Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-27T19:29:54.632Z Has data issue: false hasContentIssue false
  • English
  • Français

A comparison of two methods for determining titanium dioxide marker content in broiler digestibility studies

Published online by Cambridge University Press:  11 February 2014

N. K. Morgan ,
D. V. Scholey  and
E. J. Burton
N. K. Morgan*
Affiliation:
School of Animal, Rural and Environmental Science, Nottingham Trent University, Southwell, Nottinghamshire, England, NG25 0QF, UK
D. V. Scholey
Affiliation:
School of Animal, Rural and Environmental Science, Nottingham Trent University, Southwell, Nottinghamshire, England, NG25 0QF, UK
E. J. Burton
Affiliation:
School of Animal, Rural and Environmental Science, Nottingham Trent University, Southwell, Nottinghamshire, England, NG25 0QF, UK
*
† E-mail: [email protected]
Get access

Abstract

The use of inert markers in broiler diets eliminates the need to quantitatively evaluate feed intake and excreta output to determine diet digestibility, and enables nutrient uptake at specific points along the gastrointestinal tract to be examined. Titanium dioxide (TiO2) is commonly used for this purpose and measured using a UV-spectrophotometric assay. Two experiments were conducted to observe whether an inductively coupled plasma optical emission spectrophotometer (ICP-OES) assay is able to replace the UV-spectroscopy assay for rapid analysis of TiO2 in broiler feed and ileal digesta samples. In the first experiment, TiO2 was added at 5 g/kg to 19 broiler diets. Ross 308 male broilers (n=452) fed these diets were involved in a series of digestion studies to determine ileal digesta recovery of TiO2. In the second experiment, defined amounts of TiO2 were added to ileal digesta samples from Ross 308 male broilers (n=176) and TiO2 recoveries were determined. The feed and ileal samples from both experiments were analysed by both UV-spectroscopy and ICP-OES, and relatedness of the findings from the two assays was determined. Overall relatedness of the two assays was strong for determination of TiO2 concentration in both the broiler diets and ileal digesta samples (r=0.908 and r=0.884, respectively). Overall recovery of supplemented TiO2 was 97.62% by the UV-spectroscopy assay and 98.77% by the ICP-OES assay. The ICP-OES assay in this study was as accurate as spectrophotometric determination for the quantification of TiO2 content. The ICP-OES method can also be used to analyse several elements within one assay, with a single preparation step, and thus the measurement of TiO2 may be incorporated into the analysis of other minerals. Time and resources dedicated to determining diet digestibility in broilers could be minimised by using the ICP-OES assay to replace the UV-spectroscopy assay when measuring TiO2 concentration.

Keywords

broilertitanium dioxidedigestibilitymethodology
Type
Full Paper
Information
animal , Volume 8 , Issue 4 , April 2014 , pp. 529 - 533
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Boguhn, J, Baumgärtel, T, Dieckmann, A and Rodehutscord, M 2009. Reaserch note: determination of titanium dioxide supplements in different matrices using two methods involving photometer and inductively coupled plasma optical emission spectrometer measurements. Archives of Animal Nutrition 63, 337–342.Google Scholar
Cheng, TK and Coon, CN 1990. Research note: calcium digestibility studies utilizing acid-insoluble ash measurements. Poultry Science 69, 2228–2230.Google Scholar
Cohen, JW 1988. Statistical power analysis for the behavioural sciences, 2nd edition, Lawrence Erlbaum Associates, Hillsdale, NJ.Google Scholar
Jagger, S, Wiseman, J, Cole, DJA and Craigon, J 1992. Evaluation of inert markers for the determination of ileal and faecal apparent digestibility values in the pig. British Journal of Nutrition 68, 729–739.Google Scholar
Myers, WD, Ludden, PA, Nayigihugu, V and Hess, BW 2004. Technical note: a procedure for the preparation and quantitative analysis of samples for titanium dioxide. Journal of Animal Science 82, 179–183.Google Scholar
Rodehutscord, M, Dieckmann, A, Witzig, M and Shastak, Y 2012. A note on sampling digesta from the ileum of broilers in phosphorus digestibility studies. Poultry Science 91, 965–971.Google Scholar
Rutherfurd, SM, Chung, TK, Morel, PCH and Moughan, PJ 2004. Effect of microbial phytase on ileal digestibility of phytate phosphorus, total phosphorus, and amino acids in a low-phosphorus diet for broilers. Poultry Science 83, 61–68.Google Scholar
Short, FJ, Gorton, P and Wiseman, J 1996. Determination of titanium dioxide added as an inert marker in chicken digestibility studies. Animal Feed Science and Technology 59, 215–221.Google Scholar
Titgemeyer, EC, Armendariz, CK, Bindel, DJ, Greenwood, RH and Löest, CA 2001. Evaluation of titanium dioxide as a digestibility marker for cattle. Journal of Animal Science 79, 1059–1063.CrossRefGoogle ScholarPubMed
Walk, CL, Bedford, MR and McElroy, AP 2012. Influence of limestone and phytase on broiler performance, gastrointestinal pH, and apparent ileal nutrient digestibility. Poultry Science 91, 1371–1378.CrossRefGoogle ScholarPubMed