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Coronary angiography and percutaneous coronary intervention in the porcine model: a practical guide to the procedure

Published online by Cambridge University Press:  20 September 2011

P. D. Williams ,
N. Malik  and
P. A. Kingston
P. D. Williams*
Affiliation:
The University of Manchester, Manchester Academic Health Science Centre, School of Biomedicine, Vascular Gene Therapy Unit, Core Technology Facility, 46 Grafton Street, Manchester M13 9NT, UK
N. Malik
Affiliation:
The University of Manchester, Manchester Academic Health Science Centre, School of Biomedicine, Vascular Gene Therapy Unit, Core Technology Facility, 46 Grafton Street, Manchester M13 9NT, UK
P. A. Kingston
Affiliation:
The University of Manchester, Manchester Academic Health Science Centre, School of Biomedicine, Vascular Gene Therapy Unit, Core Technology Facility, 46 Grafton Street, Manchester M13 9NT, UK
*
†E-mail: [email protected]
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Abstract

Assessment of safety and efficacy within the porcine coronary artery model remains a standard requirement for new therapies delivered to the coronary arteries before proceeding to clinical testing. Human coronary procedures carry a very low mortality rate; however, procedural mortality for porcine experiments is often high, despite these animals being young and free of atherosclerosis. Some of these deaths are due to poor technique, and therefore avoidable. However, despite the wide use of this model, a systematic description of the procedure has never been published. This article will detail how porcine angiography and stent implantation is performed in our institution and will discuss the relevant differences between humans and pigs with regard to anaesthesia, pharmacotherapy, vascular access, catheter selection and angiographic views. Important variations to the technique that have been reported are also covered.

Keywords

pigcoronary arterymethod
Type
Full Paper
Information
animal , Volume 6 , Issue 2 , 03 January 2012 , pp. 311 - 320
Copyright
Copyright © The Animal Consortium 2011

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References

Appleby, CE, Kingston, PA, David, A, Gerdes, CA, Umana, P, Castro, MG, Lowenstein, PR, Heagerty, AM 2003. A novel combination of promoter and enhancers increases transgene expression in vascular smooth muscle cells in vitro and coronary arteries in vivo after adenovirus-mediated gene transfer. Gene Therapy 10, 1616–1622.CrossRefGoogle ScholarPubMed
Ashwini, C, Shuba, R, Jayanthi, K 2008. Comparative anatomy of the circle of Willis in man, cow, sheep, goat, and pig. Neuroanatomy 7, 54–65.Google Scholar
Chung, IM, Kim, J, Pak, YK, Jang, Y, Yang, WI, Han, I, Park, SJ, Park, SW, Huh, J, Wight, TN, Ueno, H 2010. Blockade of TGF-beta by catheter-based local intravascular gene delivery does not alter the in-stent neointimal response, but enhances inflammation in pig coronary arteries. International Journal of Cardiology 145, 468–475.CrossRefGoogle Scholar
Crick, SJ, Sheppard, MN, Ho, SY, Gebstein, L, Anderson, RH 1998. Anatomy of the pig heart: comparisons with normal human cardiac structure. Journal of Anatomy 193 (Pt 1), 105–119.CrossRefGoogle ScholarPubMed
Edelman, ER, Seifert, P, Groothuis, A, Morss, A, Bornstein, D, Rogers, C 2001. Gold-coated NIR stents in porcine coronary arteries. Circulation 103, 429–434.CrossRefGoogle ScholarPubMed
Frimerman, A, Welch, PJ, Jin, X, Eigler, N, Yei, S, Forrester, J, Honda, H, Makkar, R, Barber, J, Litvack, F 1999. Chimeric DNA–RNA hammerhead ribozyme to proliferating cell nuclear antigen reduces stent-induced stenosis in a porcine coronary model. Circulation 99, 697–703.CrossRefGoogle Scholar
Granada, JF, Kaluza, GL, Wilensky, RL, Biedermann, BC, Schwartz, RS, Falk, E 2009. Porcine models of coronary atherosclerosis and vulnerable plaque for imaging and interventional research. EuroIntervention 5, 140–148.CrossRefGoogle ScholarPubMed
Gunn, J, Arnold, N, Chan, KH, Shepherd, L, Cumberland, DC, Crossman, DC 2002. Coronary artery stretch versus deep injury in the development of in-stent neointima. Heart 88, 401–405.CrossRefGoogle ScholarPubMed
Hannan, EL, Racz, M, Holmes, DR, Walford, G, Sharma, S, Katz, S, Jones, RH, King, SB III 2008. Comparison of coronary artery stenting outcomes in the eras before and after the introduction of drug-eluting stents. Circulation 117, 2071–2078.CrossRefGoogle ScholarPubMed
Heldman, AW, Cheng, L, Jenkins, GM, Heller, PF, Kim, DW, Ware, M Jr, Nater, C, Hruban, RH, Rezai, B, Abella, BS, Bunge, KE, Kinsella, JL, Sollott, SJ, Lakatta, EG, Brinker, JA, Hunter, WL, Froehlich, JP 2001. Paclitaxel stent coating inhibits neointimal hyperplasia at 4 weeks in a porcine model of coronary restenosis. Circulation 103, 2289–2295.CrossRefGoogle Scholar
Kingston, PA, Sinha, S, Appleby, CE, David, A, Verakis, T, Castro, MG, Lowenstein, PR, Heagerty, AM 2003. Adenovirus-mediated gene transfer of transforming growth factor-beta3, but not transforming growth factor-beta1, inhibits constrictive remodeling and reduces luminal loss after coronary angioplasty. Circulation 108, 2819–2825.CrossRefGoogle Scholar
Long, X, Bratz, IN, Alloosh, M, Edwards, JM, Sturek, M 2010. Short-term exercise training prevents micro- and macrovascular disease following coronary stenting. Journal of Applied Physiology 108, 1766–1774.CrossRefGoogle ScholarPubMed
Lowe, HC, Schwartz, RS, Mac Neill, BD, Jang, IK, Hayase, M, Rogers, C, Oesterle, SN 2003. The porcine coronary model of in-stent restenosis: current status in the era of drug-eluting stents. Catheterization and Cardiovascular Interventions 60, 515–523.CrossRefGoogle ScholarPubMed
Maxwell, MP, Hearse, DJ, Yellon, DM 1987. Species variation in the coronary collateral circulation during regional myocardial ischaemia: a critical determinant of the rate of evolution and extent of myocardial infarction. Cardiovascular Research 21, 737–746.CrossRefGoogle ScholarPubMed
Posa, A, Nyolczas, N, Hemetsberger, R, Pavo, N, Petnehazy, O, Petrasi, Z, Sangiorgi, G, Gyongyosi, M 2010. Optimization of drug-eluting balloon use for safety and efficacy: evaluation of the 2nd generation paclitaxel-eluting DIOR-balloon in porcine coronary arteries. Catheterization and Cardiovascular Interventions 76, 395–403.CrossRefGoogle ScholarPubMed
Russo, RJ, Silva, PD, Yeager, M 2007. Coronary artery overexpansion increases neointimal hyperplasia after stent placement in a porcine model. Heart 93, 1609–1615.CrossRefGoogle ScholarPubMed
Salem, HK, Ranjzad, P, Driessen, A, Appleby, CE, Heagerty, AM, Kingston, PA 2006. Beta-adrenoceptor blockade markedly attenuates transgene expression from cytomegalovirus promoters within the cardiovascular system. Arteriosclerosis, Thrombbosis and Vascular Biology 26, 2267–2274.CrossRefGoogle ScholarPubMed
Schwartz, RS, Murphy, JG, Edwards, WD, Camrud, AR, Vliestra, RE, Holmes, DR 1990. Restenosis after balloon angioplasty. A practical proliferative model in porcine coronary arteries. Circulation 82, 2190–2200.CrossRefGoogle ScholarPubMed
Schwartz, RS, Edelman, ER, Carter, A, Chronos, N, Rogers, C, Robinson, KA, Waksman, R, Weinberger, J, Wilensky, RL, Jensen, DN, Zuckerman, BD, Virmani, R 2002. Drug-eluting stents in preclinical studies: recommended evaluation from a consensus group. Circulation 106, 1867–1873.CrossRefGoogle ScholarPubMed
Seifert, PS, Huibregtse, BA, Polovick, J, Poff, B 2007. Early vascular response to overlapped paclitaxel-eluting stents in swine coronary arteries. Cardiovascular Revascularization Medicine 8, 251–258.CrossRefGoogle ScholarPubMed
Smith, AC, Swindle, MM 2008. Anesthesia and analgesia in swine, 2nd edition. In Anesthesia and analgesia in laboratory animals (ed. RE Fish, MJ Brown, PJ Danneman and AZ Karas), pp. 413–440. Academic Press, New York.CrossRefGoogle Scholar
Tellez, A, Krueger, CG, Seifert, P, Winsor-Hines, D, Piedrahita, C, Cheng, Y, Milewski, K, Aboodi, MS, Yi, G, McGregor, JC, Crenshaw, T, Reed, JD, Huibregtse, B, Kaluza, GL, Granada, JF 2010. Coronary bare metal stent implantation in homozygous LDL receptor deficient swine induces a neointimal formation pattern similar to humans. Atherosclerosis 213, 518–524.CrossRefGoogle ScholarPubMed
Thim, T, Hagensen, MK, Drouet, L, Bal Dit Sollier, C, Bonneau, M, Granada, JF, Nielsen, LB, Paaske, WP, Botker, HE, Falk, E 2010. Familial hypercholesterolaemic downsized pig with human-like coronary atherosclerosis: a model for preclinical studies. EuroIntervention 6, 261–268.CrossRefGoogle Scholar
Weaver, ME, Pantely, GA, Bristow, JD, Ladley, HD 1986. A quantitative study of the anatomy and distribution of coronary arteries in swine in comparison with other animals and man. Cardiovascular Research 20, 907–917.CrossRefGoogle Scholar
White, FC, Carroll, SM, Magnet, A, Bloor, CM 1992. Coronary collateral development in swine after coronary artery occlusion. Circulation Research 71, 1490–1500.CrossRefGoogle ScholarPubMed