Nutrition Development 43, 91–103.Google Scholar
Chaudhary, V, Kumar, M, Sharma, M, Yadav, BS 2010. Fluoride, boron and nitrate toxicity in ground water of northwest Rajasthan, India. Environmental Monitoring Assessment 161, 343–348.Google Scholar
Chow, CK, Hong, CB 2002. Dietary vitamin E and selenium and toxicity of nitrite and nitrate. Toxicology 180, 195–207.Google Scholar
Cockburn, A, Brambilla, G, Fernández, M-L, Arcella, D, Bordajandie, LR, Cottrill, B, van Peteghem, C, Dorne, J-L 2010. Nitrite in feed: from animal health to human health. Toxicology and Applied Pharmacology, doi:10.1016/j.taap.2010.11.008.Google Scholar
Coles, EH 1974. Veterinary clinical pathology, 2nd edition. W.B. sanders Co., Philadelphia, London, Toronto.Google Scholar
Culling, CF 1983. Handbook of histopathological and histochemical techniques, 3rd edition. Butterworth, London, UK.Google Scholar
Djekoun-Bensoltane, S, Kammerer, M, Larhantec, M, Pilet, N, Thorin, C 2007. Nitrate and nitrite concentrations in rabbit saliva: comparison with rat saliva. Environmental Toxicology Pharmacology 23, 132–134.Google Scholar
Doumas, BT, Bayso, DD, Carter, RJ, Peters, T, Schaffer, R 1981. Determination of total serum protein. Clinical Chemistry 27, 1642–1643.Google Scholar
Edwards, TM, Guillette, LJ 2007. Reproductive characteristics of male mosquitofish (Gambusia holbrooki) from nitrate-contaminated springs in Florida. Aquatic Toxicology 85, 40–47.Google Scholar
EL-Kelawy, HM 2003. Effect of supplemental levels of vitamin E and selenium on growth performance, blood serum constituents, organs-histopathology and reproductive efficiency of Bouscat rabbits. Egyptian Journal of Rabbit Science 13, 117–134.Google Scholar
Foresti, R, Clark, JE, Green, CJ, Motterlini, R 1997. Thiol compounds interact with nitric oxide in regulating heme oxygenase-1 induction in endothelial cells. Involvement of superoxide and peroxynitrite anions. Journal of Biology Chemistry 272, 18411–18417.Google Scholar
Fried, JJ 1991. Nitrates and their control in the EEC aquatic environment. In Nitrate contamination: Exposure, consequence and control, NATO ASI Serial G: Ecological Sciences 309 (ed. I Bogardi and RD Kuzelka), pp. 3–11. Springer-Verlag, Berlin.Google Scholar
Gilman, AP, Villeneuve, DC, Secours, VE, Yagminas, AP, Tracy, BL, Quinn, JM, Valli, VE, Moss, MA 1998. Uranyl nitrate: 91-day toxicity studies in the New Zealand white rabbit. Toxicology Science 41, 129–137.Google Scholar
Gow, AJ, Thom, SR, Chiropoulos, HIS 1998. Nitrate oxide and peroxynitrite-mediated pulmonary cell death. American Journal of Physiology 274, 112–118.Google Scholar
Guzik, TJ, West, NEJ, Black, E, Mcdonald, D, Ratnatunga, R, Channon, KM 2000. Vascular superoxide production by DNA (p) H oxidase: association with endothelial dysfunction and clinical risk factors. Circulation Research 86, e85–e90.Google Scholar
Hairston, JE 1995. Drinking water for livestock and poultry. Alabama A&M and Auburn Universities. Retrieved March 20, 2005, from http://www.Aoes.edu.Google Scholar
Hawkey, CM, Dennett, TB 1989. A color atlas of comparative veterinary hematology. Wolf publishing Limited, London, England.Google Scholar
Helper, OE 1966. Manual of clinical laboratory methods. Thomas Spring Field, Illinois, USA.Google Scholar
Hirneth, H, Classen, HG 1984. Inhibition of nitrate-induced increase of plasma nitrite and methemoglobinemia in rats by simultaneous feeding of ascorbic acid or tocopherol. Arzneimittelforschung 34, 988–991.Google Scholar
Hord, NG, Tang, Y, Bryan, NS 2009. Food sources of nitrates and nitrites: the physiologic context for potential health benefits. American Journal Clinical Nutrition 90, 1–10.Google Scholar
Kammerer, M, Siliart, B 1993. Midterm toxicity of nitrates: experimental evaluation of the effect on reproductive functions in the female rabbits. Veterinary Research 24, 43–44.Google Scholar
Kannan, D, Viswanathan, K, Mohan, B 2007. The effect of effect of feeding virginiamycin and LactoBacillus sporogenes on broiler production performance characters. Tamilnadu Journal of Veterinary and Animal Sciences 3, 106–108.Google Scholar
Kawahara, E, Ueda, DT, Nomura, S 1991. In vitro phagocytic activity of white spotted shark cells after injection with Aeromonas salmonicida extracellular products, vol. 26. Gyobyo Kenkyu, Japan, 213–214.Google Scholar
Lucky, Z 1977. Methods for the diagnosis of fish diseases. Ameruno Publishing Co, PVT, Ltd, New Delhi, Bomby, New York.Google Scholar
Mahboob, S, Sherif, AN, Shakoori, AR, Raza, SH, Andleeb, S 2001. Effect of nitrate and nitrite pollution on some haematological parameters of rabbits. Pakistan Journal of Agriculture Science 38, 44–46.Google Scholar
Manassaram, DM, Backer, LC, Moll, DM 2006. A review of nitrates in drinking water: maternal exposure and adverse reproductive and developmental outcomes. Environmental Health Perspectives 114, 320–327.Google Scholar
Martarelli, D, Verdenelli, MC, Scuri, S, Cocchioni, M, Silvi, S, Cecchini, C, Pompei, P 2011. Effect of a probiotic intake on oxidant and antioxidant parameters in plasma of athletes during intense exercise training. Current Microbiology 62, 1689–1696.Google Scholar
Masukawa, T, Iwata, H 1979. Protective effect of selenite on nitrite toxicity. Experientia 35, 1360–1361.Google Scholar
Montville, TJ, Mattews, KR 2005. Food microbiology: an introduction. ASM Press, Washington, DC.Google Scholar
Morrissey, P, Buckley, D, Sheehy, P, Monahan, F 1994. Vitamin E and meat quality. Proceeding Nutrition Society 53, 289–295.Google Scholar
National Research Council (NRC) 1974. Nutrient and toxic substances in water for livestock and poultry. Washington, DC, USA.Google Scholar
Nielsen, VG, Crow, JP 2004. Peroxynitrite decreased rabbit tissue factor activity in vitro. Anesthesia Analgesia 98, 668–671.Google Scholar
Obeidat, MM, Massadeh, AM, Al-Ajlouni, AM, Athamneh, FS 2007. Analysis and evaluation of nitrate levels in groundwater at Al-Hashimiya area, Jordan. Environmental Monitoring Assessments 135, 475–486.CrossRefGoogle ScholarPubMed
Okafor, PN, Ogbonna, UI 2003. Nitrate and nitrite contamination of water sources and fruit juices marketed in South-Eastern Nigeria. Journal of Food Composition and Analysis 16, 213–218.Google Scholar
Rama Rao, SV, Nagalakshmi, S, Reddy, VR 2002. Feeding to minimize heat stress. Poultry International 41, 30–33.Google Scholar
Reed, D 1992. Interaction of vitamin E, ascorbic acid, and glutathione in protection against oxidative damage. In Vitamin E in health and disease (ed. L Packer and J Fuchs), pp. 269–281. Marcel Dekker, New York.Google Scholar
Reinhold, RR 1953. Determination of serum albumin. Clinical Chemistry 21, 1370–1372.Google Scholar
Reitman, MA, Frankel, AF 1957. Determination of liver enzymes. Clinical Chemistry 21, 1234–1237.Google Scholar
Robson, S 2007. Nitrate and nitrite poisoning in livestock. Primefact 415, nitrate and nitrite poisoning in livestock. Retrieved January 30, 2008, from http://www.dpi.nsw.gov.au/reader/feeding-residues/6501.Google Scholar
Rolfe, RD 2000. The role of probiotic cultures in the control of gastrointestinal health. Journal of Nutrition 130, 396S–402S.Google Scholar
Roth, E 2000. Oxygen free radicals and their clinical implications. Acta Chirurgica Humgarica 36, 302–305.Google Scholar
Saleh, ZA, Brunn, H, Paetzold, R, Hussein, L 1998. Nutrients and chemical residues in an Egyptian total mixed diet. Food Chemistry 63, 535–541.Google Scholar
Shehata, SA 2005. Nitrate detoxification of drinking water by ascorbic acid in growing rabbits. World Rabbit Science 13, 93–106.Google Scholar
Statistical Analysis Systems Institute (SAS) 2002. In SAS/STAT user's guide. SAS Institute Inc, Cary, NC, USA.Google Scholar
Thomson, BM, Nokes, CJ, Cressey, PJ 2007. Intake and risk assessment of nitrate and nitrite from New Zealand foods and drinking water. Food Additive Contamination 24, 113–121.Google Scholar
Tietz, NW 1982. Fundamental of clinical chemistry. Norbert Sounder Company, Philadelphia, USA.Google Scholar
Umar, IA, Toma, I, Akombum, CA, Nnadi, CJ, Mahdi, MA, Gidado, A, Igbokwe, IO, Buratai, LB 2010. The role of intraperitoneally administered vitamin C during Trypanosoma congolense infection of rabbits. African Journal of Biotechnology 9, 5224–5228.Google Scholar
Van Soest, PJ, Wine, RM 1967. Use of detergent in the analysis of fibrous feed. IV. Determination of plant cell wall constituent. Journal Association of Analytical Chemistry 50, 50–55.Google Scholar
Vermeer, IT, Monnen, EJ, Dallingam, JW, Kleijans, JC, Van Maane, JM 1999. Effect of ascorbic acid and green tea on endogenous formation of N-nitrosodimethylamine and N-nitrosopiperidine in human. Mutation Research 428, 353–361.Google Scholar
Wahaab, RA, Badawy, MI 2004. Water quality assessment of the river Nile system: an overview. Biomed Environmental Science 17, 87–100.Google Scholar
Ward, MH, Kilfoy, BA, Weyer, PJ, Anderson, KE, Folson, AR, Cerhan, JR 2010. Nitrate intake and the risk of thyroid cancer and thyroid disease. Epidemiology 21, 389–395.Google Scholar
Watson, D 1960. A simple method for determination of serum cholesterol. Clinical Chemistry Acta 5, 637–643.Google Scholar
Wintrobe, MM 1965. Clinical hematology, 5th edition. Lea and Febiger Philadelphia, USA.Google Scholar
Yasmina, M, Abdennour, C 2008. Influence of vitamin C on testicular functions of domestic rabbit oryctolagus cuniculus under mercury exposure. European Journal of Scientific Research 22, 197–204.Google Scholar
Zhang, WL, Tian, ZX, Li, XQ 1996. Nitrate pollution of groundwater in Northern China. Agriculture, Ecosystems and Environment 59, 223–231.Google Scholar