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Maternal dietary supplementation with ferrous N-carbamylglycinate chelate affects sow reproductive performance and iron status of neonatal piglets

Published online by Cambridge University Press:  27 November 2017

D. Wan ,
Y. M. Zhang ,
X. Wu ,
X. Lin ,
X. G. Shu ,
X. H. Zhou ,
H. T. Du ,
W. G. Xing ,
H. N. Liu  and
L. Li
...Show all authors
D. Wan
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China Guangzhou Tanke BIO-TECH Co. Ltd, Guangzhou, Guangdong 510800, China
Y. M. Zhang
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha, Hunan 410125, China
X. Wu*
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China
X. Lin
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China
X. G. Shu
Affiliation:
Guangzhou Tanke BIO-TECH Co. Ltd, Guangzhou, Guangdong 510800, China College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China
X. H. Zhou
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China
H. T. Du
Affiliation:
Shandong Newhope-Liuhe Group Company Academicion Expert Workstation, Shandong Newhope-Liuhe Group Co. Ltd, Shandong 266100, China
W. G. Xing
Affiliation:
Shandong Newhope-Liuhe Group Company Academicion Expert Workstation, Shandong Newhope-Liuhe Group Co. Ltd, Shandong 266100, China
H. N. Liu
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China
L. Li
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China
Y. Li
Affiliation:
Shandong Newhope-Liuhe Group Company Academicion Expert Workstation, Shandong Newhope-Liuhe Group Co. Ltd, Shandong 266100, China
Y. L. Yin*
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha, Hunan 410125, China
*
†E-mail: [email protected], [email protected]
†E-mail: [email protected], [email protected]
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Abstract

Iron-deficiency anemia is a public health concern that frequently occurs in pregnant mammals and neonatal offspring. Ferrous N-carbamylglycinate chelate (Fe-CGly) is a newly designed iron fortifier with proven effects in iron-deficient rats and weanling piglets. However, the effects of this new compound on pregnant mammals are unknown. Therefore, this experiment was conducted to evaluate the effects of Fe-CGly on sow reproductive performance and iron status of both sows and neonatal piglets. A total of 40 large-white sows after second parity were randomly assigned to two groups (n=20). They were receiving a diet including 80 mg Fe/kg as FeSO4 or Fe-CGly, respectively, from day 85 of gestation to parturition. The serum (day 110 of pregnancy) and placentas of sows were sampled. Litter size, mean weight of live born piglets, birth (live) litter weight, number of live born piglets, and the number of still-born piglets, mummies, and weak-born piglets were recorded. Once delivered, eight litters were randomly selected from the 20 litters per treatment, and one new-born male piglet (1.503±0.142 kg) from each selected litter was slaughtered within 3 h after birth from the selected litters, without colostrum ingestion. The serum, longissimus muscle, liver and kidneys of the piglets were collected. The iron status of the serum samples and the messenger RNA level of iron-related genes in the placenta, liver and kidney were analyzed. The results showed that litter weight of live born piglets was higher (P=0.030) in the Fe-CGly group (19.86 kg) than in the FeSO4 group (17.34 kg). Fe-CGly significantly increased placental iron concentration (P<0.05) of sows. It also significantly increased iron saturation and reduced the total iron-binding capacity of piglets (P<0.05) at birth. However, the results revealed that supplementation of Fe-CGly in sows reduced liver and kidney iron concentration of neonatal piglets (P<0.05), indicating decreased iron storage. In addition, the concentration of iron in the colostrum was not significantly changed. Therefore, the present results suggested that replacement of maternal FeSO4 supplement with Fe-CGly in the late-gestating period for sows could improve litter birth weight, probably via enhanced iron transportation in the placenta.

Keywords

ferrous N-carbamylglycinate chelatesowsneonatal pigletsreproductive performanceiron status
Type
Research Article
Information
animal , Volume 12 , Issue 7 , July 2018 , pp. 1372 - 1379
Copyright
© The Animal Consortium 2017 

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References

Association of Official Analytical Chemists 2004. Official methods of analysis, volume 2, 18th edition. AOAC, Arlington, VA, USA.Google Scholar
Berglund, S, Westrup, B and Domellöf, M 2010. Iron supplements reduce the risk of iron deficiency anemia in marginally low birth weight infants. Pediatrics 126, e874–e883.Google Scholar
Berglund, SK, Westrup, B and Domellöf, M 2015. Iron supplementation until 6 months protects marginally low-birth-weight infants from iron deficiency during their first year of life. Journal of Pediatric Gastroenterology and Nutrition 60, 390–395.Google Scholar
Brady, PS, Ku, PK, Ullrey, DE and Miller, ER 1978. Evaluation of an amino acid-iron chelate hematinic for the baby pig. Journal of Animal Science 47, 1135–1140.Google Scholar
Chacher, B, Liu, H, Wang, D and Liu, J 2013. Potential role of N-carbamoyl glutamate in biosynthesis of arginine and its significance in production of ruminant animals. Journal of Animal Science and Biotechnology 4, 16–22.CrossRefGoogle ScholarPubMed
Choudhury, V, Amin, SB, Agarwal, A, Srivastava, LM, Soni, A and Saluja, S 2015. Latent iron deficiency at birth influences auditory neural maturation in late preterm and term infants. The American Journal of Clinical Nutrition 102, 1030–1034.Google Scholar
Duque, X, Martinez, H, Vilchis-Gil, J, Mendoza, E, Flores-Hernandez, S, Moran, S, Navarro, F, Roque-Evangelista, V, Serrano, A and Mera, RM 2014. Effect of supplementation with ferrous sulfate or iron bis-glycinate chelate on ferritin concentration in Mexican schoolchildren: a randomized controlled trial. The Journal of Nutrition 13, 1–10.Google Scholar
Egeli, AK and Framstad, T 1997. Effect of an oral starter dose of iron on haematology and weight gain in piglets having voluntary access to glutamic acid-chelated iron solution. Acta Veterinaria Scandinavica 39, 359–365.Google Scholar
Frank, JW, Escobar, J, Nguyen, HV, Jobgen, SC, Davis, TA and Wu, G 2006. Oral Ncarbamylglutamate (NCG) supplementation increases growth rate in sow-reared pigets. FASEB J 20, A425.Google Scholar
Frank, JW, Escobar, J, Nguyen, HV, Jobgen, SC, Jobgen, WS, Davis, TA and Wu, G 2007. Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets. The Journal of Nutrition 137, 315–319.Google Scholar
Georgieff, MK, Berry, SA, Wobken, JD and Leibold, EA 1999. Increased placental iron regulatory protein-1 expression in diabetic pregnancies complicated by fetal iron deficiency. Placenta 20, 87–93.CrossRefGoogle ScholarPubMed
Gruper, Y, Bar, J, Bacharach, E and Ehrlich, R 2005. Transferrin receptor co‐localizes and interacts with the hemochromatosis factor (HFE) and the divalent metal transporter-1 (DMT1) in trophoblast cells. Journal of Cellular Physiology 204, 901–912.Google Scholar
Gunshin, H, Fujiwara, Y, Custodio, AO, DiRenzo, C, Robine, S and Andrews, NC 2005. Slc11a2 is required for intestinal iron absorption and erythropoiesis but dispensable in placenta and liver. The Journal of Clinical Investigation 115, 1258–1266.Google Scholar
Gunshin, H, Mackenzie, B, Berger, UV, Gunshin, Y, Romero, MF, Boron, WF, Nussberger, S, Gollan, JL and Hediger, MA 1997. Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388, 482–488.Google Scholar
Haider, BA, Olofin, I, Wang, M, Spiegelman, D, Ezzati, M and Fawzi, WW 2013. Anaemia, prenatal iron use, and risk of adverse pregnancy outcomes: systematic review and meta-analysis. BMJ 346, f3443.Google Scholar
Hostetler, CE, Kincaid, RL and Mirando, MA 2003. The role of essential trace elements in embryonic and fetal development in livestock. The Veterinary Journal 166, 125–139.Google Scholar
Klausner, RD, Ashwell, G, Van Renswoude, J, Harford, JB and Bridges, KR 1983. Binding of apotransferrin to K562 cells: explanation of the transferrin cycle. Proceedings of the National Academy of Sciences 80, 2263–2266.Google Scholar
Klobasa, F, Werhahn, E and Butler, JE 1987. Composition of sow milk during lactation. Journal of Animal Science 64, 1458–1466.Google Scholar
Lind, T, Lönnerdal, B, Stenlund, H, Ismail, D, Seswandhana, R, Ekström, EC and Persson, LÅ 2003. A community-based randomized controlled trial of iron and zinc supplementation in Indonesian infants: interactions between iron and zinc. The American Journal of Clinical Nutrition 77, 883–890.Google Scholar
McArdle, HJ, Andersen, HS, Jones, H and Gambling, L 2008. Copper and iron transport across the placenta: regulation and interactions. Journal of Neuroendocrinology 20, 427–431.Google Scholar
Milman, N, Jønsson, L, Dyre, P, Pedersen, PL and Larsen, LG 2014. Ferrous bisglycinate 25 mg iron is as effective as ferrous sulfate 50 mg iron in the prophylaxis of iron deficiency and anemia during pregnancy in a randomized trial. Journal of Perinatal Medicine 42, 197–206.Google Scholar
Moore, RW, Redmond, HE and Livingston, JCW 1965. Iron deficiency anemia as a cause of stillbirths in swine. Journal of the American Veterinary Medical Association 147, 746–748.Google Scholar
Muehlenbein, E, Brink, D, Deutscher, G, Carlson, M and Johnson, A 2001. Effects of inorganic and organic copper supplemented to first-calf cows on cow reproduction and calf health and performance. Journal of Animal Science 79, 1650–1659.Google Scholar
National Research Council 2012. Nutrient requirements of swine. 11th revised edition. The National Academy Press, Washington, DC, USA.Google Scholar
Peters, JC and Mahan, DC 2008. Effects of neonatal iron status, iron injections at birth, and weaning in young pigs from sows fed either organic or inorganic trace minerals. Journal of Animal Science 86, 2261–2269.Google Scholar
Rossander-Hultén, L, Brune, M, Sandström, B, Lönnerdal, B and Hallberg, L 1991. Competitive inhibition of iron absorption by manganese and zinc in humans. The American Journal of Clinical Nutrition 54, 152–156.Google Scholar
Sandström, B 2001. Micronutrient interactions: effects on absorption and bioavailability. British Journal of Nutrition 85 (S2), S181–S185.CrossRefGoogle ScholarPubMed
Scholl, TO, Hediger, ML, Fischer, RL and Shearer, JW 1992. Anemia vs iron deficiency: increased risk of preterm delivery in a prospective study. The American Journal of Clinical Nutrition 55, 985–988.Google Scholar
Spears, JW, Schoenherr, WD, Kegley, EB, Flowers, WL and Alhunsen, HD 1992. Efficacy of iron methionine as a source of iron for nursing pigs. Journal of Animal Science 70 (suppl. 1), 243.Google Scholar
Stoltzfus, RJ 2003. Iron deficiency: global prevalence and consequences. Food and Nutrition Bulletin 24 (suppl. 2), S99–S103.Google Scholar
Tummaruk, P, Tantilertcharoen, R, Pondeenana, S, Buabucha, P and Virakul, P 2003. Effect of an iron glycine chelate supplement on the haemoglobin and the haematocrit values and reproductive traits of sows. The Thai Journal of Veterinary Medicine 33, 45–53.Google Scholar
Walker, CF, Kordas, K, Stoltzfus, RJ and Black, RE 2005. Interactive effects of iron and zinc on biochemical and functional outcomes in supplementation trials. The American Journal of Clinical Nutrition 82, 5–12.Google Scholar
Wan, D, Zhou, X, Xie, C, Shu, X, Wu, X and Yin, Y 2015. Toxicological evaluation of ferrous N-carbamylglycinate chelate: acute, sub-acute toxicity and mutagenicity. Regulatory Toxicology and Pharmacology 73, 644–651.Google Scholar
Wang, J, Li, D, Che, L, Lin, Y, Fang, Z, Xu, S and Wu, D 2014. Influence of organic iron complex on sow reproductive performance and iron status of nursing pigs. Livestock Science 160, 89–96.Google Scholar
Wei, KQ, Xu, ZR, Luo, XG, Zeng, LL, Chen, WR and Timothy, MF 2005. Effects of iron from an amino acid complex on the iron status of neonatal and suckling piglets. Asian Australasian Journal of Animal Sciences 18, 1485–1491.Google Scholar
Wu, G, Knabe, DA and Kim, SW 2004. Arginine nutrition in neonatal pigs. The Journal of Nutrition 134, 2783–2790.Google Scholar
Wu, X, Wan, D, Xie, C, Li, T, Huang, R, Shu, X, Ruan, Z, Deng, Z and Yin, Y 2015. Acute and sub-acute oral toxicological evaluations and mutagenicity of N-carbamylglutamate (NCG). Regulatory Toxicology and Pharmacology 73, 296–302.Google Scholar
Zaleski, HM and Hacker, RR 1993. Variables related to the progress of parturition and probability of stillbirth in swine. The Canadian Veterinary Journal 34, 109–113.Google Scholar
Zhang, Y, Sun, X, Xie, C, Shu, X, Oso, AO, Ruan, Z, Deng, Z, Wu, X and Yin, Y 2015. Effects of ferrous carbamoyl glycine on iron state and absorption in an iron-deficient rat model. Gene Nutrition 10, 1–8.Google Scholar