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Characterisation of haematological profiles and whole blood relative gene expression levels in Holstein-Friesian and Jersey bull calves undergoing gradual weaning

Published online by Cambridge University Press:  20 November 2015

D. Johnston ,
D. A. Kenny ,
A. K. Kelly ,
M. S. McCabe ,
M. McGee ,
S. M. Waters  and
B. Earley
D. Johnston
Affiliation:
Animal & Grassland Research and Innovation Centre, Teagasc, Dunsany, Co. Meath C15 PW93, Ireland University College Dublin, College of Agriculture Food Science and Veterinary Medicine, Dublin, Belfield, Dublin 4, Ireland
D. A. Kenny
Affiliation:
Animal & Grassland Research and Innovation Centre, Teagasc, Dunsany, Co. Meath C15 PW93, Ireland
A. K. Kelly
Affiliation:
University College Dublin, College of Agriculture Food Science and Veterinary Medicine, Dublin, Belfield, Dublin 4, Ireland
M. S. McCabe
Affiliation:
Animal & Grassland Research and Innovation Centre, Teagasc, Dunsany, Co. Meath C15 PW93, Ireland
M. McGee
Affiliation:
Animal & Grassland Research and Innovation Centre, Teagasc, Dunsany, Co. Meath C15 PW93, Ireland
S. M. Waters
Affiliation:
Animal & Grassland Research and Innovation Centre, Teagasc, Dunsany, Co. Meath C15 PW93, Ireland
B. Earley*
Affiliation:
Animal & Grassland Research and Innovation Centre, Teagasc, Dunsany, Co. Meath C15 PW93, Ireland
*
† E-mail: [email protected]
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Abstract

Haematological profiles indicate the health status of an animal and can be used to identify sub-clinical stress responses. The objectives of the study were to examine (i) the effect of breed and plane of nutrition, on haematological profiles of artificially reared Holstein-Friesian and Jersey bull calves in response to gradual weaning, and (ii) the effect of breed on immune response genes in bovine whole blood using real-time quantitative PCR. Holstein-Friesian and Jersey bull calves were group housed indoors and individually fed using an automatic feeder. They were allocated to a high, medium or low plane of nutrition, based on milk replacer (MR) and concentrate. The nutrition treatments were calculated using National Research Council guidelines in order to achieve a high, medium or low growth rate for each respective breed. During the weaning phase MR was gradually reduced over a 14-day (d) period (d −13 to d 0). Calves were blood sampled on d −14, −6, −3, 0, 1, 3, 8 and 14 relative to weaning (d 0) for subsequent haematological analysis. On d −14, 1 and 8, a subset of eight Holstein-Friesian calves randomly selected from the medium nutrition treatment and eight Jersey calves randomly selected from the high nutrition treatment, were blood sampled for gene expression profiling, targeting biomarkers of weaning stress. These two treatment groups were chosen to examine the effect of breed on expression of the genes of interest, as energy intake and animal performance were similar. There was no effect of breed×plane of nutrition interaction nor effect of plane of nutrition on any variable measured (P>0.05). Gradual weaning produced differential biological responses in the two breeds evidenced by breed×time interactions for lymphocyte, monocyte and red blood cell number, plasma haemoglobin and haptoglobin concentrations (P<0.05). The typical stress response consisting of neutrophilia and lymphopaenia was not observed for any treatment. An immune response to gradual weaning was observed as the relative gene expression level of the pro-apoptotic gene, Fas, increased on d 1 relative to d −14 (P<0.05). Relative gene expression levels were greater in Jersey calves compared with Holstein-Friesian for the pro-inflammatory cytokine CXCL8 (P=0.05) and the glucocorticoid receptor, GRα (P<0.05). The increased levels of these transcripts suggest that Jersey calves may have a more sensitive immune system compared with Holstein-Friesian.

Keywords

breeddairy calveshaematologyplane of nutritionweaning
Type
Research Article
Information
animal , Volume 10 , Supplement 9 , September 2016 , pp. 1547 - 1556
Copyright
© The Animal Consortium 2015 

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References

Bach, A, Terré, M and Pinto, A 2013. Performance and health responses of dairy calves offered different milk replacer allowances. Journal of Dairy Science 96, 7790–7797.Google Scholar
Ballou, MA 2012. Immune responses of Holstein and Jersey calves during the preweaning and immediate postweaned periods when fed varying planes of milk replacer. Journal of Dairy Science 95, 7319–7330.Google Scholar
Ballou, MA, Cobb, CJ, Earleywine, TJ and Obeidat, BS 2013. Breed and plane of milk-replacer nutrition influence the performance of pre- and postweaned dairy calves. The Professional Animal Scientist 29, 116–123.Google Scholar
Borderas, TF, de Passillé, AMB and Rushen, J 2009. Feeding behavior of calves fed small or large amounts of milk. Journal of Dairy Science 92, 2843–2852.Google Scholar
Budzynska, M and Weary, DM 2008. Weaning distress in dairy calves: effects of alternative weaning procedures. Applied Animal Behaviour Science 112, 33–39.CrossRefGoogle Scholar
Bustin, S, Benes, V, Garson, J, Hellemans, J, Huggett, J, Kubista, M, Mueller, R, Nolan, T, Pfaffl, M and Shipley, G 2009. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 55, 611–622.Google Scholar
Conneely, M, Berry, DP, Murphy, JP, Lorenz, I, Doherty, ML and Kennedy, E 2014. Effects of milk feeding volume and frequency on body weight and health of dairy heifer calves. Livestock Science 161, 90–94.Google Scholar
DAFM 2013. AIM bovine statistics report 2013. Retrieved November 1, 2014, from https://www.agriculture.gov.ie/media/migration/animalhealthwelfare/animalidentificationandmovement/cattlemovementmonitoringsystem/AIMBOVINESTATISTICS2013050614.pdf.Google Scholar
Dhakal, K, Maltecca, C, Cassady, JP, Baloche, G, Williams, CM and Washburn, SP 2013. Calf birth weight, gestation length, calving ease, and neonatal calf mortality in Holstein, Jersey, and crossbred cows in a pasture system. Journal of Dairy Science 96, 690–698.CrossRefGoogle Scholar
Earley, B, McGee, M, Fallon, RJ, Drennan, MJ, Murray, M and Farrell, JA 2000. Serum immunoglobulin concentrations in suckled calves and dairy-herd calves. Irish Journal of Agricultural and Food Research 39, 401–407.Google Scholar
Eckersall, P, Duthie, S, Safi, S, Moffatt, D, Horadagoda, N, Doyle, S, Parton, R, Bennett, D and Fitzpatrick, J 1999. An automated biochemical assay for haptoglobin: prevention of interference from albumin. Comparative Haematology International 9, 117–124.CrossRefGoogle Scholar
Foote, MR, Nonnecke, BJ, Beitz, DC and Waters, WR 2007. High growth rate fails to enhance adaptive immune responses of neonatal calves and is associated with reduced lymphocyte viability. Journal of Dairy Science 90, 404–417.CrossRefGoogle ScholarPubMed
Goossens, K, Van Poucke, M, Van Soom, A, Vandesompele, J, Van Zeveren, A and Peelman, L 2005. Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC Developmental Biology 5, 27.CrossRefGoogle ScholarPubMed
Hickey, M, Drennan, M and Earley, B 2003. The effect of abrupt weaning of suckler calves on the plasma concentrations of cortisol, catecholamines, leukocytes, acute-phase proteins and in vitro interferon-gamma production. Journal of Animal Science 81, 2847–2855.Google Scholar
Hodgson, P, Aich, P, Stookey, J, Popowych, Y, Potter, A, Babiuk, L and Griebel, P 2012. Stress significantly increases mortality following a secondary bacterial respiratory infection. Veterinary Research 43, 21.Google Scholar
Hoffmann, E, Dittrich-Breiholz, O, Holtmann, H and Kracht, M 2002. Multiple control of interleukin-8 gene expression. Journal of Leukocyte Biology 72, 847–855.Google Scholar
Jasper, J and Weary, DM 2002. Effects of ad libitum milk intake on dairy calves. Journal of Dairy Science 85, 3054–3058.CrossRefGoogle ScholarPubMed
Jones, M and Allison, R 2007. Evaluation of the ruminant complete blood cell number. The Veterinary Clinics of North America. Food Animal Practice 23, 377–402.CrossRefGoogle Scholar
Khan, MA, Lee, HJ, Lee, WS, Kim, HS, Kim, SB, Ki, KS, Ha, JK, Lee, HG and Choi, YJ 2007. Pre- and postweaning performance of Holstein female calves fed milk through step-down and conventional methods. Journal of Dairy Science 90, 876–885.CrossRefGoogle ScholarPubMed
Kim, MH, Yang, JY, Upadhaya, SD, Lee, HJ, Yun, CH and Ha, JK 2011. The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves. Journal of Veterinary Science 12, 151–157.Google Scholar
Koper, JW, Van Rossum, EFC and Van Den Akker, ELT 2014. Glucocorticoid receptor polymorphisms and haplotypes and their expression in health and disease. Steroids 92, 62–73.CrossRefGoogle ScholarPubMed
Lorenz, I, Mee, J, Earley, B and More, S 2011. Calf health from birth to weaning. I. General aspects of disease prevention. Irish Veterinary Journal 64, 10.CrossRefGoogle ScholarPubMed
Lynch, E, Earley, B, McGee, M and Doyle, S 2010. Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves. BMC Veterinary Research 6, 39.Google Scholar
McEwan, AD, Fisher, EW, Selman, IE and Penhale, WJ 1970. A turbidity test for the estimation of immune globulin levels in neonatal calf serum. Clinica Chimica Acta 27, 155–163.CrossRefGoogle ScholarPubMed
Muller, LD and Ellinger, DK 1981. Colostral immunoglobulin concentrations among breeds of dairy cattle. Journal of Dairy Science 64, 1727–1730.Google Scholar
Murata, H, Shimada, N and Yoshioka, M 2004. Current research on acute phase proteins in veterinary diagnosis: an overview. Veterinary Journal 168, 28–40.Google Scholar
National Research Council (NRC) 2001. Nutrient requirements of dairy cattle, 7th edition. National Academy of Sciences, Washington, DC, USA.Google Scholar
Nielsen, PP, Jensen, MB and Lidfors, L 2008. Milk allowance and weaning method affect the use of a computer controlled milk feeder and the development of cross-sucking in dairy calves. Applied Animal Behaviour Science 109, 223–237.Google Scholar
Obeidat, BS, Cobb, CJ, Sellers, MD, Pepper-Yowell, AR, Earleywine, TJ and Ballou, MA 2013. Plane of nutrition during the preweaning period but not the grower phase influences the neutrophil activity of Holstein calves. Journal of Dairy Science 96, 1–12.CrossRefGoogle Scholar
O’Loughlin, A, McGee, M, Doyle, S and Earley, B 2014. Biomarker responses to weaning stress in beef calves. Research in Veterinary Science 97, 458–463.Google Scholar
O’Loughlin, A, McGee, M, Waters, S, Doyle, S and Earley, B 2011. Examination of the bovine leukocyte environment using immunogenetic biomarkers to assess immunocompetence following exposure to weaning stress. BMC Veterinary Research 7, 45.Google Scholar
Oura, R, Arakaki, R, Yamada, A, Kudo, Y, Tanaka, E, Hayashi, Y and Ishimaru, N 2013. Induction of rapid T cell death and phagocytic activity by Fas-deficient lpr macrophages. The Journal of Immunology 190, 578–585.Google Scholar
Pfaffl, M 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 29, e45.CrossRefGoogle ScholarPubMed
Sapolsky, R, Romero, L and Munck, A 2000. How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endocrine Reviews 21, 55–89.Google Scholar
Spalenza, V, Girolami, F, Bevilacqua, C, Riondato, F, Rasero, R, Nebbia, C, Sacchi, P and Martin, P 2011. Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes. The Veterinary Journal 189, 278–283.Google Scholar
Sweeney, BC, Rushen, J, Weary, DM and de Passillé, AM 2010. Duration of weaning, starter intake, and weight gain of dairy calves fed large amounts of milk. Journal of Dairy Science 93, 148–152.Google Scholar
Terré, M, Devant, M and Bach, A 2007. Effect of level of milk replacer fed to Holstein calves on performance during the preweaning period and starter digestibility at weaning. Livestock Science 110, 82–88.Google Scholar
Villarroel, A, Miller, TB, Johnson, ED, Noyes, KR and Ward, JK 2013. Factors affecting serum total protein and immunoglobulin G concentration in replacement dairy calves. Advances in Dairy Research 1, 106.Google Scholar
Viola, A and Luster, AD 2008. Chemokines and their receptors: drug targets in immunity and inflammation. Annual Review of Pharmacology and Toxicology 48, 171–197.Google Scholar
Weary, DM, Jasper, J and Hötzel, MJ 2008. Understanding weaning distress. Applied Animal Behaviour Science 110, 24–41.Google Scholar
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