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Whole-transcriptome profiling of sheep fed with a high iodine-supplemented diet

Published online by Cambridge University Press:  23 October 2019

M. Iannaccone Open the ORCID record for M. Iannaccone [Opens in a new window] ,
R. Elgendy Open the ORCID record for R. Elgendy [Opens in a new window] ,
A. Ianni ,
C. Martino ,
F. Palazzo ,
M. Giantin Open the ORCID record for M. Giantin [Opens in a new window] ,
L. Grotta ,
M. Dacasto Open the ORCID record for M. Dacasto [Opens in a new window]  and
G. Martino Open the ORCID record for G. Martino [Opens in a new window]
M. Iannaccone
Affiliation:
Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, Via R. Balzarini 1, 64100Teramo, Italy
R. Elgendy
Affiliation:
Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell’Università 16, 35020Legnaro, Italy
A. Ianni
Affiliation:
Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, Via R. Balzarini 1, 64100Teramo, Italy
C. Martino
Affiliation:
Specialist Diagnostic Department, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Via Campo Boario, 64100 Teramo (TE), Italy
F. Palazzo
Affiliation:
Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, Via R. Balzarini 1, 64100Teramo, Italy
M. Giantin
Affiliation:
Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell’Università 16, 35020Legnaro, Italy
L. Grotta
Affiliation:
Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, Via R. Balzarini 1, 64100Teramo, Italy
M. Dacasto
Affiliation:
Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell’Università 16, 35020Legnaro, Italy
G. Martino*
Affiliation:
Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, Via R. Balzarini 1, 64100Teramo, Italy
*
†E-mail: [email protected]
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Abstract

Iodine (I) is a micronutrient that mammals need for proper functionality of thyroid gland since it is the main component of thyroid hormones. Besides studies that have investigated the role of I in livestock nutrition, it is also important to know the transcriptomics changes in small ruminants following I supplementation. Therefore, the aim of this study was to investigate the effects of I on the whole blood transcriptome in sheep. Fifteen lactating cross-bred ewes (3 to 4-year-old, 55 to 65 kg BW) at their late lactation period were enrolled in this study. At the beginning, all the animals had a 2-week acclimation period where they were fed with a basal diet which includes an adequate level of I (2 mg I/animal per day) in the form of calcium iodate (CaI2O6). Then, the ewes were randomly divided into two groups and fed in individual troughs: the control group (n = 5) was maintained on basal diet and the experimental group (I, n = 10) was fed for 40 days with a diet containing a high I supplementation (equivalent to 30 mg I/animal per day), in the form of potassium iodide. Whole blood and milk were collected individually at the beginning (T0) and after the 40 days of supplementation (T40). Iodine quantification was assessed in serum and milk sample. Microarray gene expression analysis was performed on whole blood and, filtering data using a fold change >2 with an adjusted P < 0.05, we identified 250 differentially expressed genes (DEGs) in the I group (T40 v. T0). Looking for biological processes associated with our DEGs, we found significant association with cell growth regulation. Thus, our study unveils the role of I supplementation on gene expression in sheep improving the knowledge about micronutrients in animal nutrition.

Keywords

iodine supplementationmicroarraygene expressionpathway analysissheep
Type
Research Article
Information
animal , Volume 14 , Issue 4 , April 2020 , pp. 745 - 752
Copyright
© The Animal Consortium 2019 

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Footnotes

*

These authors contributed equally to this work.

a

Present address: Department of Immunology, Genetics and Pathology, Uppsala University, 75185 Uppsala, Sweden

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