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Lean and obese pig breeds exhibit differences in prenatal gene expression profiles of muscle development

Published online by Cambridge University Press:  17 September 2014

X. R. Yang ,
B. Yu ,
X. B. Mao ,
P. Zheng ,
J. He ,
J. Yu ,
Y. He ,
J. M. Reecy  and
D. W. Chen
X. R. Yang
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
B. Yu
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
X. B. Mao
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
P. Zheng
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
J. He
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
J. Yu
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
Y. He
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
J. M. Reecy
Affiliation:
Department of Animal Science, Iowa State University, Ames, IA 50010, USA
D. W. Chen*
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
*
†E-mail: [email protected]
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Abstract

Muscle development in domesticated animals is important for meat production. Furthermore, intramuscular fat content is an important trait of meat intended for consumption. Here, we examined differences in the expression of factors related to myogenesis, adipogenesis and skeletal muscle growth during fetal muscle development of lean (Yorkshire) and obese (Chenghua) pig breeds. At prenatal days 50 (d50) and 90 (d90), muscles and sera were collected from pig fetuses. Histology revealed larger diameters and numbers of myofibers in Chenghua pig fetuses than those in Yorkshire pig fetuses at d50 and d90. Yorkshire fetuses had higher serum concentrations of myostatin (d90), a negative regulator for muscle development, and higher mRNA expression of the growth hormone receptor Ghr (d90), myogenic MyoG (d90) and adipogenic LPL (d50). By contrast, Chenghua fetuses exhibited higher serum concentration of growth hormone (d90), and higher mRNA expression of myogenic MyoD (d90) as well as adipogenic PPARG and FABP4 (d50). Our results revealed distinct expression patterns in the two pig breeds at each developmental stage before birth. Compared with Chenghua pigs, development and maturation of fetal skeletal muscles may occur earlier in Yorkshire pigs, but the negative regulatory effects of myostatin may suppress muscle development at the later stage.

Keywords

breed differencepigfetal muscle developmentmyostatinmyogenesis
Type
Research Article
Information
animal , Volume 9 , Supplement 1 , January 2015 , pp. 28 - 34
Copyright
© The Animal Consortium 2014 

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References

Beermann, DH, Cassens, RG and Hausman, GJ 1978. A second look at fiber type differentiation in porcine skeletal muscle. Journal of Animal Science 46, 125–132.CrossRefGoogle ScholarPubMed
Bellinger, DA, Merricks, EP and Nichols, TC 2006. Swine models of type 2 diabetes mellitus: insulin resistance, glucose tolerance, and cardiovascular complications. ILAR journal/National Research Council, Institute of Laboratory Animal Resources 47, 243–258.CrossRefGoogle ScholarPubMed
da Costa, AS, Pires, VM, Fontes, CM and Mestre Prates, JA 2013. Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets. BMC Veterinary Research 9, 118.CrossRefGoogle ScholarPubMed
Du, M, Yan, X, Tong, JF, Zhao, J and Zhu, MJ 2010. Maternal obesity, inflammation, and fetal skeletal muscle development. Biology of Reproduction 82, 4–12.CrossRefGoogle ScholarPubMed
Du, M, Zhao, JX, Yan, X, Huang, Y, Nicodemus, LV, Yue, W, McCormick, RJ and Zhu, MJ 2011. Fetal muscle development, mesenchymal multipotent cell differentiation, and associated signaling pathways. Journal of Animal Science 89, 583–590.CrossRefGoogle ScholarPubMed
Fahey, AJ, Brameld, JM, Parr, T and Buttery, PJ 2005. Ontogeny of factors associated with proliferation and differentiation of muscle in the ovine fetus. Journal of Animal Science 83, 2330–2338.CrossRefGoogle ScholarPubMed
Foxcroft, GR, Dixon, WT, Novak, S, Putman, CT, Town, SC and Vinsky, MD 2006. The biological basis for prenatal programming of postnatal performance in pigs. Journal of Animal Science 84 (suppl.), E105–E112.CrossRefGoogle ScholarPubMed
Gao, F, Kishida, T, Ejima, A, Gojo, S and Mazda, O 2013. Myostatin acts as an autocrine/paracrine negative regulator in myoblast differentiation from human induced pluripotent stem cells. Biochemical and Biophysical Research Communications 431, 309–314.CrossRefGoogle ScholarPubMed
Guernec, A, Berri, C, Chevalier, B, Wacrenier-Cere, N, Le Bihan-Duval, E and Duclos, MJ 2003. Muscle development, insulin-like growth factor-I and myostatin mRNA levels in chickens selected for increased breast muscle yield. Growth Hormone & IGF Research: Official Journal of the Growth Hormone Research Society and the International IGF Research Society 13, 8–18.CrossRefGoogle ScholarPubMed
Guo, T, Bond, ND, Jou, W, Gavrilova, O, Portas, J and McPherron, AC 2012. Myostatin inhibition prevents diabetes and hyperphagia in a mouse model of lipodystrophy. Diabetes 61, 2414–2423.CrossRefGoogle Scholar
Hyatt, JP, McCall, GE, Kander, EM, Zhong, H, Roy, RR and Huey, KA 2008. PAX3/7 expression coincides with MyoD during chronic skeletal muscle overload. Muscle & Nerve 38, 861–866.CrossRefGoogle ScholarPubMed
Liu, W, Thomas, SG, Asa, SL, Gonzalez-Cadavid, N, Bhasin, S and Ezzat, S 2003. Myostatin is a skeletal muscle target of growth hormone anabolic action. The Journal of Clinical Endocrinology and Metabolism 88, 5490–5496.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402–408.CrossRefGoogle ScholarPubMed
Marcell, TJ, Harman, SM, Urban, RJ, Metz, DD, Rodgers, BD and Blackman, MR 2001. Comparison of GH,IGF-I, and testosterone with mRNA of receptors and myostatin in skeletal muscle in older men. American Journal of Physiology. Endocrinology and Metabolism 281, E1159–E1164.CrossRefGoogle ScholarPubMed
McPherron, AC and Lee, SJ 1997. Double muscling in cattle due to mutations in the myostatin gene. Proceedings of the National Academy of Sciences of the United States of America 94, 12457–12461.CrossRefGoogle ScholarPubMed
McPherron, AC and Lee, SJ 2002. Suppression of body fat accumulation in myostatin-deficient mice. The Journal of Clinical Investigation 109, 595–601.CrossRefGoogle ScholarPubMed
McPherron, AC, Lawler, AM and Lee, SJ 1997. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 387, 83–90.CrossRefGoogle ScholarPubMed
Megeney, LA and Rudnicki, MA 1995. Determination versus differentiation and the MyoD family of transcription factors. Biochemistry and Cell Biology 73, 723–732.CrossRefGoogle ScholarPubMed
Natarajan, R, Gerrity, RG, Gu, JL, Lanting, L, Thomas, L and Nadler, JL 2002. Role of 12-lipoxygenase and oxidant stress in hyperglycaemia-induced acceleration of atherosclerosis in a diabetic pig model. Diabetologia 45, 125–133.CrossRefGoogle Scholar
Patruno, M, Caliaro, F, Maccatrozzo, L, Sacchetto, R, Martinello, T, Toniolo, L, Reggiani, C and Mascarello, F 2008. Myostatin shows a specific expression pattern in pig skeletal and extraocular muscles during pre- and post-natal growth. Differentiation 76, 168–181.CrossRefGoogle Scholar
Perrini, S, Laviola, L, Carreira, MC, Cignarelli, A, Natalicchio, A and Giorgino, F 2010. The GH/IGF1 axis and signaling pathways in the muscle and bone: mechanisms underlying age-related skeletal muscle wasting and osteoporosis. The Journal of Endocrinology 205, 201–210.CrossRefGoogle ScholarPubMed
Rosen, ED, Sarraf, P, Troy, AE, Bradwin, G, Moore, K, Milstone, DS, Spiegelman, BM and Mortensen, RM 1999. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Molecular Cell 4, 611–617.CrossRefGoogle ScholarPubMed
Soslow, RA, Dannenberg, AJ, Rush, D, Woerner, BM, Khan, KN, Masferrer, J and Koki, AT 2000. COX-2 is expressed in human pulmonary, colonic, and mammary tumors. Cancer 89, 2637–2645.3.0.CO;2-B>CrossRefGoogle ScholarPubMed
Suzuki, LA, Poot, M, Gerrity, RG and Bornfeldt, KE 2001. Diabetes accelerates smooth muscle accumulation in lesions of atherosclerosis: lack of direct growth-promoting effects of high glucose levels. Diabetes 50, 851–860.CrossRefGoogle ScholarPubMed
Te Pas, MFW, Everts, ME and Haagsman, HP 2004. Muscle development of livestock animals: physiology, genetics, and meat quality. CABI Publishing, Cambridge, MA, USA.CrossRefGoogle Scholar
Verner, J, Humpolicek, P and Knoll, A 2007. Impact of MYOD family genes on pork traits in Large White and Landrace pigs. Journal of Animal Breeding and Genetics 124, 81–85.CrossRefGoogle ScholarPubMed
Vijayakumar, A, Wu, Y, Sun, H, Li, X, Jeddy, Z, Liu, C, Schwartz, GJ, Yakar, S and LeRoith, D 2012. Targeted loss of GHR signaling in mouse skeletal muscle protects against high-fat diet-induced metabolic deterioration. Diabetes 61, 94–103.CrossRefGoogle ScholarPubMed
Warnes, GR, Bolker, B, Bonebakker, L, Gentleman, R, Liaw, WHA, Lumley, T, Maechler, M, Magnusson, A, Moeller, S, Schwartz, M and Venables, B 2014. gplots: Various R programming tools for plotting data. R package version 2.13.0. Retrieved April 5, 2014, from http:// 539Q5 CRAN.R-project.org/package=gplots Google Scholar
Williams, NG, Interlichia, JP, Jackson, MF, Hwang, D, Cohen, P and Rodgers, BD 2011. Endocrine actions of myostatin: systemic regulation of the IGF and IGF binding protein axis. Endocrinology 152, 172–180.CrossRefGoogle ScholarPubMed
Xi, G, Hathaway, MR, Dayton, WR and White, ME 2007. Growth factor messenger ribonucleic acid expression during differentiation of porcine embryonic myogenic cells. Journal of Animal Science 85, 143–150.CrossRefGoogle ScholarPubMed
Yang, SL, Wang, ZG, Liu, B, Zhang, GX, Zhao, SH, Yu, M, Fan, B, Li, MH, Xiong, TA and Li, K 2003. Genetic variation and relationships of eighteen Chinese indigenous pig breeds. Genetics, Selection, Evolution: GSE 35, 657–671.CrossRefGoogle ScholarPubMed
Zimmerman, AW and Veerkamp, JH 2002. New insights into the structure and function of fatty acid-binding proteins. Cellular and Molecular Life Sciences: CMLS 59, 1096–1116.CrossRefGoogle ScholarPubMed
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