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Characterisation of white and black merino wools: a proteomics study

Published online by Cambridge University Press:  09 July 2018

J. Plowman ,
A. Thomas ,
T. Perloiro ,
S. Clerens  and
A. M. de Almeida Open the ORCID record for A. M. de Almeida [Opens in a new window]
J. Plowman
Affiliation:
AgResearch Ltd, 1365 Springs Road, Lincoln 7674, Private Bag Christchurch 8140, Christchurch, New Zealand
A. Thomas
Affiliation:
AgResearch Ltd, 1365 Springs Road, Lincoln 7674, Private Bag Christchurch 8140, Christchurch, New Zealand
T. Perloiro
Affiliation:
ANCORME – Associação Nacional de Criadores de Raça Ovina Merina, 7005-873 Évora, Portugal
S. Clerens
Affiliation:
AgResearch Ltd, 1365 Springs Road, Lincoln 7674, Private Bag Christchurch 8140, Christchurch, New Zealand
A. M. de Almeida
Affiliation:
Ross University School of Veterinary Medicine, PO Box 334, Basseterre, St. Kitts and Nevis, West Indies
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Abstract

Wool is an important agricultural commodity with merino wool being rated alongside the finest quality fibres, which include the goat fibres Mohair and Cashmere. Although pigmented wool merinos have become extremely rare, the market for this wool is increasing. In Portugal, there are two merino breeds: white and black, descendants of animals originally bred on the Iberian Peninsula. These breeds have the potential to assist in our understanding of how protein expression relates to wool traits of importance to the textile industry. Herein, we study the characteristics and protein expression profiles of wool from ewes of the Portuguese black and white merino (n=15). Both breeds had very similar results for fibre diameter (25 µm) and curvature (105 to 111°/mm). Significant between-breed differences were found in the two types of keratin-associated proteins (KAPs): high-sulphur proteins (HSPs) and high-glycine–tyrosine proteins (HGTPs). The expression of HSPs, KAP2-3 and KAP2-4, decreased expression in the pigmented animals, whereas KAP13-1 was found in higher amounts. Likewise, the expression of the ultra-high-sulphur proteins, KAP4-3 and KAP4-7-like, was reduced in black sheep to half the levels of the white wools, whereas the HGTPs, KAP6, KAP6-1, KAP6-2 and KAP16-2, were more abundant in black sheep. These results suggest structural differences between the black and white merino wool, because of differences among some KAPs. These differences have important implications for the textile industry.

Keywords

proteomefibre qualityhigh-sulphur proteinshigh-glycine–tyrosine proteinskeratin
Type
Research Article
Information
animal , Volume 13 , Issue 3 , March 2019 , pp. 659 - 665
Copyright
© The Animal Consortium 2018 

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Footnotes

†

Present address: Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda 1349-017 Lisboa, Portugal. E-mail: [email protected]

References

Allain, D and Renieri, C 2010. Genetics of fibre production and fleece characteristics in small ruminants, Angora rabbit and South American camelids. Animal 4, 1472–1481.Google Scholar
Almeida, AM, Bassols, A, Bendixen, E, Bhide, M, Ceciliani, F, Cristobal, S, Eckersall, PD, Hollung, K, Lisacek, F, Mazzucchelli, G, McLaughlin, M, Miller, I, Nally, JE, Plowman, J, Renaut, J, Rodrigues, P, Roncada, P, Staric, J and Turk, R 2015. Animal board invited review: advances in proteomics for animal and food sciences. Animal 9, 1–17.Google Scholar
Almeida, AM, Palhinhas, RG, Kilminster, T, Scanlon, T, van Harten, S, Milton, J, Blache, D, Greeff, J, Oldham, C, Coelho, AV and Cardoso, LA 2016. The effect of weight loss on the muscle proteome in the Damara, Dorper and Australian Merino Ovine breeds. PLoS One 11, e0146367.Google Scholar
Almeida, AM, Plowman, JE, Harland, DP, Thomas, A, Kilminster, T, Scanlon, T, Milton, J, Greeff, J, Oldham, C and Clerens, S 2014. Influence of feed restriction on the wool proteome: a combined iTRAQ and fiber structural study. Journal of Proteomics 103, 170–177.Google Scholar
Campbell, ME, Whitely, KJ and Gillespie, JM 1972. Compositional studies of high- and low crimp wools. Australian Journal of Biological Sciences 25, 977–987.Google Scholar
Campbell, ME, Whiteley, KJ and Gillespie, JM 1975. Influence of nutrition on the crimping rate of wool and the type and proportion of constituent proteins. Australian Journal of Biological Sciences 28, 389–397.Google Scholar
Ciani, E, Lasagna, E, D’Andrea, M, Alloggio, I, Marroni, F, Ceccobelli, S, Delgado Bermejo, JV, Sarti, FM, Kijas, J, Lenstra, JA and Pilla, F, International Sheep Genomics Consortium 2015. Merino and Merino-derived sheep breeds: a genome-wide intercontinental study. Genetetics Selection Evolution 14, 47–64.Google Scholar
Flanagan, LM, Plowman, JE and Bryson, WG 2002. The high sulphur proteins of wool: towards an understanding of sheep breed diversity. Proteomics 2, 1240–1246.Google Scholar
Fraser, RDB and Rogers, GE 1955. The bilateral structure of wool cortex and its relationship to crimp. Australian Journal of Biological Sciences 8, 288–289.Google Scholar
Fratini, A, Powell, BC, Hynd, PI, Keough, RA and Rogers, GE 1994. Dietary cysteine regulates the levels of mRNAs encoding a family of cysteine-rich proteins of wool. Journal of Investigative Dermatology 102, 178–185.Google Scholar
Fratini, A, Powell, BC and Rogers, GE 1993. Sequence, expression, and evolutionary conservation of a gene encoding a glycine/tyrosine-rich keratin-associated protein of hair. Journal of Biological Chemistry 268, 4511–4518.Google Scholar
Fujikawa, H, Fujimoto, A, Farooq, M, Ito, M and Shimomura, S 2012. Characterisation of the human hair keratin-associated protein 2 (KRTAP2) gene family. Journal Investigative Dermatolology 132, 1806–1813.Google Scholar
Fujimoto, S, Takase, T, Kadono, N, Maekubo, K and Hirai, Y 2014. Krtap11-1, a hair keratin-associated protein, as a possible crucial element for the physical properties of hair shafts. Journal Dermatological Science 74, 39–47.Google Scholar
Koehn, H, Clerens, S, Deb-Choudhury, S, Morton, JD, Dyer, JM and Plowman, JE 2009. Higher sequence coverage and improved confidence in the identification of cysteine-rich proteins from the wool cuticle using combined chemical and enzymatic digestion. Journal of Proteomics 73, 323–330.Google Scholar
Koehn, H, Clerens, S, Deb-Choudhury, S, Morton, JD, Dyer, JM and Plowman, JE 2010. The proteome of the wool cuticle. Journal of Proteome Research 9, 2920–2928.Google Scholar
Li, Y, Zhou, G, Zhang, R, Guo, J, Li, C, Martin, G, Chen, Y and Wang, X 2018. Comparative proteomic analyses using iTRAQ-labeling provides insights into fiber diversity in sheep and goats. Journal of Proteomics 172, 82–88.Google Scholar
Littel, RC, Freund, RJ and Spector, PC 1991. SAS system for linear models, 3rd edition. Statistical Analysis Systems Institute Inc, Cary, NC, USA.Google Scholar
Matos, CAP 2000. Animal genetic resources and traditional production systems in Portugal. Archivos de Zootecnia 49, 363–383.Google Scholar
Orwin, DFG, Woods, JL and Ranford, SL 1984. Cortical cell types and their distribution in wool fibres. Australian Journal of Biological Sciences 37, 237–255.Google Scholar
Pereira, F, Davis, SJM, Pereira, L, McEvoy, B, Bradley, DG and Amorim, A 2006. Genetic signatures of a Mediterranean influence in Iberian Peninsula sheep husbandry. Molecular Biolology Evolution 23, 1420–1426.Google Scholar
Plowman, JE, Bryson, WG and Jordan, TW 2000. Application of proteomics for determining protein markers for wool quality traits. Electrophoresis 21, 1899–1906.Google Scholar
Plowman, JE, Deb-Choudhury, S, Clerens, S, Thomas, A, Cornellison, CD and Dyer, JM 2012. Unravelling the proteome of wool: towards markers of wool quality traits. Journal of Proteomics 75, 4315–4324.Google Scholar
Plowman, JE, Flanagan, LM, Paton, LN, Fitzgerald, AC, Joyce, NI and Bryson, WG 2003. The effect of oxidation or alkylation on the separation of wool keratin proteins by two-dimensional gel electrophoresis. Proteomics 3, 942–950.Google Scholar
Plowman, JE, Harland, DP, Ganeshan, S, Woods, JL, van Shaijik, B, Deb-Choudhury, S, Thomas, A, Clerens, S and Scobie, DR 2015. The proteomics of wool fibre morphogenesis. Journal of Structural Biology 191, 341–351.Google Scholar
Powell, BC, Nesci, A and Rogers, GE 1991. Regulation of keratin gene expression in hair follicle differentiation. Annals NY Academy of Sciences 642, 1–20.Google Scholar
Rogers, MA, Langbein, L, Winter, H, Ehmann, C, Praetzel, S, Korn, B and Schweizer, J 2001. Characterisation of a cluster of human high/ultrahigh sulphur keratin-associated protein genes embedded in the type I keratin gene domain on chromosome 17q12-21. Journal of Biological Chemistry 276, 19440–19451.Google Scholar
Rogers, MA, Langbein, L, Winter, H, Ehmann, C, Praetzel, S and Schweizer, J 2002. Characterisation of a first domain of human high glycine-tyrosine and high sulphur keratin-associated protein (KAP) genes on chromosome 21q22.1. Journal of Biological Chemistry 277, 48993–49002.Google Scholar
Soares, R, Franco, C, Pires, E, Ventosa, M, Palhinhas, R, Koci, K, Martinho de Almeida, A and Varela Coelho, A 2012. Mass spectrometry and animal science: protein identification strategies and particularities of farm animal species. Journal of Proteomics 75, 4190–4206.Google Scholar
Yu, Z, Gordon, SW, Nixon, AJ, Bawden, CS, Rogers, MA, Wildermoth, JE, Maqbool, NJ and Pearson, AJ 2009. Expression patterns of keratin intermediate filament and keratin associated protein genes in wool follicles. Differentiation 77, 307–316.Google Scholar
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