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Bilberry pomace in rabbit nutrition: effects on growth performance, apparent digestibility, caecal traits, bacterial community and antioxidant status

Published online by Cambridge University Press:  18 May 2018

S. Dabbou ,
I. Ferrocino ,
A. Kovitvadhi ,
S. Dabbou ,
S. Bergagna ,
D. Dezzuto ,
A. Schiavone ,
L. Cocolin ,
F. Gai  and
V. Santoro
...Show all authors
S. Dabbou
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, Italy Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, Italy
I. Ferrocino
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, Italy
A. Kovitvadhi
Affiliation:
Department of Physiology, Kasetsart University, 50 Ngam Wong Wan Road, Chatuchak, 10900 Bangkok, Thailand
S. Dabbou
Affiliation:
Dentistry Faculty, University of Monastir, Avicenne Street, 5019 Monastir, Tunisia
S. Bergagna
Affiliation:
Veterinary Medical Research Institute for Piemonte, Liguria and Valle d’Aosta, Via Bologna 148, 10154, Turin, Italy
D. Dezzuto
Affiliation:
Veterinary Medical Research Institute for Piemonte, Liguria and Valle d’Aosta, Via Bologna 148, 10154, Turin, Italy
A. Schiavone
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, Italy Institute of Science of Food Production, National Research Council, Largo P. Braccini 2, 10095 Grugliasco, Italy
L. Cocolin
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, Italy
F. Gai*
Affiliation:
Institute of Science of Food Production, National Research Council, Largo P. Braccini 2, 10095 Grugliasco, Italy
V. Santoro
Affiliation:
Department of Molecular Biotechnology and Health Sciences, University of Turin, Via P. Giuria 5, 10125, Turin, Italy
L. Gasco
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, Italy Institute of Science of Food Production, National Research Council, Largo P. Braccini 2, 10095 Grugliasco, Italy
*
†E-mail: [email protected]
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Abstract

Agricultural by-products could be used as alternative raw materials in rabbit nutrition as they have been found to be highly nutritious and low cost feeding sources. The aim of this study was to estimate the nutritive value and potential use of bilberry pomace (BP) for growing rabbits. A total of 144 Grimaud rabbits (35 days old) were allotted to four groups and fed with a diet containing increasing level of BP: BP0 (basal diet), BP5, BP10 and BP15 containing 0, 50, 100 and 150 g/kg respectively. Growth trial lasted 48 days; apparent digestibility was evaluated, starting at 46 days of age, over 4 consecutive days. The nutritive value of BP was measured using the mean digestibility of the experimental diets. At 83 days of age, rabbits were slaughtered: blood, and liver and kidney samples were collected in order to determine the blood parameters and the antioxidant enzyme activities of the tissues. Moreover, caecal content was sampled and gut microbiota assessed by means of amplicon-based high-throughput 16S rRNA sequencing and PCR-denaturing gradient gel electrophoresis. The digestible protein was estimated to 104 g/kg of DM while digestible energy to 9.44 MJ/kg DM for incorporation rate up to 150 g/kg. During the finishing period, average daily feed intake and feed conversion ratio showed linear response to BP increase (P=0.008 and <0.001, respectively). During all the period, both parameters decreased linearly and quadratically with increasing BP inclusion levels (P<0.001) up to 100 g/kg of BP. A significant effect of the antioxidant status was found in the kidneys and liver (P<0.05) where the glutathione peroxidase activity increased as the BP increased. As far as gut microbiota is concerned, BP increased the relative abundance of the Clostridium, Oscillospira, Ruminococcus and Ruminococcaceae species which were clearly associated with the BP inclusion level. In conclusion, BP showed a potential use as an alternative protein and fibre sources for growing rabbits.

Keywords

feedsVaccinium myrtillusby-productnutritive valuemicrobiota
Type
Research Article
Information
animal , Volume 13 , Issue 1 , January 2019 , pp. 53 - 63
Copyright
© The Animal Consortium 2018 

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References

Association of Official Analytical Chemists (AOAC) 2000. Official methods of analysis, 16th edition. AOAC, Gaithersburg, MD, USA.Google Scholar
Association of Official Analytical Chemists (AOAC) 2003. Official methods of analysis, 17th edition. AOAC, Gaithersburg, MD, USA.Google Scholar
Choi, ChS, Chung, HK, Choi, MK and Kang, MH 2010. Effects of grape pomace on the antioxidant defense system in diet-induced hypercholesterolemic rabbits. Nutrition Research and Practice 4, 114–120.Google Scholar
Chung, H, Pamp, SJ, Hill, JA, Surana, NK, Edelman, SM, Troy, EB, Reading, NC, Villablanca, EJ, Wang, S, Mora, JR, Umesaki, Y, Mathis, D, Benoist, C, Relman, DA and Kasper, DL 2012. Gut immune maturation depends on colonization with a host specific microbiota. Cell 149, 1578–1593.Google Scholar
Combes, S, Fortun-Lamothe, L, Cauquil, L and Gidenne, T 2013. Engineering the rabbit digestive ecosystem to improve digestive health and efficacy. Animal 7, 1429–1439.Google Scholar
Dabbou, S, Gasco, L, Gai, F, Zoccarato, I, Rotolo, L, Dabbou Fekih, S, Brugiapaglia, A, Helal, A N and Peiretti, PG 2014. Dried artichoke bracts in rabbits nutrition: effects on the carcass characteristics, meat quality and fatty-acid composition. Animal 8, 1547–1553.Google Scholar
Dabbou, S, Gai, F, Renna, M, Rotolo, L, Dabbou, S, Lussiana, C, Kovitvadhi, A, Brugiapaglia, A, De Marco, M, Helal, AN, Zoccarato, I and Gasco, L 2017a. Inclusion of bilberry pomace in rabbit diets: effects on carcass characteristics and meat quality. Meat Science 124, 77–83.Google Scholar
Dabbou, S, Renna, M, Lussiana, C, Gai, F, Rotolo, L, Kovitvadhi, A, Brugiapaglia, A, Helal, AN, Schiavone, A, Zoccarato, I and Gasco, L 2017b. Bilberry pomace in growing rabbit diets: effects on quality traits of hind leg meat. Italian Journal of Animal Science 16, 371–379.Google Scholar
De Filippis, F, Pellegrini, N, Vannini, L, Jeffery, IB, La Storia, A, Laghi, L, Serrazanetti, DI, Di Cagno, R, Ferrocino, I, Lazzi, C, Turroni, S, Cocolin, L, Brigidi, P, Neviani, E, Gobbetti, M, O’Toole, PW and Ercolini, D 2015. High-level adherence to a Mediterranean diet beneficially impacts the gut microbiota and associated metabolome. Gut Microbiota 0, 1–10.Google Scholar
European Group on Rabbit Nutrition (EGRAN) 2001. Technical note: attempts to harmonize chemical analyses of feeds and faeces, for rabbit feed evaluation. World Rabbit Science 9, 57–64.Google Scholar
Ferrocino, I, Bellio, A, Romano, A, Macori, G, Rantsiou, K, Decastelli, L and Cocolin, L 2017. RNA-based amplicon sequencing reveals microbiota development during ripening of artisanal versus industrial lard d’Arnad. Applied and Environmental Microbiology 83, e00983–17.Google Scholar
Ferrocino, I, Di Cagno, R, De Angelis, M, Turroni, S, Vannini, L, Bancalari, E, Rantsiou, K, Cardinali, G, Neviani, E and Cocolin, L 2015. Fecal microbiota in healthy subjects following omnivore, vegetarian and vegan diets: culturable populations and rRNA DGGE profiling. PLoS One 10, 1–16.Google Scholar
Garcia, G, Galvez, JF and de Blas, JC 1993. Effect of substitution of sugarbeet pulp for barley in diets for finishing rabbits on growth performance and on energy and nitrogen efficiency. Journal of Animal Science 71, 1823–1830.Google Scholar
Gidenne, T, Carabaño, R, García, J and De Blas, JC 2010. Fibre Digestion. In The nutrition of the rabbits, 2nd edition (ed. JC De Blas and J Wiseman), pp 66–82. CABI International, Wallingford, UK.Google Scholar
Guemour, D, Bannelier, C, Dellal, A and Gidenne, T 2010. Nutritive value of sun-dried grape pomace, incorporated at a low level in complete feed for the rabbit bred under Magrebian conditions. World Rabbit Science 18, 17–25.Google Scholar
INRA 2004. Tables de composition et de valeur nutritive des matières premières destinées aux animaux d’élevage: porc, volailles, bovins, ovins, caprins, lapins, chevaux, poissons. (ed. D Sauvant, JM Perez and G Tran), 2nd edition. p. 301. INRA Editions, Paris, France.Google Scholar
Jankowski, J, Juśkiewicz, J, Zduńczyk, P, Kosmala, M, Zieliński, H, Antoszkiewicz, Z and Zduńczyk, Z 2016. Antioxidant status of blood and liver of turkeys fed diets enriched with polyunsaturated fatty acids and fruit pomaces as a source of polyphenols. Polish Journal of Veterinary Sciences 19, 89–98.Google Scholar
Jurgoński, A, Juśkiewicz, J, Zduńczyk, Z, Matusevicius, P and Kołodziejczyk, K 2014. Polyphenol-rich extract from blackcurrant pomace attenuates the intestinal tract and serum lipid changes induced by a high-fat diet in rabbits. European Journal of Nutrition 53, 1603–1613.Google Scholar
Juskiewicz, J, Jankowski, J, Kosmala, M, Zdunczyk, Z, Slominski, BA and Zdunczyk, P 2016. The effects of dietary dried fruit pomaces on growth performance and gastrointestinal biochemistry of turkey poults. Journal of Animal Physiology and Animal Nutrition 100, 967–976.Google Scholar
Khanal, RC, Howard, LR, Wilkes, SE, Rogers, TJ and Prior, RL 2012. Effect of dietary blueberry pomace on selected metabolic factors associated with high fructose feeding in growing Sprague-dawley rats. Journal of Medicinal Food 15, 802–810.Google Scholar
Kovitvadhi, A, Gai, F, Dabbou, S, Ferrocino, I, Rotolo, L, Falzone, M, Vignolini, C, Gennero, MS, Bergagna, S, Dezzutto, D, Barbero, R, Nebbia, P, Rosati, S, Cocolin, L, Zoccarato, I and Gasco, L 2016. Rabbit dietary supplementation with pale purple coneflower. 2. Effects on the performances, bacterial community, blood parameters and immunity of growing rabbits. Animal 10, 1110–1117.Google Scholar
Martens, L, Perez, JM, Villamide, M, Cervera, C, Gidenne, T and Xiccato, G 2002. Nutritive value of raw materials for rabbits: EGRAN tables 2002. World Rabbit Science 10, 157–166.Google Scholar
Michelland, RJ, Combes, S, Monteils, V, Cauquil, L, Gidenne, T and Fortun-Lamothe, L 2010. Molecular analysis of the bacterial community in digestive tract of rabbit. Anaerobe 16, 61–65.Google Scholar
Nguemeni, C, Gouix, E, Bourourou, M, Heurteaux, C and Blondeau, N 2013. Alpha- linolenic acid: a promising nutraceutical for the prevention of stroke. Pharma Nutrition 1, 1–8.Google Scholar
Pascual, JJ, Motta, W, Cervera, C, Quevedo, F, Blas, E and Fernández-Carmona, J 2002. Effect of dietary energy source on the performance and perirenal fat thickness evolution of primiparous rabbit does. Animal Science 75, 267–279.Google Scholar
Pieszka, M, Szczurek, P, Bederska-Łojewska, D, Migdał, W, Pieszka, M, Gogol, P and Jagusiak, W 2017. The effect of dietary supplementation with dried fruit and vegetable pomaces on production parameters and meat quality in fattening pigs. Meat Science 126, 1–10.Google Scholar
Ponnusamy, K, Choi, JN, Kim, J, Lee, SY and Lee, CH 2011. Microbial community and metabolomic comparison of irritable bowel syndrome faeces. Journal of Medical Microbiology 60, 817–827.Google Scholar
Puupponen-Pimiä, R, Nohynek, L, Hartmann-Schmidlin, S, Kähkönen, M, Heinonen, M, Määttä-Riihinen, K and Oksman-Caldentey, KM 2005. Berry phenolics selectively inhibit the growth of intestinal pathogens. Journal of Applied Microbiology 98, 991–1000.Google Scholar
Reichardt, N, Duncan, SH, Young, P, Belenguer, A, McWilliam Leitch, C, Scott, KP, Flint, HJ and Louis, P 2014. Phylogenetic distribution of three pathways for propionate production within the human gut microbiota. The ISME Journal 8, 1323–1335.Google Scholar
Rotolo, L, Gai, F, Peiretti, P G, Ortoffi, M, Zoccarato, I and Gasco, L 2014. Live yeast (Saccharomyces cerevisiae var. boulardii) supplementation in fattening rabbit diet: effect on productive performance and meat quality. Livestock Science 162, 178–184.Google Scholar
Saura-Calixto, F 2011. Dietary fiber as a carrier of antioxidants: an essential physiological function. Journal of Agricultural and Food Chemistry 59, 43–49.Google Scholar
Sgorlon, S, Stradaioli, G, Stefanon, B, Altimer, G and Della Loggia, R 2005. Dietary grape polyphenols modulate oxidative stress in ageing rabbits. Italian Journal of Animal Science 4, 541–543.Google Scholar
Silva, VK, Morita, VD and Boleli, IC 2013. Effect of pectin extracted from citrus pulp on digesta characteristics and nutrient digestibility in broilers chickens. Revista Brasileira de Zootecnia 42, 575–583.Google Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA 1991. Methods for dietary fiber, neutral detergent fibre, and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3591.Google Scholar
Villamide, MJ. 1996. Methods of energy evaluation of feed ingredients for rabbits and their accuracy. Animal Feed Science and Technology 57, 211–223.Google Scholar
Villamide, MJ, Martens, L, Cervera, C, Perez, JM and Xiccato, G 2001. A critical approach of the calculation procedures to be used in digestibility determination of feed ingredients for rabbits. World Rabbit Science 9, 19–25.Google Scholar
Zeng, B, Han, S, Wang, P, Wen, B, Jian, W, Guo, W, Yu, Z, Du, D, Fu, X, Kong, F, Yang, M, Si, X, Zhao, J and Li, Y 2015. The bacterial communities associated with fecal types and body weight of rex rabbits. Scientific Reports 5, 9342.Google Scholar
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