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Wool cortisol is a better indicator of stress than blood cortisol in ewes exposed to heat stress and water restriction

Published online by Cambridge University Press:  04 November 2013

J. Ghassemi Nejad ,
J. D. Lohakare ,
J. K. Son ,
E. G. Kwon ,
J. W. West  and
K. I. Sung
J. Ghassemi Nejad
Affiliation:
College of Animal Life Sciences, Kangwon National University, KNU Ave. 1, Chuncheon 200-701, Gangwon, South Korea
J. D. Lohakare
Affiliation:
College of Animal Life Sciences, Kangwon National University, KNU Ave. 1, Chuncheon 200-701, Gangwon, South Korea
J. K. Son
Affiliation:
National Institutes of Animal Science, RDA, Cheonan 331-801, South Korea
E. G. Kwon
Affiliation:
National Institutes of Animal Science, RDA, Cheonan 331-801, South Korea
J. W. West
Affiliation:
Department of Animal and Dairy Science, University of Georgia, 31793-0748 Tifton, USA
K. I. Sung*
Affiliation:
College of Animal Life Sciences, Kangwon National University, KNU Ave. 1, Chuncheon 200-701, Gangwon, South Korea
*
† E-mail: [email protected]
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Abstract

This study investigated the effect of water restriction on wool and blood cortisol concentrations and water consumption patterns in heat-stressed sheep. Nine Corriedale female sheep (average BW=43±6.5 kg) were individually fed diets based on maintenance requirement in metabolic crates. They were assigned to three treatments according to a Latin square design (3×3) for three periods with a 21-day duration for each period (nine sheep per treatment). Treatments included free access to water (FAW), 2 h water restriction (2hWR) and 3 h water restriction (3hWR) after feeding. Average temperature–humidity index in the experimental room was 27.9 throughout the experiment that defines heat stress conditions. Wool samples were taken at the end of each period on day 21. No differences were found in cortisol concentration in each fragment (dried, washed and residual extract) of wool (P<0.05). Total wool cortisol concentration was higher in the 3hWR group than the other treatments (P<0.05). Blood cortisol was not different among the treatments (P>0.05) and resulted in higher variable data compared with wool cortisol. Blood neutrophils and neutrophil/lymphocyte ratio suppressed in FAW and 3hWR groups compared with the 2hWR group (P<0.05). The duration of water consumption recorded after feeding in the 3hWR group was higher than in the 2hWR group when recorded in the afternoon (P<0.01). Water consumption rate was higher in the 3hWR group than in the 2hWR group (P<0.01). However, total water consumed was lower in the 3hWR group compared with other treatments (P>0.05). It can be concluded that wool cortisol provides more precise and accurate data than blood cortisol during heat stress conditions. Water restriction for 3 h after feeding can act as a stressor and is critical for sheep during heat stress as the consumption of water decreases with restriction.

Keywords

sheepwool cortisolheat stresswater restrictionblood cortisol
Type
Full Paper
Information
animal , Volume 8 , Issue 1 , January 2014 , pp. 128 - 132
Copyright
Copyright © The Animal Consortium 2013 

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References

Accorsi, PA, Carloni, E, Valsecchi, P, Viggiani, R, Gamberoni, M, Tamanini, C and Seren, E 2008. Cortisol determination in hair and faeces from domestic cats and dogs. General and Comparative Endocrinology 155, 398–402.Google Scholar
Bennett, A and Hayssen, V 2010. Measuring cortisol in hair and saliva from dogs: coat color and pigment differences. Domestic Animal Endocrinology 39, 171–180.Google Scholar
Boandl, KE, Wohlt, JE and Carsia, RV 1989. Effects of handling, administration of a local anesthetic, and electrical dehorning on plasma cortisol in Holstein calves. Journal of Dairy Science 72, 2193–2197.Google Scholar
Casamassima, D, Pizzo, R, Palazzo, M, DʼAlessandro, AG and Martemucci, G 2008. Effect of water restriction on productive performance and blood parameters in comisana sheep reared under intensive condition. Small Ruminant Research 78, 169–175.Google Scholar
Comin, A, Veronesi, MC, Montillo, M, Faustini, M, Valentini, S, Cairoli, F and Prandi, A 2012. Hair cortisol level as a retrospective marker of hypothalamic-pituitary-adrenal axis activity in horse foals. The Veterinary Journal 194, 131–132.Google Scholar
Craven, N and Williams, MR 1985. Defences of the bovine mammary gland against infection and prospects for their enhancement. Veterinary Immunology and Immunopathology 10, 71–127.Google Scholar
Creel, S, Creel, NM, Wildt, DE and Monfort, SL 1992. Behavioural and endocrine mechanisms of reproductive suppression in Serengeti dwarf mongooses. Animal Behaviour 43, 231–245.Google Scholar
Davenport, MD, Tiefenbacher, S, Lutz, CK, Novak, MA and Meyer, JS 2006. Analysis of endogenous cortisol levels in the hair of rhesus macaques. General Comparative Endocrinology 147, 255–261.Google Scholar
Kalra, S, Einarson, A, Karaskov, T, Van Uum, S and Koren, G 2007. The relationship between stress and hair cortisol in healthy pregnant women. Clinical & Investigative Medicine 30, 103–107.Google Scholar
Kohen, F, Pazzagli, M, Kim, JB and Lindner, HR 1980. An immunoassay for plasma cortisol based on chemiluminescence. Steroids 36, 421–437.Google Scholar
Li, BT, Christopherson, RJ and Cosgrove, SJ 2000. Effect of water restriction and environmental temperatures on metabolic rate and physiological parameters in sheep. Canadian Journal of Animal Science 80, 97–104.Google Scholar
Maria, IFM, El-Darawany, AA, Fadiel, A and Abdel-Hafez, MAM 2007. Physiological traits as affected by heat stress in sheep – a review. Small Ruminant Research 71, 1–12.Google Scholar
Paulsen, RB, Wilkins, DG, Slawson, MH, Shaw, K and Rollins, DE 2001. Effect of four laboratory decontamination procedures on the quantitative determination of cocaine and metabolites in hair by HPLC–MS. Journal of Analytical Toxicology 25, 490–496.Google Scholar
Silanikov, E 1987. Effect of imposed reduction of energy intake of resting and fasting heat production on black Bedouin goats. Nutrition Report International 35, 725–731.Google Scholar
Yamada, J, Stevens, B, de Silva, N, Gibbins, S, Beyene, J, Taddio, A, Newman, C and Koren, G 2007. Hair cortisol as a potential biologic marker of chronic stress in hospitalized neonates. Neonatology 92, 42–49.Google Scholar