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Soybean isoflavone reduces the residue of zearalenone in the muscle and liver of prepubertal gilts

Published online by Cambridge University Press:  22 November 2012

D. F. Wang ,
H. L. Zhou ,
G. Y. Hou ,
D. S. Qi  and
N. Y. Zhang
D. F. Wang
Affiliation:
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Danzhou, Hainan 571737, PR China Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China
H. L. Zhou
Affiliation:
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Danzhou, Hainan 571737, PR China
G. Y. Hou
Affiliation:
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Danzhou, Hainan 571737, PR China
D. S. Qi*
Affiliation:
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China
N. Y. Zhang
Affiliation:
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China
*
†E-mail: [email protected]
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Abstract

The aim of the present study was to evaluate the protective effects of isoflavone (ISO) against zearalenone (ZEA) residues in the muscle and liver tissues of prepubertal gilts. Seventy 75-day-old, prepubertal, female pigs (Duroc × Landrace × Yorkshire, 26.5 ± 0.60 kg) were allocated randomly to seven diet treatments for 21days as follows: one control group (fed the basal diet) and six groups fed the basal diet with the addition of either 0.5 or 2.0 mg/kg ZEA plus either 0, 300 or 600 mg/kg ISO. The results showed that the diet with 2.0 mg/kg ZEA added caused an increase of ZEA residue level in muscle tissue (P < 0.05), and that the addition of both 0.5 and 2.0 mg/kg ZEA increased the residue level of ZEA in the liver of prepubertal gilts (P < 0.05). Addition of 600 mg/kg ISO to 2.0 mg/kg ZEA-contaminated diet decreased the ZEA residue level in liver tissue (P < 0.05), and the addition of 300 or 600 mg/kg ISO to the 2.0 mg/kg ZEA-contaminated diet decreased the residue levels of ZEA in muscle tissue (P < 0.05). Western blot analysis demonstrated that feeding ZEA to prepubertal gilts increased their protein expression of 3α/3β-hydroxysteroid dehydrogenase (HSD; P < 0.05), and that the addition of 300 or 600 mg/kg ISO to the 2.0 mg/kg ZEA-contaminated diet decreased the protein expression of 3α/3β-HSD (P < 0.05), compared with the addition of 2.0 mg/kg ZEA alone. The results demonstrated that muscle and liver tissues retain residual ZEA when pigs are fed a diet contaminated with high concentrations of ZEA, and that the concentration of ZEA in muscle and liver tissues increased with increased amounts of ZEA in the feed. In diets contaminated with high levels of ZEA, the addition of ISO may accelerate the biotransformation and degradation of ZEA and its metabolites, and reduce the residues of ZEA in liver and muscle tissues of prepubertal gilts.

Keywords

soybean isoflavoneprepubertal giltszearalenone residue3α/3β-HSD expression
Type
Product quality, human health and well-being
Information
animal , Volume 7 , Issue 4 , April 2013 , pp. 699 - 703
Copyright
Copyright © The Animal Consortium 2012

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References

Biehl, ML, Prelusky, DB, Koritz, GD, Hartin, KE, Buck, WB, Trenholm, HL 1993. Biliary excretion and enterohepatic cycling of zearalenone in immature pigs. Toxicology and Applied Pharmacology 121, 152–159.Google Scholar
Binder, EM, Tan, LM, Chin, LJ, Handl, J, Richard, J 2007. Worldwide occurrence of mycotoxins in commodities, animal feed and feed ingredients. Animal Feed Science and Technology 137, 265–282.Google Scholar
Bradford, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Danicke, S, Ueberschar, KH, Halle, I, Matthes, S, Valenta, H, Flachowsky, G 2002. Effect of addition of a detoxifying agent to laying hen diets containing uncontaminated or Fusarium toxin-contaminated maize on performance of hens and on carryover of zearalenone. Poultry Science 81, 1671–1680.Google Scholar
Fink-Gremmels, J, Malekinejad, H 2007. Clinical effects and biochemical mechanisms associated with exposure to the mycoestrogen zearalenone. Animal Feed Science and Technology 137, 326–341.Google Scholar
Gimeno, A 1979. Thin layer chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin, and penitrem A. Journal – Association of Official Analytical Chemists 62, 579–585.Google Scholar
Glenn, AE 2007. Mycotoxigenic Fusarium species in animal feed. Animal Feed Science and Technology 137, 213–240.Google Scholar
Greiner, LL, Stahly, TS, Stabel, TJ 2001. The effect of dietary soy genistein on pig growth and viral replication during a viral challenge. Journal of Animal Science 79, 1272–1279.CrossRefGoogle ScholarPubMed
Heimler, D, Vignolini, P, Galardi, C, Pinelli, P, Romani, A 2004. Simple extraction and rapid quantitative analysis of isoflavones in soybean seeds. Chromatographia 59, 361–365.Google Scholar
Hestbjerg, H, Nielsen, KF, Thrane, U, Elmholt, S 2002. Production of trichothecenes and other secondary metabolites by Fusarium culmorum and Fusarium equiseti on common laboratory media and a soil organic matter agar: an ecological interpretation. Journal of Agricultural and Food Chemistry 50, 7593–7599.Google Scholar
Hsieh, HM, Wu, WM, Hu, ML 2009. Soy isoflavones attenuate oxidative stress and improve parameters related to aging and Alzheimer's disease in C57BL/6J mice treated with d-galactose. Food and Chemical Toxicology 47, 625–632.Google Scholar
Hwang, CS, Kwak, HS, Lim, HJ, Lee, SH, Kang, YS, Choe, TB, Hur, HG, Han, KO 2006. Isoflavone metabolites and their in vitro dual functions: they can act as an estrogenic agonist or antagonist depending on the estrogen concentration. Journal of Steroid Biochemistry and Molecular Biology 101, 246–253.Google Scholar
James, LJ, Smith, TK 1982. Effect of dietary alfalfa on zearalenone toxicity and metabolism in rats and swine. Journal of Animal Science 55, 110–118.Google Scholar
Krska, R, Welzig, E, Boudra, H 2007. Analysis of Fusarium toxins in feed. Animal Feed Science and Technology 137, 241–264.CrossRefGoogle Scholar
Kuiper-Goodman, T, Scott, PM, Watanbe, H 1987. Risk assessment of the mycotoxin zearalenone. Regulatory Toxicology Pharmacology 7, 253–306.Google Scholar
Malekinejad, H, Maas-Bakker, R, Fink-Gremmels, J 2006. Species differences in the hepatic biotransformation of zearalenone. Veterinary Journal 172, 96–102.Google Scholar
Mirocha, CJ, Pathre, SV, Robison, TS 1981. Comparative metabolism of zearalenone and transmission into bovine milk. Food and Cosmetics Toxicology 19, 25–30.Google Scholar
Mirocha, CJ, Robison, TS, Pawlosky, RJ, Allen, NK 1982. Distribution and residue determination of [3H]zearalenone in broilers. Toxicology and Applied Pharmacology 66, 77–87.Google Scholar
NRC 1998. NRC, nutrient requirements of swine, 10th revised edition. National Academy Press, Washington, DC, USA.Google Scholar
Olsen, M, Pettersson, H, Kiessling, KH 1981. Reduction of zearalenone to zearalenol in female rat liver by 3 alpha-hydroxysteroid dehydrogenase. Acta Pharmacological Toxicology 48, 157–161.Google Scholar
Omoni, AO, Aluko, RE 2005. Soybean foods and their benefits: potential mechanisms of action. Nutrition Reviews 63, 272–283.Google Scholar
Payne, RL, Bidner, TD, Southern, LL, Geaghan, JP 2001. Effects of dietary soy isoflavones on growth, carcass traits, and meat quality in growing-finishing pigs. Journal of Animal Science 79, 1230–1239.Google Scholar
Reinli, K, Block, G 1996. Phytoestrogen content of foods – a compendium of literature values. Nutrition and Cancer – An International Journal 26, 123–148.Google Scholar
SAS Institute 1990. SAS/STAT® user's guide, Version 6, 4th edition. SAS Institute, Cary, NC, USA.Google Scholar
Wang, DF, Zhang, NY, Peng, YZ, Qi, DS 2010. Interaction of zearalenone and soybean isoflavone on the development of reproductive organs, reproductive hormones and estrogen receptors expression in prepubertal gilts. Animal Reproduction Science 122, 317–323.Google Scholar
Wang, HJ, Murphy, PA 1994. Isoflavone composition of American and Japanese soybeans in Iowa: effects of variety, crop year, and location. Journal of Agricultural and Food Chemistry 42, 1674–1677.Google Scholar
Xu, YC, Zhang, GS, Chu, FS 1988. Enzyme-linked immunosorbent assay for deoxynivalenol in corn and wheat. Journal – Association of Official Analytical Chemists 71, 945–949.Google Scholar
Yamashita, A, Yoshizawa, T, Aiura, Y, Sanchez, PC, Dizon, EI, Arim, RH, Sardjono 1995. Fusarium mycotoxins (fumonisins, nivalenol, and zearalenone) and aflatoxins in corn from Southeast Asia. Bioscience Biotechnology and Biochemistry 59, 1804–1807.Google Scholar
Zinedine, A, Soriano, JM, Molto, JC, Manes, J 2007. Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. Food Chemistry and Toxicology 45, 1–18.Google Scholar
Zollner, P, Jodlbaure, J, Kleinova, M, Kahlbacher, H, Kuhn, T, Hochsteiner, W, Lindner, W 2002. Concentration levels of zearalenone and its metabolites in urine, muscle tissue, and liver samples of pigs fed with mycotoxin-contaminated oats. Journal of Agricultural and Food Chemistry 50, 2494–2501.Google Scholar